LOCUS       NZ_OP242313            33826 bp    DNA     circular CON 24-MAR-2024
DEFINITION  Escherichia coli strain LA170 plasmid pLAO74, complete sequence.
ACCESSION   NZ_OP242313
VERSION     NZ_OP242313.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363357
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33826)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 33826)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Sequencing Technology             :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028600975.1-RS_2024_03_24
            Annotation Date                   :: 03/24/2024 01:28:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 45
            CDSs (total)                      :: 45
            Genes (coding)                    :: 44
            CDSs (with protein)               :: 44
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242313.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..33826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA170"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO74"
     gene            complement(join(32954..33826,1..135))
                     /locus_tag="PUP24_RS00005"
                     /old_locus_tag="MMNELFJG_00047"
     CDS             complement(join(32954..33826,1..135))
                     /locus_tag="PUP24_RS00005"
                     /old_locus_tag="MMNELFJG_00047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243325.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_000243324.1"
                     /translation="MVLKNNKNSDCNDVQSLLAQGNQLLEGAYDITLIEMRLLYLALTK
                     IDSRKPQPANEYTLFAKEYRDTFSLDSKNCYEQLKSAASSLGSKPIVTYEWNEAKKRID
                     AVKRFWFSSIRYGVGNSESDITLRFSDSVSQYLYELKSEFTQMNLEHMVKLDTPFSFRL
                     YSWLYKYKNLNRNKKHSGVISTDPITIEWMKERTGLTGKYPVYKDFKKRVLDPAVDIIN
                     ANTNLSVTYEGIKSGRKIESVVFTYLVENETHDGKKIATKPLRPRLPSRPRVIKGSSAE
                     VAWAKKCIDVMKNYLISLREYDDKLKLSRPDLQKVKLWHEITGWDFEESFWFSD"
                     /name="replication initiation protein"
     gene            complement(1148..1348)
                     /locus_tag="PUP24_RS00010"
     CDS             complement(1148..1348)
                     /locus_tag="PUP24_RS00010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001328427.1"
                     /translation="MVFLMVVSNEAAESDSGLLCDTLGRGIAGSVDLCIVEMKWSHKSI
                     GKRIYHVDIKGILLKHEKILG"
                     /name="hypothetical protein"
     gene            1679..2014
                     /locus_tag="PUP24_RS00015"
                     /old_locus_tag="MMNELFJG_00002"
     CDS             1679..2014
                     /locus_tag="PUP24_RS00015"
                     /old_locus_tag="MMNELFJG_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026069.1"
                     /GO_function="GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001147119.1"
                     /translation="MPYTAKRYQTENGEVPYTDWMKKLRRKDQTAALKVDSRIARAMGG
                     NFGDHKFERDGVWELRVDYGPGYRVYYSIEDGEIILLLIGGNKKTQTADLDKAVSYLQD
                     FKKRSKK"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2011..2361
                     /locus_tag="PUP24_RS00020"
                     /old_locus_tag="MMNELFJG_00003"
     CDS             2011..2361
                     /locus_tag="PUP24_RS00020"
                     /old_locus_tag="MMNELFJG_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000127483.1"
                     /translation="MTATKNVKSQKNDYSASVDHNAAMIEELRADPDYANAYLANALEE
                     INEPGGLGGFLVALRQVIEARGGISEAARKSGLARQSIYRALSPNGNPTITTLAQLTSV
                     AGLQFTLSKSSH"
                     /name="hypothetical protein"
     gene            3000..3515
                     /locus_tag="PUP24_RS00025"
                     /old_locus_tag="MMNELFJG_00004"
     CDS             3000..3515
                     /locus_tag="PUP24_RS00025"
                     /old_locus_tag="MMNELFJG_00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025396.1"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025396.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVETRV"
                     /name="J domain-containing protein"
     gene            3620..4162
                     /locus_tag="PUP24_RS00030"
                     /old_locus_tag="MMNELFJG_00005"
     CDS             3620..4162
                     /locus_tag="PUP24_RS00030"
                     /old_locus_tag="MMNELFJG_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001083908.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKNY
                     SVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVLQA
                     IEYEKHQVEKKDEIMILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDLQIR
                     GLSIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     gene            4159..4410
                     /locus_tag="PUP24_RS00035"
                     /old_locus_tag="MMNELFJG_00006"
     CDS             4159..4410
                     /locus_tag="PUP24_RS00035"
                     /old_locus_tag="MMNELFJG_00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001230708.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDA
                     VLNENELQHIDFTCAGLRKDDETPVKNLIKEFMLNETR"
                     /name="hypothetical protein"
     gene            4397..4624
                     /locus_tag="PUP24_RS00040"
                     /old_locus_tag="MMNELFJG_00007"
     CDS             4397..4624
                     /locus_tag="PUP24_RS00040"
                     /old_locus_tag="MMNELFJG_00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000801440.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQN
                     SQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
                     /name="hypothetical protein"
     gene            4974..5315
                     /locus_tag="PUP24_RS00045"
                     /old_locus_tag="MMNELFJG_00008"
     CDS             4974..5315
                     /locus_tag="PUP24_RS00045"
                     /old_locus_tag="MMNELFJG_00008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001328548.1"
                     /translation="MAPLTIAQQGRRFKMKCSSVFTSTTNHVFTFERVTLCTIILMHKD
                     TGQQYVVLFTDNNKIRDYKTGIVPQFGELKQSDVDLVLFYRDEYEKYFDSLKDGDECLS
                     FKDFIECLR"
                     /name="hypothetical protein"
     gene            5407..5772
                     /locus_tag="PUP24_RS00050"
                     /old_locus_tag="MMNELFJG_00009"
     CDS             5407..5772
                     /locus_tag="PUP24_RS00050"
                     /old_locus_tag="MMNELFJG_00009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000854262.1"
                     /translation="MKTLKQAAMQFASDLRNNRCFKAAYNDALLELDREDLNAIVQCTF
                     LFEPKRTLAELDKLIKKIEVEEDYEFCVSLDKGNGLHEVRTFKSEREALDAYRILTLYG
                     YTVTIEKKEGNDWLFKV"
                     /name="hypothetical protein"
     gene            <6023..6220
                     /locus_tag="PUP24_RS00055"
                     /old_locus_tag="MMNELFJG_00011"
                     /pseudo=""
     CDS             <6023..6220
                     /locus_tag="PUP24_RS00055"
                     /old_locus_tag="MMNELFJG_00011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /name="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            6583..7086
                     /gene="ddp1"
                     /locus_tag="PUP24_RS00060"
                     /old_locus_tag="MMNELFJG_00012"
     CDS             6583..7086
                     /gene="ddp1"
                     /locus_tag="PUP24_RS00060"
                     /old_locus_tag="MMNELFJG_00012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 1"
                     /protein_id="WP_000781818.1"
                     /translation="MKMIAFRLSDDELKLAEHNAISSGFTSINTFARHNVLNVDVQPSN
                     IPVSNESSKAVKTCLYPHEIELVKRNAALHGMSMSREIAIRVRQSLLKNEVCLYPDEVK
                     ELKKLSTAVDRVGRNIHFIIKGERFCTVNDPDFRKDVVEVIELCKQIDSKLETLTKSVV
                     NRFG"
                     /name="ddp1"
     gene            7090..8319
                     /gene="taxC"
                     /locus_tag="PUP24_RS00065"
                     /old_locus_tag="MMNELFJG_00013"
     CDS             7090..8319
                     /gene="taxC"
                     /locus_tag="PUP24_RS00065"
                     /old_locus_tag="MMNELFJG_00013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase/nickase TaxC"
                     /protein_id="WP_000539545.1"
                     /translation="MGVYVEQEYRIKRAKGTGRDPKSPKLSGRHIHASKSAFNHKVRHG
                     TDKKAYTWTPTKEVTFKITGSGKTAAGIKNGIDYITRNGELEAYCYDGKGSEQTGKGED
                     FNREFTSTLSKGNDYSRTYRGENIDHVKNMVFSPPPEAGVSREDALKATIEFLKETYPN
                     HAFVAVYHDDKEDHPHVHVNIKLRDEETGRRLRLTKSETRKFRNGFHRKLKGMGYDVTA
                     TWKKDPERKREIERLQAENPKRLRNVYKVVDFGETSYQNKAGEKRTPFLTYETLKGGKQ
                     VTIWGKDLKNHFESEKLQPGTLIKVKKLAPTLVRSPMFNDDGTVAGYRETHRNNWQIEN
                     IALERNRERQVHERETRQPEESDVKKQLSRKHEQGHNIGFALEHGFTKDSEEHKKLRIQ
                     QERNWKGLGF"
                     /name="taxC"
     gene            8396..8689
                     /locus_tag="PUP24_RS00070"
                     /old_locus_tag="MMNELFJG_00014"
     CDS             8396..8689
                     /locus_tag="PUP24_RS00070"
                     /old_locus_tag="MMNELFJG_00014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134733.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO:0016987 - sigma factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_000134733.1"
