LOCUS       NZ_OP242316             6675 bp    DNA     circular CON 24-MAR-2024
DEFINITION  Escherichia coli strain LA207 plasmid pLAO89, complete sequence.
ACCESSION   NZ_OP242316
VERSION     NZ_OP242316.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363363
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028601005.1-RS_2024_03_24
            Annotation Date              :: 03/24/2024 01:22:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 9
            CDSs (total)                 :: 9
            Genes (coding)               :: 9
            CDSs (with protein)          :: 9
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242316.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA207"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO89"
     gene            join(5326..6675,1..204)
                     /locus_tag="PUP22_RS00005"
     CDS             join(5326..6675,1..204)
                     /locus_tag="PUP22_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="WP_000635804.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELID
                     ATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLE
                     LNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNL
                     PETKQALAEGVTRGIDALYHAGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPNGGKN
                     IRLKGAFYEQSFADGRGVREKAERESRIYRENAEQRVQEARRICKRGCDIKRDENQRRY
                     SPVHSLDRGIAGKTPGRGERGDDAAQEGRVKAGREYGHDVTGDSLSPVYREWRDALVSW
                     REDTGEPGRNQEAGRDIAETEREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTS
                     EGVTQSDRAGNTFAERLRAAATGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAV
                     ESAGAALERADRTLEPVIQRELEIREERLIQEREHVLSLERERQPEIQERTLDGPSLGW
                     "
                     /name="nuclease"
     gene            complement(415..834)
                     /locus_tag="PUP22_RS00010"
                     /old_locus_tag="CPMOAGLA_00001"
     CDS             complement(415..834)
                     /locus_tag="PUP22_RS00010"
                     /old_locus_tag="CPMOAGLA_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(915..1352)
                     /locus_tag="PUP22_RS00015"
                     /old_locus_tag="CPMOAGLA_00002"
     CDS             complement(915..1352)
                     /locus_tag="PUP22_RS00015"
                     /old_locus_tag="CPMOAGLA_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            1612..3180
                     /gene="cea"
                     /locus_tag="PUP22_RS00020"
                     /old_locus_tag="CPMOAGLA_00003"
     CDS             1612..3180
                     /gene="cea"
                     /locus_tag="PUP22_RS00020"
                     /old_locus_tag="CPMOAGLA_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(3177..3518)
                     /locus_tag="PUP22_RS00025"
                     /old_locus_tag="CPMOAGLA_00004"
     CDS             complement(3177..3518)
                     /locus_tag="PUP22_RS00025"
                     /old_locus_tag="CPMOAGLA_00004"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_000058760.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            3566..3703
                     /locus_tag="PUP22_RS00030"
                     /old_locus_tag="CPMOAGLA_00005"
