LOCUS       NZ_OP242311             6024 bp    DNA     circular CON 24-MAR-2024
DEFINITION  Escherichia coli strain LA160 plasmid pLAO71, complete sequence.
ACCESSION   NZ_OP242311
VERSION     NZ_OP242311.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363355
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Sequencing Technology             :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028600955.1-RS_2024_03_24
            Annotation Date                   :: 03/24/2024 01:19:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8
            CDSs (total)                      :: 8
            Genes (coding)                    :: 7
            CDSs (with protein)               :: 7
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242311.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA160"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO71"
     gene            join(5441..6024,1..352)
                     /locus_tag="PUP32_RS00005"
     CDS             join(5441..6024,1..352)
                     /locus_tag="PUP32_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_253899394.1"
                     /translation="MATYHLSVKFGGKGKALAHANYITREDKFSQRKDLEHTEHGNMPE
                     WAKDDPAHFWQAADAFERANGSTYREIEIALPRELNEAQRLALVRDFVKQETGGQHTYS
                     FAIHNSQASIDGGEQPHAHIMMSQRVDDGIKRSPEQYFRRYNAKFPARGGARKDSGHNL
                     TPTEQKQALKALRERWERTHNAHMKRYGFEQGFIDSRSLKAQGIDRKPEQHLGFEAAGR
                     LDDSQRQDIKDSRTFSVVDVPEQAKHIDHSDRSWMNASGIEVNRKELVRSLTHDISQQY
                     GREMAARVTKQGNVEIERVKSLSRGFSISR"
                     /name="MobA/MobL family protein"
     gene            355..885
                     /locus_tag="PUP32_RS00010"
                     /old_locus_tag="EBKDGGOF_00001"
     CDS             355..885
                     /locus_tag="PUP32_RS00010"
                     /old_locus_tag="EBKDGGOF_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012634455.1"
                     /translation="MASTEEKLLTLLLSAEHLKEAAKEQQKAVADSLAVARETLKGYRE
                     ISDSVAQQVRDEVRQEVQKMDVAGLIGERISADFQRLEKSVGNMKINADALDKEISIIR
                     SNIIAEYNTLRGYSWKWFAGVAVAFVVLIFATSWFVKTELQDNYNASAAVYQKVADLEK
                     LLTTEQKPEKKKN"
                     /name="hypothetical protein"
     gene            complement(1162..1395)
                     /locus_tag="PUP32_RS00015"
                     /old_locus_tag="EBKDGGOF_00002"
     CDS             complement(1162..1395)
                     /locus_tag="PUP32_RS00015"
                     /old_locus_tag="EBKDGGOF_00002"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="WP_036969363.1"
                     /translation="MMKIDRLVRVTKKENSNGSSSTELEWEPGSKKEILKGAAAGAALG
                     SIVPVIGTGIGAAIGGTLGLIFGKSEEKKDSK"
                     /name="glycine zipper domain-containing protein"
     gene            complement(1429..1650)
                     /locus_tag="PUP32_RS00020"
     CDS             complement(1429..1650)
                     /locus_tag="PUP32_RS00020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_273497498.1"
                     /translation="MMGKGSANIKILHTGIVEGVVDYGSLVICVVNWLVLEKNIEVDSK
                     GAFASIHGPYKQGKDKDTDAWLNKVFSL"
                     /name="hypothetical protein"
     gene            complement(1711..>2001)
                     /locus_tag="PUP32_RS00025"
                     /old_locus_tag="EBKDGGOF_00003"
                     /pseudo=""
     CDS             complement(1711..>2001)
