LOCUS       NZ_OP242302            10248 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA243 plasmid pLAO97, complete sequence.
ACCESSION   NZ_OP242302
VERSION     NZ_OP242302.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363371
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10248)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10248)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600865.1-RS_2024_03_23
            Annotation Date              :: 03/23/2024 03:27:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 13
            CDSs (total)                 :: 13
            Genes (coding)               :: 13
            CDSs (with protein)          :: 13
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242302.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA243"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO97"
     gene            join(9275..10248,1..196)
                     /locus_tag="PUP17_RS00005"
     CDS             join(9275..10248,1..196)
                     /locus_tag="PUP17_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272955216.1"
                     /translation="MAFDNSHLEDAGLFWRAVSSAPAPVDRDEWLKMARGIACFASLCG
                     EDGLAAIEAWSTRHNRGEAKHRSQYRHFLKLKGITNPEAMAQVFLQFSGVEWRTGRASV
                     QSDVDRAERLRLSLEATAKAKAERDKKGDAAARAAVKLLAKGGDPASTPYWRKKTALAA
                     AGGVQLPALSTDLGVGGMFRSFNPRAYWSDCLLIPVYKFDAARQLYVDGLEAICGRCSA
                     EGSRVMGTKFTPAGTRKAGGFYPMPGFMKVLDTSPHAPYSPLFVCEGFATAVALHHLSR
                     GKVAVCAAFSIAGFGSCVASLRRFWPDVDLMLVPDHGEAYRLAHKAAYGDEREPVPTIP
                     GAAVVSFDGLDRFTPRDNLDWFDVLVEEGEDRARELLRYAVRHARLERG"
                     /name="hypothetical protein"
     gene            453..914
                     /locus_tag="PUP17_RS00010"
                     /old_locus_tag="DOPNJCKG_00002"
     CDS             453..914
                     /locus_tag="PUP17_RS00010"
                     /old_locus_tag="DOPNJCKG_00002"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272955217.1"
                     /translation="MKSKTELRAAATARSLAVIATEMSEWSLDGFSHLKLPNFSPLPRQ
                     QTQEGSMVLERPPFDYTWAGTAAFTDRANRALQVKLPASRERNYIWLCGVERETLATAL
                     LVELFSVTDCVEFAGLGKVADLAFLTLDEADAGQIRAAMVGWLDDPAAA"
                     /name="hypothetical protein"
     gene            1266..2648
                     /locus_tag="PUP17_RS00015"
                     /old_locus_tag="DOPNJCKG_00003"
     CDS             1266..2648
                     /locus_tag="PUP17_RS00015"
                     /old_locus_tag="DOPNJCKG_00003"
                     /inference="COORDINATES: protein motif:HMM:NF017116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="WP_272955218.1"
                     /translation="MTRAKGVFPVIPRRCKAPRDWLFNFEDVKVSNHEENSGTAADNVT