                     /translation="MTEHDAICISALHQIFSDEEHLSEQQKDIILMYAYGYTLNEIADF
                     KGLKPSTVRKYLDSVRAELGGVSLAGIRTLVLIRTNALLVSSLSRISERGNL"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            8686..8901
                     /locus_tag="PUP24_RS00075"
                     /old_locus_tag="MMNELFJG_00015"
     CDS             8686..8901
                     /locus_tag="PUP24_RS00075"
                     /old_locus_tag="MMNELFJG_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000760375.1"
                     /translation="MKKYLLFALPFFVVGCSEEVKSVDWWGQHLTEAKQKQAECEKSGS
                     DSQNCKNVKQALFIQSQKDAPVPTFD"
                     /name="EexN family lipoprotein"
     gene            9078..9254
                     /locus_tag="PUP24_RS00080"
                     /old_locus_tag="MMNELFJG_00016"
     CDS             9078..9254
                     /locus_tag="PUP24_RS00080"
                     /old_locus_tag="MMNELFJG_00016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000660496.1"
                     /translation="MKEKPDKSSEKGVYVTIKLDPRLNAFIEEKTKTSLLSKRKQIIYL
                     LMQSMRIDGYMER"
                     /name="hypothetical protein"
     gene            9257..9877
                     /locus_tag="PUP24_RS00085"
                     /old_locus_tag="MMNELFJG_00017"
     CDS             9257..9877
                     /locus_tag="PUP24_RS00085"
                     /old_locus_tag="MMNELFJG_00017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_001299949.1"
                     /translation="MASLFPALLLCAASVHPDTINDIARVESGFNPYAVAEIIPERERG
                     SYGKSVISHMPKSKEEALTVIREIENRKRRYSVGLMQITNSNFSFYSTSAEKLLDPCEN
                     LSVFEKIIVDCYKRGRSLENALSCYYTGNFSNGKRKEKEFNNTSYVERIGYTGNEKKYV
                     VPGTRSNGGEQRKNRSHNASVIWPETILKSAFVDNSHPTKVIN"
                     /name="lytic transglycosylase domain-containing protein"
     gene            9891..10217
                     /locus_tag="PUP24_RS00090"
                     /old_locus_tag="MMNELFJG_00018"
     CDS             9891..10217
                     /locus_tag="PUP24_RS00090"
                     /old_locus_tag="MMNELFJG_00018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000008647.1"
                     /translation="MSFKKSVCNRISSSVTAVMQRKNKVVMAVAGATVASPAFADGFSK
                     AETLLEKVKTGLSGLSLVTVTIACLWVGYKVLFGGSTIRECSPIIIGAIVIASAAEVAS
                     MMVN"
                     /name="TrbC/VirB2 family protein"
     gene            10236..12983
                     /locus_tag="PUP24_RS00095"
                     /old_locus_tag="MMNELFJG_00019"
     CDS             10236..12983
                     /locus_tag="PUP24_RS00095"
                     /old_locus_tag="MMNELFJG_00019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="WP_000105985.1"
                     /translation="MSTLYKAMTRPAMYVGVPVVPLTVVAGALFLAGVYISKLIWLAIP
                     VAVFVLRMITKQDDHIFNLYFLKLKMLGNSVCNRFFGARAFLSGQYEAVEIDEFVNAMK
                     LNERITTGKYIPYSSHVDKNIVKTKNGDYVATWQLMGINFESISAEMLETIDSQVATLV
                     RSFSGLPVSFYNHSCRASFYDAFTTKSGNKYADIISDCYYGSMKKNKFKGNTLYFTLIY
                     RPDGRVEKLEKRKKSIKEKKDDINIHVKRMNEMINTFSGALDKFTCKLLGMYEENGKVF
                     SSQLSFYNYLLTGKLQKIRVTDSPVYNVLGGVDVFFNHDTGQICRIDGNKFFRSIEIKD
                     FCSETASGVFDVLQYSDADYIITHSYTSMSKSEALSTIKRAEKQLKSTEDDAVTQLQEL
                     EKAKNDIVSGDISFGYYHFTLMVMADSIRELDESVSKITADFTDLGIIPALSTMSLPAA
                     YFAQLPAVFHLRPRLSPVSNVNFVELASFHNFYQGKRDKNCWTEAVAILKTPSKQAYYL
                     NLHNSVLFKDETGEKNLANTKVIGTAGSGKTMFLSYLACSLQKYNNPETFADSAKNKKL
                     TCVFLDKDRGAELCIRMLGGEYYTVKSGEPTGWNPFALEATKRNRIFVKQLMEILCTRN
                     GERLSTRERLLISESVDAVMDFPPGEMREYGITRMLEHLMQRDDRDEQENGIILRLSQW
                     ANGQAHGWVFDNAKDTFNIQHVNNFGIDGTEFLDDPMVCAPITFYLLYRITQLLDGRRL
                     VIFLDEFWKWLQDEAFSDFVYNKLKTIRKLNGLVIPATQSPDEILKNKISRAVVEVCST
                     SIYLANPDADYNDYVEGLKLTPEEFNIVKNLDPMSRQFLIKKSSLKKGDGKSFSALATL
                     DLSGLGGYLKILSASADNLEIFESIYHEGMEPDDWVPEYLERAI"
                     /name="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
     gene            12995..13711
                     /locus_tag="PUP24_RS00100"
                     /old_locus_tag="MMNELFJG_00020"
     CDS             12995..13711
                     /locus_tag="PUP24_RS00100"
                     /old_locus_tag="MMNELFJG_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000832280.1"
                     /translation="MKRVKTFMLISLLTASFYSSAGIPVAIDASPEWAVEAARWTERLK
                     QWSETAQHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELTNIYKQGNSYISDFTS
                     NPEGALSSQAKNLFSKYGAFDMCNTGYERNDNLCKARIVSTAASIEQGNEINKQLSNAM
                     SQIQSLSSRIEASKDIKESQDLANALQAQSLKMQAIKMQYDVWNNKNKADHEMLITQEQ
                     EAFIKQQKEAEPLTFD"
                     /name="type IV secretion system protein"
     gene            13723..14817
                     /locus_tag="PUP24_RS00105"
                     /old_locus_tag="MMNELFJG_00021"
     CDS             13723..14817
                     /locus_tag="PUP24_RS00105"
                     /old_locus_tag="MMNELFJG_00021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677777.1"
                     /GO_process="GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000046889.1"
                     /translation="MAQGFFVKYNSTVMDSVDKISSSYQTQFANDIMSLATVSVTLYVL
                     WKGYQILASKTQTPLQDLVWDLSKFAIIIMFITNADGYLTAATDALQGMKDGFSGGVSV
                     WQTLDNLWKSTQNLGAEIYSLDKSTYVKDQGVVGQFLIWTGSLILMAVSVVVFLTADVT
                     MKLLIITAPIFIFCLMFGFIRSMFNNWLQSLFSSILTVLFASLVIRIAMDFQGMILSHA
                     IRAAQTGNVNLVSTGAMGFMAGFLGALLVLIAKGFAVQLAGAGVEGAVQGAAMMGLGAA
                     GMATGKSLMLGGRAGLGFGLGMAGRTGMNSLSGKAGNLMGRGARTAAEWAGEKGYQALA
                     PTGALGMKARRLASLEKARARNAA"
                     /name="type IV secretion system protein"
     gene            15332..16018
                     /locus_tag="PUP24_RS00110"
                     /old_locus_tag="MMNELFJG_00023"
     CDS             15332..16018
                     /locus_tag="PUP24_RS00110"
                     /old_locus_tag="MMNELFJG_00023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_001290765.1"
                     /translation="MSEKIEKKIETAKSFERQLYAENERSKKNAWRVAVAASLLSLCLG
                     AAICVLVPLKEKELAIVAVGEKTGRTELITQVKQEKILQSEALGRYFVNTYITLREGYN
                     YPSLQYDYETVQLYSSNTVKDDYLRLYNSDMAPDKIYHNNGSYVAVEVISNIISDATAP
                     DKLASVRFKKTTRNFTTGQVSVSYWTARVTYRFEPEKSVKSSSRELNPLGFTVTSYQTD
                     REVRGE"
                     /name="type IV secretion system protein"
     gene            16015..16923
                     /gene="virB9"
                     /locus_tag="PUP24_RS00115"
                     /old_locus_tag="MMNELFJG_00024"
     CDS             16015..16923
                     /gene="virB9"
                     /locus_tag="PUP24_RS00115"
                     /old_locus_tag="MMNELFJG_00024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_001299943.1"
                     /translation="MMKKLFIATCLLLPGLVYSAATPLPSGFDARMQTVSYNGANTTVI
                     RSKTGFLTSVVFDEGEAVISAKAGFPAGWEITTDDNVVYINPRPVVQEQEGDEGEKLKK
                     VFQPTEKEWDTNLFVRTTKRIYSLDLILLSEEKQAQPAYVVQFRYPSEIAKKNAEEVRL
                     AKEKQEKLRQKKLISESFEKADAPKNWDYFMRVNEKYDSRRIAPDFAYDNGIFTFLGFN
                     SGKVFPAPFAVRDGQEQTLAFNVETKGKYKIMVIHNVNDKFVLRYGNSVVGVVNKSFGK
                     VLTDQRNTSSPAVERVEVNND"
                     /name="virB9"
     gene            16916..18034
                     /gene="virB10"
                     /locus_tag="PUP24_RS00120"
                     /old_locus_tag="MMNELFJG_00025"
     CDS             16916..18034
                     /gene="virB10"
                     /locus_tag="PUP24_RS00120"
                     /old_locus_tag="MMNELFJG_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_000131271.1"
                     /translation="MTDMKKTDDLVDDANQSKLESKPVHDIGDIRNKNNRMRSGFLFGL
                     MAVLVIGIFALKTYKNYFADDQQKASESTGDTSISQVSKIRTGLGQNFDPVENKAIINP
                     GATGSGNTVSDGHKEVPQEGFRKYLSIPVAGQGGSQTGASGSSRTSQTSEPQEERKESK
                     ENVPGKSGMKVTAINLDPDLYIEENRLIPCALTTRFVSDVAGRISCVFTEDVWSANHHT
                     KLLEQGTKAFGRYQTGTLNHGQGRMFVMWEQLRTPDNKRIDMVNTAAAGPLGEAGIDGW
                     IDSHFWERFGGSLMLSMVQDVAAAAADNAPGKDRNVDYTENSRQAMAEMAKVALENSIN
                     IPPTMYKNQGDIITIMVGEDIDFSDIYELKVK"
                     /name="virB10"
     gene            18036..19064
                     /gene="virB11"
                     /locus_tag="PUP24_RS00125"
                     /old_locus_tag="MMNELFJG_00026"
     CDS             18036..19064
                     /gene="virB11"
                     /locus_tag="PUP24_RS00125"
                     /old_locus_tag="MMNELFJG_00026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401678.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_000017224.1"
                     /translation="MSGVIPVSISNKSLDFYKNQVFQSFLDIEGLTEIAVNRPGEIWTE
                     INGDWTFHNNDGVTFDFCKRFSQTLASFRGDEIGDTKPLLSATLESGERVQVVFPPACE
                     RNTISITIRKPSTRQITHKEYVANGFYDYVQSGKKHRTYDDELLDLFRARNIAAFMELA
                     VAAGKTIVFAGATGSGKTTYMKSLIDFIPLNTRLITIEDAEEIKFFIHKNYVHLFYPSE
                     SGSDSGSIITAAKLIKSCLRMKPDRILLAEIKGGDAWDFVKVAGSGHSGSMTSIHAASA
                     KDAIIQMVTKCYQNRECQNLPFDVLRKIIMDSIDIVVHVGRDGVVRHMSDIYYKGAECE
                     NF"
                     /name="virB11"
     gene            19051..20913
