LOCUS       NZ_OP242293             2029 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA189 plasmid pLAO85, complete sequence.
ACCESSION   NZ_OP242293
VERSION     NZ_OP242293.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363359
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600775.1-RS_2024_03_23
            Annotation Date              :: 03/23/2024 03:19:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 2
            CDSs (total)                 :: 2
            Genes (coding)               :: 2
            CDSs (with protein)          :: 2
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242293.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA189"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO85"
     gene            join(1982..2029,1..441)
                     /locus_tag="PUP04_RS00005"
                     /old_locus_tag="BOGPACHL_00001"
     CDS             join(1982..2029,1..441)
                     /locus_tag="PUP04_RS00005"
                     /old_locus_tag="BOGPACHL_00001"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272954450.1"
                     /translation="MVTLLPLLVTQDRLFSMSKDTQRITQINDQGEIVGGFVAVIRPKQ
                     KSSFQRHFTMNQDALKILAKELTGEQFKVLMLMLADLDYENFIQIAQADIADALEMQKT
                     HVSRAVRALLDVGVIFEGPKVGRSKTYRLNEQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="hypothetical protein"
     gene            complement(505..1473)
                     /gene="mobV"
                     /locus_tag="PUP04_RS00010"
                     /old_locus_tag="BOGPACHL_00002"
     CDS             complement(505..1473)
                     /gene="mobV"
                     /locus_tag="PUP04_RS00010"
                     /old_locus_tag="BOGPACHL_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_272954451.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKSIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     Typing          complement(509..1482)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.769"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 atgagcaagg acacccagag aatcacccag attaacgatc aaggtgagat tgttggcggc
       61 ttcgtcgcgg taatcagacc caagcaaaaa tcgtcatttc agaggcattt cactatgaac
      121 caagacgcat taaagatcct cgctaaggag ctaacgggcg agcaatttaa agttctaatg
      181 ctcatgctcg ctgatctgga ttacgagaat ttcatccaaa ttgcacaggc tgatatagcg
      241 gatgcattgg agatgcaaaa gacccacgta agccgcgctg ttcgtgctct tcttgatgta
      301 ggcgtgatct ttgaaggtcc gaaagtaggt agatccaaaa cctatcggtt gaacgagcaa
      361 ttcggctgga aaggaacagt aagcaatcac aaaaaagccc ttaaaaacgg cctcagcgtt
      421 atacagggcg gcagaacctg atctctggaa tgccgcaaat gacgtcactt tgcggcgtta
      481 gccctatcct gcatcgcatc aaaactaacg ctccatacca cccatgccac gcgatcgcgt
      541 ctgtcgctgt ttttcacgtt caatacgctg ctgtttttcc ttttcctggt tttgctgctt
      601 aaaccgctga gccatcatca ggatttcggt catctgctct ctggaaagcc ccttaaaagg
      661 ctctgtaacg ctctgtaggc gtttctgctg cgcatccata gtcttctgta gctcacgcgc
      721 tctttttgcg ttctgggcgc tctgagaggc catagcgatg gttcctgcaa agccatcgtt
      781 cactgcgtgg cttagacgtt tggctatggc tgcctgatct tccacgtctt tggtgaaaat
      841 gctcttccgc aatacacgcg gctccagcgc ctccggcgag atcgatacct ggctacgggt
      901 gccacggttt atcgattcgt agtagtgctg aactgtctga tgcgtggcgc ggcttccctc
      961 gatgccgcgc tcaagaccca gtttttttac tgattcagcg taggtgctct gatcttcacg
     1021 catctttgac cgcccgccaa taaactcttt cgcggacagc cgtccatcct gcgtcaatgg
     1081 caccacgaac gcagacaggt gcggggtggc ctcgtcccgg tgaaccaccg ccgccacaac
     1141 acgatccttc ccgtatttct tctccagcca ttgctcagag cgggcgaaaa actccgcctg
     1201 ctgccgtggt gtggcctctt tccaccactc agggcttgct gtcataacgt attcaacggc
     1261 cagtaccgca tctttacggc gtttttccgg cagcagctcc cgcacacgcc ccattgcctg
     1321 atccgttgat ttggacacag agcagtagtt ttctggtgtc cgttcagcgt tagcgttcgg
     1381 cgtctcacgc tctcggtagc agtgctgcaa cgcgctggcc acgcttccca ttcccgtcag
     1441 ctttttgcag cgcattatcg cgtagttcgc catgttcccc cctgattccg gcattcattc
     1501 agccaggcgg gggagcgcat tgcgatacct ccggcgggcc actatcagga tgatagtgta
     1561 ctcactagac tttgctacgc aaaatcgttc gggcagcaag ctgccagaag gtcaaaatca
     1621 acggcggtct ggcaggtgga aggcgtcgct ccgcgccccc ccccaccact gccaagcctg
     1681 ttttatccgg agtgtcgtca agggtaaatg gcactcgctt cgctcgccct tgacgaaatc
     1741 aggcaatgaa tatcgctttt gaaagagcag gaaccgctcg ccgcagactc atgaccgaac
     1801 gtagtgagcg aatgagcgag gaagcggaat ttccactccc cgtacataga aatccgtaca
     1861 ctccgtagct aaaaaacgtt ggtattcaga gctaaaccgc gcgcacgcgt tataagatac
     1921 agaaggagcg ataaaaaaac gtatgtatgg cgaggggtta catttgacat agttaccaat
     1981 aatggtaaca ctgttaccat tattggtaac tcaagatagg ttattttcg
//