                     PIAAAKVGRPSLQKKYPTTAFPVELEINYRKGRYSSSLENVVAAMRCHSVTGIQLAFDE
                     FFAEEGAFVNGQWYPLSDNLVTELRLRLAAADFEKVTKDNARDAMFYVCDENKFDSAQR
                     WLTAQEWDGKPRVSYFFERCFKCQGDPEYLAAVSLYTWTALAGRVMNPGIKADMVPLLY
                     GRQGARKSTGVRLMSPFPDSYSSFNLNDKDDSLVRRMRGCLIGELEELRGLHGRDSESV
                     KAFFSAAHDEWVPKFKEFKVRNPRRVVFIGTTNNKQILNDSTGNRRYLPMNSGDVDCAA
                     IEKNRSQLWAEALHLFNENGVMWEAAETLARKVHDEFRVEDPWEDEIAELLKIGVDDCE
                     PTSFVPTKALFSQVGLMIGHTSNKQHQRMAAIMSRLGYEVGRKRINGALVRGYFRRTQA
                     VV"
                     /name="VapE domain-containing protein"
     gene            complement(3372..3839)
                     /locus_tag="PUP17_RS00020"
                     /old_locus_tag="DOPNJCKG_00004"
     CDS             complement(3372..3839)
                     /locus_tag="PUP17_RS00020"
                     /old_locus_tag="DOPNJCKG_00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_268557221.1"
                     /translation="MKKYTTEQKAQALRLLEQDGATSATVARTMGIPPRTVRRWASEKA
                     AAPSNVLSIEEMRKRAAAAVEATPQAAIRRLKNHFVQRQYELLQRHASDLQALRSASLQ
                     AMLEKDATMVKAISGLMTSLLKAQERERLIYEIKPGTEADIMREGMNRKQQ"
                     /name="transposase"
     gene            complement(4085..4276)
                     /locus_tag="PUP17_RS00025"
                     /old_locus_tag="DOPNJCKG_00005"
     CDS             complement(4085..4276)
                     /locus_tag="PUP17_RS00025"
                     /old_locus_tag="DOPNJCKG_00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060634173.1"
                     /translation="MTPGRTQILKATADIAKALGTDTADVERHFLQHAVVLARLRQEGK
                     RPRVKAWMAENIGTPSAM"
                     /name="hypothetical protein"
     gene            complement(4279..4470)
                     /locus_tag="PUP17_RS00030"
                     /old_locus_tag="DOPNJCKG_00006"
     CDS             complement(4279..4470)
                     /locus_tag="PUP17_RS00030"
                     /old_locus_tag="DOPNJCKG_00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065945242.1"
                     /translation="MQSIQQTNPARYPGLSIEHGTITQHGRARAVIMNRRGIHQVSLRT
                     AEGRTVTLPVCRLFQWEA"
                     /name="hypothetical protein"
     gene            complement(4575..5150)
                     /locus_tag="PUP17_RS00035"
                     /old_locus_tag="DOPNJCKG_00007"
     CDS             complement(4575..5150)
                     /locus_tag="PUP17_RS00035"
                     /old_locus_tag="DOPNJCKG_00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151785.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_101998997.1"
                     /translation="MSRVFGYGRVSTGEQTVAGQKKAILDRYPQATFHADEGISGTVPA
                     TQRDKFKAILEFAAPGDSIVVVAIDRIGRNARSVMEVCEELEKRQVSLISLREGFDFST
