LOCUS       NZ_OP242312             4067 bp    DNA     circular CON 24-MAR-2024
DEFINITION  Escherichia coli strain LA160 plasmid pLAO72, complete sequence.
ACCESSION   NZ_OP242312
VERSION     NZ_OP242312.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363355
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Sequencing Technology             :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028600965.1-RS_2024_03_24
            Annotation Date                   :: 03/24/2024 01:20:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4
            CDSs (total)                      :: 4
            Genes (coding)                    :: 3
            CDSs (with protein)               :: 3
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 1 of 1
            Pseudo Genes (incomplete)         :: 0 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242312.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA160"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO72"
     gene            745..1863
                     /gene="wzz(fepE)"
                     /locus_tag="PUP32_RS00005"
                     /old_locus_tag="JHEKNMHB_00001"
     CDS             745..1863
                     /gene="wzz(fepE)"
                     /locus_tag="PUP32_RS00005"
                     /old_locus_tag="JHEKNMHB_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243311.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="WP_250110599.1"
                     /translation="MFSMKISKISDEHFSNYNMLPNGNEEIDLFDLIRVLWAAKIKIVG
                     VILFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAF
                     ALFIKKFQSVNSLNEYMRSSPYVMEIIKKKNISALELHRAIVGLSENMKSVDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRNLMEDIRNELEVKTNVEKEIL
                     ALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIATK
                     LNIKKSIKDVSELSGELRNRQYIVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPSNAI
                     IVILSSVIGGMLACGYILLRHAMEARKMSVIE"
                     /name="wzz(fepE)"
     gene            2800..2949
                     /locus_tag="PUP32_RS00010"
     CDS             2800..2949
                     /locus_tag="PUP32_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001515742.1"
                     /translation="MPSEYDEQPDATDALFPGEYDLFDLMAHVTEENLHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            3418..3609
                     /locus_tag="PUP32_RS00015"
                     /old_locus_tag="JHEKNMHB_00002"
     CDS             3418..3609
                     /locus_tag="PUP32_RS00015"
                     /old_locus_tag="JHEKNMHB_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000166329.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCETLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3609..4002
                     /locus_tag="PUP32_RS00020"
                     /old_locus_tag="JHEKNMHB_00003"
                     /pseudo=""
     CDS             3609..4002
                     /locus_tag="PUP32_RS00020"
                     /old_locus_tag="JHEKNMHB_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     Typing          2593..2807
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.091"
                     /coverage="100.0"
                     /evalue="3.48e-51"
     Typing          complement(2084..2612)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.545"
                     /coverage="89.0"
                     /evalue="1.2e-144"
ORIGIN
        1 tcagatctct gaacgctcct caccactaaa aatacccaca ccctacgcaa aattaaaatt
       61 ttgaagataa ctcattaata tcagttagtt gtgggttatt aaattacgca gtattacttt
      121 ctgtaaggtg aatccaagtc gtcggcgatt cgtgtacgac ttgctgacga ctcgtcatag
      181 agtcatcgac gactcactta agactatcag taacttatga tgacttgact ctagagttga
      241 atcgtcattt tggtgttttt ttgaccttta cgactctaga gtcaagtcac gagtcgaatc
      301 gccggtgaat tgtctggtga gtcgtccggc gacttgccat aacgtgattg ccggccgtcg
      361 acgaacccga ttcctgatcg tgctgaaaac cggttttccg gctgcgccgg caacgcttca
      421 cggccacgca ataaattgcg tccccgttca ccgttttcgg ataggaagaa cgccggaaac
      481 aggacttacc ctggctgaat ggcgtgaatt ttcgcgctat gcacttgcgt gcatactcat
      541 gcatgccgta aaaacagagc ctgcgcgttt ctggcgggtt ttcgggtggt ttgttgcctg
      601 ttttaccggt ttcccgtcag aaacgccctg aggccgtttt agtggtgcgt acaattaaaa