     CDS             3566..3703
                     /locus_tag="PUP22_RS00030"
                     /old_locus_tag="CPMOAGLA_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(3902..4060)
                     /locus_tag="PUP22_RS00045"
     CDS             complement(3902..4060)
                     /locus_tag="PUP22_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(4758..4949)
                     /locus_tag="PUP22_RS00035"
                     /old_locus_tag="CPMOAGLA_00006"
     CDS             complement(4758..4949)
                     /locus_tag="PUP22_RS00035"
                     /old_locus_tag="CPMOAGLA_00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4998..5336
                     /locus_tag="PUP22_RS00040"
                     /old_locus_tag="CPMOAGLA_00007"
     CDS             4998..5336
                     /locus_tag="PUP22_RS00040"
                     /old_locus_tag="CPMOAGLA_00007"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001405931.1"
                     /translation="MKRERMLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSG
                     KLPTLAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVR
                     EQGARDDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          4558..4645
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(3989..4544)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.52"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5327..6675
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.778"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctgaacgag cgggtcatgc agttgagtca gcaggtgcag cacttgagcg agcagacaga
       61 acgcttgagc cagttataca gcgagaactg gagataagag aggaacggct gatacaggag
      121 cgcgaacatg tgttatccct ggaacgggag cgtcagccgg aaatacagga acgcacgctg
      181 gatggccctt cgctgggatg gtgaaaccat gaaaaatggc agcttcagtg gattaagtgg
      241 gggtaatgtg gcctgtaccc tctggttgca taggtattca tacggttaaa atttatcagg
      301 cgcgatcgcg gcagtttttc gggtggtttg ttgccatttt tacctgtctg ctgccgtgat
      361 cgcgctgaac gcgttttagc ggtgcgtaca attaagggat tatggtaaat ccacttactg
      421 tctgccctcg tagccatcga gataaaccgc agaaatcgtg tcagccagca gcctggattt
      481 tttcggggta aggtctttgc tgcggtaatc agtacccgca aattgagttg tgccccggag
      541 tgagtttaaa aattcctggc tgtgaaactg ctgaatgcgt tgttgcacat cactccgtga
      601 ttttcctgtc gctttatctt tagctcccat atcccagaac tgccgaaaaa aaggcaccat
      661 cattcgtgtt gtgtcggctt tttgggtgga aaagttggga tcaaaaccgt catcagaagc
      721 atatacgtaa tgccgggtat gttgttccac gctgcccttt ggggtacagg ctgtgagagc
      781 tactgttgct gttatcatgg cgataagaca aagtttgttt ccggtacgtc tcatgagtgt
      841 ctccctacct taaagtattt attctcggtg agttattatc cgaagactat tttatcatca
      901 gtctctgaca gcgttcagcg accgaacaac ctggaccaga atccccgttt cttttccagt
      961 tccagtgcct gcctgagctg ggctatctca ttttgtagct gttcccgttc tgcttcctgg
     1021 cgtctgcgat ccatatcctg tgcctgttta tcctcaagca tcagcgtcag gcattgtttc
     1081 agctcattca gctcccggag aatgcgttct gtctgctgat catgtggatt ttctgcatgt
     1141 ccctcactgt gcctttctgg tgtctcattc tgctttaatt cgccgtatgc ccggatcagt
     1201 tcactggttt caaactcccg tcgtccgtcc ctgccggtac ggtaactgac aaggcctgcg
     1261 cgcatatcac ggtaaaactg tgaacgcgat ctgcctgtca tttttagtgc gtcccggagt
     1321 gtgtgccatg ccataaagtg acagtgtccc atagatgtct catctcatag tttcagtaaa
