LOCUS       NZ_OP242284             4073 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA133 plasmid pLAO64, complete sequence.
ACCESSION   NZ_OP242284
VERSION     NZ_OP242284.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363352
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600685.1-RS_2024_03_23
            Annotation Date              :: 03/23/2024 03:19:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242284.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA133"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO64"
     gene            complement(86..2233)
                     /gene="mobQ"
                     /locus_tag="PUP26_RS00005"
                     /old_locus_tag="OCKBKEMH_00001"
     CDS             complement(86..2233)
                     /gene="mobQ"
                     /locus_tag="PUP26_RS00005"
                     /old_locus_tag="OCKBKEMH_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_032495499.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGKSGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            2412..2666
                     /gene="mobC"
                     /locus_tag="PUP26_RS00010"
                     /old_locus_tag="OCKBKEMH_00002"
     CDS             2412..2666
                     /gene="mobC"
                     /locus_tag="PUP26_RS00010"
                     /old_locus_tag="OCKBKEMH_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            3200..3901
                     /locus_tag="PUP26_RS00015"
                     /old_locus_tag="OCKBKEMH_00003"
     CDS             3200..3901
                     /locus_tag="PUP26_RS00015"
                     /old_locus_tag="OCKBKEMH_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(1876..2234)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2235..2411
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          3201..3901
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(90..2320)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.989"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttcttgcatt catccctgtc gatgaacatc gaagaacatc gaagaacata acacgatgtt
       61 aaacaaaaca aaatattgtt cacattcact gtgaccaacc gcgatcatgt ctcctctcgg
      121 tggtgtgtgt tcgtgcatct tcctcgcgct gccattcctc ccaggtacga ttaaaaccca
      181 gcgagcggaa cagatgatcc ggctgttttt ctggtgtgtt gacggcagca aaaagcggtg
      241 cctcacgggt tttcagatga aactgcatca gttctggtgc aggtgatatg tggctgtctg
      301 catcgtgatt tacggcaaca ccataccctg cataccgtaa ccccttatcg gtcagcgcac
      361 tgatgaccag atgcctgtta tcggctgagg gaacggcaaa cgcgggatac agcgtaccat
      421 taagccggat aacaccgccc agagagtcat ccacgcccat gaccagcaaa agctgaagct
      481 ggcgggtttc ccggtcgaca gaactcatga ccggcatcag cgtcggtggc agacgtccct
      541 gcataaaatc caccttcctt gccgctggtg atggcatggc tggctggcgg tcttctccaa
      601 gacagaaaga ctccggccat gtcttcggct ggcggagatt gcgggtatgt tgcggccatg
      661 tctggcaaac ctgcgtcagc agttgctcaa aggttgcaga ggaataaaca cgttgcagtg
      721 gcggacgttg gccgatttcg cttcggtcac agatataacc actcctgtca cgcattaaat
      781 caacactctc aaagattctg gtgcggtatt tctccagctc gttcccccag agcgtatatt
      841 ctccgttccg gttgtgaagc agaaggtaac aggtcatttc cccgttacca ggcatgggag
      901 cctcgccata ccccgtaacg actgctctca gcacccctga atccataacg gcattgtcat
      961 ttttcctgag ctgctccgcg cgactacgtt cagtatcttc ctgttccttc cggcgcaatt
     1021 cctcttcacg tttaagcaac tgggctctga gagctgcaag gccggatttc ccccactgct
     1081 gccactgttt ttcaaatgcc atctgactcg cgggaatgtc ctgtttctcc accttccggt
     1141 aatgctgtcg taatttttcc atttgctggc tcagagactg gatttttccc tgctcactac
