LOCUS       NZ_OP242280             5269 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA124 plasmid pLAO61, complete sequence.
ACCESSION   NZ_OP242280
VERSION     NZ_OP242280.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363350
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Sequencing Technology             :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028600645.1-RS_2024_03_23
            Annotation Date                   :: 03/23/2024 03:29:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6
            CDSs (total)                      :: 6
            Genes (coding)                    :: 5
            CDSs (with protein)               :: 5
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242280.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA124"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO61"
     gene            416..616
                     /locus_tag="PUP03_RS00005"
                     /old_locus_tag="OCKDAGFA_00001"
     CDS             416..616
                     /locus_tag="PUP03_RS00005"
                     /old_locus_tag="OCKDAGFA_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_175182663.1"
                     /translation="MARSPDPRQRTEGFRKAEASLRLEGMDPAGTPLYENIKERVISGD
                     ITYEQGRAEILARYQKRANGH"
                     /name="antitoxin VbhA family protein"
     gene            1369..2940
                     /locus_tag="PUP03_RS00010"
                     /old_locus_tag="OCKDAGFA_00002"
     CDS             1369..2940
                     /locus_tag="PUP03_RS00010"
                     /old_locus_tag="OCKDAGFA_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017782005.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_039026336.1"
                     /translation="MTHIACHINTLQLPFVACTVPLTFTLPAAGCTLIGRNGIGKSLLL
                     ELLAGQRRADSGKIEWLTPISWLSQFAPAQDHDCAASVADLIGWGEPLRALERIEQGSC
                     AQDDFELLADRWNLRSDLADLLAEAGLRLDPHVPVSTLSGGQLTRLRLLALFARPAHFL
                     LLDEPDNHLDASGIDWLSQQLARHRGGYLLVSHDRRLLNQTSHILELSEQGLQSARLSF
                     DEFLAQQAEVQAARSTSLAQAVRSEKAAARSLQKVREQAARRASQGRRERGSQAKILLD
                     FKAEQAGKSLGRVLDRHERGHSAQVEARTEAAAALAAVQPLIMQWPEEIERKGAAQPLV
                     ITAASHVRWHGQRVDLVLQRGERCLLGGRNGCGKSSLLQMLRGVLEPETGSFRINGKMA
                     CLDQGLSLIARDLSPLANLLTVAPTLAESDARTRLAGIALRGDRALTPCHGLSGGERLK
                     LGLLMVLAQLPDLLLLDEPDNHLDHPSRQLLTQVLADYPGTLLLVSHDPDFVAGVGVGK
                     ELNLSE"
                     /name="ATP-binding cassette domain-containing protein"
     gene            <3025..3315
                     /locus_tag="PUP03_RS00015"
                     /old_locus_tag="OCKDAGFA_00003"
                     /pseudo=""
     CDS             <3025..3315
                     /locus_tag="PUP03_RS00015"
                     /old_locus_tag="OCKDAGFA_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004661993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /name="type II restriction endonuclease"
     gene            complement(3745..4110)
                     /locus_tag="PUP03_RS00020"
     CDS             complement(3745..4110)
                     /locus_tag="PUP03_RS00020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_175182665.1"
                     /translation="MPPQNRSSSSSSYSSPSVNSGSSSALQIDAAWASFEAMIERRLEE
                     IKPKTPAQIKANGDSRNKTIVISALVVILAIIVIYIGAIYIIDSVTGKEFTSSLMPVIT
                     AIISGVFGYLSGEKSSK"
                     /name="hypothetical protein"
     gene            complement(4110..4664)
                     /locus_tag="PUP03_RS00025"
                     /old_locus_tag="OCKDAGFA_00005"
     CDS             complement(4110..4664)
                     /locus_tag="PUP03_RS00025"
                     /old_locus_tag="OCKDAGFA_00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272954328.1"