                     PTGKMMLTMVAAFAEMELATLKERTKAGMAAAAAAGRHAGRPTKQTPENVGAVIMKHDA
                     GVSAARIARDLGISRGTVNRILQKHAKA"
                     /name="recombinase family protein"
     gene            5401..6060
                     /locus_tag="PUP17_RS00040"
                     /old_locus_tag="DOPNJCKG_00008"
     CDS             5401..6060
                     /locus_tag="PUP17_RS00040"
                     /old_locus_tag="DOPNJCKG_00008"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272955211.1"
                     /translation="MTEIKKESADGAWLRSTLAELAGDAFPVYDLPCLSVDPNSSAQLA
                     ALDDRRVAREMRQAAADVETRRIDAARVVEGLKTEAERLRGLIADGKAALRAGETVSPD
                     AGVASFLLPDIEAELVVAEAAEADVARERDGLLQDADRRDAAAALTLFNWAHSVRVQRI
                     ELLLKLAMDEATLLAESDAGRSLYRTVINPDRRLNQLFSTQGAIEILRRNRGMEGV"
                     /name="hypothetical protein"
     gene            6060..6443
                     /locus_tag="PUP17_RS00045"
                     /old_locus_tag="DOPNJCKG_00009"
     CDS             6060..6443
                     /locus_tag="PUP17_RS00045"
                     /old_locus_tag="DOPNJCKG_00009"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272955212.1"
                     /translation="MSAFKLLCDRLRHEATLAKSTDAGDALAQVLETGGAVQTFAPIGA
                     PVASPTEAVHRARAQSIQDAAFLNEMKAAGVAPADLVMLDGVLTSSRGMPDAARAPAFA
                     AVRAQVQQQASLSPAARGINWDE"
                     /name="hypothetical protein"
     gene            6449..6604
                     /locus_tag="PUP17_RS00050"
     CDS             6449..6604
                     /locus_tag="PUP17_RS00050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272955213.1"
                     /translation="MPRFVLHEPSLYSWGVFCRGSASDAGVKAAGDLLDQHAFALAIST
                     TIGGGH"
                     /name="hypothetical protein"
     gene            complement(6806..7456)
                     /locus_tag="PUP17_RS00055"
                     /old_locus_tag="DOPNJCKG_00010"
     CDS             complement(6806..7456)
                     /locus_tag="PUP17_RS00055"
                     /old_locus_tag="DOPNJCKG_00010"
                     /inference="COORDINATES: protein motif:HMM:NF016255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_272955214.1"
                     /translation="MTEPTRPILTIKRRNRTVYVPPEKAAKSEPQPAERTTSKAKVQPK
                     AKAPQKPAQKPTAAKPKKTPEQVAEGIRRQEENDRRQQDIAIANKSKKALANRVTKAFL
                     KEHWPDLLTRGVDRRPMAKGIIEQILNRGEELGMPPETDRNAVRRILRIITGTSAYQQS
                     LIDQCMRYDLDGNPTEPATPEEAENAKARLTEIKAFRRKARQERQARDKKKGA"
                     /name="ProQ/FINO family protein"
     gene            complement(7453..8736)
                     /locus_tag="PUP17_RS00060"
                     /old_locus_tag="DOPNJCKG_00011"
