LOCUS       NZ_OP242287             4145 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA153 plasmid pLAO67, complete sequence.
ACCESSION   NZ_OP242287
VERSION     NZ_OP242287.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363353
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Sequencing Technology             :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028600715.1-RS_2024_03_23
            Annotation Date                   :: 03/23/2024 03:26:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6
            CDSs (total)                      :: 6
            Genes (coding)                    :: 2
            CDSs (with protein)               :: 2
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242287.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA153"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO67"
     gene            join(3044..4145,1..41)
                     /gene="wzz(fepE)"
                     /locus_tag="PUP25_RS00005"
     CDS             join(3044..4145,1..41)
                     /gene="wzz(fepE)"
                     /locus_tag="PUP25_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435173.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="WP_021544858.1"
                     /translation="MSAMDFKKNTDLDFPRYATPAVSTKEIDLLGLLDVLLAAKKRIVT
                     IVLAFAVVGLAIAFLIPQKWTSKAVITPAEQTQWNTLRQMMVTLQVLDVDAKVSRGDVF
                     NLFIKKFQSQSLLEEYMKTSPYVMSQLEGTEVDPLELHRAVVNIAEKMKATDNTQVKDA
                     DKAPYTSWTLSFTAPTAGDAQTVLEGYINYISRIVEQETMENIRNQITLKTKVVQQQLE
                     LDRVRLTNIHNTNLQRLNYSLEVANAAGIKKPVYSNGQAVKDDPDYSVALGADGIAQKL
                     QIEKNLKDVAELNADFQNREYYLTQLQKLSVEDVKLEPFRYQLSPSLPVKKDGPGKALI
                     VLLAGILGGLFACGSVLLREAMLSRNPLPEPITAVTPATE"
                     /name="wzz(fepE)"
     gene            complement(79..>321)
                     /locus_tag="PUP25_RS00010"
                     /old_locus_tag="KLPFCKAP_00001"
                     /pseudo=""
     CDS             complement(79..>321)
                     /locus_tag="PUP25_RS00010"
                     /old_locus_tag="KLPFCKAP_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003862821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /name="cupin domain-containing protein"
     gene            <318..500
                     /locus_tag="PUP25_RS00015"
                     /pseudo=""
     CDS             <318..500
                     /locus_tag="PUP25_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007798797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /name="NINE protein"
     gene            1686..1799
                     /locus_tag="PUP25_RS00035"
     CDS             1686..1799
                     /locus_tag="PUP25_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071778980.1"
                     /translation="MHEPPVQSDRCVFSGNYRLESKPERHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            2107..>2238
                     /locus_tag="PUP25_RS00020"
                     /old_locus_tag="KLPFCKAP_00002"
                     /pseudo=""
     CDS             2107..>2238
                     /locus_tag="PUP25_RS00020"
                     /old_locus_tag="KLPFCKAP_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /name="zinc-ribbon domain-containing protein"
     gene            complement(2387..2849)
                     /locus_tag="PUP25_RS00030"
                     /old_locus_tag="KLPFCKAP_00003"
                     /pseudo=""
     CDS             complement(2387..2849)
                     /locus_tag="PUP25_RS00030"
                     /old_locus_tag="KLPFCKAP_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     Typing          1415..1803
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.153"
                     /coverage="91.0"
                     /evalue="1.04e-173"
ORIGIN
        1 tgccagagcc gataacggcc gtaacgccag caacagagta acgacgatat taaaaaggcg
       61 ctgattcagc gccttttttt atttcaggaa atcgtcgcgc accggcgtaa aggtatccag
      121 aagggtgccg ggcttcaggc aaatgcagcc gtgcatcacg tgcggcgctt tatagagcgt
      181 atcgcccgcc gtcacgatct gcttttcttc acctatcgta aactcgaact ccccggagag
      241 cacataggta agctgttcat gcgggtgatt gtgcatcggg ccaatcgcac cctgctcaaa
      301 attgacctcc accgccatca tctttcttag cattcttcct tggtggattt ggcgtacata
      361 aattttactt aggtaaaata ggacagggat tcctgtatct aattttttgc tggacattca
      421 tacccgcgat catcgcgttt attgagttta ttatttatct atgcgattcc gatgaaaaat
      481 tcgcaagaaa atatggctaa ctcatagcaa aaaagccctg cggggctttt tttgtaccag
