LOCUS       NZ_OP242314             3649 bp    DNA     circular CON 24-MAR-2024
DEFINITION  Escherichia coli strain LA170 plasmid pLAO76, complete sequence.
ACCESSION   NZ_OP242314
VERSION     NZ_OP242314.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363357
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600985.1-RS_2024_03_24
            Annotation Date              :: 03/24/2024 01:16:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 4
            CDSs (total)                 :: 4
            Genes (coding)               :: 4
            CDSs (with protein)          :: 4
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242314.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA170"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO76"
     gene            join(3615..3649,1..193)
                     /locus_tag="PUP24_RS00005"
     CDS             join(3615..3649,1..193)
                     /locus_tag="PUP24_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_273503371.1"
                     /translation="MLHLTTTNSDNVMLRKSEIILIKSCGKSKTKIFLRGVEEGIMVDA
                     SFEDIKFAINNNLHDLLGIQAETTELKLAK"
                     /name="hypothetical protein"
     gene            complement(970..1392)
                     /locus_tag="PUP24_RS00010"
                     /old_locus_tag="EDALDFBA_00002"
     CDS             complement(970..1392)
                     /locus_tag="PUP24_RS00010"
                     /old_locus_tag="EDALDFBA_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_097311099.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKCIKVID
                     TSELIRVFGSLDGVIDAVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1392..1583)
                     /locus_tag="PUP24_RS00015"
                     /old_locus_tag="EDALDFBA_00003"
     CDS             complement(1392..1583)
                     /locus_tag="PUP24_RS00015"
                     /old_locus_tag="EDALDFBA_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_096245219.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGK"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2064..2213)
                     /locus_tag="PUP24_RS00020"
                     /old_locus_tag="EDALDFBA_00004"
     CDS             complement(2064..2213)
                     /locus_tag="PUP24_RS00020"
                     /old_locus_tag="EDALDFBA_00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162854494.1"
                     /translation="MPSEYDEQRDSTDTQLPGEYDLFDLMAKVTEENLHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     Typing          complement(2207..2418)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.783"
                     /coverage="100.0"
                     /evalue="2.05e-63"
     Typing          2398..2875
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.583"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 catgttacga aaaagtgaaa ttatcttaat aaaatcatgt ggtaaatcaa aaacaaaaat
       61 ttttttacga ggtgtggagg agggaataat ggttgatgca agttttgaag atataaaatt
      121 tgctatcaac aataacctcc acgatcttct cgggatccaa gccgaaacca cagaactaaa
      181 actagcaaag tgatacagca cctgaagcat gaaggttcga agtaaagcaa catattaaac
      241 ataacatccg ttatcagcac cgcctgagag gctctcaggg cgtttctgac gggaaaccgg
      301 caaaacaggc aacaaaccac ccgaaaaccc gccagaaacg cgcaggctct gtttttacgg
      361 catgcatgag tatgcgcaca agtgcatagc gcggaaattg acaccattca gccagggtaa
      421 gtcctgtttc cggcgttctt cccatccgaa aacggtgaac ggggacgcaa tttattgcgt
      481 ggccgtgaag cgttgccggc gcagccggaa aaccggtttt cagcacgttc aggaatcggg
      541 ttcgtcgacg gccggcaatc acgttatggc aagtcgccgg acgactcacc acacgattca
      601 ccggcgattc gactcgtgac ttgactctag agtcaccacg gtcaaaaaaa cgccaaagcg