     CDS             complement(7453..8736)
                     /locus_tag="PUP17_RS00060"
                     /old_locus_tag="DOPNJCKG_00011"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272955215.1"
                     /translation="MNDDNDTNESTQPNPAALERVGATEKFPEQLPGVLFVTCPGGCSL
                     RVEACRQVYQRSHEDKSIDNPHLECRRCPIGFALWSGIEQDNEAPVTTLPETRNLCPRC
                     SRTTNRLIAPNKPGSSLCVSCWNREREASTGVNARGRVNRTPPFVSPFLIGIGDGWQVW
                     YGIHQGEVLIRAMRANQGARFHDRRPGPVAATPGGFTYLCPEHGKPLRWDTDEHQGVVS
                     FACPSCTPTASALSVAPVMQPVTIQTAQDAAYQYRNATLPDNAVWTASVCARCRTAPLI
                     IGKAPVKGKPRRTITVNCPACGDSATDRPGGRTWTDEYIAVPEFAQNETRSLAKIPVVL
                     PQKERAQPLKRPGTGRSPGRPRKRIPLTLERVPESRFNRLTLSVLAAVGRWDYINDIIR
                     RIDRDRGDAATLIALADHIQTNRPERDL"
                     /name="hypothetical protein"
     gene            9079..9288
                     /locus_tag="PUP17_RS00065"
     CDS             9079..9288
                     /locus_tag="PUP17_RS00065"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064186834.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYWL
                     AGNPEIKRFVADQRRPGRAADGVR"
                     /name="hypothetical protein"
ORIGIN
        1 taaagcggcc tatggcgatg agcgagagcc ggtcccgaca attccaggcg cggcggttgt
       61 gtcgttcgac ggcctcgatc gcttcacgcc ccgggataac ctcgactggt tcgacgtgct
      121 ggttgaagag ggcgaggatc gggcacgtga gctgctccgg tatgcggttc gtcatgcccg
      181 gcttgagcgg ggttgatggc tgggcttttt tgcgtcggtt tccccgggag gccgctgacc
      241 gtaaagggtc gttttctggc tcgtcttttt tgcagtcgct tttgggttgg tgagacaacc
      301 tttaagacgc acatccgacc gcttccggtt tcctccgttt tagacgccgc tgcggtcgtg
      361 acgctatcct gcagctggtt tggtttccag cagcacactc aagcccggca ccgttttggt
      421 gtcgggcttt ttttcgcccg taaggagggc acatgaaatc aaagactgaa ttacgcgctg
      481 ccgcgacggc tcgttcgctg gcggtgatcg ccactgaaat gtcggagtgg tcgctcgacg
      541 ggttctccca cctcaagctg ccgaacttct ccccgctgcc gcgccagcag acgcaggagg
      601 gctctatggt gctcgaacgc ccgccgttcg actacacctg ggcgggtacc gctgcgttta
      661 ccgatcgtgc aaaccgtgcg ctgcaggtga agctaccggc gtcgcgtgag cgtaactaca
      721 tctggctgtg cggggtggag cgggagacgc tggcgacggc gctcctggta gagctgttct
      781 ccgtcaccga ctgcgtggag ttcgccgggc tgggtaaggt tgccgatctg gcgttcctga
      841 cgctggacga ggccgatgcg gggcagatcc gggcggcgat ggttggctgg ctggatgacc
      901 cggcggcggc gtaggccgtc gcttctctgc gtgcaattgt cgtgcaggaa ggggtgccgc
      961 ccgggcccgt ggataacttc tgtggataag tattttgctg atggctggcc cgtggataag
     1021 tctcgcgatg gcgcgtggct gtaggatcag gcgcggatcg cgtttctcgg tgccggatcg
     1081 ttgtgtactg gcgcaggggg ggcaggtaac ggtatgatgg cgcggctgaa aaagaaaaac
     1141 ccgccttggg gagacgggtt ttcttcaaag ctgttttgag tttggagcct tcacggcggg
     1201 caccgtggaa aacacataat aactcatgtt gggtgtgaaa ccagcatgaa ttatacgcga