      541 tctgtgaggc agatttacca taacatccgt tgcgtgcacc gcctgagagg ctctcagggc
      601 gtttctgggt cttcctgcct gtttccgaca cttccctgtg cctatatcgt gaaaaaacgc
      661 gcctgatgat tctggcgcgt ctgcatggct atgcacacac atgcatggcc gggattttag
      721 cggctaaaat ccggctggac gcccaaaagc accactgaac ctcacgaata acgcctcacg
      781 ggtgtgcagt ttactgtgcg gccgtgaggc gttggccagg ccgtcaggca tggctggatt
      841 tattgctaag ggggtcggat ttaacgtgga tgaatcagca cgcagtgatg aacgggattc
      901 ggaaggggca cccttgcggc gcggcgatgt atacatcgct aacgtgcctt ggtcatttat
      961 tgcaagaagg gagaggtagc attttgatag caaaaaagta gcaaaaggat agcaaccgaa
     1021 agaccagaag actctggaga tagcaaaaaa gtagcaaaat ggtagcaaaa agatagcaaa
     1081 catgcgggag ctaaccagga gtctttagcg ggccggcggt actccaaaaa cacatcttgt
     1141 cccgctgttt tgtgaataag ccagcatagc tgtgggcaca aaacatttcc tgtccgctgc
     1201 tttaaccttt gtaatccagg aacgtcaggt gacgtcactt tgcggcgtta gccctatcct
     1261 gcatcgtatt aaaaaatgca gcactggcgc gtacggtgcc gaagataagt ccggggatat
     1321 tccgcttcct cgctcactga ctcgctacgc tcggtcgttc ggctgcggcg agcggaggga
     1381 acggagacat taaggcacag gcaatgagag aggatcgaga ggcaaaagaa aaggccgcgg
     1441 cgttgccggt tttccatagg ctccgccccc ctgacaagca tcacagaatc tgacgctcaa
     1501 atcagtggtg gcgaaacccg acaggactta aagataccag gcgtttcccc ctggtggctc
     1561 cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc
     1621 gcgtttatct cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg
     1681 actgtatgca cgaacccccc gttcagtccg accgctgcgt cttctccggt aactatcgac
     1741 ttgagtccaa gccggaaaga cacgacaaag cgccactggc agcagccact ggtaactgat
     1801 ttagaggagt tagtcttgaa gttatgcgcc tgttaaggct aaactgaaag aacagatttg
     1861 gttactgcgc tcctcataag ccagttacca cggttcaaag agttgcagct ctgagaacct
     1921 tcgaaaaacc gccttgctgg gcggtttttt cgtttactga gcaagagatt acgacgctac
     1981 agaaaggatc tcaagaagat ccttcaccat aatggaaatc tatcccaccc gagcatatag
     2041 gatgcatatc gttataaaac atcggatatt atgcatccca actaaaaata agctcgagga
     2101 taaaaaatga gttcaatggt ttactgccga ggatgcggca aggaaataca cgaaacagca
     2161 aagtcatgcc cacactgcgg agccacaaac gcctcttcag gttcaggtga aaaaagtaga
     2221 atcgcagccg cgctattagg gaaggtgcga ataagcgggg aaattcttct cggttgactc
     2281 agtcatttca tttcttcatg tttgagccga tttttttctc ccgtaaatgc cttgaatcag
     2341 cctatttaga ccgtttcttc gccatttaag gcgttattcc cagtttttag tgagatctct
     2401 cccactgacg tatcatttgg tccgcccgaa acaggttggc cagcgtgaat aacatcgcca
     2461 gttggttatc gtttttcagc aaccccttgt atctggcttt cacgaagccg aactgtcgct
     2521 tgatgatgcg aaatgggtgc tccaccttgg cccggatgct ggctttcatg taaccgggca
     2581 aacagacgca tggaggcgat ttcgtacaga gcatcttcga tcgcgccatc gctcaggttg
     2641 taccaatgct gcatgcagtg aatgcgtagc atggtttcca gcggataagg tcgccggcca
     2701 ttaccagcct tggggtaaaa cggctcgatg acttccacca tgttttgcca tggcagaatc
     2761 tgctccatgc gggacaagaa aatctctttt ctggtctgac ggcgcttact gctgaattca
     2821 ctgtcggcga aggtaagttg atgactcatg atgaaccctg ttccatggct ccagatgaca
     2881 aacatgatct catatcaggg acttgttcgc accttcccta aatctgatag tcggtctaca
     2941 gatagatcaa tctggcgttc agattattct tactttcttg tatcatccga ccactgcaac
     3001 caatcaggca gaaatttact gcgctccaaa aagaagaacg gttatgtcag cgatggattt
     3061 caagaaaaac acggacctcg attttccccg ttatgccact ccggcggtaa gcaccaaaga
     3121 gatagatctc ctggggttgt tagacgtact tctggcagcc aaaaaacgta ttgtgacgat
     3181 cgtcctggcg ttcgccgtag tagggttggc cattgcgttt ttaatcccgc aaaaatggac
     3241 cagtaaagcc gtgatcacgc cggccgagca gacgcagtgg aacacgctgc gccagatgat
     3301 ggtgacgctg caggtgctgg acgtcgacgc gaaggtgagc cgtggagacg tgtttaacct
     3361 gttcatcaaa aagtttcagt cgcagtcgct gctcgaagag tacatgaaaa cctccccgta
     3421 cgtgatgtct cagctggaag gcaccgaggt agacccgctg gagctgcatc gcgcggtggt
     3481 gaatattgct gagaaaatga aagcgacaga taacacgcag gttaaagacg cagacaaagc
     3541 gccgtatacc tcatggacgc tgagctttac cgccccgacg gcgggagacg cgcagacggt
     3601 tctggaaggt tacatcaact atatctcccg cattgttgag caggagacga tggaaaatat
     3661 ccgtaaccag atcacgctga aaacgaaagt cgttcagcag cagctggagc tggatcgcgt
     3721 gcgtctgacc aatatccaca acaccaacct gcagcgcctc aactattcgc tggaagtggc
     3781 gaacgcggca ggcattaaga agccggttta cagcaacgga caggcggtaa aagacgatcc
     3841 tgactactcc gtggcgctgg gtgctgacgg tattgcgcaa aagctgcaga ttgaaaagaa
     3901 tctgaaggac gtggccgagc tgaatgcgga tttccagaac cgtgaatact atctgactca
     3961 gttgcaaaaa ctgtccgtgg aagacgtgaa gcttgaaccc ttcaggtatc agctttcacc
     4021 gtcactgccg gtgaaaaaag acggtccggg caaagccctg attgtgctgc tggcagggat
     4081 tctgggcggc ctgtttgcct gcggcagcgt gctgctgcgt gaggcaatgc tctcgcgtaa
     4141 tccgt
//
