LOCUS       NZ_OP242296             4082 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA165 plasmid pLAO75, complete sequence.
ACCESSION   NZ_OP242296
VERSION     NZ_OP242296.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363356
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600805.1-RS_2024_03_23
            Annotation Date              :: 03/23/2024 03:22:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242296.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA165"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO75"
     gene            join(3982..4082,1..601)
                     /locus_tag="PUP30_RS00005"
                     /old_locus_tag="PFDAHMGA_00001"
     CDS             join(3982..4082,1..601)
                     /locus_tag="PUP30_RS00005"
                     /old_locus_tag="PFDAHMGA_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281397.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(857..3016)
                     /gene="mobQ"
                     /locus_tag="PUP30_RS00010"
                     /old_locus_tag="PFDAHMGA_00002"
     CDS             complement(857..3016)
                     /gene="mobQ"
                     /locus_tag="PUP30_RS00010"
                     /old_locus_tag="PFDAHMGA_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029344.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALVNAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            3195..3449
                     /gene="mobC"
                     /locus_tag="PUP30_RS00015"
                     /old_locus_tag="PFDAHMGA_00003"
     CDS             3195..3449
                     /gene="mobC"
                     /locus_tag="PUP30_RS00015"
                     /old_locus_tag="PFDAHMGA_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2659..3017)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.722"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3018..3194
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2..601
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(861..3103)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.733"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaagtctggg gctgatgcag aatctccaaa attgcaaaag acaatgacca ttacagctgc
       61 ggaatatttc gattcattcc ctgacatggg gcggaaaaat gccgaggttc agttacagga
      121 agcaattgat cgtttgtggg acaggtcaat cattctcaag gatgacgaga aacgtgagga
      181 gttccgctgg atccagtatc gggcacagta cgctaaaggc gaggccagag cacaaataac
      241 attttctgac gcagtaatgc cttatttgac acaactacaa gggcaattta ctagagttgt
      301 aatcaagaat atatctagat tatcaagaag ttacagtata aggatatacg aactcctcca
      361 gcagttccgc agtactggcg aacgtattat cgctctcgat gattttcgct caatgcttga
      421 tatagagcat aaatatcaaa catataaatc acttaaccag caattattaa gaccctgcat
      481 tgacgaactg aacaaaaaaa gcgacctggc tgtaacggtt gagacgatca aaaaggggcg
      541 cacggttgta gccctgcatt ttcgcttcaa ggaagacaaa caaatcaaga tgacgatcta
      601 aaatcgattt taaggcacct aaaacgctcc ctgctgcgtt ttttttgtgt tagtagtgcg
      661 aaggtactat tgaagtgaaa aattgcgtca aaagcgctct aatgcagcga aaaaagaacc
      721 cccatttttt gggggcggaa aaacaaaact atgcttgcat tatgtgtgtg cttcttgcat
      781 tcatccctgt cgatgaacat cgaagaacat cgacgaacat aacacgatgt taaacaaaac
      841 aaaatattgt tcacattcac tgtgaccaac cgcgatcatg tctcctctcg gtggcgtgtg
      901 ttcgtgcatc ttcctcgcgc cgccattcgt cccaggtacg attaaaaccc agcgagcgga
      961 acagatgatc cggctgtttt tctggtgtgt tgacggcagc aaaaagcggt gcctcacggg
     1021 ttttcagatg aaactccata agttccggcg caggtgatat gtggctgtct gcatcgtgat
     1081 ttacggcaac accataccct gcataccgta accccttatc ggtcagtgca ctgatgacca
     1141 gctggctgtt atcggctgag ggaacggcaa acgcgggata cagcgtacca ttaagccgga