     1261 tcataatgac gcgtgcaaag ggtgttttcc ccgttatccc ccgccgttgt aaggctcctc
     1321 gagattggct gttcaatttt gaggatgtaa aagtgtcaaa tcatgaagaa aattccggca
     1381 ctgctgccga caacgtaacg ccgatcgccg ctgccaaggt gggacgcccg tctctacaaa
     1441 aaaaataccc gactaccgcg tttcccgtgg agctggagat caactaccgg aaggggcgct
     1501 actcgtcctc tcttgagaac gttgtcgccg ctatgcgctg ccattccgtc accggcatcc
     1561 agctcgcttt cgatgagttc tttgcggagg agggggcgtt cgtgaacggg caatggtacc
     1621 cgctgtccga caacctagtt accgagctgc gtctgcgcct cgctgctgcc gactttgaga
     1681 aggtgaccaa ggacaatgcc cgcgacgcca tgttctacgt ctgcgatgag aacaaattcg
     1741 acagcgccca gcgttggctg acggctcagg aatgggacgg caaacctcgc gtcagttact
     1801 tcttcgagcg gtgcttcaaa tgccaagggg atcccgagta cctggcggcg gtcagcctct
     1861 acacctggac ggcgcttgcc gggcgcgtaa tgaatcccgg aatcaaggcc gacatggttc
     1921 cgctgctgta cgggcgacag ggtgcccgca aatcgacggg cgttcgcctt atgtcgccat
     1981 tccctgactc ctactccagc ttcaacctga acgacaagga cgactcccta gtgcgccgta
     2041 tgcgtgggtg cctgattggc gagctggagg agctgcgcgg cctccacggg cgcgactccg
     2101 aatcggtgaa agcgttcttc tccgctgccc acgacgagtg ggtgccgaaa tttaaagagt
     2161 tcaaggtgcg aaacccgcgc cgcgtggtgt tcatcggtac cacgaacaac aagcagatcc
     2221 tcaatgactc gacggggaac cgccgctatt tgcccatgaa ttccggggac gtggactgcg
     2281 cggccatcga gaaaaaccgc tcgcagctgt gggctgaggc gctccacctg ttcaatgaga
     2341 atggggtgat gtgggaggcc gctgaaacgc tggcgcgtaa ggtgcatgac gagttccgcg
     2401 tcgaggatcc gtgggaagac gagatcgccg agctgctcaa gatcggggtc gacgactgcg
     2461 aaccgaccag cttcgtgccg actaaggcgt tgttctctca ggtagggctc atgattggcc
     2521 ataccagcaa caagcagcat cagcgcatgg cggctatcat gtcccgattg ggctatgagg
     2581 ttggcaggaa gcgcataaac ggtgcgttag ttcgtggcta cttccgtcga acgcaagctg
     2641 tcgtgtaagg gttacttttc cgtaagaggg gagctatatg ctcccctttt ttattgttcc
     2701 acccgggtgg atcacgggtg gatcatcacg ccgtttttgt accacctgtt ccacccgggt
     2761 gttccacccg ttccacctgt gttccgcccg tggatggaac agctaaggcc tagtgtttaa
     2821 aggctttcgt tgttgtgttc cacccgttcc accctatttt ctaattaaaa acaaaaaatg
     2881 aataaattgg gtaaataggg ggtaaatgct tgctaattga acaattagtt gtttttttta
     2941 ctatttatga atctatgccg gatcgggtgg aacgggtgga tcatcttgga attgacgcgc
     3001 cgcagctgca ccctgcggga tgcactttgc ttgtcttggt cgatcggtgg atgggttcgt
     3061 cgtgtcgggg tggtgggtgg tggtggttag ggggtggatc gggtggttgg ggtggcgctg
     3121 agggcacgag ctgtcgctcg cgctgggtgt tgcctgttgg tgttgcgtga ggccaatgcc
     3181 tcgcgccctc tgcggcttgt gtggaatgcg tagctgcagc tgcgcgtttg gttcgtccgg
     3241 gcatcaaaac ggggcttttc gcgcacgcga gcggtaacaa ttgcgtaacg tgcggatccg
     3301 cgtattccgg gggctcccgg gttttggcgg tcacaaaaac ggtcacatgg cggtcatttc
     3361 ggtcatggcg gtcattgttg tttgcggttc atgccctcgc gcatgatgtc ggcctcggtg
     3421 cctggtttga tctcgtagat gaggcgctcg cgttcctgcg ctttaagcag ggaggtcatg