                     /locus_tag="PUP32_RS00025"
                     /old_locus_tag="EBKDGGOF_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004661993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /name="type II restriction endonuclease"
     gene            complement(2086..3657)
                     /locus_tag="PUP32_RS00030"
                     /old_locus_tag="EBKDGGOF_00004"
     CDS             complement(2086..3657)
                     /locus_tag="PUP32_RS00030"
                     /old_locus_tag="EBKDGGOF_00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017782005.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_039026336.1"
                     /translation="MTHIACHINTLQLPFVACTVPLTFTLPAAGCTLIGRNGIGKSLLL
                     ELLAGQRRADSGKIEWLTPISWLSQFAPAQDHDCAASVADLIGWGEPLRALERIEQGSC
                     AQDDFELLADRWNLRSDLADLLAEAGLRLDPHVPVSTLSGGQLTRLRLLALFARPAHFL
                     LLDEPDNHLDASGIDWLSQQLARHRGGYLLVSHDRRLLNQTSHILELSEQGLQSARLSF
                     DEFLAQQAEVQAARSTSLAQAVRSEKAAARSLQKVREQAARRASQGRRERGSQAKILLD
                     FKAEQAGKSLGRVLDRHERGHSAQVEARTEAAAALAAVQPLIMQWPEEIERKGAAQPLV
                     ITAASHVRWHGQRVDLVLQRGERCLLGGRNGCGKSSLLQMLRGVLEPETGSFRINGKMA
                     CLDQGLSLIARDLSPLANLLTVAPTLAESDARTRLAGIALRGDRALTPCHGLSGGERLK
                     LGLLMVLAQLPDLLLLDEPDNHLDHPSRQLLTQVLADYPGTLLLVSHDPDFVAGVGVGK
                     ELNLSE"
                     /name="ATP-binding cassette domain-containing protein"
     gene            complement(4044..4328)
                     /locus_tag="PUP32_RS00035"
                     /old_locus_tag="EBKDGGOF_00005"
     CDS             complement(4044..4328)
                     /locus_tag="PUP32_RS00035"
                     /old_locus_tag="EBKDGGOF_00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_273497494.1"
                     /translation="MGALAFYSYKKPGDRMNSDQSYWLVGASWDGVDHQDKKFVDNKIW
                     VLGYKEKDDESQYLKAKKIKKGDRIAIKGDGRENGQNRTLSMRPVRLRP"
                     /name="hypothetical protein"
     gene            complement(4887..5273)
                     /locus_tag="PUP32_RS00040"
                     /old_locus_tag="EBKDGGOF_00006"
     CDS             complement(4887..5273)
                     /locus_tag="PUP32_RS00040"
                     /old_locus_tag="EBKDGGOF_00006"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_240834510.1"
                     /translation="MRVSNVEWLKKRIAFIRKLGEKTARQRQIIDLLDDEASLSETERR
                     LLHVLATAEKNALQERDSAQKKAVQARISGQKNRRERNHRLFLAAGLMIDAGLVDTKTG
                     ELNFDHEKLLTALKRVRYDLETSA"
                     /name="hypothetical protein"
     Typing          3..349
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="4.84e-68"
     Typing          5442..6022
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.454"
                     /coverage="99.0"
                     /evalue="7.11e-127"
ORIGIN
        1 ttttattgat agccggtcac tgaaagcaca gggtatcgac cggaaaccgg aacagcatct
       61 cggttttgaa gcagccggac gactggacga cagccagagg caggatatta aagacagccg
      121 gacgttttca gtcgtggatg tgcctgaaca ggcgaagcat atcgaccaca gcgaccgtag
      181 ctggatgaat gccagcggta tcgaggtaaa ccggaaggaa ctggtcagat ctctgacgca
      241 tgatatctcg cagcagtacg ggcgggagat ggctgcacgg gtcacaaaac agggaaatgt
      301 tgaaatagag cgcgtgaaat ccctgtcacg cggattttcc ataagcaggt aactatggcg
      361 agcaccgaag aaaaacttct cacactcctg ctgtcggcgg agcatctgaa agaggccgca
      421 aaagagcagc agaaagccgt ggcggattcg ctggcggtgg caagggaaac cctgaaaggc
