LOCUS       NZ_OP242298             5279 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA207 plasmid pLAO93, complete sequence.
ACCESSION   NZ_OP242298
VERSION     NZ_OP242298.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363363
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Sequencing Technology             :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028600825.1-RS_2024_03_23
            Annotation Date                   :: 03/23/2024 03:30:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6
            CDSs (total)                      :: 6
            Genes (coding)                    :: 4
            CDSs (with protein)               :: 4
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242298.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA207"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO93"
     gene            join(3890..5279,1..182)
                     /locus_tag="PUP22_RS00005"
     CDS             join(3890..5279,1..182)
                     /locus_tag="PUP22_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017782005.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_039026336.1"
                     /translation="MTHIACHINTLQLPFVACTVPLTFTLPAAGCTLIGRNGIGKSLLL
                     ELLAGQRRADSGKIEWLTPISWLSQFAPAQDHDCAASVADLIGWGEPLRALERIEQGSC
                     AQDDFELLADRWNLRSDLADLLAEAGLRLDPHVPVSTLSGGQLTRLRLLALFARPAHFL
                     LLDEPDNHLDASGIDWLSQQLARHRGGYLLVSHDRRLLNQTSHILELSEQGLQSARLSF
                     DEFLAQQAEVQAARSTSLAQAVRSEKAAARSLQKVREQAARRASQGRRERGSQAKILLD
                     FKAEQAGKSLGRVLDRHERGHSAQVEARTEAAAALAAVQPLIMQWPEEIERKGAAQPLV
                     ITAASHVRWHGQRVDLVLQRGERCLLGGRNGCGKSSLLQMLRGVLEPETGSFRINGKMA
                     CLDQGLSLIARDLSPLANLLTVAPTLAESDARTRLAGIALRGDRALTPCHGLSGGERLK
                     LGLLMVLAQLPDLLLLDEPDNHLDHPSRQLLTQVLADYPGTLLLVSHDPDFVAGVGVGK
                     ELNLSE"
                     /name="ATP-binding cassette domain-containing protein"
     gene            <267..557
                     /locus_tag="PUP22_RS00010"
                     /old_locus_tag="FJNOHHKM_00001"
                     /pseudo=""
     CDS             <267..557
                     /locus_tag="PUP22_RS00010"
                     /old_locus_tag="FJNOHHKM_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004661993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /name="type II restriction endonuclease"
     gene            complement(1052..1273)
                     /locus_tag="PUP22_RS00015"
     CDS             complement(1052..1273)
                     /locus_tag="PUP22_RS00015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272954452.1"
                     /translation="MLHGASFEAMIERRLEEIKPKTPAQIKANGDSRNKTIVISALVVI
                     LAIIVIYIGAIYIIDSVTGKSSRPHLCP"
                     /name="hypothetical protein"
     gene            complement(1294..1488)
                     /locus_tag="PUP22_RS00020"
     CDS             complement(1294..1488)
                     /locus_tag="PUP22_RS00020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272954453.1"
                     /translation="MEYLKDINNNPSGLSIKKERHEPMEIITKKIKRLSTSCLGISNAS
                     TEPFIIQLVLTTVLKCEFW"
                     /name="hypothetical protein"
     gene            complement(1680..1862)
                     /locus_tag="PUP22_RS00025"
     CDS             complement(1680..1862)
                     /locus_tag="PUP22_RS00025"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272954454.1"
                     /translation="MAATNMSGTYNFNISLNRLDFTLNVSDSQFKEPEALSDFALKSKL
                     IVKTFLTKLRLSGLE"