     1201 ggtattccgg tgccgcccat tgcatcaggg gaatacggga aaaaccggac gaaaacggcg
     1261 atgatttttt caggctggca gcatggctca tcgcttcctt tcgttcggtg ttcagctctt
     1321 cccagcgtct gcgcgtcgcc tgcatatctg ccatcatcga catggcatta ctggtgagtt
     1381 tccatgccga gttactgtcc ttcggcaacg cttcgataat gtcacgtagt gtgtacgaag
     1441 ttgcgttgtc aggaagcccg atattcagaa ggtcagccac acggcgggca ggctctgtca
     1501 gccctccgtt cctgagttcg gtgcgggaaa tctgtatttc caggttaagc ctgttaatca
     1561 gacgattctg ttcgccgcgt tctgtttcga taccgcgtgt ttccatcgca caggcagact
     1621 ttccgaggtg aataccaggc gttttctcaa gcccctgtcg ctcgtatgaa cgatggtcta
     1681 tctcttcctg ataaccggcg ttcgccagtg ccctgtttgc atggtcagcc catgatgccc
     1741 gccacgtctc ggcatgtgtc cggtcgttcc agtccctgac ctttccgctg aacccagcag
     1801 gtccgatagc cctcgtggtc agcatgatgt gcgcatgggg atttgtttta tccatgtgat
     1861 ggaacgccac atcagcaatc atgccctgac tgacaaagtt ttctctgaca aaggcgagaa
     1921 cggtttcccg tgcggcatcc tgcggcagtt cgtgggggat ggcgagttca atttcccgcg
     1981 caagttgtga gttcttccgg cgttcggcct tctcaacggc attccagagt tcggcacggt
     2041 tcgtacacca ggcaggggcg gtggcggggg tcagaatcac agcctcagcc acaccgcttt
     2101 tgcgggtgta gtcgtgagtc agtccggtac gctcatcaag catcagtgaa ccggaacgat
     2161 aggcagcgga agctacggcg ctgtagccgc tgcttctgga gataattttc atgctgagat
     2221 gataaatcgc catttctgcc gtaatgcctt tttgtagttt tctttcagcc ccgcagggcg
     2281 cacacaatcg aaacggagtg gagattgtat aagtgcgcac ttcgtgattt gccgtgcatt
     2341 atgccagctc agtttttaaa ctaccagtga cgcgctaaaa tgcgattttt catcgcgcac
     2401 ggagttacat catgagcaga acgctcgaac agaagattgc tgatgctgag gcgagattac
     2461 agcgcctgaa ggcgaagagc cggagcctgg acacagcgca gaaggttgtt gtgggtgctg
     2521 cattgctggc aaaggtcaga aagccggagg aggtgcagtt gcgtgcctgg ttactccagt
     2581 tcctgaaggc agaggtgaca cgacaggctg atgtgacgcg catactgccg ctgattaacg
     2641 agctggaggc gctgccagaa cagtgattgt atcgtccgat cgccgcagcc ccagcgaccg
     2701 agcgttagcg agcgagtggg cgaggaagcg gaagtgaatc agtcgccagg ttgagcacat
     2761 acgcacggtt tttcggcagg tgatatgcgc tgaacccgca tgaatactgg gtttccacag
     2821 ggttggaggg ggtgtattcg aaacttgcaa ttctgcttta ttgcaacgca ttgtttttat
     2881 tggttttttg ttttcagcac cccaagttat ccacaggctc gccgttaagt ttttaagatc
     2941 ttatttttag atcttttttt ttaaaacctt tttaggtctt gtaacaccat gattttaaag
     3001 atgttctggc aagataagtg gagcttttgg gggtgaaagg tggagctttt gggggtgaaa
     3061 ggtggagctt ttgggggtga aaggtggagc ttttgggggt gtaatccaca gaaaaagggg
     3121 gttgcaaaaa aaaaacttcc acccatatga taagtggcgt acagtaaaaa aacgccacca
     3181 atagcaacga gcaagcgaaa tgtcagatct gatagcttac aaatcaaacg cccttatcga
     3241 ggcaagctac aagctcactt tgcaggaaca gcgcttcctg ctggtctgta tcagccgcct
     3301 aaaatccgga gaagattccc cttcgccgga tgaacaaaaa accatgacca tcacggctgc
     3361 agagtttcat gatgcctttc ctgacatggg acggcagcac gctgaagcta ggctcaagga
     3421 agcgatagat cgcctctggg acaggtcagt aatcatcaag aacgaggaaa aacgagaaga
     3481 gttccgctgg gtgcagtacc gggcgcagta tttcaaaggt gaggcgaaag tagatatcac
     3541 cttctccgat gccatcatgc cgtatctgac acagctcaag gggcagttta cgcgggtagt
     3601 cgtgaagaac gtctccagcc tttccagcac ctacagcatc cgcatttacg agctgctcca
     3661 gcagttccgc agcaccggtg acagaacgat agctatcgat gatttccgaa ccatgcttgc
     3721 cgttgaagat aaatatcaac aattcaaaga cttgaataag atgataatca agccagccat
     3781 cgccgaactg aacgaaaaaa gcgatctggt tgtaactgtt gagacgatca aaaaggggcg
     3841 cacggttgta gccctgcatt ttcgcttcaa ggaagacaaa cagatcaaga tgacgatcta
     3901 aaatcgattt taaggcacct aaaacgctcc ctgctgcgtt ttttttgtgt tagtagtgcg
     3961 aaggtactat tgaagtgaaa aattgcgtca aaaacgctct gatgcagcga aaaaagcccc
     4021 cccaattttt gggggcggaa aaacaaaact atgcttgcgt tatgtgagtg tgc
//