     3481 aggccgctga tcgctttgac catggtggcg tccttctcca gcatggcttg cagggatgcg
     3541 ctgcgtaggg cttgcaggtc gctcgcgtgg cgctggagta gctcgtactg gcgctgcaca
     3601 aagtggtttt taaggcgtcg gatcgcggcc tgtggtgtgg cctcgacggc ggcggcggcg
     3661 cgtttccgca tctcctcgat gctgaggacg ttgctgggtg ctgcggcctt ctcgctcgcc
     3721 catcggcgaa cggtgcgggg tgggatcccc atggtgcgtg cgacggtggc actggttgcg
     3781 ccgtcctgct ccagtagcct gagcgcctgg gctttctgtt cggtggtgta tttcttcatg
     3841 gttcctgctc tggttggttt ctctcccgta ttgaaacgtc acgcgctgaa aatggcgggg
     3901 gtttttgggg ggtacacggc gtttgtgcgt gcgtggtcgt gcggtggcga gaaggactgc
     3961 ggcgatgatg gccttggttc tcatgctatg tcctgtgggg gcgctcctcg cgccccgggt
     4021 gtatggtttt cgttaatgcg taagtaaaac cgtatgctgt tttaccttaa cgtttaagtt
     4081 tttcttacat tgctgacggg gtgccgatgt tctctgccat ccacgctttg acgcgggggc
     4141 gcttgccctc ctgccggagc cgtgcgagga cgacggcgtg ctgcaggaag tggcgctcta
     4201 cgtcggcggt gtcggtaccg agtgctttgg ctatgtcggc ggtggctttc aggatctgcg
     4261 ttctgcctgg ggtcatgatc acgcctccca ctggaagagt cggcaaacgg gaagggtgac
     4321 ggtgcgcccc tctgccgttc tgaggctgac ctggtggatg cctcggcggt tcatgatgac
     4381 ggcgcgtgca cgtccgtgct gggtgatggt gccgtgctcg atggataggc ccgggtagcg
     4441 ggcagggttg gtctgttgta tgctctgcat gtcgtttacc tttgctggtt gatgatgccc
     4501 gggtcgctta cgagggcaag cccgggctgg ggttgcggcg gtgactttag cgagtaccac
     4561 cgccgcgctc tctcttacgc tttcgcgtgc ttctgtaaaa ttctgttcac cgttcccctg
     4621 ctgatgccta ggtcgcgtgc tatgcgtgcg gcgcttactc ctgcgtcgtg cttcatgatc
     4681 accgctccga cgttctccgg cgtctgtttg gtcggtcgtc ctgcgtggcg tcctgcggct
     4741 gcggctgctg ccatccctgc ctttgtgcgc tccttgaggg tggcgagttc catctctgcg
     4801 aaggcagcga ccatggtgag catcatcttc ccggtcgggg tgctgaagtc gaaaccttcg
     4861 cggaggctga tcaggctgac ctgccgcttc tccagctcct cgcagacctc catgaccgat
     4921 ctggcgttgc gtccgatgcg gtcgatcgcg accaccacga tgctgtcgcc tggtgcggca
     4981 aactccagga tggctttgaa cttgtcgcgc tgggtggcgg gtaccgttcc gctgatgccc
     5041 tcgtcggcgt ggaaggtggc ttgtgggtag cgatcgagga tcgctttctt ctgtccggct
     5101 actgtctgtt ctccggtgct gacgcgcccg tacccgaata ctctgctcat gctgtctcct
     5161 gttgtacaaa aaaggttgtt tggggatatt tgaccactta tgagcaaaaa gcaagcgcaa
     5221 aaaactgtaa acgcctagag ccacgggggc tgcgcagttt cgctcctttt tggacgtgat
     5281 caccccgcgc gaggcgaaag cctcgtgccc tctgcgttga tgtctgcgct gcgtcgtgac
     5341 ggcatgctgt ctgagcggcg tttcttgccg ctttgctgat aacttgctga gaaccaaacc
     5401 atgaccgaaa ttaagaaaga gagcgccgac ggcgcttggc tacgctctac ccttgccgaa
     5461 ttggcgggtg atgcgttccc tgtgtatgac ctcccgtgcc tgtcagtgga tccgaactcc
     5521 tccgctcaac ttgccgcgct tgacgatcgc cgggtggcgc gtgaaatgcg tcaggctgct
     5581 gccgacgtgg aaacgcgacg cattgacgcc gctcgcgtgg tggaagggct caagaccgag
     5641 gccgaacggc tgcgtggctt gatcgccgac ggcaaggcgg cgctgcgtgc tggcgagaca
     5701 gtgagccctg acgcgggtgt tgcgtcgttc ctgctgccgg acatcgaggc cgagctggtt
