LOCUS       NZ_OP242279             2460 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA118 plasmid pLAO58, complete sequence.
ACCESSION   NZ_OP242279
VERSION     NZ_OP242279.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363349
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600635.1-RS_2024_03_23
            Annotation Date              :: 03/23/2024 03:22:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242279.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA118"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO58"
     gene            complement(join(2101..2460,1..609))
                     /gene="mobV"
                     /locus_tag="PUP18_RS00005"
     CDS             complement(join(2101..2460,1..609))
                     /gene="mobV"
                     /locus_tag="PUP18_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            1282..1785
                     /locus_tag="PUP18_RS00010"
                     /old_locus_tag="MCMIJKKG_00001"
     CDS             1282..1785
                     /locus_tag="PUP18_RS00010"
                     /old_locus_tag="MCMIJKKG_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094320569.1"
                     /translation="MLIHGDRVNTKKISKLERHTYMDESGEIKQDSKIETFSIPAEPPY
                     VKLYIADIAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKN
                     IIAALVDKQIFLRAGRGELEANPFLFARGDWPSIYERRKKIELRISYDSNGRTVQTTVT
                     DDER"
                     /name="hypothetical protein"
     gene            1775..2002
                     /locus_tag="PUP18_RS00015"
     CDS             1775..2002
                     /locus_tag="PUP18_RS00015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022360.1"
                     /translation="MNAEDKLFKKVDRLMLHDSLKKNEVLTVWERTFLSSTYSIYRRTS
                     GNEFSTKGLSPKQKSTAFSILKKYNYHLMD"
                     /name="hypothetical protein"
     Typing          complement(2105..2460)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.639"
                     /coverage="96.0"
                     /evalue="1.6199999999999998e-48"
ORIGIN
        1 cagcgcctcc ggcgagatcg atacctggct acgggtgcca cggtttatcg attcgtagta
       61 gtgctgaact gtctgatgcg tggcgcggct tccctcgatg ccgcgctcaa gacccagttt
      121 ttttactgat tcagcgtagg tgctctgatc ttcacgcatc tttgaccgcc cgccaataaa
      181 ctctttcgcg gacagccgtc catcctgcgt caatggcacc acgaacgcag acaggtgcgg
      241 ggtggcctcg tcccggtgaa ccaccgccgc cacaacacga tccttcccgt atttcttctc
      301 cagccattgc tcagagcggg cgaaaaactc cgcctgctgc cgtggtgtgg cctctttcca
      361 ccactcaggg cttgctgtca taacgtattc aacggccagt accgcatctt tacggcgttt
      421 ttccggcagc agctcccgca cacgccccat tgcctgatcc gttgatttgg acacagagca
      481 gtagttttct ggtgtccgtt cagcgttagc gttcggcgtc tcacgctctc ggtagcagtg
      541 ctgcaacgcg ctggccacgc ttcccattcc cgtcagcttt ttgcagcgca ttatcgcgta
      601 gttcgccatg ttcccccctg attccggcat tcattcagcc aggcggggga gcgcattgcg
      661 atacctccgg cgggccacta tcaagatgat agtgtactca ctagactttg cttcgcaaaa
      721 tcgttcggga agctgcgctt ccaaaggtca aaatcaacgg cggtctggca ggtggaaggc
      781 gtcgctccgc gtcacctccc accactgcca agcctgtttt atccggtgag tcgtcaaggg
      841 taaatggcac tcgctacgct cgcccttgac gaaatcaggc aatgaatatc gtttttgaaa
      901 gagcaggaac cgctcgccgc aagaacggat tagggagcgg catcgccgcg agtctgtccg
      961 ttcgaggaag cggaatttcc actccccgga catagaaatc cgtacgctcc agactcaaaa
     1021 aagctcgttt ttcgatgttt aaaaatggcg tttttcagcc tttattttcc cccttaatat
     1081 atagatatag atacgggaac ccgcgcacac caagggctag agagaagaat cgtactcacc
     1141 tcaagtacta atcgtactta cctcaagtac taatcgtact tacctcaagt actaatcgta
     1201 ctcacctcaa gtactaatcg tactttaggt aaggtacttg actatcggca tctttagtac
     1261 ttaaatgtga gtactaaaaa catgctgata catggggata gagtgaatac aaaaaagatt
     1321 tctaagcttg aacggcatac atacatggac gagagcggag agattaaaca agactctaag
     1381 atcgagactt tttctatccc ggctgaaccg ccctacgtca agctttacat cgcagacatt
     1441 gcgatgattc atggtttgtc aaaatctcaa aatgatgtgc ttttgcagtt ggcaatgctg
     1501 attggatggg atggctttgt atctgtatca aaaactagat ttgagaagac catacaacct
     1561 agggttcata ttgaatatca gacgtttaaa aacataatcg ctgcccttgt tgataaacaa
     1621 atctttctcc gggcagggcg gggagagctt gaagccaatc cgtttctttt tgctcgggga
     1681 gactggcctt caatttacga aagaagaaaa aaaatagagt taagaatcag ttatgattcc
     1741 aatggaagga cagtacaaac aacggtaacg gatgatgaac gctgaagata agctattcaa
     1801 aaaagtagat agattaatgc tgcatgactc tctaaaaaag aatgaggttt tgactgtttg
     1861 ggagcgtact tttttaagta gtacatacag catatatcgc agaacaagcg gtaatgaatt
     1921 ttcaactaag ggattgtcac caaaacaaaa aagcaccgct ttttctattt tgaaaaaata
     1981 caattaccat ctgatggatt agctggactc agcgttatac agggcggcag aacctgatct
     2041 ctggaatgcc gcaaatgacg tcactttgcg gcgttagccc tatcctgcat cgcatcaaaa
     2101 ctaacgctcc ataccaccca tgccacgcga tcgcgtctgt cgctgttttt cacgttcaat
     2161 acgctgctgt ttttcctttt cctggttttg ctgcttaaac cgctgagcca tcatcaggat
     2221 ttcggtcatc tgctctctgg aaagcccctt aaaaggctct gtaacgctct gtaggcgttt
     2281 ctgctgcgca tccatagtct tctgtagctc acgcgctctt tttgcgttct gggcgctctg
     2341 agaggccata gcgatggttc ctgcaaagcc atcgttcact gcgtggctta gacgtttggc
     2401 tatggctgcc tgatcttcca cgtctttggt gaaaatgccc ttccgcaata cacgcggctc
//