     1381 acataatgag gtctgagaac ggtaatgttt gtgctggttt ttgtggcatc gggcgagaat
     1441 agcgcgtggt gtgaaagact gtttttttga tcgttttcac aaaaatggaa gtccacagtc
     1501 ttgacaggga aaatgcagcg gcgtagcttt tatgctgtat ataaaaccag tggttatatg
     1561 tacagtattt atttttaact tattgtttta aaagtcaaag aggattttat aatggaaacc
     1621 gcggtagcgt actataaaga tggtgttcct tatgatgata agggacaggt aattattact
     1681 cttttgaatg gtactcctga cgggagtggc tctggcggcg gaggtggaaa aggaggcagt
     1741 aaaagtgaaa gttctgcagc tattcatgca actgctaaat ggtctactgc tcaattaaag
     1801 aaaacacagg cagagcaggc tgcccgggca aaagctgcag cggaagcaca ggcgaaagca
     1861 aaggcaaaca gggatgcgct gactcagcgc ctgaaggata tcgtgaatga ggctcttcgt
     1921 cacaatgcct cacgtacgcc ttcagcaaca gagcttgctc atgctaataa tgcagctatg
     1981 caggcggaag cagagcgttt gcgccttgcg aaagcagaag aaaaagcccg taaagaagcg
     2041 gaagcagcag aaaaggcttt tcaggaagca gaacaacgac gtaaagagat tgaacgggag
     2101 aaggctgaaa cagaacgcca gttgaaactg gctgaagctg aagagaaacg actggctgca
     2161 ttgagtgaag aagctaaagc tgttgagatc gcccaaaaaa aactttctgc tgcacaatct
     2221 gaagtggtga aaatggatgg agagattaag actctcaatt ctcgtttaag ctccagtatc
     2281 catgcccgtg atgcagaaat gaaaacgctc gctggaaaac gaaatgaact ggctcaggca
     2341 tccgctaaat ataaagaact ggatgagctg gtcaaaaaac tatcaccaag agccaatgat
     2401 ccgcttcaga accgtccttt ttttgaagca accagacgac gggttggggc cggtaagatt
     2461 agagaagaaa aacaaaaaca ggtaacagca tcagaaacac gtattaaccg gataaatgct
     2521 gatataactc agatccagaa ggctatttct caggtcagta ataatcgtaa tgccggtatc
     2581 gctcgtgttc atgaagctga agaaaatttg aaaaaagcac agaataatct ccttaattca
     2641 cagattaagg atgctgttga tgcaacagtt agcttttatc aaacgctgac tgaaaaatat
     2701 ggtgaaaaat attcgaaaat ggcacaggaa cttgctgata agtctaaagg taagaaaatc
     2761 ggcaatgtga atgaagctct cgctgctttt gaaaaataca aggatgtttt aaataagaaa
     2821 ttcagcaaag ccgatcgtga tgctattttt aatgcgttgg catcggtgaa gtatgatgac
     2881 tgggctaaac atttagatca gtttgccaag tacttgaaga ttacggggca tgtttctttt
     2941 ggatatgatg tggtatctga tatcctaaaa attaaggata caggtgactg gaagccacta
     3001 tttcttacat tagagaagaa agctgcagat gcaggggtga gttatgttgt tgctttactt
     3061 tttagcttgc ttgctggaac tacattaggt atttggggta ttgctattgt tacaggaatt
     3121 ctatgctcct atattgataa gaataaactt aatactataa atgaggtgtt agggatttaa
     3181 ttattcttta ttgatataaa aagtcctagc aatccaaatg ggattgctag gaccaaacaa
     3241 agtagattat atagcataaa taggtttaat tttgctacgg gggcgttatt taggtttttt
     3301 cttctttcga aaaaatcttt ctttatgaag ttaaaagcta tgtattcaat agcatatttt
     3361 gaatatggac atagaatagt gcttatcact attgcatata gcatcttatc tgacacaagg
     3421 aaataatacc cttcgctgtt ttttgttata aggtatatat atataagtgt gcagtacagg
     3481 ccaaataaaa tattttttat gtagtatctt aagctcataa attaaacctc gccatatatt
     3541 cttttcattt tataaggatc gagttatgag gaaaagattt tttgtgggaa tattcgcgat
     3601 aaacctcctt gttggatgtc aggctaacta tatacgtgat gttcagggag ggaccatcgc
     3661 accatcctcc tcttctaaac tgacggggat cgcggttcag tagaaaagat caaaggatct
     3721 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3781 ccaacggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3841 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtc gggccactac
     3901 ttcaagaact ctgtagcacc gtttgtgcca tcatcgctct gctaatccgg ttaccagtgg
     3961 ctgctgccag tggcgttaag gcgtgcctta ccgggttgga ctcaagacga tagttaccgg