                     /gene="taxB"
                     /locus_tag="PUP24_RS00130"
                     /old_locus_tag="MMNELFJG_00027"
     CDS             19051..20913
                     /gene="taxB"
                     /locus_tag="PUP24_RS00130"
                     /old_locus_tag="MMNELFJG_00027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxB"
                     /protein_id="WP_000847103.1"
                     /translation="MKTFNKSQSVFIFAVMLVAAWFAGSFIFFVLYFSQIKNLKPLKAV
                     FRSIDAYRMDIFTDSLTLSVVTTDIRVLAFVALGAGFVVSLIIPVAALIKLNEKKDNIF
                     GDARFATTNDIRESNSFTLDGDEKDGIIVGIKDKKIIRYVGAAFSAMGAGTRAGKGAGI
                     VITNLMKYWWSVIILDPKRECFNITSLIRKVILGHEVYKFDPFSPVTHRFNPLYYVSMG
                     TSEGFNQLENLALIIYPYKTDGADAGSYLNKTAGGVFKSYAVALWFMIKNDKAGLKTLD
                     IEPVFSMSKINQLFERAEPEHLLSFMNDIRSELRGKDKTLADIGVAGLKAFIEMEDKTK
                     AQLKSNFLNGLSPFANPNVANATDGNDFDLRQVRKKRMTIYFCISGDNARLAEKITNIF
                     FQLAIQVNLEKMPTDDPEIKHDCLFLLDEFPSIGAVDYIKSKSGLIAGYKLKLLIVYQV
                     GSQLEEIYSYAGSKTLLASAPCKIVYSASDVKDARELSEAMGTRTVTIGSKSKSRSRGG
                     TSRSESESLIERPLVTTNELLTLKFSEEILAMKGENPIRCEKAFYYLHEYFFGDFVKVA
                     PELANIFPRKKGKLVMPPQKVFENEIVAKGYLAVKEVPDLDKAA"
                     /name="taxB"
     gene            20922..21344
                     /locus_tag="PUP24_RS00135"
                     /old_locus_tag="MMNELFJG_00028"
     CDS             20922..21344
                     /locus_tag="PUP24_RS00135"
                     /old_locus_tag="MMNELFJG_00028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_001328552.1"
                     /translation="MMKKLVFLLISILAGCSSPPEPTPVQFEKANEVINPSLPYVPDFH
                     GVIKSDVSGKGWVYEITSLSGVQARTPTFYYALAHADRIVVTTHDAGLWFRIRDLLKME
                     GATAVVEWRNEKSFLPEQGKIVFIKAQNEVKNDWKK"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            21328..21633
                     /locus_tag="PUP24_RS00140"
                     /old_locus_tag="MMNELFJG_00029"
     CDS             21328..21633
                     /locus_tag="PUP24_RS00140"
                     /old_locus_tag="MMNELFJG_00029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_000147045.1"
                     /translation="MTGKNRVVLPALLALGLISTNANASDPCASVLCLYGKAVGQGGGS
                     ECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVKG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            21688..21936
                     /locus_tag="PUP24_RS00145"
                     /old_locus_tag="MMNELFJG_00030"
     CDS             21688..21936
                     /locus_tag="PUP24_RS00145"
                     /old_locus_tag="MMNELFJG_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001290044.1"
                     /translation="MSEIRFVTLESDFNNIKEIYSDDELNRLLEAVNQNCPTRIVLGSV
                     EKGKETPLKWCFRTIPERISIEEIDEIKFIQNPSEQG"
                     /name="hypothetical protein"
     gene            22028..22336
                     /locus_tag="PUP24_RS00150"
                     /old_locus_tag="MMNELFJG_00031"
     CDS             22028..22336
                     /locus_tag="PUP24_RS00150"
                     /old_locus_tag="MMNELFJG_00031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000647577.1"
                     /translation="MKARSVRVAGIEIIDSVKTVAYVFTEALLFMVWFSAIVYGLYRVV
                     IVLWDDPLKPQSLMEFLNFIVELYSYVLPSLAVIAVALIAVLSICKILSAIWEGRKQ"
                     /name="hypothetical protein"
     gene            22333..22827
                     /locus_tag="PUP24_RS00155"
                     /old_locus_tag="MMNELFJG_00032"
     CDS             22333..22827
                     /locus_tag="PUP24_RS00155"
                     /old_locus_tag="MMNELFJG_00032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="WP_001215681.1"
                     /translation="MRHFLKALSFVVSLLPATLCAAEIQGKVIRVLDGDTIEVKTLPAK
                     IVVYEVPIRVRLINIDAPEKKQAFGHWSANQLKALLAGQSVTVSYTQTDRYGRIIGRVF
                     TTNGTDASRFMVQSGAAWVYERYNTDKSLPALQREAQEQKRGLWADSNPVPPWEWRHKQ
                     N"
                     /name="thermonuclease family protein"
     gene            22909..24171
                     /locus_tag="PUP24_RS00160"
                     /old_locus_tag="MMNELFJG_00033"
     CDS             22909..24171
                     /locus_tag="PUP24_RS00160"
                     /old_locus_tag="MMNELFJG_00033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000517489.1"
                     /translation="MGKLTLVEQALRLHKEYYGEDNFGTVVKDCDYYPKVAEIFINRKN
                     RKIYSNLDIEPVASTVVHTGSNPVMAKAGRIKKLMFKDGSGAYRIHLGQNEVVHIIRFI
                     LNSKVRMEYAVGTTKSMSMLHNLCEAGRAKLNRSLPRKGIWKLGYYDGVYYHGKARKEE
                     IENAMRFSVHPKFDELENDFNQFFSDIDFYTRYGQSGMRKVLFTGPPGTGKTTIAKALG
                     AKYQDKYVFVYADDYFKDVCYAAAQKKIPVIIIAEEVDELYRADAGTLSFLDGADTPRN
                     VAGTYVIFSTNYPKRIDPRIRKRPGRIDRIISVGAFRTKAAAACAKMYLPEDVEIDLKE
                     LGAVLDRTTPAEIKEIINIAIGMIRGTKNELSVDVIKNARAFLKGTLDLSVQEADEDIE
                     EREELYKKNGAQPDYLSYLED"
                     /name="AAA family ATPase"
     gene            24175..24747
                     /locus_tag="PUP24_RS00165"
                     /old_locus_tag="MMNELFJG_00034"
     CDS             24175..24747
                     /locus_tag="PUP24_RS00165"
                     /old_locus_tag="MMNELFJG_00034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001199073.1"
                     /translation="MQYALVDALERKFLLDALEFGVLKDWKENPVKELPDIDESVHPFH
                     ICYGGYLLNPGVSDSDISRKIKDQAGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGHQK
                     IVPFAALIKADECIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCERRFRV
                     VSKDNWQHTVSEERAIRCAKRLIQCKG"
                     /name="hypothetical protein"
     gene            24752..26905
                     /locus_tag="PUP24_RS00170"
                     /old_locus_tag="MMNELFJG_00035"
     CDS             24752..26905
                     /locus_tag="PUP24_RS00170"
                     /old_locus_tag="MMNELFJG_00035"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="WP_001235747.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHII
                     ESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHAGD
                     PDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKALARS
                     VADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFYYTMT
                     GVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDDKKTAA
                     PLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQFSEAPQV
                     IDALKSVFPQSLDIDSARKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERNVYLAIAQ
                     HYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGESEDNDDSA
                     FELLCKIRTGETLTTKEVVVNEKKTTPPPLFTEASLLAALVRVADFVTDPTIKKLLKDK
                     DKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDALPGIAVNPDM
                     TALWSEKQTAIENGDLTVEEFINELYGELTGMISDVDLGEMKIEPAAPAGQFQRLDSPC
                     PSCGKHIVIRPKGYFCTGCEFKIWSEFSGKKITQAQAEKLVKSGKTDLIKGFKKKSGGT
                     YDTVLVLEDKKTGKLGFPVRAKK"
                     /name="type IA DNA topoisomerase"
     gene            26902..27108
                     /gene="hha"
                     /locus_tag="PUP24_RS00175"
                     /old_locus_tag="MMNELFJG_00036"
     CDS             26902..27108
                     /gene="hha"
                     /locus_tag="PUP24_RS00175"
                     /old_locus_tag="MMNELFJG_00036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="WP_000850859.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAADH
                     RRAELVMNKLYDKVPSGVWKYVH"
                     /name="hha"
     gene            27124..27588
                     /locus_tag="PUP24_RS00180"
                     /old_locus_tag="MMNELFJG_00037"
     CDS             27124..27588
                     /locus_tag="PUP24_RS00180"
                     /old_locus_tag="MMNELFJG_00037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293133.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0030527 - structural constituent of chromatin [Evidence
                     IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="WP_001293133.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNVV
                     IEERREDAEREAAERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGGVR
                     KAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIDKLQPGQVE"
                     /name="H-NS family nucleoid-associated regulatory protein"
     gene            27692..28348
                     /locus_tag="PUP24_RS00185"
                     /old_locus_tag="MMNELFJG_00038"
     CDS             27692..28348
                     /locus_tag="PUP24_RS00185"
                     /old_locus_tag="MMNELFJG_00038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001478769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000972911.1"
                     /translation="MMIKSKKVVVIFVALSSFLLAGCDSPKDANKQNFEKAINTRLEKE
                     CITISPLGMFDSKPYPVQIAVAQANRYTSQERADEINSRQFSALDVLVKADLLTVKNTL
                     VDDVIGFTKTGKKVPGREYALTDEGKKYLKSPERPDFCVGHYKVDEIVDFTEPGDAMGM
                     KITQVNYTFSPTSIAEWAKRDDVRAAFLGLESDLKEKQTKHITLVLKNDGWSAER"
                     /name="hypothetical protein"
     gene            28431..28820
                     /locus_tag="PUP24_RS00190"
     CDS             28431..28820
                     /locus_tag="PUP24_RS00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236115859.1"