     5761 gttgctgagg ctgctgaggc tgatgtggcc cgggaacgtg acgggctgct gcaggatgcc
     5821 gatcgccgcg atgctgctgc tgcgctgacg ctgttcaact gggcgcactc cgttcgcgtg
     5881 cagcgtattg agctgcttct caaactggcg atggatgagg cgacgctgct tgccgagtcc
     5941 gacgcggggc gcagtttgta ccgtactgtt atcaatcctg atcgccgcct gaaccagctg
     6001 ttctccacgc agggtgcgat cgaaattctg cgccgcaatc gcggcatgga gggtgtgtaa
     6061 tgtctgcttt caaactcctg tgtgaccggc ttcgccatga ggcgacgctg gcaaaatcca
     6121 ccgacgctgg cgacgcgctg gcgcaggttc tggagacggg cggggcggtg cagacgttcg
     6181 cgccgatcgg ggctccggta gcgtctccga ccgaagctgt tcaccgcgcc cgtgctcagt
     6241 ccatccagga cgcggcgttt ctgaacgaga tgaaagcggc gggtgttgct ccggctgatc
     6301 tggtgatgct ggacggcgtg ttgacgtcct ctcgcgggat gccagacgcg gcgcgtgctc
     6361 cggcgttcgc tgctgtgcgt gctcaggtgc agcaacaggc gtctctctcg cccgcagcac
     6421 gggggataaa ttgggacgag taaagccgtt gccgcgtttc gtgctgcatg agccgtcgct
     6481 gtattcctgg ggcgtgttct gccgtggtag tgccagtgac gcgggcgtga aagctgcggg
     6541 cgacctgctc gatcagcacg cctttgctct cgccatcagc acgacgatcg ggggtggcca
     6601 ttgagtgagt ttgccgtctc gcgtctggtc atccctgagg tggtgatccc tgatggggtt
     6661 gttgctgcgc gaaaggccag ggggatcgcg caacgtgtcg cagctgctcg ccgtgctgcc
     6721 gagcgtcgtg ctgcgcctac gtggcgctgg gcgagacggg aaccgccccg gtcgacaggg
     6781 cgttgaaacg gtgagacggg gcgcgttatg cgcccttttt tttgtccctc gcctggcgtt
     6841 cctggcgtgc cttgcgccgg aatgccttga tctctgtcag gcgggctttg gcgttttcgg
     6901 cctcctccgg ggttgctggt tcggtggggt tgccgtccag gtcgtagcgc atgcactggt
     6961 cgatgaggga ttgctggtac gcgctcgtgc ccgtgatgat gcggaggatg cgacggacgg
     7021 cgttgcggtc tgtctctggc ggcattccca gctcctcgcc tcggttcagg atctgctcga
     7081 tgatgccttt tgccatcggg cggcggtcaa ctccccgggt cagtaagtca ggccagtgct
     7141 ctttaaggaa tgctttggtc acccggttgg ccagcgcctt tttggacttg ttggcgatcg
     7201 cgatgtcctg ctggcggcgg tcgttctcct cctgtctgcg gatcccctcg gcgacctgct
     7261 cgggcgtctt tttcggcttt gccgctgtgg gcttctgtgc gggcttctgc ggggctttcg
     7321 cttttggctg taccttcgcc ttgctggtag tccgttcggc tggctgtggc tcagatttcg
     7381 cggccttttc cggcgggacg tagaccgtgc ggttgcgtcg cttgatggtc aggatcgggc
     7441 gggtgggttc ggtcataaat ctctctcagg tctgttggtc tggatgtggt cggcaagggc
     7501 gatcagggtg gcggcgtcgc cgcggtcccg atcgatgcgc cggatgatgt cgttgatgta
     7561 gtcccagcgc ccgacggcgg caaggacgga aagggtgagg cggttgaacc ggctctctgg
     7621 tacgcgctcg agggtcaggg ggatgcgctt gcgggggcgt cccggactgc gccctgtccc
     7681 tggtcttttg agcggctgcg cccgctcctt ctgcggcaag acgacgggga tcttcgccag
     7741 gctgcgcgtc tcgttctgcg cgaactcggg caccgcgatg tactcgtctg tccatgtcct
     7801 gcctccgggt ctgtctgtcg cgctgtctcc gcacgccggg cagttgacgg tgatcgtgcg
     7861 gcggggcttc cccttcaccg gggctttccc gatgatgagc ggggcggttc tgcacctggc
     7921 gcaaaccgac gctgtccaga cggcattgtc tggcagggtg gcgttccggt actggtatgc