     4021 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4081 cgacctacac cgaaccgaga taccaacagc gtgagctatg agaaagcgcc acgcttcccg
     4141 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4201 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4261 gacttgagcg tctatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcct
     4321 gctacgtggc cttcttcctg ttcctggtct tttgctcaca tgttctttcc ggccttatcc
     4381 cctgattctg tggataactg tgttaccgtt tttgtgtgag tcagtaccgc tcgccgcagt
     4441 cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg aaaagcgcct ggacgtgcat
     4501 tttctcctta cgcatctgtg cggcatttca cacccggcat ggcgtacttt tcatacaatc
     4561 cgcactgatg ccgcatggtt aagccagtat acactccgct atcgctacgt gactgggtca
     4621 gggctgcgcc ccgacacccg ctaaaacctg ctgacgcgcc ctgacgggct tgtcagctcc
     4681 cggcatccgc tcacagacaa gctgtgaccg tctccgggag ctgcatgtct gtgagcggat
     4741 gccgggagct gcatgtgtca gaggttttca ccgtcatccc cgaaacgtgc gaggcagctg
     4801 cggtaaagct catcggcgtg gtcgtgaagc gattcacaaa tatcggcctg ttcatctgcg
     4861 tccagttcgt tgagcttctc cagcagcgtt aatgtctggc ttctgataaa gcgggccatg
     4921 ttaagggcgg ttttttcctg tttagtcact gatgcctccg tgtaaggggg atttctgttc
     4981 atggggtaat gataccgatg aaacgcgaga ggatgctcac aatacgggtt actgatgatg
     5041 aacatgcccg gttacttgaa cgctgtgagg gtaaacaact ggcggtatgg atgcggcggg
     5101 tctgcctggg ggagccggtt gcccgttccg gaaaactgcc gacactggca ccgccgttac
     5161 tgcgtcagct ggccgccatc ggaaataacc tgaatcagac agcccgtaag gtgaacagcg
     5221 ggcagtggtc ttccggtgac cgggttcagg tggtggccgc actgatggcc atcggggatg
     5281 agctgcgccg gctgcgtctg gctgtcaggg aacagggggc gcgggatgat agttaaattt
     5341 catgccaggg gaaaaggtgg tggcagtggt ccggttgatt acctgctggg gagggagcgt
     5401 aaccgcgaag gcgcaacggt gcttcagggt aatccggaag aagtccggga actcatcgat
     5461 gccacgccat ttgcgaagaa atacacgtcc ggtgttctgt cgttcgcgga gaaggagctg
     5521 ccgccgggag gacgtgaaaa agtgatggcg agctttgagc gtgtactgat gcccggtctc
     5581 gaaaaaaatc agtacagcat cctgtgggtg gagcaccagg acaagggacg gcttgagctg
     5641 aattttgtca ttccgaacat ggagctacag accggaaaac gcctccagcc gtactacgac
     5701 cgcgcagaca ggcctagaat tgatgcctgg cagacgctgg tgaatcacca ttacgggctg
     5761 catgacccga acgccccgga gaaccgcagg acgctgacac tccctgataa cctgcctgaa
     5821 acgaaacagg cgcttgctga gggcgtcacg cgaggtatag atgcacttta ccatgccgga
     5881 gagataaaag gccgtcagga tgtgattcag gcgctcactg aggcggggct ggaagtggtc
     5941 agggtgacgc gaagcagtat cagcattgca gatccgaacg gcgggaagaa tatcaggctg
     6001 aaaggagcat tttatgagca atcttttgca gacgggcgcg gagttcgaga aaaagctgaa
     6061 agagagagcc gaatctacag agaaaatgct gaacaacgag ttcaggaggc tcggcgaatc
     6121 tgtaagcgag gctgtgacat caaacgagac gaaaatcaga gacgctatag ccctgttcac
     6181 agcctcgaca gaggaatcgc tggaaaaaca ccgggaaggg gtgaaagagg cgatgatgca
     6241 gcacaggagg gacgtgttaa agctggcagg gaatacgggc atgatgttac tggggatagt
     6301 ctttctcctg tttaccgcga gtggcgggac gctctggtat cttggaggga ggatacaggc
     6361 gaacctggaa gaaatcagga agcaggaaga gacattgcag aaactgaacg cgaagacatg
     6421 gggcgtggag tttgtgcagg acgggaacag gaaattcctt gtccttccgt acgggaaatc
     6481 agcggaggtg attccctttc aggggaaaga gtgggtacat ctgaaggagt gacacagagt
     6541 gacagagctg gaaacacatt tgctgaacgc cttagagcag ctgcaacagg actatatgca
     6601 gcggctgagc gaatgggaga gcgccttcgt ggaattgcag aagatgtttt cgcttacgca
     6661 acgggacaac gcgat
//