     1201 caacaccgcc cagagagtca tccacgccca tgaccagcaa aagctgaagc tggcgggttt
     1261 cccggtcgac agaactcatt accggcatca gcgtcggtag cagacgtccc tgcgtaaaat
     1321 ccaccttcct tgctgccagt gatggcatgg ctggctggcg gtcttctcca agacagaaag
     1381 actccggcca tgtcttcggc tggcggagat tgcgggtata ttgcggccat gtctggcaaa
     1441 cctgcgtcag aagttgctca aaggtcgcag aggaataaac acgttgcagt ggtggatgtt
     1501 gaccgatttc gcttcggtca cagatataac cactcctgtc acgcattaaa tcaacacgct
     1561 caaagattct ggtgcggtat ttctccagct cgttccccca gagcgtgtat tctccgttcc
     1621 ggttgtgaag cagaaggtaa caggtcattt ccccgttacc aggcatgggg gcctcgccat
     1681 accccgtaac gactgcgctc agtacccctg aatccataac ggcattgtca tttttcctga
     1741 gctgttccgc tcgtcgctcc gcgcgactac gttctgtatc ctcctgctcc ttccggcgca
     1801 attcctcttc acgtttaagc aactggactc tgagagctgc aaggccggat gccccccact
     1861 gctgccactg tttttcaaat gccgtctgac tcgcgggaat gtcctgtttc tccaccttcc
     1921 ggtaatgctg tcgcaatttt tccatttgct ggctcagcga ctggattttt ccctgttcgc
     1981 tacggtattc cggtgccgcc cattgcatca ggggaatacg ggaaaaaccg gacgaaaacg
     2041 gcgatgattt tttcaggctg gcagcatggc tcatcgcttc ctttcgttcg gtgttcagct
     2101 cttcccagcg tctgcgcgtc gcctgcatat ctgccatcat cgacatggca ttactggtga
     2161 gtttccatgc cgcattactg tccttcggca acgcttcgat gatgtcacgt agtgtgtacg
     2221 aagttgcgtt gtcaggaagc acgatattca gaaggtcagc cacacggcgg gcaggctctg
     2281 tcagcccgct gtttctgagt tcggtacggg aaatctgtat ttccaggtta agtctgttaa
     2341 tcagacgatt ctgttcgccg cgttctgttt cgataccgcg tgtttccatc gcacaggcag
     2401 actttccgag gtgaatacca ggcgttttct caagcccctg tcgctcgtat gaacgatggt
     2461 ctatctcttc ctgataaccg gcgttcacca gtgccctgtt cgcatggtca gcccatgatg
     2521 cacgccacgt ctcggcatgt gtccggtcgt tccagtccct gacctttcca ccgaatccag
     2581 caggtccgac agctctcgtg gtcagcatga tgtgcgcatg gggatttgtt ttatccatgt
     2641 gatggaacgc cacatcagca atcatgccct gactgacaaa gttttctctg acaaaggcga
     2701 gaacggtttc ccgtgcggca tcctgcggta gttcgcgggg gatggcgagt tcaatttccc
     2761 tcgcaagttg tgagttcttc cggcgttcgg ccttctcaac ggcattccag agttcggcac
     2821 ggttcgtaca ccaggcaggg gctgtcgcgg gagtcagaat cacagcttca gccacaccgc
     2881 ttttgcgggt gtagtcgtga gtcagtccgg tacgctcatc aagcatcagt gaaccggaac
     2941 gataggcagc agaagcaacg gcgctgtagc cgctgtttct ggagatgatt ttcatgctga
     3001 gatgataaat cgccatttct gccgtaatgc ctttttgtgg ttttctttca gccccgcagg
     3061 gcgcacacaa tcgaaacgga gtggagattg tataagtgcg cacttcgtga ttttccgtgc
     3121 attatgccag ctcagttttt aaactaccag tgacgcgcta aaatgcgatt tttcatcgcg
     3181 cacggagtta catcatgagc agaacactcg aacagaagat tgctgatgct gaggccagat
     3241 tacagcgcct gaaggcgaag agcaggagtc tggacacagc gcagaaggtc attgtgggtg
     3301 cagcattgct ggcaaaggtc agaaagccgg aggaggtgca gttgcgtgcc tggttactcc
     3361 agttcctgaa ggcagaggtg acacgacagg ctgatgtgac gcgcatactg ccgctgatta
     3421 acgagctgga agcgctgcca ggacagtgat tgtatcgtcc gctcgccgca gtcccagcga
     3481 ccgagcgtta gcgagcgagt gggcgaggaa gcggaagtga atccgtcgcc tgattgagca
     3541 catacgcacg gtttttcggg aggtgatatg cgctgaaccc gcatgaatac tggatttcca
     3601 cagggttgga gggggtgtat tcggaacttg caattctgct tttttgcaac gcattgtttt
     3661 tattggtttt ttgttttcag tactccaagt tatccacagg ctcgtcgtta agtttttaga
     3721 tcttgttttt aagatcttgt ttttaatact tgttttagcg ccaaaattcc ttatagaaaa
     3781 caaaatgtta caatgctata ggtaatgtga tttaaggtaa aggtaatatg atttaaggtg
     3841 tttaggtggg ggaatttaag gtaaaggcaa tatgatttaa ggtaatattc acagaaaaag
     3901 ggatattaaa ataaaaaggc attgcctttc gttgagggta tgtttatctt ttattcatta
     3961 ccttttgggg agggatgctc tatgtcggat ctaattgcct ataaatccaa tgcgctggtt
     4021 gaagccagtt ataaattgac cctacaggaa cagcgatttt tgttactttg catcggtcga
     4081 tt
//
