LOCUS       NZ_OP242289            22368 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA170 plasmid pLAO78, complete sequence.
ACCESSION   NZ_OP242289
VERSION     NZ_OP242289.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363357
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22368)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22368)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Sequencing Technology             :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028600735.1-RS_2024_03_23
            Annotation Date                   :: 03/23/2024 03:27:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 23
            CDSs (total)                      :: 23
            Genes (coding)                    :: 18
            CDSs (with protein)               :: 18
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242289.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..22368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA170"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO78"
     gene            join(20785..22368,1..1383)
                     /locus_tag="PUP24_RS00005"
     CDS             join(20785..22368,1..1383)
                     /locus_tag="PUP24_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="WP_001138014.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRGD
                     ANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRREHA
                     QELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALSVID
                     RACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKPNSRH
                     MLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYATLAAV
                     VLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIGQALLEA
                     KESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRYTPALLEV
                     LELRAAPAAQGVLAAVQTLREMNADNLRKVPADAPTAFIKPRWKPLVITPEGLDRKFYE
                     ICALSELKNALRSGDIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAINPNSDQYLE
                     ERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALIDQTSQLLPRI
                     KITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLTKMAESSPGLTY
                     AKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQRFRAGGRGESTG
                     HVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYHESDLRIEEHYTD
                     TAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRPLIGGTLNIKHVRAH
                     WDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVEL
                     RRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYL
                     ERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSRRLEDGKFRPLRMPGKP"
                     /name="Tn3-like element TnAs1 family transposase"
     gene            1425..1667
                     /locus_tag="PUP24_RS00010"
     CDS             1425..1667
                     /locus_tag="PUP24_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032140899.1"
                     /translation="MRKAEREDGRERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="hypothetical protein"
     gene            complement(1699..2349)
                     /gene="tetR(A)"
                     /locus_tag="PUP24_RS00015"
                     /old_locus_tag="KOCFHKGB_00002"
     CDS             complement(1699..2349)
                     /gene="tetR(A)"
                     /locus_tag="PUP24_RS00015"
                     /old_locus_tag="KOCFHKGB_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_001493764.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEADPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            2455..3654
                     /gene="tet(A)"
                     /locus_tag="PUP24_RS00020"
                     /old_locus_tag="KOCFHKGB_00003"
     ARG             2456..3651
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="99.75"
                     /coverage="100.0"
     gene            complement(3686..>4036)
                     /locus_tag="PUP24_RS00025"
                     /pseudo=""
     CDS             complement(3686..>4036)
                     /locus_tag="PUP24_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /name="EamA family transporter"
     gene            4895..5122
                     /locus_tag="PUP24_RS00115"
     CDS             4895..5122
                     /locus_tag="PUP24_RS00115"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_326465083.1"
                     /translation="MDRERCRKRRGRGRNALRASQGVRARDVMVNTYFILMEIILISGS
                     CLCGAVKFTIKNQPENFIDATVPYAEDSLE"
                     /name="hypothetical protein"
     gene            5140..5457
                     /locus_tag="PUP24_RS00030"
     CDS             5140..5457
                     /locus_tag="PUP24_RS00030"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde-activating
                     enzyme"
                     /protein_id="WP_240761615.1"
                     /translation="MVKVEDFDWQTSEELISSWKKESGYRNDFCKKCGSTVPNILRGQP
                     YVWLPLGLLNENIKADCCGDFCTDDSMMWDCVRSANSNKKAVSSLFDLLVMLNVEQAKL
                     E"
                     /name="glutathione-dependent formaldehyde-activating
                     enzyme"
     gene            6255..6446
                     /locus_tag="PUP24_RS00035"
                     /old_locus_tag="KOCFHKGB_00005"
     CDS             6255..6446
                     /locus_tag="PUP24_RS00035"
                     /old_locus_tag="KOCFHKGB_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6668..7036
                     /locus_tag="PUP24_RS00040"
     CDS             6668..7036
