LOCUS       NZ_OP242315             6761 bp    DNA     circular CON 24-MAR-2024
DEFINITION  Escherichia coli strain LA207 plasmid pLAO88, complete sequence.
ACCESSION   NZ_OP242315
VERSION     NZ_OP242315.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363363
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6761)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600995.1-RS_2024_03_24
            Annotation Date              :: 03/24/2024 01:20:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 4
            CDSs (total)                 :: 4
            Genes (coding)               :: 4
            CDSs (with protein)          :: 4
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242315.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA207"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO88"
     gene            join(5852..6761,1..956)
                     /locus_tag="PUP22_RS00005"
     CDS             join(5852..6761,1..956)
                     /locus_tag="PUP22_RS00005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="WP_021558993.1"
                     /translation="MQTELIKELNKVLKAFPQFWNGEELQRSMVSDAISQKQPDLIKAL
                     VDNEKVRSVYGVDIDGIFIFDFDKLCGLLKYKEYWANSFTKYRNKVGLTSEGKYLDYSS
                     DVVLDFPFKDCILEGGMTKENQGKDEIYYNEIIAMDEVDSLLSPKAFTNAKRYTKEGVK
                     ENITKFNDDENLIIKGNNLIALHSIKKLYAGKVKLIYIDPPYNTGSDSFKYNDRFNRST
                     WLTFMKNRLEVAKEMLSDDGFIFIQCDDNESAYLKVLCDSIFGEENYRNSIYWHRTYAG
                     KTVKSKLPWNTDTILFYSKKESSLLEPYTAELSAADIKSYNKDDFDGRGKYNTVSLQKT
                     SSPGPQTTYDYIDNTGKKWSCPEKGWRMTKEKLKALENDNRLYITDKTIREKYYLKERK
                     EIGKQIDNFWHDIGNLNRVSDVAFIDGQKTEKLMQRIISLASKENDIVLDFHLGSGTSA
                     AAAHKMGRRYIGIEQMDYINEITVPRLQKVIEGEQGGISKDVNWQGSGSFVYVELVELN
                     AYFIHEIQKAQSTEELEKLFAVMKTEAYLNYQVALENVLSAEYEVDGILHKVAFSELEL
                     HEQKQLLIEILDKNQLYVNVSDMEDSDLNISESDKAFTRSFYGME"
                     /name="site-specific DNA-methyltransferase"
     gene            961..3597
                     /locus_tag="PUP22_RS00010"
                     /old_locus_tag="IAIDMIBB_00002"
     CDS             961..3597
                     /locus_tag="PUP22_RS00010"
                     /old_locus_tag="IAIDMIBB_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443154.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:0016787 -
                     hydrolase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="WP_052512605.1"
                     /translation="MAKKPKSAGREKLLFNKLKQFDEPGLFHDNYQTPDYIQANLKDIL
                     RPYQHSALRYLHYTQRKRDDALLHYRHLLFHMATGAGKTMVMAGTILYLFKEFGYQNFI
                     FFVHTDAIIQKTRENLLNPQSPKYLFSHELEIDGEKITIAPVDIFPSVPERNTIYLKLS
                     TIHKMHDELNSYRENSITYEDLKEIPLVLLGDEAHHFNAGTKARGKSKTSPENEEQTWE
                     RTIENILDLRPDNRLFEFTATIDLANKDIGQKYRDKVVYQYDLKQFMSDGYSKKVMLLE
                     ANQNDSDKMLDAVLLSQYRKLTAADHGITGFKPVILFKSNKIAISKAKQEEFSQLIAAM
                     TPESIRRHLANKKLQLSSDTSIWHKVIQRYADSDLVTVTGQIQEDFNDFNLLNVNKSDL
                     LEENPVLLNTLEEVDNPVRAVFAVAKVNEGWDVLNLYDIVRISEQASSSKTSTDSEAQL
                     IGRGARYYPFIYDGQRSFTRRFDNSTKDLSVLEQLHYHTINEPAYIKTLHASLEQADID
                     VHQDGGGTVEHARLKEDFKKSAVYQAGKLYFNEVEEIERSSRNWETYSLETRFEIQYQI
                     ACEESLDNLTGTKGGIIKPEPLVLDERFYRKAMQRISFYALDNLKRFFPKLTGIREFIR