                     /translation="MTLSKILTDIFRTANNLTHCKTTNQLLAGDASSTLASAILSCDRY
                     SIFEQDYPRSSLIRQAVRHTGHSVQTSLVTTFKYSVPLSGALFRHMCPYPGLFRITASF
                     SPCPVVHIHLDKNALSEKMSKFFFW"
                     /name="hypothetical protein"
     gene            28905..29729
                     /locus_tag="PUP24_RS00195"
                     /old_locus_tag="MMNELFJG_00039"
     CDS             28905..29729
                     /locus_tag="PUP24_RS00195"
                     /old_locus_tag="MMNELFJG_00039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487124.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_016238959.1"
                     /translation="MSDLPASIFQISRSDAWNLTISPDAARNMRDLASQIGAGMPKYLI
                     APEVSLLLSYIDDLNIRMYFETLWNTGARLNEALALKPADFMLTPTRQYPQPVVILKTL
                     KQRLKEAERRPGRPTTRHPPLHPDPRYRKPPEPAVRLVPVLDAGYALRMREYLATWKKR
                     IKHQPVWDITSRQTPLNWINTAVARAERDGVTFSVPVTPHTFRHSYAMHLTMSGVPPRV
                     LQSLLGHRYARSTEIYTRVFSLDVLTGSGLSFTCDPQLAKQLLGYPERLNNE"
                     /name="tyrosine-type recombinase/integrase"
     gene            29834..30493
                     /locus_tag="PUP24_RS00200"
                     /old_locus_tag="MMNELFJG_00040"
     CDS             29834..30493
                     /locus_tag="PUP24_RS00200"
                     /old_locus_tag="MMNELFJG_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000516230.1"
                     /translation="MGKIITVAGHKGGIGKSTVLCSLCVCVIKKGKTACFLETDSQGSI
                     KDFIEDRKTNARLSEIPYFECYTDIPAMAQKLAARFDYVFVDTPGMKSPAFVKALSCAH
                     ILFTFIEPGSGIEINTLGRLVFDIKTAQAGVNPSMKAWIVLNKCSTNPSDSEASELRRQ
                     LNDDPDWLPVPRQRIYMRTAHKKAYNNGMGVHEYDDKRGNKARGEIELLLKETDIL"
                     /name="division plane positioning ATPase MipZ"
     gene            30659..30961
                     /locus_tag="PUP24_RS00205"
                     /old_locus_tag="MMNELFJG_00041"
     CDS             30659..30961
                     /locus_tag="PUP24_RS00205"
                     /old_locus_tag="MMNELFJG_00041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001062964.1"
                     /translation="MAKIPKKIDQEIKPLDLRQFVSSAAQKPASASAVTRINLTMTDDD
                     LDTAQVFQNKYGASRAEVIRAALAALRLVSAEERRRLFDDIRKNSPKAGRPTVNK"
                     /name="hypothetical protein"
     gene            30998..31249
                     /locus_tag="PUP24_RS00210"
                     /old_locus_tag="MMNELFJG_00042"
     CDS             30998..31249
                     /locus_tag="PUP24_RS00210"
                     /old_locus_tag="MMNELFJG_00042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001482241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000905709.1"
                     /translation="MLIKIIKSVFNFYMLCVQPFIGLVLAGYGAWKFSGDSISALLEPA
                     SYFVIGTVVLFVWYIAWQKERSKEEEFKSSIKPEDFVK"
                     /name="hypothetical protein"
     gene            31299..31514
                     /locus_tag="PUP24_RS00230"
                     /old_locus_tag="MMNELFJG_00043"
     CDS             31299..31514
                     /locus_tag="PUP24_RS00230"
                     /old_locus_tag="MMNELFJG_00043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000399460.1"
                     /translation="MEHCIFDLGIINYHPVITWTVPVAFFFLVLLAGIVFNIGRKSNLI
                     VFILAMLLPVTTVFIFMLLGGKIDMC"
                     /name="hypothetical protein"
     gene            complement(31821..32348)
                     /locus_tag="PUP24_RS00220"
                     /old_locus_tag="MMNELFJG_00045"
     CDS             complement(31821..32348)
                     /locus_tag="PUP24_RS00220"
                     /old_locus_tag="MMNELFJG_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="WP_000577047.1"
                     /translation="MIFNRNKKRDEQQNSSVVLPSTENVFSDFIKNNPALTTNAFTAIG
                     EVDCFTKVPLLSNKESEFLGILSRNIIPDYSVFPKIRLIEFVRPHGSEEAVKELIREVQ
                     NITCDFVLKSYDETVLAVIQFGETATVRQQKKKLIIQRVCAMTEISFFEFKNTVDMMGS
                     EDFCQFLRDDEE"
                     /name="DUF2726 domain-containing protein"
     gene            complement(32484..32888)
                     /locus_tag="PUP24_RS00225"
                     /old_locus_tag="MMNELFJG_00046"
     CDS             complement(32484..32888)
                     /locus_tag="PUP24_RS00225"
                     /old_locus_tag="MMNELFJG_00046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000654313.1"
                     /translation="MKDNIVDFQRIRRDLGAHSTDVANDAVYSDKKKTSRFFYLLTPVR
                     YLLAASLYLVVVIPLGILAGFRFLLQFLGGIAVMGLTIAWYMGADVSGYIVLAAWIALT
                     VCGCAEQIFEWWIESRFAFRLFGIGRTPEE"
                     /name="hypothetical protein"
     Typing          complement(32955..33826)
                     /name="IncX4"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.771"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7091..8316
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          13724..14793
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.72"
                     /coverage="100.0"
                     /evalue="6.640000000000001e-173"
     Typing          15333..16015
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.4399999999999998e-137"
     Typing          16016..16920
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          19052..20910
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.839"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttagtcaat gccagataca gaagacgcat ttcaattagc gttatatcgt atgcaccttc
       61 cagcaattga tttccctgtg ccaacaaact ttgaacatca ttacagtcgc tatttttatt
      121 attctttaag accatacagc tccccttcct gtgagttaaa aatcaaaaga agtttctcac
      181 acccaaatta gggacgcaat ttttttttgt ccctatatag ctaataaacc ataaatctgt
      241 cccctgtttg aagggtcaaa cctggctgaa cggcgcattt tcaccctcag tcagaggcag
      301 tcaatacagc aaaccaggag ggaatgcatc atcaaaaacc catcgagcca aacatcctaa
      361 atgtgatcta agtcactaat ccaattcaga ttcatagaga atccataacg aatccgagtt
      421 gaatctaaaa aaagttatcc acaacctgtg gataaaaaag ttatccacac taaatctgtc
      481 cctagttgac cataaatctg tcccctttta accataaatc tgtcccctga taaaaacgca
      541 aataaccata aatctgtccc cttttaacca taaatctgtc cctagttagc attgcaatca
      601 attgaaaaac aaaagaaaaa acgaccatga aaaaggaaat attatatgta acataggagt
      661 gatctatctt ttagaaaaaa gaaattagaa aaaaagaata gagcgcattg tgaatttttt
      721 gagttggaat cagcaaaatc acaagatgag gtcagaccag agcgtacgga ttcatatgct
      781 gcatcgaaga cattccgctt cctcgctcac taactcgctc cgctcggtcg ttcgactgcg
      841 gcgagcggag tcatttctca catatcgctg gtaattctgt tgttgttcct tcggtgatga
      901 gtcgcttgat agcctataaa acacgttcag aagcgtctca ggcgcacgaa acgggtttaa
      961 atacccctta gggcatggga ggcgcgaaaa acgcttacag aggctttctg ttcatcagaa
     1021 tctgatcctg tgaagaccga gaaagttctc atgtttagtc acaacctgaa tatttcattc
     1081 cgagggcggt ttaatataac tagctgattt tattgaaaga gttcttttaa ttatgatcct
     1141 cattttgcta accgaggatc ttctcatgtt tcaataaaat acctttaata tcaacatgat
     1201 atattctttt gccaattgat ttatgtgacc acttcatttc aacgatacat aaatcaacag
     1261 atccagcaat gcccctcccc aaagtgtcac agagaagccc tgaatcgctc tctgccgctt
     1321 cgttcgatac aaccatgagg aaaaccatgc gaaatgaaaa aaatcgctta aaacgcgata
     1381 cagcgcgttt tagggggtat tcacaaaaca cacgttgcct tgaagaaatt ttgatcaaga
     1441 tttaactact atccggatca tgactacaga tcacagcaac aaggctgcta aaaagcgttg
     1501 agaggcgtta aacgctgtta agactagggc taagtacaat gaaacggaaa aaatgctcag
     1561 aatgcgttac agaaccttcc agaaagctag gatttctcac tttaaagaga catttttctt
     1621 gatctgttgt cacttataag agacaatgta cacaggttaa gggaatgagg gcggcaagat
     1681 gccttacacg gcgaaacgat atcagacaga aaacggtgag gttccgtaca ccgactggat
     1741 gaaaaaatta agacgtaaag accagaccgc agcgttaaag gtggattcca gaattgcccg
     1801 cgctatgggc ggaaattttg gagatcataa attcgaaagg gacggtgtat gggaattacg
     1861 ggttgattac ggtcctggtt acagggttta ttattcaatc gaagatggcg aaataattct
     1921 tctgctcatt ggagggaata aaaaaactca gacagccgat ttagataaag ccgttagtta
     1981 tctgcaagat tttaaaaaga ggtcaaaaaa atgacagcaa ccaaaaatgt gaagtcgcag
     2041 aaaaatgatt attcggcatc cgttgatcac aatgccgcca tgattgaaga attgcgcgca
     2101 gatcctgatt atgcaaacgc ttatcttgct aatgccctgg aagaaataaa cgagcctggt
     2161 ggtttaggtg gttttcttgt tgcgctacgt caggtaatcg aggcgcgagg ggggatttcc
     2221 gaagctgcca ggaaatctgg ccttgcccgt cagagtattt accgcgcatt atcccccaac
     2281 ggtaacccga ccatcacaac gcttgcacaa cttacatcgg tagccggatt acagttcact
     2341 ctcagtaaat ccagccacta atctctccag ttccccctaa gcgggggaat ttccatgcca
     2401 tcaaaaccgc agttatcccg cccgacgttc accggtattt aatcggcaaa tcgtacaatt
     2461 gcgtactggc aatagtttaa tggtgtgtat taatacacat ttaaaggcga tgtggatgcg
     2521 tgtgttaatg ctaatgtatg catacattag cattaacata gagcagattc tggcgcgttg
     2581 cgcccgtagt tcgctgatgc tcacacgcgc agtacagcgc cccgtcctgt tgcgacgcac
     2641 caggacggcg taaactcgct tgcgctcgcg ctaactgcgg cattctgcgg cgcaaatcag
     2701 cataatcatg gcctgctggt cagttaggct ttgcagggtg aagggatggc cctgtgcatc
     2761 ctggtacgtt cgtcagcggc tgcgccgcgc ttcggggcga tgtggctcct gtgctgccct
     2821 ggtattttcg ttcgcccctt ccatccgctt acgcggatta aaaaacgtga taacaatcac
     2881 ttatcgagta aaaaaaacgg gagaatactt gcaaatacga cttaaaggtc gtattattat
     2941 ttcatcgaaa ggggaatggc ccctctcaca atagcccagc agggcaggag atttaaaaaa
     3001 tgaatatcca ggaagcatta aacgtttttg gattatccgg cgaattaact gaaaaagata
     3061 tcaaagcagc atacagaaaa gccgccttaa aatatcatcc agatcgtaac ccgttagggg
     3121 ctgaactgat gaaagcggta aatgcagctt ttgatgtatt gatggcaaat attgataaaa
     3181 taaatcagtt ccagagtact gatgaacatg ctcgatataa ttacggtgat gacctggaaa