                     /locus_tag="PUP24_RS00040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272954397.1"
                     /translation="MILAKKRPFPGKGHARMTLWVQRLHINYKIASAFTPERGCTTELH
                     QKYIKITSLLRHFYIIFASDLTSELFVHPPPLPGPPARAENGFSGCAGNASRPRNKLRI
                     RSPLSGWFVVAFRAFIGF"
                     /name="hypothetical protein"
     gene            complement(7270..7548)
                     /locus_tag="PUP24_RS00045"
                     /old_locus_tag="KOCFHKGB_00006"
     CDS             complement(7270..7548)
                     /locus_tag="PUP24_RS00045"
                     /old_locus_tag="KOCFHKGB_00006"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="WP_272954398.1"
                     /translation="MLLRVWSTELALTNQRVIAKVGLIRRNTVELRIDKVESLGIHQGI
                     LGRIFNYGSIVIKGTGGTNTPIPNIKAPMQFRSIVNNHIEEMNSKQQ"
                     /name="PH domain-containing protein"
     gene            complement(7711..>8001)
                     /locus_tag="PUP24_RS00050"
                     /old_locus_tag="KOCFHKGB_00007"
                     /pseudo=""
     CDS             complement(7711..>8001)
                     /locus_tag="PUP24_RS00050"
                     /old_locus_tag="KOCFHKGB_00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004661993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /name="type II restriction endonuclease"
     gene            complement(8086..9657)
                     /locus_tag="PUP24_RS00055"
                     /old_locus_tag="KOCFHKGB_00008"
     CDS             complement(8086..9657)
                     /locus_tag="PUP24_RS00055"
                     /old_locus_tag="KOCFHKGB_00008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017782005.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_039026336.1"
                     /translation="MTHIACHINTLQLPFVACTVPLTFTLPAAGCTLIGRNGIGKSLLL
                     ELLAGQRRADSGKIEWLTPISWLSQFAPAQDHDCAASVADLIGWGEPLRALERIEQGSC
                     AQDDFELLADRWNLRSDLADLLAEAGLRLDPHVPVSTLSGGQLTRLRLLALFARPAHFL
                     LLDEPDNHLDASGIDWLSQQLARHRGGYLLVSHDRRLLNQTSHILELSEQGLQSARLSF
                     DEFLAQQAEVQAARSTSLAQAVRSEKAAARSLQKVREQAARRASQGRRERGSQAKILLD
                     FKAEQAGKSLGRVLDRHERGHSAQVEARTEAAAALAAVQPLIMQWPEEIERKGAAQPLV
                     ITAASHVRWHGQRVDLVLQRGERCLLGGRNGCGKSSLLQMLRGVLEPETGSFRINGKMA
                     CLDQGLSLIARDLSPLANLLTVAPTLAESDARTRLAGIALRGDRALTPCHGLSGGERLK
                     LGLLMVLAQLPDLLLLDEPDNHLDHPSRQLLTQVLADYPGTLLLVSHDPDFVAGVGVGK
                     ELNLSE"
                     /name="ATP-binding cassette domain-containing protein"
     gene            10084..10644
                     /locus_tag="PUP24_RS00060"
                     /old_locus_tag="KOCFHKGB_00009"
     CDS             10084..10644
                     /locus_tag="PUP24_RS00060"
                     /old_locus_tag="KOCFHKGB_00009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636773.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_039022047.1"
                     /translation="MQGQRIGYIRVSSFDQNPERQLEHIQVDKVFIDKASGKDTQRPQL
                     DVLLSFVREGDTVVVHSMDRLARNLDDLRRLVQGLTQRRVCIEFVKEHLTFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSDPEIAEVRRRVIAGEK
                     KATLAREFGISRETLYQYLRVPE"
                     /name="recombinase family protein"
     gene            10648..13680
                     /locus_tag="PUP24_RS00065"
                     /old_locus_tag="KOCFHKGB_00010"
     CDS             10648..13680
                     /locus_tag="PUP24_RS00065"
                     /old_locus_tag="KOCFHKGB_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006087332.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_039022048.1"
                     /translation="MAIAKSERLTVLSDAEQEALYGLPDFDDAQRLEYLALTENELALA
                     SSRPSIHAQVYCILQIGYFKAKNDFFRFYWRDVEDDCAFVLSRYFQGEAFETKQISKHE
                     HYSQRGKIAALFGYQPWASSYLPSLEHQASLIVRRDVTPNFVAAELIVWLNEQKIIRPG
                     YTTLQALVSEVLSAERRRLGALLAQVLDEPAKTSLSQLLVRDDTLSQLAALKQDAKDFG
                     WRQMARERDKRTLLAPLHGIAKGLLPKLGISQQNLLYYASLANFYTVHDLRNLKPEQTQ
                     LYLLCYAWVRYRQLSDNLVDAMAFHMHQLDHENRAGARESFAEEQVKRHQETPQVGRLL
                     SLYVDDSVADPTPFGEVRQRAYKIMAKDVLQNTAQRMSVKPLNKLTLHWQAVDGQAKRI
                     SRHLRPLFVALEFAATDPDSQWLAALTWVKSVFAKQLRLSQRPLAECPAATLPKRLRPY
                     LLSFDADGEPTGLHADRYEFWLYRQVTKRFQSGELYLDDSLQHRHFSDELVSMEEHAQE
                     LAQMDIPFLRQPVETQLDALASELHKQWRAFNRELKQGKLTHLEYDKDTQKLTWRKPKG
                     ENQKAREQTFYDQLPYCDVADVFRFVNNQCQFLSALTPLQPRYAKKVADTDSLIAVIIA
                     QAMNHGNLVMARTSDIPYHVLESAYQQYLRQASLHEANDGISNAIAALPIFPYYSFDLD
                     VLYGAVDGQKFSVERPTVKARYSRKYFGRGKGVVAYTLLCNHIPLNGYLIGAHDYEAHH
                     VFDIWYRNTSDIVPEVITGDMHSINKANFAILHWFGRRFEPRYTDLNAQLQALYCVGDP
                     ARYEKCLIQPVGQIDRQLIVSEKANIDRIIATLGLKEMTQGTLIRKLCTYTAPNPTRRA
                     IFEFDKLVRSIYTLRYLRDPQLERNVHRSQNRIESYHQLRSSIAQVGGKKELTGRTDIE
                     IEICNQCARLIANAVIYYNSAILSRLLTKCEATGNAKALAALTQISPAAWRHILLNGHY
                     TFQSDGKVIDLDALVAGVELA"
                     /name="Tn3 family transposase"
     gene            complement(13731..14063)
                     /gene="qac"
                     /locus_tag="PUP24_RS00070"
                     /old_locus_tag="KOCFHKGB_00011"
     CDS             complement(13731..14063)
                     /gene="qac"
                     /locus_tag="PUP24_RS00070"
                     /old_locus_tag="KOCFHKGB_00011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacL"
                     /protein_id="WP_000800531.1"