      661 tgttatgtta aatgtctgct gaatgatttt taggattttt cctaacatat gatagaataa
      721 aaaaaccata tgttaatata ttgtattttt tcaatgaaaa tctcaaagat atctgacgaa
      781 catttttcta attacaacat gttgccgaat ggtaatgaag aaattgacct ttttgacttg
      841 attagagttt tatgggcagc aaaaataaaa attgttgggg tgattctatt ttttgcttgt
      901 attggctttc ttgtttcttt tgttttgcca caaaaatgga ctagttctgc tgtggttact
      961 cctgctgagt ctgttcaatg gagtgaatta aataaggagc tttccaagct tcatgttctg
     1021 ggagtggatt ttgatatcaa tcgtgatagc gcatttgctc tgtttattaa gaaatttcag
     1081 tccgttaatt cgttgaatga gtatatgaga tcatctcctt atgttatgga gataattaaa
     1141 aagaagaaca ttagcgctct cgaactgcat cgtgcaattg ttgggttgag tgaaaatatg
     1201 aagtcggttg atgataatgc cagtaagaaa aacgacaagt catcattgta tgtatcatgg
     1261 actctgagtt ttactgctcc aacaagtaaa gaagctcacg atgtgttgtc tgggtatatt
     1321 aattatgttt cttcccttgt tgtaaggaat ttgatggaag atataagaaa tgaactagaa
     1381 gttaaaacta atgttgaaaa agaaattctt gcactggatg agataaaaat tagaaaccag
     1441 ctgaatgcag atattcgacg cctcaattat tcactggagg ttgctaatgc ggctggaata
     1501 aaaaaacctg tatacagcaa tggtcagatt atgaaggatg atccagattt tcctgtggct
     1561 ctcggttctg atggtatagc aactaaattg aacatcaaaa aatcaatcaa ggatgtttcg
     1621 gaattgagtg gggagttgcg aaatcgtcaa tatattgtga atcaattggt tgtggcgaaa
     1681 gtgggggatg ttgattttat gccgtttcag tatcaattga gcccaacgtt accggtcaga
     1741 aaggatgggc caagtaatgc cataattgta attctttctt ctgtcattgg agggatgctt
     1801 gcatgtggct atatactttt acgtcatgcc atggaggcca gaaagatgag tgtgattgag
     1861 taaaagttgt gctgatgcat cattttataa gcaactttta taaaaagttc aaaggatctt
     1921 cttgagatca ttcccttaca gccgtagtct tctactctag aaaacgaaaa aaccacctgg
     1981 ggaggtggtt tttcgaaggt tcagtaagtt ggggaacttc tgaaccgtgg taacagtctt
     2041 gtgcgagaca tgtcaccaaa tctgtccttc tagtgtagcc tcagtaaggc tgccacttca
     2101 agaactctca atacatctct cgcacatcct gtttaccagt ggccgctgcc aatggcgtta
     2161 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2221 gctgaacggg gggttcttgc atacagccca gcttggagcg aactgtctaa acggaacggg
     2281 gcgtggtgat ttaggtaaag cttccaccac tacacggatg ccgcaggacg ggaacaggag
     2341 agcgcaagag ggagccacca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2401 gccaccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga
     2461 aaaacggctt tgccgcggcc ttatcgtatc cctgttaagt ttcttcctgg catccaccag
     2521 gaaataaatt tccgccccat tcgtaagcct gcaccgctcg ccgcagccga acgaccgagc
     2581 gcagcgagtc agtgagcgag gaagcggaat atcacctgaa ccagcatctt ctcctgacgc
     2641 cggtaagcct catttcactc ctgccacatg aagcactttg gcaaatcacc acttcggttt
     2701 aaaatattta gccagtatgc actccgctag cgctacgtga ctggttcagg gctgcgcccc
     2761 gaaacccgct aacacccctc agctactgga tatcacacca tgccatctga gtatgatgaa
     2821 caaccggatg cgactgatgc tctgtttccc ggcgaatatg acctttttga cttaatggct
     2881 catgttactg aagagaacct gcacgccccg gtggattctg gcccgcctgt cggccgggaa
     2941 gcgttatagc tttttcgctg gccagcgtac atgtaatacg ctggtattac atgttatttg
     3001 tttattttca tgttgttaca gatgattgta atacgttgcg tattacgcta atacacgaaa
     3061 taactccatt tttttcattt aattcatgca gttgcgataa aaatcgcggc tgcgttaggt
     3121 gtatgaccat ttaaggggca aaaaattatc attctggctg ggtgatctgc gcaggttaag
     3181 gatcatcggc gacgaaaaag cgtctttaac tgcttcgttc atgcaagcat gaacggatta
     3241 tttttccaga ctacaggaag actacaaaag ttgatgattt aatcctgcgt tttagtgcga
     3301 tgactacaga taaactacaa aataactaca ggataacctc agatccgttc tctgaaccat
     3361 tcgtacatcc gaccccctta ccggtttata ctgagcactg atgctctgag ggcccttatg
     3421 acgaaacaac agaaaacagc gctgaacatg gctaaattca tccagaatca gtctctgctc
     3481 ctgctggaga agcttaacga gcttgatctc gatgttgagg cggacctttg tgagacactc
     3541 cacgatgatg cggagcatct tttccgtaca ctgtcctcga gattggatgc attacaggat
     3601 ggtaactgat ggccagagta tctattagtg aggctgcccg actgacgggt aagagtagaa
     3661 caacactgca caggttaatt aaagcaggtg acctgtccac ctgtagcggt gaacgcaatg
     3721 caaaaatggt agatacttca gagcttttac gtgtctttgg tccctttgaa caaccgaagg
     3781 gtgaacacca ttctggacag gtctctgaac agcgtgatac agcctctgct gaccagagtg
     3841 aacaggtgac tcgtcatctg aaacaggaga ttgaacacct tacttttgtc caaggattcg
     3901 cacattgaca gcctgaaaca ggctatgctc cttcttgaaa atcaccaggc aaaattaact
     3961 gaatccccgg cgccatggtg gaaattctgg aaaaaatcct gaccccgtca tgcttcagtt
     4021 ttcctctccg gccatcccgg ggagttcctg aaccttccca aaaatcg
//