     3241 aagtattaaa cgttctttct ggtttatctg gtctggtatt tgaagtgata ggtaactggg
     3301 tctggattag tggagaaact attacacata aggaaacttt aaaagaaatc gggtgtaaat
     3361 gggcggcaaa gaaaaaacaa tggttttatc gtccagacga acataaaagt tactggaatc
     3421 gtgaagaaca cacgatagaa gaaatccgcg caaaatacgg tacaaccgga cagcgcaggg
     3481 cgacagggtg gcaacgcgtg gaaaccagag tctaaccaga acgggggcga gaagcccccg
     3541 atattatagc cagcccgtct tttggggcat cgaacccaaa aagacgggaa caggagccac
     3601 gtagcaaaaa gggaaacaga tgaactcttt ttttgaacaa tatcaccctg tctttgaagt
     3661 tgtctgccgt atcctgggga acggctggcg cgtgaacaaa cttgatgatt gttcgtctcg
     3721 tataaaactg acgtcaccgc agtttaaaaa ttactctgtg catattcgga tggaaaaaga
     3781 ccggttttct gttgtgggga gtgtggatag tcgctcctgg cgtagtccgc atcatgtttg
     3841 cacgttatcc agaaaacgga atcccgttga tattgcagcc gatatcgagc gaaaaattct
     3901 ggtaaatgct tcgcaggagg tgttacaggc gattgagtat gagaaacacc aggtggaaaa
     3961 aaaggacgaa attatgatcc tgaaaggtat gttatcgcaa cttgtccagc ttgaaagctg
     4021 gtatggggca ttgacaggct ttaaagctga aaacggattg aacggtaaag tcaccgaaca
     4081 gggcgacagt tatgatttac agattcgggg cttgagtata gatcaacttg ttaaaattac
     4141 agggtatttg aaacagttat gagaaaaaat aaaggtgatg tgacttattt tcttgaaaaa
     4201 gaaggtgaca attacaggct tacaaaaagg ataaaagcca gaacaaatgt taaaatcggg
     4261 aacaaaacta cgaaaatcac attgtatgat gccgtactga atgagaatga attacaacat
     4321 attgatttta cgtgtgccgg attaaggaaa gacgatgaga cgccagttaa gaatttaatt
     4381 aaggagttta tgttaaatga aacccgataa tacacctgaa aatgtaaaaa aattacgttt
     4441 aaaagcagga ttaacacaaa aggaatgcag tcatatttat ggtgttgggc taagaacctg
     4501 gcagaaaaaa gaggaagtta atactcaaaa cagtcaaagt ctgtcactgg ttgagtttga
     4561 gtttcttttg ttacttgcag gagaacatcc tgaatacgtg ctctgcaaaa gggagtcaaa
     4621 ataaaatgac ctgtgatggt tttatattag tacctggagt tgcgcccgta gctcgctgac
     4681 gctcacacgc gcagtacagc gccccgtcca ggggaaccct ggacggcgta aactcgctgg
     4741 cgcgagcgct aactgcggca ttctgcggcg caaatcagca taatcatggc ctgctggtca
     4801 gttgggcttt gcagggtgaa gggatggcct gtgctgccct ggtattttcg ttcgcccctt
     4861 ccatccgctt acgcggatta aaaacgtgat aacaatcact tatcgagtaa aaaaacggga
     4921 gaacacttgc aaatacgact tgaaagtcgt attattattt catcgaaagg ggaatggccc
     4981 ctctcacaat agcccagcag ggcaggagat ttaaaatgaa atgctcatct gtttttactt
     5041 caaccactaa tcatgttttc acattcgaac gagtaacgct ttgcactatt atccttatgc
     5101 ataaggatac gggacaacag tatgttgtcc tatttactga taacaataaa attcgtgact
     5161 ataaaaccgg aattgttcct cagtttggcg aacttaagca gagtgatgtt gatttagtcc
     5221 ttttctacag ggacgaatat gaaaaatatt ttgattcgtt aaaagatggc gatgaatgct
     5281 tgagttttaa agattttatt gagtgccttc gttaagcatt ataaatgcag gggggaaccc
     5341 tgcattttct ggaaatttca atattgattc tgaaattgtc tattaacatc aatgcaaggg
     5401 aaattcatga aaacgttaaa acaagccgca atgcagtttg catctgatct gcgtaataat
     5461 cgctgtttta aagctgctta taatgatgct ctgttagagc ttgatcggga agatttaaat
     5521 gccattgtac aatgcacatt tctttttgag ccaaaacgaa cattagccga acttgataaa
     5581 ttaattaaaa agatagaagt agaagaagat tatgagtttt gtgtatccct tgataaaggt
     5641 aatggattac acgaagtcag aacatttaaa agtgagcgtg aggcgttgga tgcttatcgc
     5701 attttgaccc tttacggata cacagtaact atagaaaaga aggagggtaa tgactggcta
     5761 ttcaaggtgt gatcaggttg ttccaccaac ctgatcacgt tatcgacaat caaaactgaa
     5821 tgaggttttt aaatgtcaac gcggcagatc gtacagcttg ctgattccct tgtcaaaggc
     5881 tgggaaacca gaattccggc aatgaaattt agtgaatggg atcaattccg gtggtggtta
     5941 aactacctgc aagggtatga aatgttttaa ataaaaaaag gggctttctg cccctttgta
     6001 ttttttgaca aaaatgctat gtgtagatct gtatttacag tcttgtgtgg aggatggaaa
     6061 ggaacctgat accccgttta aaggcgtgtt taacgtcagg cttgatccag aactgcatcg
     6121 tcgggtagca gaaatggcga tggaagaaga cttatcgttg aatgcctttg ttaataaggc
     6181 tctggaaaaa gaagtcagca atcatcgcgc aggggcttaa ttacccttgt tttttatgcc
     6241 ctgtaagggc atgggaggcg ctttgcgccg gaagccccgc agcgcagcga ggacttaaaa
     6301 agcgttacgg cttcagccgt aacaattccc agccctgcca ggcctgtttt ttctttcaca
     6361 aatctatcag ctatacgttt gccagatggt cgaagacgaa agaacctttc agcaacgaaa
     6421 tctgggcata tgcagcgacg attttatagc caggcacagt gccacatatg gtacaatcaa
     6481 tgcaggatag acaatcatgg tcaaaatgtt aacattttat ccatgattgt ccaaaaacga
     6541 tgtttttgcc tgtgcctgcc gcaggctctg ttggggtgag ttatgaagat gatagcgttc
     6601 agacttagtg atgatgagtt aaaacttgca gagcataacg ctatatcaag tgggtttacc
     6661 agcatcaata catttgccag gcataatgta cttaatgttg atgttcaacc atcaaatata
     6721 cccgtcagca atgaatcatc aaaggctgtt aaaacctgtt tataccctca tgaaatagaa
     6781 cttgttaaac gtaatgcagc ccttcacggc atgagcatga gcagagaaat tgctattcgt
     6841 gtgcgtcagt ctttgttaaa gaatgaagtc tgtctttacc ctgatgaagt taaggaactt
     6901 aaaaaattat caactgctgt agatcgtgtt ggtcgcaaca ttcattttat cataaaaggt
     6961 gaaagatttt gcacggttaa cgatcctgat ttcagaaaag acgttgttga agtgattgaa
     7021 ctatgtaagc aaattgattc aaaacttgaa acgttaacca agagtgttgt taacagattt
     7081 gggtgattta tgggtgtgta tgtagaacag gaataccgta ttaaacgcgc taaagggact
     7141 gggcgtgatc cgaaatctcc taagttatcg ggaagacata tacatgccag taaatcggct
     7201 tttaatcata aagtcagaca tggcacagac aagaaagcat atacctggac gccaacaaaa
     7261 gaagtcactt ttaaaatcac aggttcaggg aaaactgccg caggtatcaa aaacggtatt
     7321 gattacatta cccgtaatgg tgaacttgag gcttactgtt atgatggaaa aggttccgag
     7381 caaacaggga aaggagaaga ctttaacagg gagtttactt caactttatc taaaggaaat
     7441 gattactcca gaacatatcg tggagaaaac atcgatcatg tgaaaaacat ggtcttttca
     7501 ccaccaccag aagctggagt aagcagggaa gacgctctga aagctacgat agaatttctt
     7561 aaagagactt atccaaacca tgctttcgtt gccgtatacc atgatgataa agaagatcat
     7621 cctcatgtac atgttaatat taaacttcgt gatgaagaaa caggaaggag attaagactt
     7681 actaagtctg aaaccaggaa gttcagaaac ggttttcata gaaaactgaa aggtatggga
     7741 tatgatgtta ccgctacatg gaaaaaagat cccgaacgta aacgggagat tgagcgatta
     7801 caggctgaaa atccgaaaag actccggaat gtttacaagg ttgtggattt tggggagact
     7861 tcatatcaga ataaggcagg ggaaaaacga acacctttcc tcacttatga aactttaaaa
     7921 ggtggaaagc aggttacgat ttggggtaaa gacctgaaaa atcatttcga gtctgaaaag
     7981 ctccagccag gaacgcttat caaagttaaa aaactggctc ctacgcttgt acgtagcccg
     8041 atgtttaatg atgatggaac tgttgcaggc tacagggaaa cgcatcgcaa taactggcag
     8101 attgaaaata ttgcccttga gcggaacaga gaacgtcagg ttcatgagcg tgaaaccagg
     8161 cagccggaag agagcgacgt taaaaagcag ctatccagaa aacatgagca aggtcacaat
     8221 atcgggtttg ctcttgaaca cgggtttaca aaggattcag aagaacataa aaaactaaga
     8281 atacagcaag agaggaactg gaaaggcctg ggattttaat ttgatttcgg cgcaggtgaa
     8341 caaagcggct actttgctca cctgctaatc acaacaacct acgagggagg ttatcatgac
     8401 tgagcatgat gctatctgca tttctgcact tcatcaaatt tttagtgatg aagaacattt
     8461 gagtgagcaa caaaaagaca ttattctgat gtacgcttat ggctataccc tgaatgaaat
     8521 tgctgatttc aaaggactaa agccttcaac tgtcagaaaa tatcttgatt ctgtaagagc
     8581 tgaactggga ggtgtctcgt tggcagggat cagaacactg gtactcattc ggactaatgc
     8641 ccttctcgtg tcaagtcttt ccaggatcag tgagaggggg aatttatgaa aaaatatctg
     8701 ctttttgctt tgccgttctt cgttgttggt tgtagtgaag aagtaaaaag tgtggattgg
     8761 tgggggcagc atcttacgga agctaaacaa aagcaggcag agtgtgagaa gtctggctct
     8821 gattctcaga actgtaagaa tgttaaacaa gcattgttta ttcagtctca gaaggatgct
     8881 ccagtcccta cattcgatta actaatgttt aaatcatact aaagcgccca tatgggcgct
     8941 tttttttgca tgatcacacg aatcataaca tgaccatttt gtcaatggta tttgatcgta
     9001 ttgttgagca ttgttatttt gcgaatcatg ttcgcatcta aaacaaatcc aaaacacact
     9061 tatagtggat tgattaaatg aaggagaaac cagataagtc atcggaaaaa ggggtttatg
     9121 tcacaataaa actcgatcca aggttgaatg catttatcga agagaaaacg aaaacatctt
     9181 tgcttagtaa gaggaagcaa attatttact tattaatgca gagtatgcgt attgatggat
     9241 atatggagcg ttagctatgg cgtctttatt cccagcattg ttgctgtgtg cggcgtcagt
     9301 tcatccggat acgataaatg atattgcacg ggtcgagagt ggcttcaatc cttatgctgt
     9361 ggctgaaatt attcctgaaa gggaacgcgg tagctatggc aaaagtgtta tcagtcatat
     9421 gcccaaaagt aaggaggaag ctcttacagt tatcagagaa attgaaaaca gaaaaagacg
     9481 ttattctgtc ggattgatgc agattaccaa cagtaatttt tctttttatt ccacgtccgc
     9541 agaaaaactc cttgatccgt gtgaaaactt gtctgttttc gaaaagatta tcgtggattg
     9601 ttataaacga gggaggagcc tggagaatgc gcttagttgt tactatacag gaaattttag
     9661 taacggtaag cgaaaagaaa aagaatttaa taatacaagt tatgttgagc gaattggtta
     9721 tacagggaac gaaaaaaaat atgttgtacc aggtacaaga agcaacgggg gagaacagcg
     9781 aaaaaatcgt tcacacaatg cgtcagttat ctggcccgaa accattctga aatctgcttt
     9841 tgttgataac tcacatccaa caaaagttat taattaacag gacttataaa atgtcattta
     9901 agaaatcagt atgtaatcgt atcagttcat ctgtcactgc tgtaatgcag cgtaaaaata
     9961 aggtagttat ggcagtcgcc ggggcaacag ttgcatcacc ggcctttgcg gatggatttt
    10021 caaaagcgga aacgctgctg gaaaaggtta aaacagggct tagtggtctt tcactggtca
    10081 cggtgactat tgcctgtctg tgggtcggct acaaggttct gtttggtgga agcacgattc
    10141 gtgaatgctc acctatcatc atcggggcaa ttgttatcgc cagtgcggct gaagttgcat
    10201 caatgatggt taactaataa agcggggacg atagtatgtc tacactttat aaagcgatga
    10261 cacgccctgc tatgtatgtg ggtgttcctg tcgtccctct tactgtggtt gcaggggcgt
    10321 tatttctggc gggggtttat atcagcaagc tgatctggct ggctattcct gtcgcggttt
    10381 ttgttctcag aatgataact aaacaggacg accacatttt taacctttac tttcttaagc
    10441 tgaaaatgct cggaaactca gtgtgtaatc gtttttttgg cgcacgagcg ttcctctccg
    10501 gacaatatga agctgttgaa attgatgagt ttgttaatgc catgaaactg aatgagcgaa
    10561 tcactactgg taaatatatc ccatattcaa gccacgttga taaaaacatc gtcaaaacaa
    10621 aaaacggtga ttatgtagcg acatggcaac tgatggggat taactttgag tcaatctctg
    10681 cggagatgtt agaaactatt gactcacagg ttgccactct tgttcgttca ttttccggac
    10741 tgcccgtttc attttataac cattcatgca gggcgagttt ttatgatgcc tttaccacaa
    10801 aatcaggtaa taaatatgct gatataatat cagattgtta ttatggttcg atgaagaaaa
    10861 ataaattcaa ggggaacact ctttacttta cgctgatcta cagaccagac gggcgtgtag