      481 taccgggaaa tatcagacag tgtggcacag caggtacggg atgaagtccg gcaggaagtg
      541 cagaaaatgg atgttgcggg gctgattggc gagcgtatca gcgctgattt tcagcgtctg
      601 gaaaaatccg tcgggaacat gaaaatcaat gcggacgcgc tggacaagga aatttccatt
      661 atccgcagta acatcatcgc ggagtacaac acgctgcggg gttacagctg gaagtggttt
      721 gctggggttg cggttgcctt tgtcgtactg atatttgcca caagctggtt tgtgaaaacg
      781 gaactacagg acaattacaa cgcgtctgct gccgtctatc agaaagtggc agaccttgaa
      841 aagctgctga caacggaaca gaaaccggaa aagaaaaaga actgacgcgc tgcgcttgtc
      901 caacgcctca cgttcgcaca ataaattgcg cacccgttca ccgttttcag ccgggtttgg
      961 ggcgtctttt gagacttccg gcctgaattc aggccggaag ttctcgccag tcagtcaggt
     1021 gcatggttat gcatggggct gaaaatcacg gtatgcgatt ctgagcggtt ttcggtgaga
     1081 agacgggggt ttttatggct caggacgtaa aaccgccctg agcgcgtttc agcggtgcgc
     1141 gtaatgaggt gttatggtaa atcatttact atcttttttt tcttcagatt tcccaaaaat
     1201 cagtcctaga gtcccaccaa tagctgcgcc aattccggtt ccaataacag ggacaatgct
     1261 ccctaatgct gctccagctg ctgctccttt gagaatctct tttttcgaac caggctccca
     1321 ttccaactcg gttgacgatg aaccgttgct attctctttt tttgtgaccc ttacaagacg
     1381 atctattttc atcatctgtt ctcccttttg ttttcgattg tcggctaatt acagtgagaa
     1441 tactttgttc agccatgcat cggtatcctt gtccttccct tgcttgtatg gaccgtgaat
     1501 gctggcaaag gcacctttac tgtcaacttc aatgttcttt tctagtacta gccaattgac
     1561 tacgcaaatg accaatgagc catagtcgac tactccttcc actattcctg tgtggagtat
     1621 ttttatgttg gctgagcctt ttcccatcat cctttttagg ggtcggctca gaaaacggaa
     1681 aaaatcgtac gctaagccga agttcagcac tcacgcattc tgtcgagcac gtaccagttc
     1741 cgtgaaactg acaacatcca tcaaccatgc ctgctgggct ggcgtatagg tctggtgcag
     1801 actgcgcggc agaacgagtt gcagccgctt tgctgccatt tcgtcggtct gatgcgtgga
     1861 gattgctgtt tccagcgtca gcagatgctt gtcatcaatg cggtctgctt cggcgagaac
     1921 ctgccgccag cggtccttgc aggtcgattt cacaccaagc atggtcagct tcagcgagtc
     1981 gaacgccggg ttatggtatt cggggttgta agccggaacc gccgaaattt ccgtcaactc
     2041 ctctaacccc ctgccacaag ggcatctagg tcaatcatct tgcggtcact ctgaaagatt
     2101 taattcttta ccaaccccaa cccctgcaac gaagtcggga tcgtgagaaa ccaatagcag
     2161 agtgcctggg tagtccgcca ggacctgagt gagcaactga cgcgaggggt gatccagatg
     2221 gttgtccggt tcatcgagca gcagcaggtc gggcagttgc gccagtacca tcagcagacc
     2281 cagttttaac ctctcccccc cactcagacc atgacaaggc gtcagagcac gatcgccgcg
     2341 cagggctatg cccgccagcc gtgttctcgc atcactctcg gccagtgtcg gagccactgt
     2401 caacaggttg gcaagcgggc tgagatcccg cgcaatcagg ctgagcccct gatcgaggca
     2461 agccatcttg ccattgatgc gaaagctgcc cgtctcgggc tccagcaccc cacgcaacat
     2521 ctgcagcaga ctcgatttgc cgcagccatt gcgaccgccg agcaaacagc gctcaccccg
     2581 ttgcagcacc agatcaaccc gctggccatg ccagcgcacg tgagacgcag ccgtaatcac
     2641 cagcggctgg gcagccccct tgcgctcgat ctcctccggc cactgcatga tgagcggctg
     2701 caccgccgcc agcgcggctg ctgcctcggt gcgggcctcc acttgcgcac tgtggccccg
     2761 ctcgtggcgg tccagcactc tgccaaggga tttgcctgcc tgttccgcct tgaaatcaag
     2821 caggattttg gcttgggagc cgcgctcacg ccgcccctga ctggctcgtc tggccgcctg
     2881 ttcacgcact ttttgcaagc tgcgcgccgc cgccttttcg cttcgcaccg cttgcgccaa
     2941 actggtgctg cgggcagcct gtacctcggc ctgctgtgcc agaaactcgt caaacgagag
     3001 gcgggcactc tgcaacccct gctcgctcag ctccagaata tgactggttt gattgagcag
     3061 gcgccggtcg tggctcacca gcaggtaacc tccgcgatgt ctggccagtt gctggcttag
     3121 ccagtcgatg ccagaggcat cgaggtggtt gtctggctca tcaagcaaca gaaagtgagc
     3181 aggcctcgca aacagcgcca gcaggcgcaa acgggtgagc tggccaccgc tcagggtgct
