LOCUS       NZ_OP242308             3285 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA189 plasmid pLAO57, complete sequence.
ACCESSION   NZ_OP242308
VERSION     NZ_OP242308.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363359
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600925.1-RS_2024_03_23
            Annotation Date              :: 03/23/2024 03:28:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 4
            CDSs (total)                 :: 4
            Genes (coding)               :: 4
            CDSs (with protein)          :: 4
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242308.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA189"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO57"
     gene            complement(95..532)
                     /locus_tag="PUP04_RS00005"
                     /old_locus_tag="NJKOJCND_00001"
     CDS             complement(95..532)
                     /locus_tag="PUP04_RS00005"
                     /old_locus_tag="NJKOJCND_00001"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_046597188.1"
                     /translation="MKLKGFWLSAIVIAACNGMGFLFLYLSKTYFFPMVTFKDNLGNLG
                     SSLVTYSTAMMALLIAMVVIIFGLDGPRLHNFKKSGYLHVTYFIYMITFFELGLTMALS
                     LLCLSNISSIKVASYSLTFAFITFLLIGCLGIQLLNIKKNL"
                     /name="hypothetical protein"
     gene            complement(543..1376)
                     /locus_tag="PUP04_RS00010"
                     /old_locus_tag="NJKOJCND_00002"
     CDS             complement(543..1376)
                     /locus_tag="PUP04_RS00010"
                     /old_locus_tag="NJKOJCND_00002"
                     /inference="COORDINATES: protein motif:HMM:NF027299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072775497.1"
                     /translation="MILLAGKKMKTNYFTHRLFPSDGAVICEPLKTSFDNMIATNRNTA
                     TLANEYHVIQHVSGDVYTFIKTNSRDVFRKLDTSSNICVDLSNILNQHEKIAFASFFIL
                     KGDVIGFSNTLYSPRVGKLAEIYDAAMYSRNGNHNINFEPITNIVTEQDVMNYAHVGKI
                     TMKIEKSQGIIGGLGTLFSGNVKYDDVDSFEIKIIPKRAKDIKDTFAGLMQARPQEVSS
                     VAVSAKEHIGDVATDLNVIMSNTVYDFVNPNDTTTIEVQMEKNYNNNTTLRSLGY"
                     /name="hypothetical protein"
     gene            complement(1546..2004)
                     /locus_tag="PUP04_RS00015"
                     /old_locus_tag="NJKOJCND_00003"
     CDS             complement(1546..2004)
                     /locus_tag="PUP04_RS00015"
                     /old_locus_tag="NJKOJCND_00003"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272954263.1"
                     /translation="MTKKRSSLYRSCLHERVILPDYRITSKVHQNYITFTSLLHHLYIT
                     FTSFLQLIRHFHPLCHLYITPFLNVLTAGFPAAPATLHGHAINCVPVHRFRVRRTSETG
                     LTPAEWCEILRYALARILMHAVKTEPARFWRVFGWFVACFTGFPSETR"
                     /name="hypothetical protein"
     gene            complement(2244..2393)
                     /locus_tag="PUP04_RS00020"
                     /old_locus_tag="NJKOJCND_00004"
     CDS             complement(2244..2393)
                     /locus_tag="PUP04_RS00020"
                     /old_locus_tag="NJKOJCND_00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     Typing          complement(2387..2601)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.908"
                     /coverage="100.0"
                     /evalue="7.28e-73"
     Typing          2577..3063
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.081"
                     /coverage="93.0"
                     /evalue="5.84e-177"
ORIGIN
        1 tctcaagacg atccttacac attctgcatt ttatcaactc accaaaaatg gtcacaaacc
       61 gtttcacctg tgatgaacat cacagtttgg ttgattaaag attctttttt atatttaaga
      121 gttgaatacc taaacatcct atcaacaaaa aagtaataaa tgcaaaagtc aatgaataag
      181 aagccacctt aatacttgag atattggaca aacaaagtaa cgaaagagcc atggttaagc