    10921 aaaaactgga aaaacgcaaa aaaagtatta aagaaaagaa agatgatatt aacattcatg
    10981 tgaagagaat gaatgaaatg attaatacat tctctggcgc tcttgataaa tttacctgta
    11041 aacttcttgg tatgtatgag gagaacggaa aggtattttc ttctcaactg tcattctata
    11101 attatcttct gacagggaag ttgcaaaaaa tcagagtaac tgattctccg gtgtataacg
    11161 ttcttggtgg agtggatgtg ttttttaacc atgataccgg acagatatgc cgcattgatg
    11221 gaaataaatt ctttcgttct attgaaatta aagatttctg tagtgagact gcatccggtg
    11281 tttttgatgt gctccagtat tctgatgctg actatattat tacgcattca tatacatcca
    11341 tgagcaaatc agaagcattg agtaccatta aacgtgcgga aaaacagtta aagagtacag
    11401 aagatgatgc ggttactcaa ttgcaggaac tggaaaaggc aaaaaatgat attgtgtccg
    11461 gtgatatatc ctttggttac taccatttca cgctaatggt aatggcggac agtatcaggg
    11521 agcttgacga atctgtaagt aaaattacgg cagactttac tgacctgggc attattccgg
    11581 cattgtcaac catgtcattg ccagcggcat attttgccca gcttccggcg gtttttcatt
    11641 taaggccacg tttatctccg gtatcgaatg taaattttgt cgaactggca tcattccata
    11701 atttctacca ggggaaacgc gataaaaact gctggacaga agcggttgct attctcaaaa
    11761 caccaagcaa acaggcgtat tatctgaatt tacataactc agtactgttt aaagatgaaa
    11821 caggtgaaaa gaatcttgct aatactaaag ttataggaac ggcgggttca ggtaagacaa
    11881 tgtttctgtc atatcttgcc tgttcattac agaaatacaa taaccccgaa acttttgctg
    11941 attctgcaaa aaataagaag ttaacgtgtg tctttcttga taaggacaga ggggctgaat
    12001 tatgtatccg aatgcttggt ggggaatatt acaccgtaaa aagcggtgag ccgacaggat
    12061 ggaatccttt tgctctggag gcaacaaaac gtaaccgtat ttttgtcaag caactgatgg
    12121 agattctgtg taccagaaat ggtgaaagat tatcgacaag agaaagattg cttattagcg
    12181 aaagtgttga tgctgtgatg gattttcctc ctggcgaaat gcgggaatat ggcattacca
    12241 gaatgcttga gcatcttatg cagagagatg atcgggatga gcaggagaac gggattatcc
    12301 ttcggttgtc ccaatgggct aatggtcagg ctcatggctg ggtttttgat aatgctaaag
    12361 acacattcaa tattcagcat gtcaataatt ttggcattga tggcaccgag tttcttgatg
    12421 atccgatggt atgtgcacca attactttct atctgctgta tcgcattacg cagcttcttg
    12481 acggtcggcg tcttgttatc ttcctggatg agttctggaa atggctacag gatgaagcat
    12541 tcagcgactt tgtctataac aagctgaaaa caattcgtaa gctgaacggg ctggttattc
    12601 cggcaactca gtctccggat gaaatactga agaataagat ttcacgggct gtggttgaag
    12661 tatgcagcac cagtatttat ctggctaacc ctgatgccga ttacaatgat tatgtggaag
    12721 ggcttaagct gacaccagaa gaatttaata ttgttaaaaa tcttgatcca atgtcgagac
    12781 agttcctgat taaaaaaagc agtcttaaaa agggggatgg taaatcattt tcagcacttg
    12841 cgacacttga tttatcggga cttggtggat atctgaaaat attgtctgct agtgcagata
    12901 atctggaaat ttttgaaagt atttatcatg aaggaatgga gcctgatgac tgggttcctg
    12961 aatatctcga acgggcaatc tgacaggtga atttatgaag cgtgtaaaaa catttatgct
    13021 gatatcatta ttgacggctt cattttatag tagcgcagga attccggttg ctattgatgc
    13081 cagtccggaa tgggctgttg aagccgcaag atggacagaa cgactcaaac aatggtccga
    13141 aactgcgcaa cattatcaga gtcagattca ggcatataaa gaccagttag ccacggcaac
    13201 aggtataagg aatattgcgg cattcacaaa tgaactaagt aatctacaat cagaactgac
    13261 aaatatttat aagcagggga acagttatat ttctgatttt accagtaatc ccgaaggtgc
    13321 attgagcagc caggctaaaa atctgttcag caagtatgga gccttcgata tgtgtaatac
    13381 gggctatgaa cgtaatgata atctctgtaa agcacggatt gtgtcaacgg ctgccagtat
    13441 tgaacaggga aatgagatca ataagcagtt atcaaatgca atgtctcaga ttcagagttt
    13501 aagtagccga attgaggctt caaaggatat caaagaatcc caggatttgg ctaacgcatt
    13561 acaggcgcag tcgttaaaaa tgcaggcgat aaaaatgcag tatgatgtct ggaataataa
    13621 aaataaagca gatcatgaga tgttgatcac acaagaacaa gaggccttta ttaaacagca
    13681 gaaagaagct gaaccattaa cttttgatta aaaggtggat atatggctca ggggtttttc
    13741 gttaaatata attctaccgt tatggattcg gtagataaaa tcagctccag ttatcaaaca
    13801 caatttgcta atgatattat gtcgttagcg acggtatcgg ttacgttata tgtactatgg
    13861 aaaggatatc aaatacttgc cagtaaaact cagacgccct tgcaggattt agtatgggat
    13921 ctatcaaaat ttgcaatcat aatcatgttt ataactaatg cggatgggta tttaactgcg
    13981 gccactgatg cgttgcaagg catgaaagat ggtttttctg gaggggtgag tgtctggcaa
    14041 actttagata acttatggaa aagtacgcaa aatcttggcg cggaaatata ctctttagat
    14101 aaatcaacat atgttaaaga tcaaggtgta gttggacagt ttttaatatg gactggctcg
    14161 cttattttaa tggctgttag tgtggttgtt tttttaacag cagatgtaac gatgaaactc
    14221 ctgattatta cagcaccgat atttatcttt tgtcttatgt ttggttttat tcggagcatg
    14281 tttaataatt ggttacaatc gttattctcc agtattctga cagttctatt tgccagttta
    14341 gtgattcgga tagcaatgga ttttcaaggg atgatccttt cccatgctat tcgtgctgca
    14401 caaacaggaa atgtaaatct tgtatctaca ggtgctatgg gctttatggc tgggtttttg
    14461 ggggctttat tggttcttat agctaaaggt tttgctgtcc agttagcagg tgcaggtgtt
    14521 gaaggtgctg ttcagggcgc agccatgatg gggctgggtg ctgctggtat ggctactggt
    14581 aagtctctga tgcttggcgg tcgtgctggt ttaggttttg gcttagggat ggccggaaga
    14641 acgggcatga attctttatc tgggaaagca gggaatctga tggggcgtgg tgcgcgaaca
    14701 gccgctgaat gggcgggtga aaagggatat caggcgctgg ctccgacagg tgctttgggg
    14761 atgaaggcga gaagacttgc atccctggaa aaagcacgag cgaggaatgc cgcatgaata
    14821 taatgaaacg ttttttttat gtgatgtccc tgaactggat actgacgttt gttgataaac
    14881 atatcacctc cggatggagc aggaagagaa agctaataac ccagcttatt attttaatca
    14941 cgggaattac cttatttaag gtcttaggtg gaatcagtca gagctggttg tgacatgctt
    15001 cattattttg tcatgatact ttattttgtc ctaatccttc ttttaggagt gtttacctgc
    15061 ttcgcaggaa taagacttgg tttagggctt gcggggaaat ggagaaaggg tatggatctt
    15121 cgctttttgc ttcttattgg tgggattctt tttcttcatc ttggccttta tctcttaaag
    15181 gtgttcgtga acgcttaata aggtgaattt atgcgatgtg tgattctttt gtttttcctg
    15241 tttcagattt ccggatgtac cacaaggaat acccttcctt ctgatgtttc cggcgaactg
    15301 gaacccatta accacagcca ggtaatcagt tatgagtgaa aaaatcgaaa agaagattga
    15361 gacagctaaa tccttcgaaa gacaactcta tgcagaaaat gaacggtcta agaaaaatgc
    15421 atggcgtgtt gcagtggcag catcattact gtccttatgt cttggggctg caatttgtgt
    15481 tcttgtgcca ttaaaagaaa aagagcttgc tattgtggct gttggcgaaa aaacaggccg
    15541 tacagaactg attactcagg ttaaacagga aaagatcctg caatctgaag cgcttgggcg
    15601 gtattttgta aacacgtata ttactttacg tgaaggttat aattatccgt ctcttcagta
    15661 tgattatgag actgtacagc tatatagctc gaatacagta aaggacgatt atcttcgtct
    15721 gtataacagc gatatggctc ctgataaaat ttatcataat aacggttctt acgttgcggt
    15781 tgaggtcatc tccaatatta tttctgacgc aaccgcgcca gataaactgg cctctgtgcg
    15841 ttttaaaaaa acgacgagaa acttcacgac cggacaggtt tcagtttcat actggacggc
    15901 tcgtgtgaca tatcgttttg aaccagaaaa gtcggttaaa agttccagtc gtgagcttaa
    15961 tcctctcggt tttacggtaa ccagttacca gacagatcgg gaagtcaggg gggaatgatg
    16021 aagaaattat ttattgctac atgtttattg cttcctgggc ttgtgtattc agcagcgact
    16081 ccgttacctt ctggatttga tgcaaggatg cagactgtga gttataacgg tgcgaatacg
    16141 accgttatcc ggtcaaagac aggatttctc acatcagttg tttttgatga aggtgaggct
    16201 gttatcagtg caaaggctgg ttttcctgcc ggatgggaaa tcacaaccga tgataatgtt
    16261 gtttacatca atccgcgtcc cgttgttcag gaacaggaag gcgatgaagg cgaaaaactg
    16321 aaaaaggttt tccagccaac agaaaaggaa tgggacacga atctttttgt cagaacaaca
    16381 aagcggattt acagtcttga tctaatcctt ctgtcagaag aaaaacaagc gcaacctgct
    16441 tatgttgtac aattccgcta tccatcagaa atagcgaaaa agaatgcaga agaagtaagg
    16501 ctggcaaaag aaaaacagga aaaactcaga cagaagaaac tgatttctga aagttttgaa
    16561 aaagccgatg cgcctaaaaa ctgggattat ttcatgcgag tcaatgaaaa atatgacagt
    16621 cgtcgtatcg cccctgattt tgcttatgat aatggcatat ttaccttttt aggttttaac
    16681 tcagggaaag tattcccagc tccatttgct gtaagagatg gtcaggaaca gacacttgct
    16741 tttaatgtgg aaacaaaagg caagtataaa atcatggtta tccacaacgt taatgacaaa
    16801 tttgttttac gctatggaaa cagcgttgtt ggtgttgtga ataaatcttt cggaaaagta
    16861 ttaaccgacc agaggaatac atcttctcct gcggtagaac gtgttgaggt gaataatgac
    16921 tgatatgaaa aaaacagatg atttagttga tgatgcgaat cagtcaaagc tggaaagtaa
    16981 acccgttcat gatatcggtg atatcaggaa caaaaataat cgtatgcgtt ctggctttct
    17041 gtttggtctg atggctgtat tagttattgg tatttttgcg ctgaaaacat ataaaaatta
    17101 ttttgcagat gaccagcaga aagcatcaga atcaacaggc gataccagca tttctcaggt
    17161 aagtaaaatc cgtactggtc ttggtcagaa ttttgaccct gttgagaaca aagcaattat
    17221 caatccaggc gctactggtt ctggtaatac tgtttctgac ggtcataaag aagtgccaca
    17281 ggaggggttc aggaaatatt tgtcaatacc tgttgctggt cagggaggga gtcagactgg
    17341 cgcgagtggc agcagcagga catcgcagac atcagagcca caggaagaaa ggaaagaatc
    17401 aaaagaaaat gtgcctggta aatcaggaat gaaagtgact gcgattaatc tcgatcctga
    17461 tctctatatt gaggaaaaca ggcttatacc gtgtgcttta accacacgct ttgtttctga
    17521 tgttgcaggt cgtatcagtt gtgtttttac agaagatgta tggagtgcta atcaccacac
    17581 aaaattactg gaacagggaa ctaaggcttt tggtcgttac cagaccggaa cgctgaatca
    17641 tggacagggc agaatgttcg ttatgtggga gcagttgcgg acgcctgata ataaacgaat
    17701 cgatatggta aatacagcag cggctggtcc cctgggggaa gcaggcattg atggatggat
    17761 cgattctcac ttctgggagc gttttggtgg ttctcttatg ctcagtatgg ttcaggatgt
    17821 agcggcggcg gcggcggata atgcacctgg taaagatcgt aatgtggatt ataccgaaaa
    17881 ctcacgacag gcaatggctg aaatggctaa agttgcactt gagaattcga taaatattcc
    17941 tccaacaatg tataaaaacc aaggtgacat aatcactatt atggtcgggg aagacattga
    18001 tttttctgat atctatgagt taaaggtaaa atagtatgag tggcgttatt cctgtcagca
    18061 ttagcaataa atcgctcgat ttttataaaa atcaagtatt ccagtctttt cttgatattg
    18121 aagggctaac agaaattgct gtcaaccgtc ctggtgaaat ctggacggaa attaatggtg
    18181 actggacatt ccataataat gacggtgtga cctttgattt ttgtaaacgt ttttctcaga
    18241 cgctggcatc atttcgggga gatgaaatcg gcgataccaa gccattactt tcagcaacac
    18301 tggaatcagg agaacgtgta caggtcgttt ttcctcctgc atgtgagaga aatactattt
    18361 caatcactat acgtaaacca tcgacacggc agataacgca taaagaatat gtggcgaacg
    18421 gtttttatga ttatgtccag tcaggaaaaa aacacaggac atacgatgat gagcttcttg
    18481 atttattcag agcgagaaat atcgctgctt ttatggagct tgccgtagca gcagggaaaa