                     /translation="MKNWLFLAIAIFGEVVATSALKSSHGFTKLVPSVVVVAGYGLAFY
                     FLSLALKSIPVGIAYAVWAGLGIVLVAAIAWIFHGQKLDLWAFVGMGLIVSGVAVLNLL
                     SKVSAH"
                     /name="qac"
     gene            complement(14186..15024)
                     /gene="aadA1"
                     /locus_tag="PUP24_RS00075"
                     /old_locus_tag="KOCFHKGB_00012"
                     /pseudo=""
     ARG             complement(14237..15024)
                     /name="aadA1"
                     /gene="aadA1"
                     /gene_name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(15117..16376)
                     /locus_tag="PUP24_RS00080"
                     /old_locus_tag="KOCFHKGB_00013"
     CDS             complement(15117..16376)
                     /locus_tag="PUP24_RS00080"
                     /old_locus_tag="KOCFHKGB_00013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001256773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol efflux MFS transporter CmlA1"
                     /protein_id="WP_000095725.1"
                     /translation="MSSKNFSWRYSLAATVLLLSPFDLLASLGMDMYLPAVPFMPNALG
                     TTASTIQLTLTTYLVMIGAGQLLFGPLSDRLGRRPVLLGGGLAYVVASMGLALTSSAEV
                     FLGLRILQACGASACLVSTFATVRDIYAGREESNVIYGILGSMLAMVPAVGPLLGALVD
                     MWLGWRAIFAFLGLGMIAASAAAWRFWPETRVQRVAGLQWSQLLLPVKCLNFWLYTLCY
                     AAGMGSFFVFFSIAPGLMMGRQGVSQLGFSLLFATVAIAMVFTARFMGRVIPKWGSPSV
                     LRMGMGCLIAGAVLLAITEIWALQSVLGFIAPMWLVGIGVATAVSVAPNGALRGFDHVA
                     GTVTAVYFCLGGVLLGSIGTLIISLLPRNTAWPVVVYCLTLATVVLGLSCVSRVKGSRG
                     QGEHDVVALQSAESTSNPNR"
                     /name="chloramphenicol efflux MFS transporter CmlA1"
     gene            complement(16591..17429)
                     /locus_tag="PUP24_RS00085"
                     /old_locus_tag="KOCFHKGB_00014"
                     /pseudo=""
     CDS             complement(16591..17429)
                     /locus_tag="PUP24_RS00085"
                     /old_locus_tag="KOCFHKGB_00014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /name="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
     gene            complement(17435..17725)
                     /locus_tag="PUP24_RS00090"
     CDS             complement(17435..17725)
                     /locus_tag="PUP24_RS00090"
                     /inference="COORDINATES: protein motif:HMM:NF017990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1010 domain-containing protein"
                     /protein_id="WP_001336345.1"
                     /translation="MFIQTAFSFSGVIQCLFCLFSGLRLHGLRRFSVFLASSPCVASAS
                     SYRFCSAVPPRWRSVFSRLAPVAKFKLSVLASGSNISVKPTRILRSAYLAR"
                     /name="DUF1010 domain-containing protein"
     gene            complement(17837..18334)
                     /gene="dfrA12"
                     /locus_tag="PUP24_RS00095"
                     /old_locus_tag="KOCFHKGB_00015"
     ARG             complement(17841..18334)
                     /name="dfrA12"
                     /gene="dfrA12"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA12"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            18479..19492
                     /gene="intI1"
                     /locus_tag="PUP24_RS00100"
                     /old_locus_tag="KOCFHKGB_00016"
     CDS             18479..19492
                     /gene="intI1"
                     /locus_tag="PUP24_RS00100"
                     /old_locus_tag="KOCFHKGB_00016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="WP_000845039.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLPTEQAYVHWVRAFI
                     RFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQEI
                     GRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDFDHG
                     TIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALERKYPR
                     AGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATPHTLRH
                     SFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLTSER"
                     /name="intI1"
     gene            <19695..20045
                     /locus_tag="PUP24_RS00105"
                     /old_locus_tag="KOCFHKGB_00017"
                     /pseudo=""
     CDS             <19695..20045
                     /locus_tag="PUP24_RS00105"
                     /old_locus_tag="KOCFHKGB_00017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /name="DUF3330 domain-containing protein"
     gene            20221..20781
                     /locus_tag="PUP24_RS00110"
                     /old_locus_tag="KOCFHKGB_00018"
     CDS             20221..20781
                     /locus_tag="PUP24_RS00110"
                     /old_locus_tag="KOCFHKGB_00018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001161490.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQL
                     EALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATAGEP
                     KAQLAREFNISRETLYQYLRTDD"
                     /name="recombinase family protein"
     ARG             complement(13735..14063)
                     /name="qacL"
                     /gene="qacL"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacL"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(16642..17429)
                     /name="aadA2"
                     /gene="aadA2"
                     /gene_name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA2"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(15121..16376)
                     /name="cmlA1"
                     /gene="cmlA1"