                     /name="hypothetical protein"
     gene            complement(<3338..3463)
                     /locus_tag="PUP22_RS00030"
                     /old_locus_tag="FJNOHHKM_00004"
                     /pseudo=""
     CDS             complement(<3338..3463)
                     /locus_tag="PUP22_RS00030"
                     /old_locus_tag="FJNOHHKM_00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236504.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     Typing          complement(2241..2725)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.653"
                     /coverage="81.0"
                     /evalue="1.54e-148"
ORIGIN
        1 tactggcgca actgcccgac ctgctgctgc tcgatgaacc ggacaaccat ctggatcacc
       61 cctcgcgtca gttgctcact caggtcctgg cggactaccc aggcactctg ctattggttt
      121 ctcacgatcc cgacttcgtt gcaggggttg gggttggtaa agaattaaat ctttcagagt
      181 gaccgcaaga tgattgacct agatgccctt gtggcagggg gttagaggag ttgacggaaa
      241 tttcggcggt tccggcttac aaccccgaat accataaccc ggcgttcgac tcgctgaagc
      301 tgaccatgct tggtgtgaaa tcgacctgca aggaccgctg gcggcaggtt ctcgccgaag
      361 cagaccgcat tgatgacaag catctgctga cgctggaaac agcaatctcc acgcatcaga
      421 ccgacgaaat ggcagcaaag cggctgcaac tcgttctgcc gcgcagtctg caccagacct
      481 atacgccagc ccagcaggca tggttgatgg atgttgtcag tttcacggaa ctggtacgtg
      541 ctcgacagaa tgcgtgagtg ctgaacttcg gcttagcgta cgattttttc cgttttctga
      601 gccgaccctg gtgtgggcct ctgccatgcc tgattttgct caagttaatg cctcacggtc
      661 gcacagtaaa ctgtgcaccc gttcaccgtt atcgggggag ttatgcgccg gaaagcaggc
      721 tttcatgcca gcatccaccc caaatcgacc gccattcacc tgcgtgcata gttcatgcag
      781 ccgcgcagaa atcatcaggc gcgttttttc aatgatatgg acaggggaaa gatgccggaa
      841 ataggcagga gggcgcagaa atgccctgag agcctctcag gcggtgcacg caacggatgt
      901 tatggtaaat tgaagttaat ttcaccaatt tgtagttttg tgtttggggt aataagtgcc
      961 gccatatctt aatacagcag cgcttatttt ttatttggat gatttctctc cagaaagata
     1021 tccaaatacg ccactcctat tattgcggtt attacgggca taagtgagga cgtgaactct
     1081 tcccagtgac agaatcaatt atgtatattg ctccaatata tataactatt atagctaata
     1141 ttacaactaa agcagatatg acaatggttt tatttctgct gtcgccattt gctttgattt
     1201 gtgctggcgt cttaggtttt atctcttcca gtcttctttc tatcattgct tcgaaagaag
     1261 ccccatgcag catctatttg caaagcagac gagctaccag aattcacact tgagaactgt
     1321 ggtaaggacg agctggatga tgaacggttc tgtggaggca ttagatatcc ccaagcaact
     1381 tgttgacaac ctcttgattt tcttagttat tatttccatt ggctcatgtc tttctttttt
     1441 tattgataat cctgatggat tgttgtttat gtcttttaaa tactccatct gagactattc
     1501 ccttagtgtt tatctctgta ttgctaatcg agaaaacatt attaaaacta tatccgaatt
     1561 cctgagatat tttattgtat cggaaattaa aactcactta tgtttccaag atcttttttg
     1621 gagtattttt ttgctaagtg ctatagctgg aagtctttct aattcgaaat aattgccgat
     1681 tattccaatc cggataatct caatttcgtt aggaatgttt ttacaattag ttttgacttt
     1741 agtgcaaagt cacttaaagc ttctggctct ttaaattgag aatcggatac atttaatgtg
     1801 aaatctaatc tgttgagtga tatgttaaag ttatatgttc ccgacatatt tgttgctgcc
     1861 actctaggta tatctggtgg tacccctcat ctggaagtgg gataatttga ggcatcctat
     1921 caaataaatt gccagcggca tcattaatac tggaaaaaaa aatgatccgg tcttgttata
     1981 tgctgcttaa aagatagcca actggaaact catgacatgt accatttcaa ctcattttta
     2041 ttagtgtctt gactgaagta taaagcgaaa gtaatcttct tacgaagaaa aaaccgcccc
     2101 gcaggcggtt ttttagaggt tagaaagacc taggaaatct aactaaccgg aggtaacggc
     2161 ttagagggag cgcagtcacc aaacttgttc ttttagttta gcctttacag gcgcatcaca
     2221 tcaagactca attcctctaa tccgttacca gtggctgctg ccagtggcgc tttgtcgtgt
     2281 ctttccgggt tggacctgac gatagttacc gggacaggcg cagcagtcgg actgaacggg
     2341 ggttcgtgca cagtccagct tggagcgaac tgccttcccg gaaccgagtg tcaggcgtgg