    18541 ccattgtatt tgccggagca acgggttccg gtaaaacaac ctatatgaag agtctcatcg
    18601 atttcatacc attgaatacg cggctgatta cgattgaaga tgcggaggaa atcaaatttt
    18661 ttattcataa aaattatgtg catctttttt acccttcgga atccggcagt gattccggct
    18721 ctatcataac agcagcgaaa ttaatcaaat cctgtttgag aatgaaacct gacagaattt
    18781 tactggcgga aattaaaggt ggcgatgcct gggattttgt gaaagttgct ggttctggtc
    18841 acagtggaag catgacgtcc attcacgcgg cctcggcaaa agatgccatc attcagatgg
    18901 tgacaaaatg ttatcagaac agagaatgtc aaaatcttcc ttttgatgtt cttcgtaaaa
    18961 tcattatgga cagtattgat attgttgttc atgtaggcag ggatggtgtt gtcaggcata
    19021 tgagcgacat ctattacaaa ggggctgaat gtgaaaactt ttaataagtc gcaatctgtt
    19081 tttatattcg ctgttatgct tgttgcggca tggtttgcag gttcatttat tttctttgtg
    19141 ctttacttca gccagataaa gaatctgaaa ccattgaagg ctgtgttcag atccattgat
    19201 gcgtacagga tggatatttt tacagacagc cttactttat ctgttgtgac tacggatatc
    19261 agagttttag cctttgttgc acttggagca ggatttgtcg tgtctcttat tatccctgtt
    19321 gcggcgttga taaaactgaa cgagaagaaa gataatattt ttggtgatgc gcgatttgcg
    19381 acaactaatg atattcgtga gtctaacagt ttcacgcttg acggtgacga aaaagacggg
    19441 attatcgttg gtataaaaga caagaaaatc atacgctatg tgggagcagc attcagtgcg
    19501 atgggagccg gaacaagggc tggtaaaggt gcaggtatag tcatcactaa tcttatgaag
    19561 tactggtgga gtgttattat tctcgatcca aaaagggagt gttttaatat caccagtctt
    19621 atcaggaagg tgattttagg tcatgaggtt tataaatttg atccctttag tccggtaacg
    19681 caccgcttta accctttgta ttatgtcagc atgggaacga gtgaaggttt taatcagctt
    19741 gaaaatcttg cgttgattat ttacccctat aagacagatg gtgcggatgc tggtagctat
    19801 cttaataaga cggcgggggg tgtttttaaa tcttatgctg tcgctttatg gtttatgatt
    19861 aagaatgata aggctggact aaaaacgctt gatattgagc ctgtcttctc aatgtctaaa
    19921 attaatcagc tttttgagcg tgcagaaccc gaacatcttc tttcattcat gaatgatatt
    19981 cgttcagaat taagggggaa agacaaaacg cttgctgata ttggtgtcgc tggccttaag
    20041 gcattcattg agatggagga taaaacaaaa gcacaactca aaagcaactt tcttaatgga
    20101 ttatcaccgt ttgctaaccc caatgtggca aatgctactg atggaaatga ttttgatttg
    20161 cggcaggtgc ggaaaaagcg gatgacaatt tatttctgta tttctggtga taatgccagg
    20221 ttggctgaaa aaataacaaa tattttcttt cagcttgcta tacaggtgaa tcttgaaaag
    20281 atgccaacgg atgatccgga aataaaacat gactgccttt ttctactgga tgaattcccg
    20341 tcaattggtg ctgtagatta catcaagagt aaatctggac tcattgctgg ttacaagctg
    20401 aaattgttga ttgtttacca ggtcggctct cagcttgagg aaatctacag ttatgcaggc
    20461 agtaaaacat tactggcaag tgcaccctgt aaaatcgttt attccgcttc tgatgttaaa
    20521 gatgcgcggg agttatcaga agcgatggga acacgaacag taactatcgg ttcgaaaagc
    20581 aaaagtcgta gtcgtggagg aacatcgaga tctgaatcag aaagccttat tgagcgtcca
    20641 ctggtaacaa caaatgaact acttacactg aaatttagtg aagaaatcct ggcaatgaag
    20701 ggagaaaacc cgattcgttg tgaaaaggca ttctactatc tccatgagta tttctttggt
    20761 gattttgtga aggttgctcc ggaacttgca aacattttcc ccaggaagaa aggaaagttg
    20821 gttatgcctc cacagaaagt atttgaaaat gaaatcgtcg caaaagggta tctggcagta
    20881 aaagaagttc ctgatctcga taaagcggca taaaggagaa catgatgaag aagttagtgt
    20941 ttttattgat ttctatcctt gctggttgtt catcaccgcc agagccaaca cccgtgcagt
    21001 ttgaaaaggc aaatgaagtg atcaatccct cattgccata tgtccccgat tttcacggtg
    21061 tcataaagtc agacgttagc ggtaaaggat gggtttatga gatcacttcc ttatccggag
    21121 tacaggccag aacgccgacg ttttattacg ctctggctca tgcggacagg attgtagtga
    21181 caacacatga tgctgggctg tggtttcgaa tcagagatct gttgaagatg gaaggggcaa
    21241 cagcagtcgt ggagtggcga aacgagaaaa gttttttacc tgaacaggga aaaatagttt
    21301 tcattaaagc acaaaacgag gtgaaaaatg actggaaaaa atagagtggt tttacctgcc
    21361 ttgctggcgt taggattaat atcaacaaat gctaatgcat ctgatccttg cgcttcggta
    21421 ctttgcttat atggaaaagc agtcgggcaa ggtggaggga gtgagtgcag aagtgctgaa
    21481 aaggattttt ttaatattct gaaaaagaaa aaaggaagca ttcgctggag taaaacattt
    21541 gatgcccgta aagcatttct gaatcagtgt tcaacggctg atccggctgc tatctcaaaa
    21601 attatgagta agttcggacg cgttaagggc tgattaaaac ctgatacccg cgccaacggg
    21661 tatcagtgga catacaggag aaaaattatg agcgaaatcc gatttgtaac tctggaatcc
    21721 gactttaaca atattaaaga aatttattct gatgatgagt taaaccgttt gctggaagct
    21781 gttaatcaga attgcccgac cagaattgtg ttgggtagcg tggagaaggg gaaagaaaca
    21841 ccactcaagt ggtgttttag aacaatacca gagcgtattt caattgagga aatcgatgaa
    21901 ataaaattta tccagaatcc ttctgaacag ggataaaggt aaacgccgca gattccagat
    21961 tttaatctgg gatttgcggg gttgaacaac cgaagggcgt tagtttcccg ttatccatga
    22021 ggtatatatg aaagcaagaa gtgtgagagt tgccggaatt gaaattatcg atagtgttaa
    22081 aacggtcgct tatgttttta ctgaggcctt actgtttatg gtctggtttt cggcaattgt
    22141 ctatgggctt tatcgtgttg ttatcgtttt atgggatgat cctttaaagc cgcaatcgct
    22201 catggaattt cttaatttca tagttgagtt gtatagctat gtacttcctt cgttggctgt
    22261 tatcgcagtg gctttaatcg ccgttttatc catttgtaaa atactctcag caatatggga
    22321 gggaagaaag caatgagaca ttttcttaag gctctttcct ttgttgtttc cctgttacca
    22381 gctacattgt gtgcggctga aatccagggg aaagttattc gcgttctcga tggcgacacc
    22441 atagaagtta aaacactacc cgcgaaaatt gttgtgtatg aagttccgat cagggttcga
    22501 ttgataaata tcgatgctcc tgaaaagaaa caggctttcg ggcactggtc agcaaatcag
    22561 cttaaagctc tgctcgcagg acaatcagtt accgtctctt atacgcagac agaccgctac
    22621 ggacgcatta tcggtcgtgt gttcacgacg aacggcacgg atgccagccg ctttatggtt
    22681 cagtctggcg ctgcatgggt atatgaacgt tacaacacag ataaatcatt accggctttg
    22741 caacgggaag cgcaggaaca aaagcgtggt ttatgggctg acagtaatcc ggttccaccg
    22801 tgggaatggc gacataaaca gaactgacgc gcgttgcttg tccaacccct ccactacctt
    22861 aaataaattt caggcagtgt cacggtttta aatatacatg gtgcgcttat gggtaaatta
    22921 actctggtag agcaggcttt acggcttcac aaagaatatt acggtgaaga taattttgga
    22981 actgtcgtaa aggattgtga ttactatcct aaagtggcag agatttttat aaacagaaag
    23041 aaccgtaaaa tttattcgaa tctggatatc gaacctgttg cttcaaccgt tgttcatacg
    23101 ggaagtaacc ctgttatggc taaagctggg agaattaaaa aacttatgtt caaagatggt
    23161 tctggtgcat acaggattca tcttggtcaa aatgaagttg ttcatattat tcgcttcatt
    23221 cttaactcta aagtcaggat ggagtacgcc gttgggacta ctaaaagtat gtcaatgctc
    23281 cacaatcttt gcgaggcagg acgcgctaaa ttaaaccgct cattgccacg taaaggtatc
    23341 tggaaacttg ggtactatga tggtgtttat tatcacggta aggcaagaaa ggaggaaatt
    23401 gaaaatgcta tgcgtttctc agttcatcca aaatttgacg agctggaaaa cgactttaat
    23461 cagttctttt ctgatattga tttctatacc cggtatggtc agtcaggtat gcgtaaagtg
    23521 ctctttactg gcccccctgg aacaggcaag acaactatcg caaaagcttt gggggcaaag
    23581 tatcaggata agtatgtttt tgtttatgcc gatgattact ttaaagatgt ttgctacgct
    23641 gctgctcaga agaaaatacc cgtcattatt atagctgaag aggttgatga actttatcgg
    23701 gctgatgccg gaacgttaag ttttcttgat ggagctgata caccgcgaaa tgtcgcaggt
    23761 acatatgtga tcttttctac aaactatcca aaacgcatcg acccacgtat taggaaacgt
    23821 ccaggtcgaa ttgacaggat aatctctgta ggtgctttca gaaccaaagc tgctgccgct
    23881 tgcgccaaaa tgtacttacc agaagatgta gaaattgacc ttaaagagct tggcgcagtg
    23941 ctggacagaa cgactccggc tgaaatcaag gaaattatta atatcgcaat cggaatgatt
    24001 cgcggaacca aaaatgaatt atcagtggat gtaataaaaa atgctcgtgc ctttttgaaa
    24061 ggaacccttg atctgagcgt gcaggaagca gatgaagata tagaggaaag ggaagagctt
    24121 tataaaaaga acggggcgca acctgactat ttaagttacc tggaggatta acacatgcaa
    24181 tatgcattag ttgatgcgct ggaacgaaaa tttttgctgg atgctcttga atttggtgtt
    24241 ctgaaggact ggaaagaaaa tccggtaaaa gaacttcctg atattgatga atctgttcac
    24301 cccttccata tctgttatgg tggatattta ttaaaccctg gtgtttcaga ttcagatatt
    24361 agcagaaaaa taaaagacca ggcaggattc tggctggcag ctattgatga tacccgcatg
    24421 gattgtcatt caatagctta ttatgatatt cacacactcc ctctgatttc gtgtggtcat
    24481 caaaagatag ttccttttgc cgcgttaata aaagctgatg aatgcatcat ttcaaaaatt
    24541 gcttcgtatt ctggttttgc tgtaacagcc tttttgagaa ttaaagagtg ggatattgca
    24601 actaatatac ttaaccgtga gggaattttt gcctttaatg gctgtgagcg cagattcaga
    24661 gtagtaagta aagataactg gcaacataca gtatcggaag aacgcgctat ccgttgtgcc
    24721 aaaagattaa ttcaatgtaa aggataacaa aatgagactt tttatcgcag aaaaacccgc
    24781 agtagcaaat gatattgtta aggcacttgg tggcaatttt acccgccatg atggctggtt
    24841 tgaaagtgat aacgccattg tgactaactg ttttggtcat attatcgaat cacagccgcc
    24901 ggaaaactat aatcctgaat acaaagcctg gaaggttgaa acgcttcctt tacgtcttta
    24961 tcccgtgaag tatcagcctg ttgaaagtgc agcaaaacag gttaaaacga ttctcgagct
    25021 tatcagacgt ggagacgtga ctgaaattgt tcacgctggc gatcctgatg atgagggaca
    25081 gctacttgtt gatgaagtcc tggaatatgc aggaaacaca aaacccgtaa agcgcgttct
    25141 gattaacgac aacacgcttc cggcagtgaa aaaggcactg gcaaatctta aagataatcg
    25201 tgatttcaaa gggctttacc ttaaggcgct ggcgcgttca gttgctgatg ccgtctatgg
    25261 cttctccatg acgcgtgctt acaccattcc ggcaaaagcc agaggatatc agggtgttct
    25321 gtctgtcggg cgcgtccaga ctcccgttct tggcctgatt gtgaatcgta cccgtgctaa
    25381 ccagaaccat aaatccagtt tttactacac catgaccgga gtctttcagc gtggtgctga
    25441 tgttatcagg gcgaactgga agccagggga atttgctccg ctgaccgacc gtaaattact
    25501 tgataaagcg tgggcagacg gaacggcagc atcccttgca ggaaaaccgg ctacagttga
    25561 agcggcagca actgatgata aaaaaacggc tgcaccgttg ccgtttaacc tggtcagact
    25621 ccagcaatac atgaacaaaa agttcaaaat gacggcacaa aaaacactgg atattacgca
    25681 acaattacgt gaaaaatata aagcgattac ttataaccgc tctgattgct catatctttc
    25741 tgatgaacaa ttcagcgaag cgccgcaggt tatcgatgcc ctgaaatcag tctttcctca
    25801 gtcgctggat attgattctg cacgtaaaag caaagcgttt aacagtgcaa aggtgactgc
    25861 gcatactgca ataatcccga ccgccagtgt gcctgatgtt aacgcactca gcaccgatga
    25921 gcgcaatgtt tacctggcga tcgcacaaca ctatcttgtt cagttcatgc ctgaaaaagc
    25981 ataccaggaa gtatcggttg ccattcagtg tggtgatgag tcgttctatg cccgtgccag
    26041 aaaaacaact gacagcggat ttgaggcgtt tcttggcgcg gaaaccacag acgaaggtga
    26101 atcagaagat aatgatgatt ccgcttttga actgctctgt aaaattcgca caggagaaac