                     /gene_name="chloramphenicol efflux MFS transporter CmlA1"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(16639..17417)
                     /name="aadA2"
                     /gene="aadA2"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(14234..15012)
                     /name="aadA"
                     /gene="aadA"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.61"
                     /coverage="98.48"
     Typing          complement(5757..6380)
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.448"
                     /coverage="99.0"
                     /evalue="7.140000000000001e-167"
ORIGIN
        1 accgtcaccc gcctggccaa ggacaacgag ctgcccgatg ccatcctcac cgagtcaggg
       61 ctgaaaatca ccccgctgga tgcggcggtg ccggatcggg cgcaggcgct gatcgaccaa
      121 accagccagt tactgccgcg catcaagatc accgaactgc tgatggacgt ggacgactgg
      181 acgggcttca gccgccactt cacccacttg aaggacgggg ccgaggccaa agacaggacg
      241 ttgctgctgt ccgcaatcct cggtgatgcg atcaacctcg ggctgaccaa gatggccgag
      301 tcgagccccg gcctgaccta cgccaagctg tcctggctgc aagcctggca catccgcgac
      361 gaaacctatt cggcggcctt ggccgagctg gtcaaccacc agtatcgcca cgcctttgcc
      421 gcccactggg gcgacggcac gacctcatcc tccgatggcc agcgcttccg cgcgggtggc
      481 cggggcgaga gcaccgggca cgtcaacccg aagtacggta gcgagccggg acggctgttc
      541 tatacccata tctccgacca gtacgcgccg ttcagcaccc gcgtggtgaa tgtcggcgtc
      601 cgcgattcca cctatgtgct cgacggcctg ctgtaccacg agtccgacct gcggatcgag
      661 gagcactaca ccgacacggc cggcttcacc gatcacgtct ttgccctgat gcacctgcta
      721 ggcttccgct tcgcgccgcg catccgcgac ctcggcgaaa ccaagctgta cgtgccgcag
      781 ggcgtgcaag cctacccgac gttgcgcccg ctgatcggcg gcaccctgaa catcaagcac
      841 gtgcgtgccc actgggacga catcctgcgc ctggccagct cgatcaagca gggcaccgtc
      901 accgcctcgc tgatgctgcg caagctcggc agctacccgc gccagaacgg actggccgtg
      961 gccctgcgcg agctgggccg gatcgagcgc acgctgttca tcctggactg gctgcaaagt
     1021 gttgaactgc gccgccgcgt gcatgccggc ctgaacaaag gtgaggcgcg caactcgctg
     1081 gccagggcgg tgttcttcaa ccgccttggg gaaatcaggg atcggagctt cgagcagcag
     1141 cgctaccggg ccagcggcct caacctggtg acggcggcta tcgtgctgtg gaacacggtg
     1201 tacctggaac gcgccaccca ggggttggtc gaggccggca agccggtgga cggcgagctg
     1261 ctgcaattcc tgtcgccgct gggctgggag cacatcaacc taaccggcga ttacgtctgg
     1321 cggcagagcc gcagactgga agacgggaag tttcggccct tacggatgcc cggaaaacct
     1381 tagcgtacga ttttttccga attctgcggg ctcccctatc tcatctgcgc aaggcagaac
     1441 gtgaagacgg ccgcgagcgc caggcgcacg aggccggcgc gcggacccgc gccacggccc
     1501 acgagcggac gccgcagcag gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1561 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1621 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1681 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1741 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1801 cggatcggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1861 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1921 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1981 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     2041 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     2101 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     2161 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     2221 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     2281 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     2341 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2401 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2461 cccaacagac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2521 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2581 gcccactatg gcattctgct ggcgctgtat gcgttgatgc aatttgcctg cgcacctgtg
     2641 ctgggcgcgc tgtcggatcg tttcgggcgg cggccggtct tgctcgtctc gctggccggc
     2701 gctgctgtcg actacgccat catggcgacg acgcctttcc tttgggttct ctatatcggg
     2761 cggatcgtgg ccggcatcac cggggcgact ggggcggtag ccggcgctta tattgccgat
     2821 atcactgatg gcgatgagcg cgcgcggcac ttcggcttca tgagcgcctg tttcgggttc
     2881 gggatggtcg cgggacctgt gctcggtggg ctgatgggcg gtttctcccc ccacgctccg
     2941 ttcttcgccg cggcagcctt gaacggcctc aatttcctga cgggctgttt ccttttgccg
     3001 gagtcgcaca aaggcgaacg ccggccgtta cgccgggagg ctctcaaccc gctcgcttcg
     3061 ttccggtggg cccggggcat gaccgtcgtc gccgccctga tggcggtctt cttcatcatg
     3121 caacttgtcg gacaggtgcc ggccgcgctt tgggtcattt tcggcgagga tcgctttcac
     3181 tgggacgcga ccacgatcgg catttcgctt gccgcatttg gcattctgca ttcactcgcc
     3241 caggcaatga tcaccggccc tgtagccgcc cggctcggcg aaaggcgggc actcatgctc
     3301 ggaatgattg ccgacggcac aggctacatc ctgcttgcct tcgcgacacg gggatggatg
     3361 gcgttcccga tcatggtcct gcttgcttcg ggtggcatcg gaatgccggc gctgcaagca
     3421 atgttgtcca ggcaggtgga tgaggaacgt caggggcagc tgcaaggctc actggcggcg
     3481 ctcaccagcc tgacctcgat cgtcggaccc ctcctcttca cggcgatcta tgcggcttct
     3541 ataacaacgt ggaacgggtg ggcatggatt gcaggcgctg ccctctactt gctctgcctg
     3601 ccggcgctgc gtcgcgggct ttggagcggc gcagggcaac gagccgatcg ctgatcgtgg
     3661 aaacgatagg cctatgccat gcgggtcaag gcgacttccg gcaagctata cgcgccctag
     3721 gagtgcggtt ggaacgttgg cccagccaga tactcccgat cacgagcagg acgccgatga
     3781 tttgaagcgc actcagcgtc tgatccaaga acaaccatcc tagcaacacg gcggtccccg
     3841 ggctgagaaa gcccagtaag gaaacaactg taggttcgag tcgcgagatc ccccggaacc
     3901 aaaggaagta ggttaaaccc gctccgatca ggccgagcca cgccaggccg agaacattgg
     3961 ttcctgtagg catcgggatt ggcggatcaa acactaaagc tactggaacg agcagaagtc
     4021 ctccggccgc cagttggggt cagtttggat atagagaatt attgtacggt aagcctgttt
     4081 tttgaacaga cttacctata gcaatagggt gtactcccgc gctatacggc attttcagag
     4141 gtttgacctt aatcggataa cctaaatgcg atactgcaag agcgacaact taaaagcgat
     4201 aaccactgac gcaaaatcaa ccccttacga accaagattg gttaaataat gcgcttaacg