     2401 aatgagaaac cgcggccata acagcgggaa tgacaccggt aaaccgaatg gcaggaacag
     2461 gagagcgcac gagggagcta ccagtgggga aacgcccgat ctttatagtc ctgtcgggtt
     2521 tcgccaccac tgatttgagc gtcagattct gtgatgcttt gtcagggggc gaagcctatg
     2581 gaaaaatggc tcgccgcggc ctttctgttc cctctctttc tccttccgct acccccggat
     2641 ctgtgggtaa cctgtgtgga ctgccggtac cgctcgccgc agccgaacgg ccgagcgtag
     2701 cgagtcagtg agcgaggaag cggaagattc agtgatgcct gattgggctc gtgtcgcgcc
     2761 gtacccttta ccgccggatt acacagtttc ctgcaatact ttccttccga tttacacatt
     2821 cgtctgcacg ataaacaacg gcagagtacg ggcaggatat gtaaagtagg cctacttacc
     2881 gctcgcctta ttcgcttaca gctcaaagga acatcgatgg ccaggtcccc cgatccacga
     2941 caacgtactg aggcttccgc gtgctggcgc gtcgctgagg tttcagcgca tggaccctgc
     3001 cggaacgccg ctgtacgaaa atattatcaa ggagcgggtt atttccgggg atatcacttt
     3061 acgaacaggg acgggcagaa attctggcgc gttaccagaa gcgtgcgaac ggccacattg
     3121 acacggacag gaaactaggt accatcgctt cctcaaaaaa tgcaacaggc gtggctccct
     3181 ctccggccag gcttttcctg tatttgctat ctgtttgcta cttgtttgct acttgtttgc
     3241 tacttgttta caattgtttg ctatctgttt gctatctgtt tgctacttgt ttgctaccct
     3301 ctgctgtgaa aagagtagca ggcacgttag cgatgtatac atcgccgcgc cgcaagggtt
     3361 ccccttcggt gaacacttta tccacctgga tctgttcgag ctgccgttct gggttctggt
     3421 cgaagctgct gacgcggacg taaccgatgc gctgaccgtg caaggtatcc tcctgaggga
     3481 aatgtgtcag gaagaaatct atgacccttg acggcgtatg tcaatcaatt cggaaggcaa
     3541 ctctattctg acgatttagc gccggatggt ctgacgccaa gttagggtat agcccagact
     3601 gacacttgac ttattgatac aacatgcaag tgagtgatac cggaacattt ggcatttttt
     3661 actatctgaa gtatgatttt actttcatca gtaaagagta tttcgccgat gcgcatttaa
     3721 cctgtccgca ccccactttt gattcttctc ccgccaggga gagcgggtgc gtctcaccgt
     3781 gacaggttga tgctgattcc tctgctgttt tcccactagt ggacacgtgt ccctgtcatt
     3841 tggtttagct cttggcctgc ggcttccgca ttggcctaca ggagttcaga tgacccatat
     3901 cgcatgtcat atcaacactt tgcagctgcc cttcgtcgcc tgtactgtgc cccttacctt
     3961 cactttgccc gctgcgggct gcaccctgat cggccgtaac ggcatcggca agagcctgct
     4021 gctggaactg ctggcgggcc agcgccgcgc ggattctggc aagatcgagt ggttgacccc
     4081 catcagctgg ttgtcccagt ttgcaccggc tcaagatcat gactgcgcag ccagtgtggc
     4141 cgaccttatc ggctggggcg agccgctacg ggcccttgag cgcatcgagc aggggagctg
     4201 cgcccaggac gatttcgagt tgctggctga tcgctggaac ctgcgcagcg atcttgccga
     4261 cctgctggca gaagcaggcc tcagactcga tccccatgtc ccggtcagca ccctgagcgg
     4321 tggccagctc acccgtttgc gcctgctggc gctgtttgcg aggcctgctc actttctgtt
     4381 gcttgatgag ccagacaacc acctcgatgc ctctggcatc gactggctaa gccagcaact
     4441 ggccagacat cgcggaggtt acctgctggt gagccacgac cggcgcctgc tcaatcaaac
     4501 cagtcatatt ctggagctga gcgagcaggg gttgcagagt gcccgcctct cgtttgacga
     4561 gtttctggca cagcaggccg aggtacaggc tgcccgcagc accagtttgg cgcaagcggt
     4621 gcgaagcgaa aaggcggcgg cgcgcagctt gcaaaaagtg cgtgaacagg cggccagacg
     4681 agccagtcag gggcggcgtg agcgcggctc ccaagccaaa atcctgcttg atttcaaggc
     4741 ggaacaggca ggcaaatccc ttggcagagt gctggaccgc cacgagcggg gccacagtgc
     4801 gcaagtggag gcccgcaccg aggcagcagc cgcgctggcg gcggtgcagc cgctcatcat
     4861 gcagtggccg gaggagatcg agcgcaaggg ggctgcccag ccgctggtga ttacggctgc
     4921 gtctcacgtg cgctggcatg gccagcgggt tgatctggtg ctgcaacggg gtgagcgctg
     4981 tttgctcggc ggtcgcaatg gctgcggcaa atcgagtctg ctgcagatgt tgcgtggggt
     5041 gctggagccc gagacgggca gctttcgcat caatggcaag atggcttgcc tcgatcaggg
     5101 gctcagcctg attgcgcggg atctcagccc gcttgccaac ctgttgacag tggctccgac
     5161 actggccgag agtgatgcga gaacacggct ggcgggcata gccctgcgcg gcgatcgtgc
     5221 tctgacgcct tgtcatggtc tgagtggggg ggagaggtta aaactgggtc tgctgatgg
//