      241 cgagctcgaa aaacgttatc atataaataa aatatgtaac atggagatag ccgctttttt
      301 taaagttatg caagcgaggt ccatccaagc caaagattat aacaaccata gcaataagca
      361 acgccatcat cgctgtcgaa taagtaacca aggaagaacc aaggttacct aaattatcct
      421 taaacgttac catcgggaag aaataggttt ttgaaaggta tagaaataaa aagcccatgc
      481 cattacatgc agctataact atagcgctca accaaaaacc cttaagcttc acaatgcaat
      541 cctcagtaac ctaaacttct taatgttgta ttgttattat aatttttttc catctgaacc
      601 tcgatagtag tagtgtcatt tggatttaca aaatcatata cggtattaga cattataaca
      661 ttcaaatctg ttgcaacgtc accaatgtgt tctttagctg acacagcgac cgagctaacc
      721 tcttgaggcc tagcctgcat caaacctgca aaggtatcct ttatgtcttt agcccgttta
      781 gggattatct ttatctcaaa cgaatccaca tcgtcatact ttacgttccc agagaaaagc
      841 gtaccaagcc caccaataat gccttgagac ttctctattt tcatcgttat tttaccaaca
      901 tgggcataat tcataacatc ttgctcggta actatgtttg ttatcggctc aaaatttatg
      961 ttgtggttcc catttcggga atacattgca gcatcatata tttctgccaa cttcccaaca
     1021 cgaggagagt acaacgtatt actaaaccct ataacatcac ccttcaatat gaaaaaagat
     1081 gcaaaggcta ttttttcatg ttgatttaag atgtttgaga gatctacaca tatattacta
     1141 ctcgtatcta gttttctaaa aacgtctctg ctatttgttt ttataaacgt atatacatcg
     1201 ccagacacat gctgaattac atgatactca ttggctagcg ttgcagtgtt tctgttagta
     1261 gcgatcatat tatcaaaact agtttttaat ggctcacaaa taacagcgcc atctgaagga
     1321 aacaatctgt gtgtgaagta atttgttttc atttttttcc cagctagtaa aatcatagaa
     1381 acgcattata ttttcttctt tttatattct atttcttatg gaagaacaat gcttctttaa
     1441 aattccaagt tttgcatatt aaatgatctg tcaccgaagg tcgtaaagct aggccaccaa
     1501 ctctatttac cataacatcc gttgcgtgca ccgccgcaga ggccctcagc gcgtttctga
     1561 cgggaaaccg gtaaaacagg caacaaacca cccgaaaacc cgccagaaac gcgcaggctc
     1621 tgtttttacg gcatgcatga gtatgcgcgc aagtgcatag cgcagaattt cacaccattc
     1681 agccggggta agtcctgttt ccgatgttct tcttacccga aaacggtgaa cggggacgca
     1741 atttattgcg tggccgtgaa gcgttgccgg cgcagccgga aaacccgctg tcagcacgtt
     1801 caggaagggg gtgatgtaca aatggcacag aggatgaaag tgacgtatga gctgaaggaa
     1861 tgatgtaaaa gtgatgtata agtgatgtaa aagtgatgta aaagtgatgt aattttgatg
     1921 tacttttgat gtaattctgt agtcaggaag aataacccgt tcatgcaggc atgaacgata
     1981 cagtgaggaa cgcttttttg tcacgatctc cccttaacct gcgtaaatca cccgccatgg
     2041 ttgattattt ttcaacctca aaatggtcat acacctaacg cagccgcgat tttcatcgca
     2101 actgcatgaa ttaaaagaaa aaaaaggaat atttcgtttt cacgagtaat acccgggtat
     2161 taaaaatcca ctgtaattac atgaaaataa agaaataaaa ggtaataccg gagtattacc
     2221 acgtacgcgg gcaagcgaaa aagctataac gcttcccggc cgacaggcga cccaaaatcc
     2281 acaggggcat gcaggttctc ttcagtaacc tgcgccatta aatcaaaaag gtcatattcg
     2341 ccgggaagct gcgtatcagt cgaatccagt tgttcatcat attcagatgg catggggtga
     2401 tatccagtag ctgagaggtt gttagcgggt ttcggggcgc agccctgaac cagtcacgta
     2461 gcgctagcgg agtgtataca ggctgaatat gttaggtcgg agtggtaatt cagggaagtt
     2521 attcatgttg caagggaaaa tgcggctata ggacgtaagt gcaaaatgca ggtaacggtg
     2581 acatgacgcc tcctcgctca ctcggtcgct acgctcctgc cgtgagactg cggcgggcgt
     2641 tacctgctca cgaatgacaa gataaaaaga tggctctcaa gtcaggaaag gccggaaaaa
     2701 ggatacggcg ctgccgtttt tccataggct ccgcccccct gacaagcatc acaaaatctg
     2761 acgctcaaat cagtggcggc gaaacccgac aggactataa agatcccagg cgtttccccc
     2821 tggtagctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct
     2881 gttatggccg cgtttatctc attccacgcc tgacactcag ttccgggtag gcagttcgct
     2941 ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ctactgcgcc tttccggtaa
     3001 ctgtcttctt gagtccaacc cggaaagaca cgacaacgcg ccattggcag cagccattgg
     3061 taagaggatt cacggagcag agtagtagag tatttctatg gagttcttga agtggtggcc
     3121 tgactacggc tacactaaaa ggacagtttt ggtatctgcg ctccgtgaaa gccagttacc
     3181 ttcgggagaa agagttggta gctctcactt ccggcaaaca aaccaccgtt ggtagcggtg
     3241 gtttttttgt ttacagagca agagactacg ctgcactctc aaaaa
//