     4261 tacaaaaaat tccgatctcc aaactgaccc cttcttcgcc tcttatgttt ctcgaatgaa
     4321 tcgatgggga caggaaatgt tttgtggcca ccgccgcttc gccggcacca caaaactgtc
     4381 ggacaagatg tgtttttgga gtaccgccgg caccaaaaac atttcatcag gcgcagcctg
     4441 ataccctgaa tttcgtacgc acgaaatttt gcttttgttc agatcgaggg caggattcgc
     4501 aaagtgctgg cgtttgtacc caccggaagc tgccgcgcct ggcggcttgg ccaacgcctc
     4561 acggccgcac agtcaactgt gcacccgttc accgttacca gatcccctga ttccggccgg
     4621 gcctgcctgc actggccaaa gccttaaaac tgcgcaatcc gcagcgcggg ggcagttgat
     4681 aaccactttt tactgtcata cttatttgcc ggctctctga tcggtaccgg ctgactgttt
     4741 gtgcaaattt tgtcggacat cctgaccctc gatgtccgtt ttttttgttc aaaattcgcc
     4801 tttagtgcca gcattcgtcc gaaaccggcc gccattcact tccgtgcata gccatgcagg
     4861 cgcgcagaaa tcatcaggcg cgttttttca cgatatggac agggaaaggt gccggaaacg
     4921 cagaggacgc ggcagaaacg cgctgagggc ctctcagggg gtgcgggcaa gagatgttat
     4981 ggtaaatacg tattttattt tgatggagat tattttgatt agtgggtcat gtttgtgtgg
     5041 tgctgttaaa ttcacgataa aaaatcagcc tgaaaatttt atagatgcca ctgttcccta
     5101 tgccgaagac agtctggagt aggtcataac ttagctgcca tagtgaaagt tgaggatttt
     5161 gactggcaaa cctcagagga attgatttct tcatggaaaa aggaaagtgg ttaccgtaac
     5221 gatttctgca aaaaatgtgg ttcgactgtt ccaaatattt tgagggggca gccttatgtg
     5281 tggctaccac ttgggctact taacgagaat attaaagctg actgctgtgg tgatttttgt
     5341 acagacgatt cgatgatgtg ggactgtgtg cgctcagcga atagtaacaa aaaagcggta
     5401 agtagcctgt tcgacttatt ggttatgctc aatgttgagc aggctaaact tgaatagata
     5461 gcgagcacgc cttagggatg ttgcttaatg gattgtattg gaaagatgca ctgatggata
     5521 gttatcttga cctgataagg ggttaccgcg tagattagtt gtcgcaaacg aaaaaaccgc
     5581 cttgcagggc ggtttttcga aggtactcag agctagcaac tctttgaacc gaggtaactg
     5641 gcttggagga gcgcagtcac caaaacttgt cctttcagtt tagccttaac cggcacatga
     5701 cttcaagtcc atctcctcta aatcagttac cagtggctgc tgccagtggc gctttgtcgt
     5761 gtctttccgg gttggactca agatgatagt taccggacaa ggcgcagcgg tcggactgaa
     5821 cggggggttc gtgcatacag tccagcttgg agcgaactgc cttcccggaa ctgagtgtca
     5881 ggcgtggaat gagataaacg cggccataac agcggaatga caccggtaaa ccgaaaggca
     5941 ggaacaggag agcgcacgag ggagctacca gggggaaacg cctgggatct ttatagtcct
     6001 gtcgggtttc gccgccactg atttgagcgt cagattttgt gatgcttgtc aggggggcgg
     6061 agcctatgga aaaacgcccc gccggggcgt ggccttttcg cccttgttct tagctttcgt
     6121 tatgcttacc gttacgttat ctgcttatcc tccctccgct cgccgcagac tcatgaccga
     6181 acggagtgag cgaatgagcg aggaagcgga aaaccatcag aacgtacagt aagcactttc
     6241 gcgccggggc cttcatgaac aaacaacaaa agacagccgt taacatggct aaattcatcc
     6301 aggatcagtc cctgcttctt ctggacaggc ttaatgagct ggatctggat catgaggctg
     6361 atttctgcga gaaactccat gaggatgcgg aacgccttta ccgaagcctg tcagcgcgcc
     6421 tgaatgagca gcaggacggt gcgtaatggc tccgttctgg gtattaccgg agtaataccg
     6481 gcatcgcctt aattttcatt gtgttagcgc ttaacattat taccccggta atacccagaa
     6541 tccctgtatt ttttcttttt tatttatttt tcatgaagtt gcgatgaaaa tcgcagctgc
     6601 gttaggtgta tgtcattttt tagtatataa aacgaccacc cccgagtgct ggcgccgcag
     6661 gttaaggatg attctggcta aaaagcgtcc ttttccggga aagggtcatg ctcgtatgac
     6721 cctatgggtt cagcgactac acataaacta caaaattgca tctgctttta ccccagaacg
     6781 cgggtgtaca acagaattac atcaaaagta catcaaaatt acatcacttt tacgtcactt
     6841 ttacatcatt tttgcgtcag atctgacctc tgaactattc gtacatccgc cccctcttcc
     6901 tgggcctcct gcacgtgctg aaaatggttt ttccggctgt gccggcaacg cctcacggcc
     6961 acgcaataaa ttgcgcatcc gttcaccgtt atcggggtgg ttcgtggtgg ctttcagggc
     7021 gttcatcgga ttttagccgc taaaatcccg gccaggtatg tccgtgcata gccatgcagt
     7081 cgcgccagaa tcatcagggg cgttttttct cgatatgggc acagaaaggt gtgtgtttca
     7141 ggccggaggc ctcagaaacg ccctgagggc ctctgaggcg gtgcgggcaa cggatgttat
     7201 ggtaaatcca gctgattggc aggtaaaaaa aaagccctac ggggcttttt ttgtaatgct
     7261 acgaggaagc tactgctgct tagaattcat ttcctcgata tgattgttca caatcgatct
     7321 gaattgcatt ggcgccttga tgtttggaat tggcgtgttg gttccgccag tgcctttaat
     7381 cacgatagag ccatagttaa agattcgtcc cagtatccct tgatgtatgc caaggctttc
     7441 aactttatca atacggagtt ccaccgtatt gcgtcggatc aaacccactt ttgcgatgac
     7501 tctttggtta gttaatgcaa gctccgttga ccaaactcta aggagcactg ggattattat
     7561 aagtaaccct aagccgtaaa aaggcagcaa caagattccg ataattactg ggatgattgt
     7621 tggaatccag gaggtttgag cgcgagatat tacggattgg ggtcggctca gaaaacggaa
     7681 aaaatcgtac gctaagccga agttcagcac tcacgcattc tgtcgagcac gtaccagttc
     7741 cgtgaaactg acaacatcca tcaaccatgc ctgctgggct ggcgtatagg tctggtgcag
     7801 actgcgcggc agaacgagtt gcagccgctt tgctgccatt tcgtcggtct gatgcgtgga
     7861 gattgctgtt tccagcgtca gcagatgctt gtcatcaatg cggtctgctt cggcgagaac
     7921 ctgccgccag cggtccttgc aggtcgattt cacaccaagc atggtcagct tcagcgagtc
     7981 gaacgccggg ttatggtatt cggggttgta agccggaacc gccgaaattt ccgtcaactc
     8041 ctctaacccc ctgccacaag ggcatctagg tcaatcatct tgcggtcact ctgaaagatt
     8101 taattcttta ccaaccccaa cccctgcaac gaagtcggga tcgtgagaaa ccaatagcag
     8161 agtgcctggg tagtccgcca ggacctgagt gagcaactga cgcgaggggt gatccagatg
     8221 gttgtccggt tcatcgagca gcagcaggtc gggcagttgc gccagtacca tcagcagacc
     8281 cagttttaac ctctcccccc cactcagacc atgacaaggc gtcagagcac gatcgccgcg
     8341 cagggctatg cccgccagcc gtgttctcgc atcactctcg gccagtgtcg gagccactgt
     8401 caacaggttg gcaagcgggc tgagatcccg cgcaatcagg ctgagcccct gatcgaggca
     8461 agccatcttg ccattgatgc gaaagctgcc cgtctcgggc tccagcaccc cacgcaacat
     8521 ctgcagcaga ctcgatttgc cgcagccatt gcgaccgccg agcaaacagc gctcaccccg
     8581 ttgcagcacc agatcaaccc gctggccatg ccagcgcacg tgagacgcag ccgtaatcac
     8641 cagcggctgg gcagccccct tgcgctcgat ctcctccggc cactgcatga tgagcggctg
     8701 caccgccgcc agcgcggctg ctgcctcggt gcgggcctcc acttgcgcac tgtggccccg