     3241 gaccgggaca tggggatcga gtctgaggcc tgcttctgcc agcaggtcgg caagatcgct
     3301 gcgcaggttc cagcgatcag ccagcaactc gaaatcgtcc tgggcgcagc tcccctgctc
     3361 gatgcgctca agggcccgta gcggctcgcc ccagccgata aggtcggcca cactggctgc
     3421 gcagtcatga tcttgagccg gtgcaaactg ggacaaccag ctgatggggg tcaaccactc
     3481 gatcttgcca gaatccgcgc ggcgctggcc cgccagcagt tccagcagca ggctcttgcc
     3541 gatgccgtta cggccgatca gggtgcagcc cgcagcgggc aaagtgaagg taaggggcac
     3601 agtacaggcg acgaagggca gctgcaaagt gttgatatga catgcgatat gggtcatctg
     3661 aactcctgta ggccaatgcg gaagccgcag gccaagagct aaaccaaatg acagggacac
     3721 gtgtccacta gtgggaaaac agcagaggaa tcagcatcaa cctgtcacgg tgagacgcac
     3781 ccgctctccc tggcgggaga agaatcaaaa gtggggtgcg gacaggttaa atgcgcatcg
     3841 gcgaaatact ctttactgat gaaagtaaaa tcatacttca gatagtaaaa aatgccaaat
     3901 gttccggtat cactcacttg catgttgtat caataagtca agtgtcagtc tgggctatac
     3961 ctcaactggg cgtcagtcgg ctcactggca tcgcgtcaga ctagggccgc tttctgcatt
     4021 atctgacgcc taaacgtcaa actctatggc ctcaacctga caggccgcat gcttagcgta
     4081 cgattttgtc cgttttctct accgtcccct tttatagcga tacgatcgcc tttttttatt
     4141 ttttttgcct ttaaatactg actttcatca tctttttctt tgtagcccaa aacccagatt
     4201 ttgttatcta caaatttttt gtcttggtga tctacaccat cccagcttgc acccacaagc
     4261 cagtaacttt gatcgctatt cattctgtct cctggttttt tataagaata gaaagctaat
     4321 gcccccatgg tgggtatata aaaaccatgg ggagcggagc gaccttcgtc aagctctttt
     4381 cttgtgtgtc ttatccagca actgattcga tttgtgtggg gttagtgtca gacttctcac
     4441 aatcacactt tcccgcttac tgctgccttg ttcgctcatt ttatcaggtg cttgttatcg
     4501 tacagcgaga cggggtagag cctaactctt tcgcggttaa gttacgttcc caccaatttg
     4561 cctgtttatc ctgccggagt ccggggcgta gacctgagcg tctaaggtgc agatatgatt
     4621 ttaatgtcgt tgaatggtat taagctggta gtgacatggc tagccagccc tgctaagatt
     4681 tgattgtgac gtcttatcct ggcaggtatc tgcaagaatt agagtactca agccccgtga
     4741 ttctgtcacg gggcttttgt acatctggag caagaaaatt catgctccta tccgaaaatc
     4801 cctaccgctc gccgcagccg aacgaccgag cgacgcgagt cagtgagcga ggaagcggca
     4861 tttctccggc cttgcagaat gcacgttcac gccgatgttt ccagatcgta gcgtacccgt
     4921 ttcagcgcag tcagtaattt ttcgtggtca aaattcaact cacccgtttt tgtatcaacc
     4981 agtcccgcgt caatcatcag accagccgcc agaaacagtc tgtgattgcg ttccctgcgg
     5041 tttttctgcc ctgaaatccg cgcctgaacc gcctttttct gtgcggaatc ccgttcctgc
     5101 aacgcgtttt tttcagcggt tgccagaacg tggagcagtc gtctttcagt ctcactgagc
     5161 gaagcttcat catccagcag atcgataatc tgccgttgcc gtgccgtttt ttctcccagc
     5221 tttctgatga aggctatgcg ctttttaagc cattcgacgt tactgacccg catcgtttaa
     5281 cttccccatc ccgatatttc cgacggggaa agtatataac ttgcggtcag aagtcgcatg
     5341 acttagcttt actcataatt ctttaaatta gagtaaagcg cacttatatg tttctccctg
     5401 cgggcagaaa cacgtgcgta aaaccaaggg caaaaacatc atggcaacct atcatctcag
     5461 cgtgaaattt ggcggcaagg gtaaagccct tgctcatgcc aattacatca cgcgggaaga
     5521 taagttcagt cagcgaaaag atctcgaaca caccgagcac ggcaatatgc cggaatgggc
     5581 gaaagatgac cccgcccatt tctggcaggc tgcggatgcg ttcgaacgtg ccaacggttc
     5641 aacgtaccgg gaaatcgaga tagcactacc ccgtgaactg aacgaagcgc agcggctggc
     5701 gcttgtcagg gattttgtga agcaggaaac gggcggtcaa catacgtatt cgtttgcgat
     5761 tcacaattcg caagccagca ttgacggcgg cgaacagccg cacgcccata tcatgatgag
     5821 ccagcgtgtt gatgacggta ttaagcgcag cccagagcag tatttcaggc gctataacgc
     5881 caaatttccg gcgcgtggcg gcgcgcgcaa ggactcaggg cataatttaa cccctacaga
     5941 acaaaaacag gcactgaaag ccctgcggga gcgctgggag cgtacgcaca acgcgcatat
     6001 gaaacgatat ggctttgagc aggg
//