                     /translation="MPQIIPLPDEVPPDIPRVAATNMSGTYNFNISLNRLDFTLNVSDS
                     QFKEPEALSDFALKSKLIVKNIPNEIEIIRIGIIGNYFELERLPAIALAKKYSKKDLGN
                     ISEFNFRYNKISQEFGYSFNNVFSISNAEINTKGIVSDGVFIQKDINNNPSGLSIKKDM
                     VMEIITKKIKELSTSCLEGIS"
                     /name="hypothetical protein"
     gene            complement(4961..5110)
                     /locus_tag="PUP03_RS00030"
     CDS             complement(4961..5110)
                     /locus_tag="PUP03_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063923875.1"
                     /translation="MHEPPVQSDCCACPGNYRLESNPERHDKAPLAAATGNGLEELSLD
                     VMRL"
                     /name="hypothetical protein"
     Typing          complement(2..219)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.111"
                     /coverage="93.0"
                     /evalue="7.14e-47"
     Typing          complement(5003..5269)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.654"
                     /coverage="85.0"
                     /evalue="1.44e-122"
ORIGIN
        1 tcctgtcggg tttcgccacc actgatttga gcgtcagatt ctgtgatgct tgtcaggggg
       61 gcgaagccta tggaaaaacg gcttcgccgc ggccttttct gttcccctct ttctccttcc
      121 gctacccccg gatctgtggg taactccagg tggactgccg gtaccgctcg ccgcagccga
      181 acggccgagc gtagcgagtc agtgagcgag gaagcggaag atctgcgatg cctgattggg
      241 ctcgtgtcgc gccgtaccct ctctttaccg ccggattaca cagtttcctg caatactttc
      301 cttccgattt acacattcgt ctgcacggat aaacaacggc agagtacggg caggatatgt
      361 aaagtaggcc tactttaccg cctgccttat tcgcttacag ctcaaaggaa catcgatggc
      421 caggtccccc gatccacgac aacgtactga gggtttccgc aaagcggaag cgtcgctgag
      481 gcttgaaggc atggaccccg ccggaacgcc gctgtacgaa aatatcaagg agcgggttat
      541 ttccggggat atcacttacg aacagggacg ggcagaaatt ctggcgcgtt accagaagcg
      601 tgcgaacggc cactgacacg gacaggaaac taggtaccat cgcttcctca aaaaaacagg
      661 cgtggcctct ctccggccag gcttttcctg tatttgctat ctgtttgcta cttgtttgct
      721 acttgtttgc tacttgtttg ctacctgttt gctatctgtt tgctatctgt ttgctacttg
      781 tttgctaccc tctgctgtga aaagagtaag gcacgttagc gatgtataca tcgccgcgcc
      841 gcaagggttc cccttccgaa tcccgttcat cactgcgtgc tgattatcca cgttaaatcc
      901 gacctcctgc ttggtagggg acggtagaga aaacggacaa aatcgtacgc taagcatgcg
      961 gcctgtcagg ttgaggccat agagtttgac gtttaggcgt cagataatgc agaaagcggc
     1021 cctagtctga cgcgatgcca gtgagccgac tgacgcccag ttgaggtata gcccagactg
     1081 acacttgact tattgataca acatgcaagt gagtgatacc ggaacatttg gcatttttta
     1141 ctatctgaag tatgatttta ctttcatcag taaagagtat ttcgccgatg cgcatttaac
     1201 ctgtccgcac cccacttttg attcttctcc cgccagggag agcgggtgcg tctcaccgtg
     1261 acaggttgat gctgattcct ctgctgtttt cccactagtg gacacgtgtc cctgtcattt
     1321 ggtttagctc ttggcctgcg gcttccgcat tggcctacag gagttcagat gacccatatc
     1381 gcatgtcata tcaacacttt gcagctgccc ttcgtcgcct gtactgtgcc ccttaccttc
     1441 actttgcccg ctgcgggctg caccctgatc ggccgtaacg gcatcggcaa gagcctgctg
     1501 ctggaactgc tggcgggcca gcgccgcgcg gattctggca agatcgagtg gttgaccccc
     1561 atcagctggt tgtcccagtt tgcaccggct caagatcatg actgcgcagc cagtgtggcc
     1621 gaccttatcg gctggggcga gccgctacgg gcccttgagc gcatcgagca ggggagctgc
     1681 gcccaggacg atttcgagtt gctggctgat cgctggaacc tgcgcagcga tcttgccgac
     1741 ctgctggcag aagcaggcct cagactcgat ccccatgtcc cggtcagcac cctgagcggt
     1801 ggccagctca cccgtttgcg cctgctggcg ctgtttgcga ggcctgctca ctttctgttg
     1861 cttgatgagc cagacaacca cctcgatgcc tctggcatcg actggctaag ccagcaactg
     1921 gccagacatc gcggaggtta cctgctggtg agccacgacc ggcgcctgct caatcaaacc
     1981 agtcatattc tggagctgag cgagcagggg ttgcagagtg cccgcctctc gtttgacgag
     2041 tttctggcac agcaggccga ggtacaggct gcccgcagca ccagtttggc gcaagcggtg
     2101 cgaagcgaaa aggcggcggc gcgcagcttg caaaaagtgc gtgaacaggc ggccagacga
     2161 gccagtcagg ggcggcgtga gcgcggctcc caagccaaaa tcctgcttga tttcaaggcg