     8761 ctcgtggcgg tccagcactc tgccaaggga tttgcctgcc tgttccgcct tgaaatcaag
     8821 caggattttg gcttgggagc cgcgctcacg ccgcccctga ctggctcgtc tggccgcctg
     8881 ttcacgcact ttttgcaagc tgcgcgccgc cgccttttcg cttcgcaccg cttgcgccaa
     8941 actggtgctg cgggcagcct gtacctcggc ctgctgtgcc agaaactcgt caaacgagag
     9001 gcgggcactc tgcaacccct gctcgctcag ctccagaata tgactggttt gattgagcag
     9061 gcgccggtcg tggctcacca gcaggtaacc tccgcgatgt ctggccagtt gctggcttag
     9121 ccagtcgatg ccagaggcat cgaggtggtt gtctggctca tcaagcaaca gaaagtgagc
     9181 aggcctcgca aacagcgcca gcaggcgcaa acgggtgagc tggccaccgc tcagggtgct
     9241 gaccgggaca tggggatcga gtctgaggcc tgcttctgcc agcaggtcgg caagatcgct
     9301 gcgcaggttc cagcgatcag ccagcaactc gaaatcgtcc tgggcgcagc tcccctgctc
     9361 gatgcgctca agggcccgta gcggctcgcc ccagccgata aggtcggcca cactggctgc
     9421 gcagtcatga tcttgagccg gtgcaaactg ggacaaccag ctgatggggg tcaaccactc
     9481 gatcttgcca gaatccgcgc ggcgctggcc cgccagcagt tccagcagca ggctcttgcc
     9541 gatgccgtta cggccgatca gggtgcagcc cgcagcgggc aaagtgaagg taaggggcac
     9601 agtacaggcg acgaagggca gctgcaaagt gttgatatga catgcgatat gggtcatctg
     9661 aactcctgta ggccaatgcg gaagccgcag gccaagagct aaaccaaatg acagggacac
     9721 gtgtccacta gtgggaaaac agcagaggaa tcagcatcaa cctgtcacgg tgagacgcac
     9781 ccgctctccc tggcgggaga agaatcaaaa gtggggtgcg gacaggttaa atgcgcatcg
     9841 gcgaaatact ctttactgat gaaagtaaaa tcatacttca gatagtaaaa aatgccaaat
     9901 gttccggtat cactcacttg catgttgtat caataagtca agtgtcagtc tgggctatac
     9961 cctatcctga tgtcagatcg ttcggttaaa aagtgtcaga ataaagcagt taaattaatt
    10021 tattgacaca cataaggcaa ggtcatagat ttcttcctga cacttttaag ctctggaggt
    10081 ctcttgcagg gacaacgcat cggttacatc cgtgtcagta gcttcgatca aaacccagaa
    10141 cggcaactgg aacatatcca ggtggacaaa gttttcatcg acaaagcctc tgggaaagac
    10201 actcagcgcc cgcagcttga cgtcttgctc tcctttgtac gtgaaggtga cacggtcgtg
    10261 gtacacagca tggatcgcct tgctcgcaac ttggatgatc tgcgccgact tgtacaggga
    10321 ctaacccagc gccgcgtctg cattgaattc gtcaaggagc atctgacttt caccggcgaa
    10381 gattcaccga tggcaaacct tatgttgtct gtcatggggg cattcgccga gttcgagcga
    10441 gcgttgatcc gggagcgtca acgtgaaggt attgccttgg caaaacagcg tggcgcttat
    10501 cggggtcgaa agaaatcgct gtctgaccca gagatagcgg aagtacggcg gcgagtcatt
    10561 gctggcgaaa agaaagcgac gcttgcccgt gaattcggga tcagtcgaga aacgctgtac
    10621 caatacttgc gggtgccgga gtaactcatg gcgattgcta aaagcgagcg actaacagtt
    10681 ctgtcggacg ccgagcagga ggcgctgtat ggcttgccgg actttgacga tgcgcagcgc
    10741 ctggaatacc tggccttgac cgaaaatgaa ctggcgctcg ccagcagccg gccaagcatc
    10801 catgctcagg tctattgcat tctgcagatc gggtatttca aagccaagaa cgatttcttc
    10861 cgtttctact ggcgtgacgt cgaggatgat tgcgctttcg tgctcagccg atattttcaa
    10921 ggtgaggcat tcgagaccaa gcagatctcc aaacacgagc actacagcca acgcggtaaa
    10981 attgccgcac tattcggtta ccagccgtgg gcctccagct acctgccatc gcttgagcat
    11041 caagcctcgc tgatcgtgcg gcgtgatgtg acgcccaact ttgttgctgc tgagctgatc
    11101 gtctggctca acgaacaaaa gatcattcgt ccaggctata ccaccctgca agcgctggtc
    11161 agcgaagtcc tgtccgctga acgtcgacga ctgggagccc tgcttgcaca ggtgttggat
    11221 gaaccagcca agacttccct gagtcagctc ctggtgcgcg acgatacgct gtcgcaactg
    11281 gctgctctca aacaagacgc caaggatttc gggtggcgtc agatggcccg agagcgtgac
    11341 aagcgtaccc tactggcgcc attacatggg attgccaaag ggctgctgcc caaacttggg
    11401 atctctcaac aaaacctgct gtattacgcg agtctggcta acttttatac cgtccatgat
    11461 ttgcgcaact tgaagccaga gcagacccag ctctatctgt tgtgctatgc ttgggtacgc
    11521 taccggcagc tcagcgataa cctggtcgat gcgatggcgt tccacatgca tcaacttgat
    11581 cacgaaaacc gcgcgggtgc cagggagtct ttcgccgaag agcaggtcaa acgtcatcag
    11641 gaaacgcccc aggttggtcg cttgctgtcc ctgtacgtgg acgacagtgt cgcggatccg
    11701 acgccattcg gcgaggtgcg ccagcgcgcc tacaagatca tggccaaaga cgtgctgcaa
    11761 aacacggcgc agcgcatgag tgtcaagcca ttgaataaac tgacattgca ctggcaggcg
    11821 gtggatggcc aggccaaacg tatcagccgt catctccgac cgttgtttgt cgcgctggag
    11881 ttcgccgcta ccgatccgga cagccagtgg ctggcggcac taacctgggt taaaagcgtg
    11941 ttcgccaagc agctgcgtct gtcacaacgc ccactcgctg aatgcccggc agccacccta
    12001 cctaaacgct tgcggcccta cctgctgagc tttgatgcag atggagagcc gacagggtta
    12061 catgccgacc gctacgagtt ctggttgtac cgccaagtca caaagcgttt ccaatctggc
    12121 gagctttatc tcgatgacag tttgcaacat cggcatttct ccgatgagtt ggtgtctatg
    12181 gaagagcacg ctcaggagct ggcacagatg gacatcccat tcctgcgcca accggttgaa
    12241 acacagcttg atgccttggc ctccgagctg cacaagcaat ggcgggcatt caaccgtgag
    12301 ctgaaacaag gcaagctgac acacctcgaa tacgacaaag acacgcaaaa actgacttgg
    12361 cgcaagccca agggggaaaa ccaaaaggcg cgggagcaaa ccttttacga tcaattgcca
    12421 tactgtgatg tcgccgacgt gttccgcttc gtcaacaacc aatgccagtt cctatcggca
    12481 ctgactccgc tgcaaccacg ttacgccaag aaggttgcgg ataccgatag cctgattgcg
    12541 gtcatcatcg cccaggccat gaaccacggc aacctggtca tggcgcgtac cagtgatatt
    12601 ccataccacg tcctggagag cgcctatcag cagtacctgc ggcaagcatc gctacacgag
    12661 gccaacgacg gcatcagcaa tgccattgcc gcgctgccta tcttcccgta ttactcgttc
    12721 gacctcgatg tgctgtatgg cgctgtcgat gggcagaaat tcagcgtcga gcggccaacc
    12781 gtgaaagcgc gctactcgcg caagtatttc gggcgaggca agggcgtggt ggcctatacg
    12841 ctcttgtgca accacatccc gttgaatgga tacctgattg gtgcccatga ttatgaggcc
    12901 caccatgtgt tcgacatctg gtatcgcaat acctctgaca ttgtgccaga ggtcattact
    12961 ggcgatatgc acagcatcaa caaggccaat ttcgctatcc tgcactggtt cgggcggcgt
    13021 ttcgagccac ggtataccga cctcaacgcc cagttgcagg cgttgtattg tgtgggcgat
    13081 ccagcacggt atgagaagtg cctgatccag ccagttggcc aaatagatcg gcagttgatt
    13141 gtcagcgaaa aggcgaacat tgaccggatc atcgccaccc tgggtttgaa ggagatgacg
    13201 cagggaacgt tgatccgcaa actgtgcacc tacaccgcgc caaacccgac gcggcgggcc
