LOCUS       NZ_OP242299             2345 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA207 plasmid pLAO94, complete sequence.
ACCESSION   NZ_OP242299
VERSION     NZ_OP242299.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363363
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600835.1-RS_2024_03_23
            Annotation Date              :: 03/23/2024 03:22:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 2
            CDSs (total)                 :: 2
            Genes (coding)               :: 2
            CDSs (with protein)          :: 2
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242299.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA207"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO94"
     gene            742..1710
                     /gene="mobV"
                     /locus_tag="PUP22_RS00005"
                     /old_locus_tag="KLOADLAJ_00001"
     CDS             742..1710
                     /gene="mobV"
                     /locus_tag="PUP22_RS00005"
                     /old_locus_tag="KLOADLAJ_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_025766223.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRVEREKQRQMRSRGMGGMER"
                     /name="mobV"
     gene            complement(1774..2205)
                     /locus_tag="PUP22_RS00010"
                     /old_locus_tag="KLOADLAJ_00002"
     CDS             complement(1774..2205)
                     /locus_tag="PUP22_RS00010"
                     /old_locus_tag="KLOADLAJ_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="WP_024183523.1"
                     /translation="MRRITQVDQSTGEELGGFVAVIRPKQKSSFQRHFTMNQAALITIA
                     NELNHDQMRVLMALLAELDYENYIQVAQIDIAEALTMQKTNVSRAVKNLIDFGIILEGP
                     KIGRSKTYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="lipoprotein"
     Typing          734..1707
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.462"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 catacatcgg caaaatcagg cgtttttctg cccgttcggt gcgaacgaat ttctatgtac
       61 gggaagtgga aattccgctt cctcgctcat tcgctcacta cgttcggtca tgagtctgcg
      121 gcgaaaagta cgattagtac ttgaggtgag tacgattagt acttgaggta agtacgatta
      181 gtacttgagg tgagtacgat tagtacttga ggtgagtacg attcttctct ctagcccttg
      241 gtgtgcgcgg gttcccgtat ctatatctat atattaaggg ggaaaataaa ggctgaaaaa
      301 cgccattttt aaacatcgaa aaacgagctt ttttgagtct ggagcgtacg gatttctatg
      361 tccggggagt ggaaattccg cttcctcgaa cggacagact cgcggcgatg ccgctcccta
      421 atccgttctt gcggcgagcg gttcctgctc tttcaaaaac gatattcatt gcctgatttc
      481 gtcaagggcg agcgtagcga gtgccattta cccttgacga ctcaccggat aaaacaggct
      541 tggcagtggt gggaggtgac gcggagcgac gccttccacc tgccagaccg ccgttgattt
      601 tgacctttgg aagcgcagct tcccgaacga ttttgcgaag caaagtctag tgagtacact
      661 atcatcttga tagtggcccg ccggaggtat cgcaatgcgc tcccccgcct ggctgaatga
      721 atgccggaat caggggggaa catggcgaac tacgcgataa tgcgctgcaa aaagctgacg
      781 ggaatgggaa gcgtggccag cgcgttgcag cactgctacc gagagcgtga gacgccgaac
      841 gctaacgctg aacggacacc agaaaactac tgctctgtgt ccaaatcaac ggatcaggca
      901 atggggcgtg tgcgggagct gctgccggaa aaacgccgta aagatgcggt actggccgtt
      961 gaatacgtta tgacagcaag ccctgagtgg tggaaagagg ccacaccacg gcagcaggcg
     1021 gagtttttcg cccgctctga gcaatggctg gagaagaaat acgggaagga tcgtgttgtg
     1081 gcggcggtgg ttcaccggga cgaggccacc ccgcacctgt ctgcgttcgt ggtgccattg
     1141 acgcaggatg gacggctgtc cgcgaaagag tttattggcg ggcggtcaaa gatgcgtgaa
     1201 gatcagagca cctacgctga atcagtaaaa aaactgggtc ttgagcgcgg catcgaggga
     1261 agccgcgcca cgcatcagac agttcagcac tactacgaat cgataaaccg tggcacccgt
     1321 agccaggtat cgatctcgcc ggaggcgctg gagccgcgtg tattgcggaa gggcattttc
     1381 accaaagacg tggaagatca ggcagccata gccaaacgtc taagccacgc agtgaacgat
     1441 ggctttgcag gaaccatcgc tatggcctct cagagcgccc agaacgcaaa aagagcgcgt
     1501 gagctacaga agactatgga tgcgcagcag aaacgcctac agagcgtcac agagcctttt
     1561 aaggggcttt ccagagagca gatgaccgaa atcctgatga tggctcagcg gtttaagcag
     1621 caaaaccagg agaaggagaa acagcagcgt gttgaacgtg agaaacagcg acagatgcga
     1681 tcgcgtggca tgggcggtat ggagcgttag ttttgatgcg atgcaggata gggctaacgc
     1741 cgcaaaatga cgccatttgc ggcattccag agatcaggtt ctgccgccct gtataacgct
     1801 gaggccgttt ttcagtgctt ttttgtggtt gctgacagta cctttccagc cgaactgggg
     1861 atttaggcgg tacgttttgc tccgcccaat cttcggtcct tccagtatga tcccgaaatc
     1921 aatcaggttt tttaccgcac gactgacatt tgttttttgc atagtcagcg cttctgcaat
     1981 atctatctgc gcgacctgga tgtagttctc gtaatccagc tctgcgagca aggccatcag
     2041 tacacgcatc tggtcatggt ttagctcatt ggcaatggta attagcgcgg cttgattcat
     2101 agtaaagtgc ctctggaatg atgacttttg tttgggacga atcactgcta caaaaccacc
     2161 taattcctcc cctgtacttt ggtctacctg cgtgattctc cgcatctaaa aatctccaat
     2221 ttcaggaaaa atagttatct ctcaagataa ctaagttatc tctagagata actattgtca
     2281 aaaactttcc gcttgtggcg cggcttccga gacatcccct tctgtatctt attactctcc
     2341 gtact
//