      661 acgattcgac tctagagtca agtcgtcata agttactgat agtgtggagt gagtcgtcga
      721 tgactctatg acgagtcgtc ggcaagtcgt acacgaatca ccgacgactt ggattgacct
      781 tacagaaggt aatactgcgt aatttaataa caaccaactg actgatatta atgagttatc
      841 tgcaaaaatt taattttgcg tggggtgtgg atatttttag tgtgaggagc gttcagagat
      901 ctggcgattt ttgggaaggt tcacgaacgc cccggcatgg ccggagagga aaactcaagc
      961 atggcgggat caggattttt tccagaattt ccaccacggg gaatggggtg gtgaagaagg
     1021 ttcgtgcttg tgctccagaa gctgcatagc ctgacgaagg gaatcaatat gctcatctcg
     1081 agccttaaca gcccctttaa gaccatcgtt ttcagcttca agtaaagcga ttctatgttg
     1141 taaatcatgt aaaccaacgc tgttaatacc tgtagattct gcgtcacttt ttacatcata
     1201 ttttacagca tcaattacac catcaagaga accaaacact ctaataagct ctgatgtatc
     1261 aataacttta atacacttcc cgtgctttac aacagaggtg taacttaact taccactgtt
     1321 aatcatcttg taaatagtag ggcgactaac cttaacaagc cgtgctgctt cactaataga
     1381 cactctggcc atcacttacc atcctgtaat gcatccagtc tcgaggacag tgtacggaaa
     1441 agatgctccg catcatcgtg gagtttctca caaaggtcgg cctcagcatc gagatcaagc
     1501 tcgttaagct tctccagtag gagcagagac tgattctgga tgaatttagc catgttcagc
     1561 gctgttttct gttgtttcgt cataaggaac cttagagcat caatgctcag tgtaaaaccg
     1621 gtaagggggt cggatgtacg aatggttcag agaacggatc tgaggtaatc ctgtagttat
     1681 tttgtagttt atctgtagtc atcgcactaa aaatcaggat taaggcatca gcttttgtag
     1741 tctttctgta gtttacctgt agtccggaaa aataatccgt tcatgcgagc atgaacgagg
     1801 tcgttaatga cgtttttttg tcaccgacgc accttaacct ccgcagatca cccgccatga
     1861 ttgattattt tttaacctca aaatggtcat acacctaacg cagccgcgat tttcatcgca
     1921 actgcatgaa ttacgtagag aaaatgcaat gcaccgttta ttacggtaat acgcgagtat
     1981 taaaatcatc tgtaatcaca tgaaaataaa gaaataagat gtaataccgg cgtattacca
     2041 cgtacgcggg caagcgaaaa aagctataac gcttcccggc cgacaggcgg cccagaatcc
     2101 acaggggcat gcaggttctc ttcagtaacc ttcgccatta aatcaaaaag gtcatattcg
     2161 ccgggaagct gcgtatcagt cgaatcccgt tgttcatcat attcagatgg catggggtga
     2221 tatccagtag ctgaggggtg ttgcgggtgt cggggcgcag ccctgaacca gtcacgtagc
     2281 gctagcggag tgtatactgg cttaatatgt tggcatgagt gcgattatca ggaaagtgct
     2341 tcatgtggca gggaaaaatg tggctcaggg cgtaagtgca aaatacccgt gacgatgaaa
     2401 tgacgcctcc tcgctcactc ggtcgctacg ctcctgccgt tcgactgcgg cgggcgttgc
     2461 ctgcacacgg ataagagcaa aggcatacgg catgacaagc ggggcagaaa aaggatgcgg
     2521 cgctgccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa
     2581 atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct
     2641 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc
     2701 cgcgtttatt tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg
     2761 gactgtatgc acgaaccccc cgttcagtcc gactgctgcg ccttatccgg taactatcaa
     2821 cttgagtcca acccggaaag acacgataaa gcgccactgg cagcagccac tggtagacag
     2881 gatgtgcgag agatgtatcg agagttcttg aagtggtgcc ctgactacgg ctacactgaa
     2941 aggacaaatt ggtgacatgt ctcgcacaag actgttacca aggttagaga atctgccagg
     3001 attaactaac cttcgaaaaa ccacctgcca gggtggtttt ttcgtttctt ggataagaga
     3061 ctacgacgac aaatgatgga tctcaagaag atccttttaa agtccaaaaa tttccgttct
     3121 ccacactacc caccatgagc acttatcaat cataaaaatc atgataggcc actgaatcgg
     3181 caagggttta acagacacat cagagtcatt taagatgact taacgctcca atagacggtg
     3241 taagttaact gtcgctgccg cgctttacgg cagtggttgc agacaagcag agggggcata
     3301 cccacaactc atgcaccttt ttttgttttt cctgtatgtg gggtttgacg ctcaacgtaa
     3361 aggtttcaca cgttaagggg tttttgtccg ataaatcaga atattgtgga cagtgaaaaa
     3421 tgtgcctaaa gcggtgtgtt tttaatcgtc aaatagcact taacggacat catttgtcca
     3481 ttaaccgatt tttcagacga cttttctcgc ctaacgtgag ttttcgttcg cctgagcatc
     3541 agccacctat gtacaaaaaa tataactagg aataatctca ataatcttca tcaagatacg
     3601 caaaaaaaac gataatgctt catctgacga caacaaacag cgacaatgt
//