    13261 atcttcgagt tcgataagct cgtgcgcagc atctacacgc tacgctacct gcgcgacccc
    13321 caattggaac gtaacgtcca tcgctcgcaa aaccggattg agtcatacca tcagctacgt
    13381 tcaagcattg cgcaggtggg aggcaagaaa gaattgacgg ggcggaccga cattgagatc
    13441 gagatctgca accagtgcgc caggctgatc gccaacgcag tcatctacta caactcggcc
    13501 atcctgtcgc ggttgctaac gaagtgcgaa gcaacaggta acgccaaggc gctcgcggcg
    13561 ctcacccaga tatcgccggc agcctggcga cacattttgc tgaacggaca ctataccttt
    13621 cagagtgacg gcaaggtgat tgatctggat gcccttgtgg caggggtgga actggcgtga
    13681 cggaaatttc ggcggttccg gcttacaacc cctgttagat gccagcccga tcaatgtgcg
    13741 ctgaccttgg atagcagatt tagaacggcg acgccactaa cgataagtcc catgccaacg
    13801 aacgcccaca agtctagttt ctggccatgg aagatccaag cgatagctgc cacaagtacg
    13861 atgccgaggc cagcccaaac agcataagca atgccgaccg ggatggactt gagtgcgaga
    13921 gagaggaaat agaacgcaag cccgtagcca gccacaacta caacagaagg aactaacttg
    13981 gtgaatccat ggctggactt cagtgcggaa gttgcgacga cctcaccaaa tattgcaata
    14041 gccagaaaga gccagttctt cacgtgcaat ctcctctacg gtatgaagga taaatagtgg
    14101 tggctatgag ttgccaaaaa cagtcttgcg gctgtcgatt ttctgtgagc atacgcaacg
    14161 ccaaatctgg catctaacgc ttgagttaag ccgcgccgcg aagcggcgtc ggcttgaacg
    14221 aattgttaga cattatttgc cgactacctt ggtgatctcg cctttcacgt agtgaacaaa
    14281 ttcttccaac tgatctgcgc gggaggccaa gcgatcttct tcttgtccaa gataagcctg
    14341 tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc
    14401 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt
    14461 aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt
    14521 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc
    14581 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat
    14641 gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa
    14701 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt
    14761 gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc
    14821 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc
    14881 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
    14941 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac
    15001 ttcggcgatc accgcttccc tcatgatgtt taacgcctga gctcagccga ccgaaaccgc
    15061 gtagcggttt tgggtcggct gcagcgattt gttgggcgat agcttgccac attctctcaa
    15121 cgattgggat ttgatgtact ttccgcactt tgtagcgcga ccacatcatg ctccccctgg
    15181 ccgcgagagc ccttcactcg ggaaacacaa gacagaccga gcacgactgt tgcaagggtc
    15241 aaacagtaca cgacaaccgg ccaagccgtg ttgcgcggca acagcgaaat gatcaacgtt
    15301 ccgatgcttc ctagcagtac accgcccaag cagaagtaga ctgccgtgac cgttccagca
    15361 acatggtcga atcctcgaag agcgccattg ggcgccacag ataccgctgt ggcgacacca
    15421 atacccacta gccacattgg agcaataaag cctaacacgg actgcaaagc ccatatttcg
    15481 gtgatggcaa gcaatactgc tccagctatc aggcatccca ttcccattcg caagacactt
    15541 gggctgcccc acttgggtat cacacgcccc ataaaacgag ccgtaaacac catggcaatt
    15601 gccactgtgg cgaacagcag gctgaagcca agctgagaca caccttgcct gcccatcatt
    15661 agtccgggcg caatggagaa aaagacgaag aagctaccca ttccagcggc gtaacacaac
    15721 gtgtacaacc agaagttcag gcacttaacg gggagtagca gctgcgacca ttgcaagccc
    15781 gcaactcgtt gcacccgggt ttcaggccag aatcgccacg ctgctgcaga tgcagcgatc
    15841 atgcccaaac ctagaaacgc aaagatagcc cgccacccaa gccacatgtc gacgagcgct
    15901 ccgagcaatg ggcctaccgc cgggaccatg gccagcatgg atccgagtat gccgtaaatg
    15961 acattacttt cctcgcgacc tgcgtaaatg tcacgtactg ttgcaaatgt ggaaacaagg
    16021 cacgccgagg caccacaagc ctgaagaatc cgaagcccca gaaagacttc agccgatgac
    16081 gtaagagcga ggcccattga cgccacaacg taggcgaggc cacctcccag tagaacgggg
    16141 cggcgcccca gtcggtccga tagcggtcca aacaagagct gaccggcacc aatcatgacc
    16201 aagtacgttg tcagcgtaag ctgaattgtg ctcgctgtcg taccaagcgc gtttggcata
    16261 aacggcactg ctggcaagta catgtccatg ccgagtgatg ccaataaatc gaacggtgat
    16321 aacaacaaca ccgtggcggc aagggagtac cgccaactaa agttttttga gctcacgaaa
    16381 ccatctcctt gaacaaagga tttggcggcg ctcccgtaga gcaaaaggat tcatgagaac
    16441 gccgcaacaa ccgaaaaatg aaggttgctg cggcttactt gtctgcgttc ttggaagtgc
    16501 tcatctgctg actatctcat gattgaattt gaatcgtaac aactttcaag caactctgca
    16561 aggagcctta ttgtgcgccc aacgccggag ttaagccgcc gcgcgtagcg cggtcggctt
    16621 gaacgaattg ttagacatca tttaccaact gacttgatga tctcgccttt cacaaagcga
    16681 ataaattctt ccaagtgatc tgcgcgtgag gccaagtgat cttctttttg tcccagataa
    16741 gcttgcttag cttcaagtaa gacgggctga tactgggcag gtaggcgttt tattgcccag
    16801 tcggcagcga catccttcgg cgcgattttg ccggttattg cgctgtacca aatgcgggac
    16861 aacgtaagca ctacatttcg ctcatcgccg gcccagtcgg gctgcgagtt ccatagcttc
    16921 aaggtttccc tcagcgcctc gaatagatcc tgttcaggaa ccgggtcaaa gaattcctcc
    16981 gctgccggac ctaccaaggc aacgctatgt tctcttgctt ttgtaagcag gatagctaga
    17041 tcaatgtcga tcatggctgg ctcgaagata cccgcaagaa tgtcattgcg ctgccattct
    17101 ccaaattgca gctcgcgctt agccggataa cgccacggga tgatgtcgtc atgcacgaca
    17161 agggtgactt ctatagcgcg gagcgtctcg ctctcgccag ggaaagccga agcctccata
    17221 agatcattga gcaatgctcg ccgcgtcgtt tcatcaagct ttacggccac agtaaccaac
    17281 aaatcaatat cgctgtatgg cttcaggccg ccatccactg cggagccgta caaatgcacg
    17341 gccagcaacg ttgattccag atggcgctca atgacgctta gcacctctga tagttggttc
    17401 gaaatttcga tggtcaccgc ttccctcatg atgtctaacg ggcgaggtaa gccgaccgca
    17461 gaatgcgggt cggcttgacc gaaatgttag aaccagaagc caaaacggat aacttgaatt
    17521 tggcgacggg cgctaaccgt gaaaaaacgc tgcgccaccg aggcggcaca gcactgcaaa
    17581 aacgatagct gcttgcgctt gctacgcaag ggctagaggc caaaaagact gaaaacctgc
    17641 gcagcccatg caggcgaagc ccggaaaaaa ggcaaaacag gcactgaata acgcctgaaa
    17701 agctaaatgc cgtttgaata aacatgagct aaataaagct gggtttcagt ggtgctaacg
    17761 ttggacgtaa cgagagccgg agcgcagcgg agggaaccaa aatgcgcagc attttggcgt
    17821 cccgttgacg gaatggttag ccgtttcgac gcgcataaac ggagtgggtg tacggaatta