     7981 ggcgtcctgt gctgtctgga tggtcactgg ttgcatcacc ggggcgacgc tgagggctga
     8041 cgccgttggc gtgcagctgg ggcatgcgaa tgaaacgacg ccctggtgct cgtctgtgtc
     8101 ccatctgagc ggcttgccgt gctcggggca gaggtaggtg aaacctcccg gtgtggcggc
     8161 gaccgggcct ggtcggcgat cgtggaaccg tgcgccctgg ttggctcgca tggcgcgaat
     8221 gagcacctcg ccctggtgga tgccgtacca gacctgccag ccgtcgccga tgccgatcag
     8281 gaacggggag acgaacgggg gcgtgcgatt gactctcccg cgtgcgttga caccggtact
     8341 ggcttcccgc tcccggttcc agcaggagac gcacaaactc gacccgggct tgttgggggc
     8401 aattaggcgg tttgttgtgc ggctgcagcg agggcaaagg ttgcgggttt ctggcagggt
     8461 agtgacaggg gcttcgttgt cctgctcaat gccagaccag agcgcaaaac cgatcgggca
     8521 ccggcgacac tcgaggtggg ggttgtcgat gctcttgtcc tcgtggctgc gctggtacac
     8581 ctgtcggcat gcttcgacgc gaaggctgca cccgccgggg caggtcacga acaggacgcc
     8641 ggggagttgt tcggggaatt tttcagtcgc gcctacgcgc tcaagtgctg ccgggtttgg
     8701 ctgcgtcgat tcgttcgtgt cgttatcgtc gttcacacct tcctcccgcg tcgaaatggt
     8761 cgctaaccct ctacagcact ggatttgctg gttttttatt atcgcataat tagcgccaaa
     8821 atggcgttaa ctatgttacg cttgtcttac acttcctgat gagcagttgg gaaaacatgg
     8881 cccggatcgc tcgggtgctc agactatctc cgggggtctg ctctggtggc gcacttccac
     8941 ctggcgaaaa gcgaataagt gcgaagggct gctgtcctgc cgcattgcgg taagtcctca
     9001 agggcggctt tatcgacaat cgtcactcct gcccccgatg cccgttaagc tgaggggggc
     9061 acctatctgg tactcccaat gaaaaaacga atctataccg ctgacctcgc tgcgctgctg
     9121 aacgtgtcgc tgcgcacgct ctcgcggcta agttcgcggg ggatcgtgcc gactccggca
     9181 gggcgctccc tccgtggcgg gcattactgg ctggctggca atccagagat caaacgcttc
     9241 gttgccgacc agcgtcgtcc ggggagggct gctgatggcg ttcgataact ctcacctcga
     9301 ggatgctggt ctgttctggc gtgctgtctc ctctgccccg gctccggtgg atcgcgatga
     9361 gtggttgaag atggctcgcg gcatcgcgtg cttcgcgtct ctctgcggtg aggatgggct
     9421 ggcggctatc gaggcctggt ctacccgcca caatcgcggg gaggcaaaac accggagcca
     9481 gtaccggcat ttcctgaagc tgaaaggcat aaccaatccc gaggctatgg cgcaggtgtt
     9541 cctgcagttc tccggcgtcg aatggcgcac cggtcgcgct tctgtccagt ccgacgtcga
     9601 tcgcgctgaa cggctgcggt tgtcccttga ggccacagcg aaggcaaagg ctgaacgcga
     9661 caagaagggg gatgctgctg cgcgtgctgc ggtcaagctg ctggcgaagg gaggggatcc
     9721 tgcgtctacc ccatactggc gcaaaaagac cgctctggca gctgcgggtg gcgtccagct
     9781 gcccgccctc agcacagatc ttggtgttgg cgggatgttt cgatcgttca atccgcgtgc
     9841 ttactggtct gattgcctcc tgattccggt ttacaagttc gacgccgccc gtcagctgta
     9901 tgttgacggc ctggaggcca tctgcgggcg ttgctcggct gagggttccc gggtgatggg
     9961 cacgaagttc accccggcgg gtaccagaaa ggctggcggg ttctacccta tgccgggctt
    10021 catgaaggtg ctggacacct ccccccacgc gccctactcg cctctgttcg tctgcgaggg
    10081 cttcgctacg gcggtagctc tgcaccacct gagccgtggc aaggtggcgg tctgcgctgc
    10141 gttctcgatc gccgggttcg gcagctgcgt ggcgtcgctg cgtcggttct ggccagatgt
    10201 tgatttgatg ctggtaccgg atcacggcga agcgtaccgc ctggcgca
//