    26161 actgacgaca aaagaagttg tcgttaatga gaagaaaaca acaccgccgc cattatttac
    26221 cgaagcctcc ttgcttgctg cgcttgttcg cgtcgcggat tttgtcactg acccaacgat
    26281 taagaaattg ctgaaagata aggataaaga caaaaaagat gaacatggtg gcattggtac
    26341 gccagctacc cgcgcagcca ttctggaaac actgaagaaa agaaactata tcacgctgga
    26401 aaaagggaaa cttattccta ctgataccgg atatgcgctt attgatgccc tgcctggtat
    26461 agcggttaat cctgatatga cagcattatg gtctgaaaag cagacagcca ttgaaaatgg
    26521 cgacctgacg gttgaagagt ttattaatga gctgtacggt gaactgacag gcatgatttc
    26581 tgatgttgac ctgggcgaga tgaagattga acccgctgcg ccagcagggc agtttcaacg
    26641 cctggactct ccctgccctt cctgtggtaa acatattgtt atcagaccaa aaggttattt
    26701 ctgtaccgga tgtgaattta aaatctggag tgagttttct ggtaagaaaa tcacccaggc
    26761 gcaggcagaa aaactggtta aatcagggaa aaccgatttg attaagggat ttaaaaagaa
    26821 aagcggtgga acgtatgata cagttcttgt tcttgaggat aagaaaacag ggaagctggg
    26881 ttttccggta agggctaaga agtgaaaaca aagcaggaat ggctttttca gttaagaaaa
    26941 tgtacatcaa gagatactct tgaaaaagtt attgagatta accgttacaa actgccttta
    27001 tcagaatcag aggcatttta ttctgccgca gatcatcgcc gcgcagaact ggtgatgaat
    27061 aaactgtatg ataaggttcc ttccggcgtc tggaagtacg tccactaaac aagaggatta
    27121 attatgagcg aactgactaa agaagatgaa tacggcatta tcagccggac tatgatgaat
    27181 attcgttcat tgcgtgtttt tgcccgtgag attgattttg agcagttgct cgaaatgcag
    27241 gaaaagctca acgttgttat tgaagaacgt cgtgaagatg ctgaacgtga agcggctgaa
    27301 cgccaggaac ttgaagccaa acgtcagcag gccattgaat acatcatatc tttaggtctt
    27361 gatcccgaat cactccttgc tcctgtaaca gctgatacag tgaaaactag gcggaaagcc
    27421 aaaggcggag taagaaaggc taaataccgc tttaaggatg aaaatggcga gatcagagaa
    27481 tggtctggta atggcaagcg tccgcttgct ttacagaagc tgcttgatga gggccatttc
    27541 atggaagatt tcctcattga caaactgcaa cctgggcagg ttgaataaaa taatcgtgat
    27601 gccgtcgcaa acaagtttgc gacggcaaat ggaggggaca gaacggccag cggcgctggc
    27661 aagaaatttt atcgtttgat aaagagggaa aatgatgatc aaatcaaaaa aagtagtggt
    27721 tatttttgtt gctttgagtt cattcttgct ggcagggtgt gattcaccaa aagatgctaa
    27781 taagcaaaac tttgaaaaag ctattaatac tcgactagag aaggaatgca ttaccattag
    27841 tccgctggga atgtttgata gtaagcctta tccggttcag atagctgtgg ctcaggctaa
    27901 tcgatacaca tctcaggaac gggcagatga aataaattct cgacagtttt ccgcgctgga
    27961 tgtactggtc aaagccgatt tattgacagt aaaaaatacc ctggttgatg acgttattgg
    28021 ttttactaag acgggtaaaa aagtgccagg tcgtgaatat gctctgacag atgaggggaa
    28081 aaaatatctg aagtctccgg aacgacctga tttttgcgtc ggtcattata aggtggatga
    28141 gatagttgat tttaccgaac ctggtgatgc tatgggaatg aagatcacgc aggtgaatta
    28201 cacattcagt cccaccagta tcgctgaatg ggcgaaacgc gatgatgttc gggctgcatt
    28261 tttaggactt gagtctgatt tgaaagagaa acaaacaaaa catattacac tggttttgaa
    28321 aaatgatgga tggtctgctg aacggtaggc tgtaatggtg ttgctacgtg aaaaataccc
    28381 cacgtatatc ccctgctttt tcacggattt cacgtagcgc accgccgtta atgactctat
    28441 caaaaatact caccgatatt tttcgcactg caaacaacct aactcactgt aaaacaacaa
    28501 atcagcttct cgccggtgac gcatcgagca cgctggccag cgcgatcctc agctgcgacc
    28561 gctacagtat ttttgaacaa gattaccctc gctccagttt aattcgccaa gcagttagac
    28621 atacaggtca ttctgtccag acttcattag ttacaacatt caaatattct gttcctttat
    28681 ccggtgccct gttccggcat atgtgcccat atcctgggtt attcaggata accgcttcct
    28741 tttcgccctg ccctgtcgtt catatccacc tggacaaaaa tgcattatcg gaaaaaatgt
    28801 ccaaattttt tttttggtga tcggaatttt tgttgtcgga cgcacctctg cttcaggtca
    28861 taattgctgg cacgtccaga aaaacaggtg cggagattaa aaaattgagt gacttacccg
    28921 ccagcatttt ccagatttca cgtagcgacg cctggaattt aaccatctct cccgatgcag
    28981 cccgtaatat gcgggatctt gcatcacaga ttggcgcagg aatgccaaaa tatctgatag
    29041 caccggaagt ctcactgctt ctctcctaca tcgatgatct gaacatacgt atgtactttg
    29101 agacgctctg gaataccgga gcacgactga acgaagctct ggcgctgaaa cctgccgatt
    29161 ttatgctgac tccgacgcgg cagtacccgc aacccgtggt gatcctgaaa acactcaagc
    29221 aacgtctaaa agaagcagaa agaagaccag gacgtccgac caccagacat ccaccattac
    29281 atcctgatcc gcgatacaga aaaccaccag agcctgctgt aaggctggtt cctgttctgg
    29341 atgccggata cgcattgcgt atgcgggaat acctggcgac ctggaaaaag cggatcaaac
    29401 atcagcctgt gtgggacata acatcgagac agacaccgct gaactggata aacaccgcag
    29461 tggcacgggc agagcgtgac ggcgtaacat tttcggttcc ggtcactcct cacacgttcc
    29521 gccacagcta cgccatgcac ctgacaatga gtggcgttcc tccgcgcgtc ctgcaatccc
    29581 tgctgggaca ccgttatgca agaagcaccg agatttacac ccgcgttttt tcgctggacg
    29641 tccttacagg aagtggcctg tcctttacct gcgatccaca gcttgcaaaa cagcttcttg
    29701 ggtaccctga gcgccttaat aatgagtaat aatacacacc attaaactat tgccagcacg
    29761 caattgtacg gttatacttt caccacaaaa gattgtgtat taatacacac catttaacca
    29821 ggaggatttt gtgatgggaa aaatcattac agttgcagga cacaaaggcg gcataggaaa
    29881 aagcaccgtg ctttgttcct tatgtgtatg tgtcataaag aaagggaaaa cagcctgttt
    29941 cctggaaaca gactcacagg gaagtatcaa ggatttcatc gaggatcgta aaaccaacgc
    30001 cagactgtca gaaataccgt atttcgaatg ctacacggat attccggcta tggcacaaaa
    30061 gctggccgcc cgctttgatt atgtgtttgt tgatacacca ggcatgaaat ctccggcgtt
    30121 cgttaaagct ctgagttgtg ctcacattct tttcacattc attgagccag gatcaggcat
    30181 tgagatcaac acactgggac ggctggtttt cgacattaaa acagcacagg ccggagtcaa
    30241 tccgtcaatg aaagcatgga ttgtcctgaa taagtgctca actaacccgt cagacagtga
    30301 agcgtcagaa ctgcgccgac aactcaatga tgatccagac tggcttccgg ttccccgcca
    30361 gcgtatctat atgcgtactg cgcataaaaa agcctataac aacggtatgg gcgtacatga
    30421 atacgatgat aaacggggaa acaaagcccg tggcgagatt gagttattgt taaaagaaac
    30481 ggatattctg taatgtgtat tattacacac caaatttgat aatataccca tatccgatgc
    30541 gatatacggc ggggatggct tatctgacca agcctggcaa tttgcagtac gtggatgccg
    30601 gaactttccc tgccataaat gatgtgtata gttacacacc ttataaaaag gataccttat
    30661 ggcaaagatc ccaaagaaaa tagatcagga aattaaacca ctggatttac gccagtttgt
    30721 aagttctgct gcacaaaaac cagcatcggc atccgcagta acgcgtataa atctgactat
    30781 gacagatgat gatctggata cagcgcaggt gttccagaac aagtacggtg caagccgtgc
    30841 tgaagtaatc agagcagcac tggcagcgtt acgtctggta tcagcagaag aacgtcggag
    30901 gctgtttgat gatataagga aaaacagccc gaaagcagga agaccaaccg tgaataaata
    30961 gagtgtttta ttacacacta ttaaagggga taaaaatatg ctcataaaga taattaaatc
    31021 tgtattcaat ttttacatgc tctgtgtgca gccttttatt ggactggttc ttgcgggtta
    31081 tggtgcatgg aaattttcag gtgacagtat ctcagcatta ctggaaccag catcgtactt
    31141 tgtcattgga actgttgttt tgtttgtatg gtatatagca tggcaaaaag aacgttctaa
    31201 agaagaagag tttaaatcaa gtataaaacc agaagatttt gtgaagtgag gatacgtaat
    31261 aataccagtt tataatcaga cattagggca ctcaatctat ggaacactgc atctttgatt
    31321 taggcattat aaactatcat cctgtcatta catggacagt accagttgca tttttctttt
    31381 tagtattgct ggctggtata gtattcaata ttggcaggaa aagtaatcta atcgttttca
    31441 tcctggcaat gttgctccca gtaacaacgg ttttcatttt tatgttactg ggcggtaaga
    31501 tcgatatgtg ctgaccaatt aaaagaggat taaacaatga taaattcaat cgcgtttaat
    31561 cttatagcgt tatgtataat atatatatca tcactattta aaaaaagtaa cttcagaata
    31621 ttttatataa ctgtatctac gtgctcttta ttattatcat gtgtaccaaa caagtacggt
    31681 atgattggta tagcagcatt ggtatttaca actatgttag gaccattatt aattatttta
    31741 gaggataaca aacaataaat tatgacaatg cgtttgttaa gcccgcaaat atgtgggctt
    31801 atttttaatt tctaatattt ttactcttca tcatctcgca ggaactgaca aaagtcttcg
    31861 ctccccatca tatcgacagt atttttaaat tcgaaaaaag aaatttctgt catagcacac
    31921 acacgctgga taatcagttt tttcttttgc tgtctgacgg ttgcagtttc accaaactgg
    31981 ataacagcaa ggactgtttc atcatatgat ttcaggacaa aatcacaggt tatattctgc
    32041 acttcacgga ttaattcttt tacagcttcc tcgctaccat gaggacgaac aaattcaatg
    32101 agtcgtattt tgggaaaaac ggaataatca ggtataatat tacgtgaaag aatgcctaaa
    32161 aactctgatt ccttatttga gagtaagggt actttagtaa aacaatcaac ctccccaatg
    32221 gctgtaaaag cattggttgt caaagctgga ttgttcttaa taaaatcaga gaatacattt
    32281 tcagttgatg gcaaaacaac agaagaattc tgttgttcat ctcgtttttt attacggtta
    32341 aatatcattt catattcctt ttaaaagagc ctgcggcagg cacaggaaaa aacatcgttt
    32401 ttggacaatc atggataaaa tgttaacatt ttgaccatga ttgtctatcc tgcattgttc
    32461 tttactgtct gtaagatcag cgctcattct tccggcgttc tcccgatacc aaaaagacgg
    32521 aacgcaaaac ggctttcgat ccaccattca aaaatctgct ctgcgcaacc acaaacagtt
    32581 aaggcgatcc atgcagcaag aacaatgtaa ccactgacat ctgcacccat gtaccaggcg
    32641 atagttaacc ccatcactgc gataccgcct aaaaactgca acaagaaacg aaatcccgca
    32701 agaatcccca atggaataac aacaaccaga taaagggaag cagccagaag gtatctgaca
    32761 ggtgtcagca gataaaagaa cctggatgtt ttctttttgt cagaataaac ggcatcattt
    32821 gcaacatctg ttgaatgagc gcccaggtct ctcctgatac gttgaaaatc cacgatgtta
    32881 tctttcatat catcctctct tttaagtgag ttataaaaag agaaaaaaca ggtaacgtaa
    32941 acagtagcat tctttaatca ctaaaccaga acgactcttc gaaatcccat ccggttattt
    33001 catgccataa cttaaccttc tgaagatctg gccttgataa ttttaactta tcatcatatt
    33061 cccttaaaga gataagataa tttttcataa catcgataca tttttttgcc caggctacct
    33121 ccgcagagct tcctttaatg acccttggtc ttgatggcaa tcgagggcgt aacggtttag
    33181 ttgctatttt ttttccatca tgtgtttcat tctctacaag atatgtgaac acaacagact
    33241 cgatctttct gccggactta ataccttcat aggtaacact cagattcgta tttgcattga
    33301 taatatcaac agcaggatca agcacccgtt tcttaaaatc cttatatact ggatatttcc
    33361 ctgtaagccc ggttcgctcc ttcatccatt caatggttat aggatctgtc gagatcactc
    33421 cactatgttt cttatttctg ttcagattct tatatttata caaccatgaa taaagtcgaa
    33481 atgaaaatgg cgtatccagt ttcaccatat gttcaagatt catttgtgta aattcgcttt
    33541 tcagttcata aagatattga ctaaccgaat ctgaaaaacg caaagttatg tcactttcac
    33601 tattcccaac accatatctt attgaagaaa accagaatcg tttgactgca tcgatccttt
    33661 tttttgcctc gttccattca tacgtaacaa ttggctttga gcctaaagaa ctcgcagcag
    33721 attttaactg ttcataacaa tttttcgaat ctaaagaaaa agtgtccctg tattccttcg
    33781 caaacaatgt atattcattt gcaggctgtg gtttacggct atcaat
//