                     SDAYLGKLKITVSVPQSLDFTTVPAKDKLHLLENVLLRISENVRRNDQKVKGTYRFISQ
                     PVKEVIKDYSLHIDPSVAINQKITAAPTIGKKWYVYDNAILNQLEHRLIKMLEAFMPKL
                     KARYDDIYVLRNDEQSTRFKLTEFGGVRGFMPDFIMILTRHSDNTYWQVFLEPKGDDRL
                     LDDAWKERMLETLNDRERIVIDENEHVRLVGIKFFANSQMDVFVSDMQNKLNDGESLET
                     SSLSLPL"
                     /name="DEAD/DEAH box helicase family protein"
     gene            complement(4000..4113)
                     /locus_tag="PUP22_RS00015"
     CDS             complement(4000..4113)
                     /locus_tag="PUP22_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071588254.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLSPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(4778..4972)
                     /locus_tag="PUP22_RS00020"
                     /old_locus_tag="IAIDMIBB_00004"
     CDS             complement(4778..4972)
                     /locus_tag="PUP22_RS00020"
                     /old_locus_tag="IAIDMIBB_00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041631.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQIRFEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          4613..4698
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          5009..5107
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          5232..5487
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.86"
                     /coverage="89.0"
                     /evalue="1.25e-96"
     Typing          complement(4042..4595)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.075"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cattattaga gccatataca gcagaactta gtgctgctga cattaaatcc tataacaagg
       61 atgattttga tggaaggggt aaatacaaca ctgttagctt gcaaaaaaca agctctccag
      121 ggccgcaaac aacttatgat tatatagata atacaggtaa gaaatggagt tgccctgaaa
      181 aagggtggag aatgacaaag gaaaagttaa aggctttgga aaacgacaat aggctatata
      241 tcactgacaa aacaatacga gaaaaatatt atcttaaaga aagaaaagag atcgggaaac
      301 aaatagataa tttttggcat gacattggga atttaaatag ggtttccgat gttgctttta
      361 ttgatggaca aaaaactgaa aaattaatgc aacggataat tagccttgct tccaaagaaa
      421 atgatatcgt cttggacttt catctcggca gcggaactag cgcagcagca gcccataaaa
      481 tgggacgtcg ctatatcggt attgagcaga tggattacat caatgagatc accgtgccac
      541 gtttgcaaaa agtcattgag ggtgagcagg gaggtatctc aaaagacgtc aactggcagg
      601 ggagcggcag ctttgtctac gttgaactag tggaactgaa cgcttacttc atacatgaaa
      661 tccagaaagc gcagtccacg gaagaactcg aaaaactctt tgccgtgatg aagacagagg
      721 catatctcaa ctatcaggtg gctctggaaa atgtgctgtc cgctgagtac gaagtggatg
      781 ggatcctcca taaggtagca ttcagtgaac tggaactgca tgagcagaag cagcttttga
      841 ttgaaatcct agataagaac cagctctacg tgaatgtctc tgatatggag gacagcgacc
      901 tgaacatttc agagagtgat aaagccttta cccgcagctt ctacggaatg gagtgacggc
      961 atggcgaaga aaccgaagtc agcaggtaga gagaagctgc tgtttaacaa gctgaagcag
     1021 ttcgatgaac caggactgtt ccacgataac tatcagacgc cggactatat tcaggcaaac
     1081 ctgaaagata tcctgcgtcc ctatcagcac agcgcactgc gttacctgca ttacacgcaa
     1141 cgtaaacgtg atgacgcgct tctgcattac cgtcacctgc tgtttcacat ggcaaccggt
     1201 gcagggaaaa cgatggttat ggcaggaacg atactctatc tatttaaaga gttcgggtat
     1261 cagaacttca tctttttcgt acataccgac gcgattatcc agaaaacgcg agagaaccta
     1321 cttaacccgc aatcgccgaa gtacctgttc agtcatgagc tggaaatcga cggggaaaaa
