LOCUS       NZ_OP242304             1917 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA243 plasmid pLAO99, complete sequence.
ACCESSION   NZ_OP242304
VERSION     NZ_OP242304.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363371
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600885.1-RS_2024_03_23
            Annotation Date              :: 03/23/2024 03:25:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 2
            CDSs (total)                 :: 2
            Genes (coding)               :: 2
            CDSs (with protein)          :: 2
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242304.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA243"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO99"
     gene            join(1808..1917,1..193)
                     /locus_tag="PUP17_RS00005"
     CDS             join(1808..1917,1..193)
                     /locus_tag="PUP17_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="WP_031942343.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIAAALLA
                     FFLGGFGAHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     gene            complement(453..689)
                     /locus_tag="PUP17_RS00010"
                     /old_locus_tag="NLCIELOF_00001"
     CDS             complement(453..689)
                     /locus_tag="PUP17_RS00010"
                     /old_locus_tag="NLCIELOF_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          complement(855..1028)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          1007..1498
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 tagaatcgca gccgcgctat tagcattctt ccttggtgga tttggcgcac ataaatttta
       61 cttaggaaaa ataggacagg ggttcctgta tctaattttt tgctggacat tcatacccgc
      121 gatcatcgcg tttattgagt ttattattta tctatgcgat tccgatgaaa aattcgcacg
      181 aaaatatggc taactcatag caaaaaaaag cccttagggg gctttttttt gtacctgacg
      241 gtcagaccga ttaaacataa catcccttgc ccgcaccacc gcaggcgccc tcagcgcaat
      301 cctgccgcat cagggccgtt tccggcacct ttcctcacca gtatcccgga aaaacgcccc
      361 tgatgatttc tgcgcgactg catggctatg cacgcaggtg aatggcaggt gatttcgcag
      421 cgtcagcacc actaaaccgc caaaagccaa aatcagcggc gcggcgcgcg cggacgtgac
      481 caggcaaaat cggactgctg ccgctcatcc tcacgtaatg cacgctggcg gtcggcgtaa
      541 cgctgcaact caatttgcgc acgcgctctg ttcaggacga tgtgccggtt aggctgattt
      601 agctttttac ctgacgctct ttcagctgcg gcaacagcat catcaagaaa ttttttctct
      661 gcaggcgtca agtttgtcag tctggccata acgttctcca gtcagaataa atcagcacag
      721 tctgccagac acagtagaaa cgatgaagcg ccagcgacgg tgaaaggcag tcagaaacgg
      781 tgaacgggag cggattttaa tccggtgccg tgaggcgttg gacaagccgc aggcgcgtca
      841 ttagctttta gcgggtttcg gggcgcagcc ctgaaccagt cacgtagcgc tagcggagtg
      901 tatactggct gactatgttg gcgcggatgt ggaaatcagt gaagtgcttc atgtagcagg
      961 agaaaaaagg cggcaccagc gcgtcagcag gatatgtagt acaggatata ttccgcttcc
     1021 tcgctcactg actcgctgcg ctcggtcgtt cgactgcggc gagcggtgcc ggcttacagg
     1081 cggggcggaa atttcctggg agataccagg aaggagctta acagggaaga gagaaggccg
     1141 cggcgaagcc gtttttccat aggctccgcc cccctgacaa acatcatgaa atctgacgct
     1201 caaatcagtg gtggcgaaac ccgatgcggc ttaaagatac caggcgtttc cccctggtgg
     1261 ctccctcgtg ctcccccgca ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
     1321 gccgcgttta tctcattcca cgcctgacac tcagttccgc gaaggcagtt cgctcctgga
     1381 tggactgtat gcacgaaccc cccgttcagt ccgactgctg cgccttatcc ggtaactatc
     1441 atcttgagtc caacccggaa agacacgact tatcgccact ggcagcagcc actggtaact
     1501 gatttagagg agttagtctt gaagttatgc gcctgttaag gctaaactga aagaacagat
     1561 ttggttactg cgctcctcgt aagccagtta ccacggttca aagagttgct gctctgagaa
     1621 ccttcgaaaa accgccttgc agggcggttt tttcgtttac agagcaagag attacgacgc
     1681 cacagaaagg atctcaagaa gatccttcac cgtaatggaa atctatccaa tccgagcaca
     1741 taggatacac atcgttataa aacatcggat attatgatcc caattaaaat aagctcgagg
     1801 ataaaaaatg agttcaatgg tttactgccg aggatgtggc aaggaaatac acgaaacagc
     1861 aaagtcatgc ccgcactgcg gagccacaaa cgcctcttca ggttcaggtg aaaaaag
//