     2221 gaacaggcag gcaaatccct tggcagagtg ctggaccgcc acgagcgggg ccacagtgcg
     2281 caagtggagg cccgcaccga ggcagcagcc gcgctggcgg cggtgcagcc gctcatcatg
     2341 cagtggccgg aggagatcga gcgcaagggg gctgcccagc cgctggtgat tacggctgcg
     2401 tctcacgtgc gctggcatgg ccagcgggtt gatctggtgc tgcaacgggg tgagcgctgt
     2461 ttgctcggcg gtcgcaatgg ctgcggcaaa tcgagtctgc tgcagatgtt gcgtggggtg
     2521 ctggagcccg agacgggcag ctttcgcatc aatggcaaga tggcttgcct cgatcagggg
     2581 ctcagcctga ttgcgcggga tctcagcccg cttgccaacc tgttgacagt ggctccgaca
     2641 ctggccgaga gtgatgcgag aacacggctg gcgggcatag ccctgcgcgg cgatcgtgct
     2701 ctgacgcctt gtcatggtct gagtgggggg gagaggttaa aactgggtct gctgatggta
     2761 ctggcgcaac tgcccgacct gctgctgctc gatgaaccgg acaaccatct ggatcacccc
     2821 tcgcgtcagt tgctcactca ggtcctggcg gactacccag gcactctgct attggtttct
     2881 cacgatcccg acttcgttgc aggggttggg gttggtaaag aattaaatct ttcagagtga
     2941 ccgcaagatg attgacctag atgcccttgt ggcagggggt tagaggagtt gacggaaatt
     3001 tcggcggttc cggcttacaa ccccgaatac cataacccgg cgttcgactc gctgaagctg
     3061 accatgcttg gtgtgaaatc gacctgcaag gaccgctggc ggcaggttct cgccgaagca
     3121 gaccgcattg atgacaagca tctgctgacg ctggaaacag caatctccac gcatcagacc
     3181 gacgaaatgg cagcaaagcg gctgcaactc gttctgccgc gcagtctgca ccagacctat
     3241 acgccagccc agcaggcatg gttgatggat gttgtcagtt tcacggaact ggtacgtgct
     3301 cgacagaatg cgtgagtgct gaacttcggc ttagcgtacg attttttccg ttttctgagc
     3361 cgaccccttg gtagtaaatt ctgccatgcc tgacggcctg gccaacgcct cacggtcgca
     3421 cagtaaactg tgcacccgtt caccgttatc gggggagtta tgcgccggaa agcaggcttt
     3481 catgccagca tccaccccaa atcgaccgcc attcacctgc gtgcatagcc atgcagccgc
     3541 gcagaaatca tcaggcgcgt tttttcacga tatggacagg gaaagatgcc ggaaataggc
     3601 aggagggcgc agaaatgccc tgagagcctc tcaggcggtg cacgcaacgg atgttatggt
     3661 aaattgaagt taatttcacc aatttgtagt tttgtgtttg gggtaataag tgctgccata
     3721 tcttaataca gcagcgctta ttttttattt ggatgatttc tctccagaaa gatatccaaa
     3781 tacgccactt attattgcgg ttattacggg cataagtgag gacgtgaact ccttcccagt
     3841 gacagaatca attatgtata ttgctccaat atatataact attatagcta atattacaac
     3901 taaagcagat atgacaatgg ttttatttct gctgtcgcca tttgctttga tttgtgctgg
     3961 cgtcttaggt tttatctctt ccagtcttct ttctatcatt gcttcgaaag aagcccatgc
     4021 agcatctatt tgcaaagcag acgagctacc agaattcaca cttggtgaac tgtatgacga
     4081 gctggatgat gaacggttct gtggaggcat taggatatcc cctctaagca acttgttgac
     4141 aactccttga ttttcttagt tattatttcc attaccatgt ctttttttat tgataatcct
     4201 gatggattgt tgtttatgtc tttttgtata aatactccat ctgagactat tcccttagtg
     4261 tttatctctg cattgctaat cgagaaaaca ttattaaaac tatatccgaa ttcctgagat
     4321 attttattgt atcggaaatt aaactcactt atgtttccaa gatctttttt ggagtatttt
     4381 tttgctaagg ctatagctgg aagtctttct aattcgaaat aattgccgat tattccaatc
     4441 cggataatct caatttcgtt aggaatgttt tttacaatta gttttgactt tagtgcaaag
     4501 tcacttaaag cttctggctc tttaaattga gaatcggata catttaatgt gaaatctaat
     4561 ctgttgagtg atatgttaaa gttatatgtt cccgacatat ttgttgctgc cactctaggt
     4621 atatctggtg gtacctcatc tggaagtggg ataatttgag gcatcctatc aaataaattg
     4681 ccagcggcat cattaatact ggaaaaaaaa tgatccggtc ttgttatatg ctgcttaaaa
     4741 aagatagcca actggaaact catgacatgt accatttcaa ctccattttt attagtgtct
     4801 tgactgaagt ataaagcgaa aggtaatctt cttacgaaga aaaaaaaccg ccctggcagg
     4861 cggtttttta gaggttagaa agacctagga aatctaacta accgaggtaa ctggcttaga
     4921 gggagcgcag tcaccaaaac ttgttctttt agtttagcct ttacaggcgc atcacatcaa
     4981 gactcaattc ctctaatccg ttaccagtgg ctgctgccag tggcgctttg tcgtgtcttt
     5041 ccgggttgga ctcaagacga tagttaccgg gacaggcgca gcagtcggac tgaacggggg
     5101 gttcgtgcat acagtccagc ttggagcgaa ctgccttccc ggaaccgagt gtcaggcgtg
     5161 gaatgagaaa ccgcggccat aacagcggaa tgacaccggt aaaccgaatg gcaggaacag
     5221 gagagcgcac gagggagcta ccagggggaa acgcctggga tctttatag
//