     1381 atcaccatcg caccggtaga tatattcccg tccgtaccgg aacgcaacac gatttacctc
     1441 aagctctcga ctatccataa gatgcacgat gagctgaaca gctatcgaga gaacagcatt
     1501 acctacgagg atttaaaaga aatcccgctt gtgctactcg gtgacgaggc gcatcacttc
     1561 aacgccggca ccaaagctag aggcaagtcg aaaacctcac cggagaacga ggagcagact
     1621 tgggagcgta cgattgagaa catcctcgac ctgcgaccgg ataaccgcct gttcgagttc
     1681 accgctacca ttgaccttgc aaacaaagat atcggacaga aataccgtga caaggtggta
     1741 tatcagtatg atctgaagca gttcatgtca gacggttact cgaaaaaggt catgctgttg
     1801 gaagcaaacc agaacgacag cgacaagatg ctcgatgcgg tactgttaag ccagtaccgt
     1861 aaactgacgg cagcagatca cggcatcacc ggctttaaac cggtcattct gttcaagtcg
     1921 aataagattg cgatatcaaa ggcgaagcag gaggagtttt cgcaactgat tgccgccatg
     1981 acacctgaga gcatcagacg tcatctggct aacaaaaaac tccagctcag ctctgacacc
     2041 agtatctggc ataaagtgat tcagcgttac gctgacagtg atttagtgac ggtgactgga
     2101 caaattcagg aggactttaa cgactttaac ctgctgaacg tcaacaagtc agacctgctg
     2161 gaggaaaacc ccgtcctgct gaatacactg gaagaagtcg ataatccagt gagagccgtc
     2221 tttgctgtcg cgaaggtgaa tgaaggctgg gacgtactta acctctacga tattgttcgc
     2281 ataagcgagc aggcgagcag cagcaaaacc agcacggaca gcgaagcgca gcttattggg
     2341 cgaggtgcgc gttactaccc gttcatttat gacggtcaac gtagcttcac ccgccgtttt
     2401 gataacagta cgaaggattt gagcgtactg gagcaactgc actatcacac catcaatgaa
     2461 ccggcgtaca tcaaaacact gcacgcctct ctggagcagg cggacattga cgtgcatcag
     2521 gatggcggag gtacggttga acacgccaga ttgaaagaag actttaaaaa gtccgccgtg
     2581 tatcaggcgg gtaagctgta ctttaacgaa gtggaagaga ttgagagaag cagccgaaac
     2641 tgggaaacct actcgctgga aacacgcttt gaaatacagt atcagatcgc ttgcgaggag
     2701 tcgctggaca acctgacagg cacaaagggg gggatcataa aaccggaacc actggtactg
     2761 gacgagcgtt tttaccgcaa ggcgatgcaa agaatcagct tttacgcgct agataacctg
     2821 aaacgcttct tcccgaaact gacaggtatt cgggagttca tacgcagtga cgcctatctt
     2881 ggtaagctga aaatcaccgt cagcgtaccg cagtcactgg atttcaccac cgtaccggca
     2941 aaagataagc tgcacctact ggaaaacgtt ttgctgcgta tctcagagaa tgtgcgtcgc
     3001 aatgaccaga aggtaaaagg cacgtaccga tttatcagcc agccggtgaa agaggtgatt
     3061 aaagattaca gtttgcatat tgacccgtcg gtagccatta accagaaaat caccgccgct
     3121 cccaccattg gtaaaaaatg gtacgtgtac gataatgcga tactaaatca gcttgagcat
     3181 cgtctgataa aaatgctgga agcattcatg ccgaagctaa aagcgcgtta tgacgatatt
     3241 tacgtactgc gtaacgacga gcagtcaacg cgctttaagc tcacggagtt cggcggcgtg
     3301 cgtgggttta tgccagattt catcatgata ctgacgcgcc attcagacaa cacttactgg
     3361 caggtgttcc ttgaaccgaa gggggatgac cgtctgctgg atgacgcatg gaaagaacgg
     3421 atgctcgaaa cactgaatga cagggagcgc attgtaattg atgaaaatga gcacgtcaga
     3481 ctggttggga ttaaattctt tgccaatagc cagatggacg tttttgtcag cgacatgcag
     3541 aacaagctga atgatggaga atcgctcgaa acctcctcgc tctccctacc actgtaagtc
     3601 acgacaataa aataacggag cattacgctc cgttatttta ttgttatctc gaactacctc
     3661 ccagggaggt gatgattgct cctgtaatgg ttgcattgtt tgttcttgct gttgcgaatc
     3721 attagttttg aatggtggtt aaaaaccgtt cgctatgttt atgcctgggg ggggggatga
     3781 gggggaaaag attcaaggat cctcttgtga tccttttttt ctgcgcgtaa tctgcagcct
     3841 gtaaacgaaa aaaccaccct ggcaggtggt ttttcgaagg ttagctaatc ctggcagatt
     3901 atctaaccgg gtaactggct tggaggagcg cagtcaccaa aatctgttct ttcagtttag
     3961 ccttaacagg tgcataactt caagacaaac tcctctaaat cagttaccag tggctgctgc