    17881 cagcttgaat ggtttcggtt gagacaagct cgaattctgt ttcgttgagc attgggaaga
    17941 aggcgtcacc ctcgaaggtt tgatgtacct cagatagaaa cacgccgtgg gcgtgaggta
    18001 gtgccagagt gtatatctca gctccgcccg cgacgtagag ttcattgccg agttcggatg
    18061 ccaaagcgat agcgtgcgac agcgttgaaa caactacgca gccagtggcg cggtagttag
    18121 cttggcgtga gattaccaat gtgtgacggt tcggtagagg cttgccgata gactcaaagg
    18181 tctttcgccc catgacaacg acttttccct cagtgagtct gcgaaaaatc ttctgctcac
    18241 ccggaatttt ccaggggata ttaggaccat tgccaataac ccgattggct cccatcgcag
    18301 caacgagata aatgcgtact gattccgagt tcatatggct aactttgttt tagggcgact
    18361 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
    18421 cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat
    18481 gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg
    18541 tgagcgcata cgctacttgc attacagctt accaaccgaa caggcttatg tccactgggt
    18601 tcgtgccttc atccgtttcc acggtgtgcg tcacccggca accttgggca gcagcgaagt
    18661 cgaggcattt ctgtcctggc tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca
    18721 ggcattggcg gccttgctgt tcttctacgg caaggtgctg tgcacggatc tgccctggct
    18781 tcaggagatc ggaagacctc ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga
    18841 agtggttcgc atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc agcttctgta
    18901 tggaacgggc atgcggatca gtgagggttt gcaactgcgg gtcaaggatc tggatttcga
    18961 tcacggcacg atcatcgtgc gggagggcaa gggctccaag gatcgggcct tgatgttacc
    19021 cgagagcttg gcacccagcc tgcgcgagca gctgtcgcgt gcacgggcat ggtggctgaa
    19081 ggaccaggcc gagggccgca gcggcgttgc gcttcccgac gcccttgagc ggaagtatcc
    19141 gcgcgccggg cattcctggc cgtggttctg ggtttttgcg cagcacacgc attcgaccga
    19201 tccacggagc ggtgtcgtgc gtcgccatca catgtatgac cagacctttc agcgcgcctt
    19261 caaacgtgcc gtagaacaag caggcatcac gaagcccgcc acaccgcaca ccctccgcca
    19321 ctcgttcgcg acggccttgc tccgcagcgg ttacgacatt cgaaccgtgc aggatctgct
    19381 cggccattcc gacgtctcta cgacgatgat ttacacgcat gtgctgaaag ttggcggtgc
    19441 cggagtgcgc tcaccgcttg atgcgctgcc gcccctcact agtgagaggt agggcagcgc
    19501 aagtcaatcc tggcggattc actacccctg cgcgaaggcc atcggtgccg catcgaacgg
    19561 ccggttgcgg aaagtcctcc ctgcgtccgc tgatggccgg cagcagcccg tcgttgcctg
    19621 atggatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc
    19681 ttctgaaaac gacaatggag gtggtagccg agggtgtgga aacacccgac tgccttgcgt
    19741 ggttgcggca ggcgggttgc gacacggtgc agggtttcct gttcgccagg ccgatgccgg
    19801 cggcggcctt cgtcggcttc gtcaaccaat ggaggaacac caccatgaac gccaatgaac
    19861 cgagcaccag ttgctgcgtg tgctgcaagg aaatcccgct cgatgccgcc ttcacgccgg
    19921 aaggggccga gtacgtggag catttctgcg ggctggagtg ctatcagcgc ttccaggcgc
    19981 gggccagcac tgcgaccgaa accagcgtca aaccggacgc ttgtgattcg ccgccgtcag
    20041 gttgaggcat accctaactt gatgtcaggc agggccgcgc cgcttcgtca gaatagagtc
    20101 tgctttccca ttttttgaca catgcccgcg aaggttatag atttcagcct gacagaaatg
    20161 ggctttgagg cacaacggaa cagaaagtgc acttaagccg ccttcaacca aggagacatc
    20221 gtgcaggggc accgcatcgg ctacgtccgg gtcagcagct ttgaccagaa cccggaacgc
    20281 cagctggaac aaacccaggt gagcaaggtg ttcaccgaca aggcatcggg caaggacacc
    20341 cagcgccccc agctcgaagc gctgctgagc ttcgtccgcg aaggcgatac agtggtggtg
    20401 cacagcatgg atcggctggc ccgcaacctc gatgacctgc gtcgcttggt acagaagctg
    20461 actcagcgcg gcgtgcgcat cgagttcctg aaggagggcc tggtgttcac tggcgaggac
    20521 tcgccgatgg ccaacctgat gctgtcggtg atgggggcct tcgctgagtt cgagcgcgcc
    20581 ctgatccgcg agcggcagcg tgagggcatc gccttggcca agcagcgtgg cgcgtaccgg
    20641 ggccgcaaga aagccctgtc cgatgagcag gctgctaccc tgcggcagcg agcgacggcc
    20701 ggcgagccca aggcgcagct tgcccgcgag ttcaacatca gccgggaaac cctctaccag
    20761 tacctccgca cggacgactg acacatgccg cgtcgcttga tcctctcggc cacggagcgg
    20821 gacaccctgc ttgcgctgcc ggaaagccag gatgacctga tccgctacta caccttcaac
    20881 gactccgacc tgtcgctgat ccgccagcga cgcggcgacg ccaaccgcct cggcttcgcc
    20941 gtgcagctct gcctgctgcg ctaccccggt tacgcgctgg gaaccgacag cgagctgccc
    21001 gagccggtca tcctgtgggt ggcgaagcaa gtccaggccg agccggcgag ctgggcaaag
    21061 tacggcgagc gcgacgtgac ccgtcgcgag catgcccagg aactgcgcac ctacctgcaa
    21121 ctggccccgt tcggcctgtc cgacttccgc gccctggtgc gcgagctaac cgagctggcc
    21181 cagcagaccg acaaaggctt gctgctggcc ggtcaggccc tggagagcct acggcagaaa
    21241 cgacgcatcc tgccggcgct gagcgtgatt gaccgggcct gctcggaagc cattgcgcga
    21301 gccaatcggc gggtctaccg cgccctggtc gaaccactca cggactcgca tcgggccaag
    21361 ctggacgagc tgttgaagct caaggccggc agcagcatca cctggttgac ctggctgcgc
    21421 caggcaccgc tgaaacccaa ctctcggcac atgctggaac acatcgagcg gctgaagaca
    21481 tttcagttgg tggacttgcc cgaaggcctg ggccggcaca tccaccagaa ccgcctgctc
    21541 aagctggccc gcgagggtgg gcagatgacg cccaaagacc tcggtaagtt cgagccgcag
    21601 cgccgctacg cgaccctggc cgccgtggtg ctggagagca ccgcgaccgt gatcgatgag
    21661 ctggtcgatc tgcatgaccg catcctggtc aagctgttca gcggcgcgaa gcacaagcat
    21721 cagcagcagt tccagaagca gggcaaggcg atcaacgaca aggtgcgcct gtactccagg
    21781 atcggccagg cgctgctgga agcgaaggaa agcggcagcg acccctatgc cgccatcgag
    21841 gcggtgattc cctgggacga gttcaccgag agcgtcagcg aggccgagct gctggcccgg
    21901 ccggaaggct tcgaccacct gcacctggtc ggcgagaact tcgccaccct gcgccgttac
    21961 acgccggcct tgctggaggt gctggaactg cgcgccgcgc cggccgcgca aggcgtgctg
    22021 gcagccgtgc agaccctgcg tgagatgaac gccgacaacc tgcgcaaggt gccggccgat
    22081 gcacccacgg ccttcatcaa gccgcgctgg aagccgctgg tgatcacccc ggaaggcctc
    22141 gaccggaaat tctacgaaat ctgcgccctg tccgagctga agaacgccct gcgctccggc
    22201 gacatctggg tcaagggctc gcggcagttc cgcgacttcg acgactacct gctgccggcc
    22261 gagaagttcg ccgcactcaa gcgcgagcag gccctgcccc tggcgatcaa cccgaacagc
    22321 gaccagtacc tggaagagcg tttgcagctg ctggacgagc agttggcc
//