     4021 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4081 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4141 caccgaaccg agataccaac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4201 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4261 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccgac tctgacttga
     4321 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcggcgc
     4381 ggcctttttc cggttcctgc tcttttgctc acatgttctt tccggcctta tcccctgatt
     4441 cggtggataa tctaattact gtctttgcgt gagttgatac cgctcgccgc agccgaacga
     4501 ccgagcgtag cgagtcagtg agcgaggaag cggaagagcg tcggaagttg cattttctcc
     4561 ttacgcatct gtgcggcatt tcacatcggt tatggtgtac ttttcataca atctgcactg
     4621 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     4681 gccccgacac ccgctaaaac cccctgacgc gccctgacgg gcttgtctgc tgacgaaaac
     4741 gcccacggga aagccgtgac cgcctccgtc agcttcgtta tgttcctgtt tcactttcgg
     4801 cctcaaagcg aatctggatg ctgttctgga gttcttctgc gagttcgtgc agtcgttcgc
     4861 acatggccgc ctgttcgtcg gcatccagag catccagttt ttcaagcagt gtcaggctct
     4921 ggcttttgat aaatcgcgcc atattcagcg cagtttgttg ttgtttgttc accttccgtt
     4981 gttctccgtc agttccgggt gtcgggggga ccctgagcag gtggtaattg taagacgttg
     5041 cgggcgacgg tattacaatt gcacatcctg tcctgtttct ttaggacgta tcatggcaca
     5101 gcatatcaac tgataaataa tccctgtgca gtgcagccgc tgttgcataa aatttcgctc
     5161 tgcgtcgagt gtttcttaat caataattcc gctgatactc cggaaaaacg cgagtctgtt
     5221 ctcatgcctg aaatgcccac accccacgca aaaacaagtt tttgctgatt tttctttata
     5281 aatagagagt tatgacaaat tagttcttct tgctctcttt gtgatattta aaaaagcggt
     5341 gttggcgcag ctacaacacc gcgccgacac cgctttttag gggtggtact gactattttc
     5401 ataaaaaaca tcatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
     5461 acgcccctgg gggcgctgcc aggggtgtct gttcagatat gtagcaatga gtcagggatt
     5521 cacgaactga aaatgtccga aaatgtgtct catgggtact gtatgcgccc aaaccgatcg
     5581 tcattcactt tcatgcatag ctatgcagtg agctgaaagt gatcctgacg catttttccg
     5641 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
     5701 ttttagcggt gcgcgcaaga gcgtttatgt taaatactac tcgttctggt gcattttccc
     5761 gcaactgaat ccaactcatc gctgttgggt tccagcgccg aacgctatac cttacaatca
     5821 ttccaatatt taacagtcat aaggccatga catgcagacc gaattaataa aagaacttaa
     5881 caaggtttta aaagcgttcc cgcaattctg gaatggagag gaattacaac gttcaatggt
     5941 gagtgacgct atcagtcaaa agcagccaga tcttatcaag gcattagtcg ataatgaaaa
     6001 agtccgctct gtttatggtg tggatattga tggcattttt atctttgatt ttgacaaatt
     6061 atgtggtctt ctaaaataca aagaatattg ggctaatagc tttactaaat accggaacaa
     6121 agtgggatta actagcgaag gtaaatacct tgattacagt tctgatgtgg tgctggattt
     6181 tcctttcaag gattgtattc ttgaaggtgg aatgaccaaa gaaaatcagg gaaaagatga
     6241 gatttattat aatgaaatca tagcaatgga tgaagttgac tctttgcttt ctcccaaggc
     6301 gttcactaat gctaagcgat ataccaaaga gggggtaaaa gaaaatatca ctaaatttaa
     6361 cgatgatgaa aatttaataa ttaagggaaa caatctcatt gcactacatt caataaaaaa
     6421 actttacgca ggaaaagtaa agttaattta tatagacccg ccttacaata cagggagtga
     6481 tagctttaag tataatgatc gattcaatcg ctcaacatgg ttgacattca tgaaaaatag
     6541 gcttgaggtg gctaaagaaa tgctttccga tgatggcttt atttttattc aatgcgatga
     6601 taatgaatca gcatatctta aagttttatg tgattcaatt tttggagaag agaattatag
     6661 aaactcaatc tattggcata gaacctacgc agggaaaact gtaaaaagta agttgccatg
     6721 gaatacagat acaatattgt tttattctaa aaaagaaagt t
//
