LOCUS       NZ_OP242278             3877 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA118 plasmid pLAO56, complete sequence.
ACCESSION   NZ_OP242278
VERSION     NZ_OP242278.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363349
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600625.1-RS_2024_03_23
            Annotation Date              :: 03/23/2024 03:18:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 4
            CDSs (total)                 :: 4
            Genes (coding)               :: 4
            CDSs (with protein)          :: 4
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242278.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA118"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO56"
     gene            complement(join(3483..3877,1..31))
                     /locus_tag="PUP18_RS00005"
                     /old_locus_tag="GOFFCLNO_00004"
     CDS             complement(join(3483..3877,1..31))
                     /locus_tag="PUP18_RS00005"
                     /old_locus_tag="GOFFCLNO_00004"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_272954447.1"
                     /translation="MTGSGGSPPPETPGIHCETFALGVQNFLSGVGMEKRTKEIKIRLT
                     DDEHQRLLERSQRTHLAEWLRELGLGERTPSKRRKLPDVDPQLFRQIAGTSNNLNQIAR
                     KLNQMESLVAEDKIRLLSLLTSIDRQLTEILEQHRDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(176..832)
                     /locus_tag="PUP18_RS00010"
                     /old_locus_tag="GOFFCLNO_00001"
     CDS             complement(176..832)
                     /locus_tag="PUP18_RS00010"
                     /old_locus_tag="GOFFCLNO_00001"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272954448.1"
                     /translation="MLASCGHYVDGYDGYTKAQLERMDQDIIRYYNKTPVNDGTVFGYN
                     FTKLSNRQYLNQSGFINVISDHKSVDVYEKKNISDGILKTSMIVIKNNKPITLYVAFET
                     LNKGDIPDSFIDAINNLIANNYGVEPIVGTKIKLMTKSDYKYKYIKNRPSKSDYINYKS
                     GISNIIESISYSYDKSKSRYDFNKVVYRIDFVGFAMQQQKYLSAQERSRVDFIGS"
                     /name="hypothetical protein"
     gene            complement(856..1761)
                     /locus_tag="PUP18_RS00015"
                     /old_locus_tag="GOFFCLNO_00002"
     CDS             complement(856..1761)
                     /locus_tag="PUP18_RS00015"
                     /old_locus_tag="GOFFCLNO_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_094322853.1"
                     /translation="MSNVALKLFNDRLPHKPYFTDELQCGLRIAGKERAILAKYIQFNQ
                     PHAQYWLCFDVDRPGAAIDWSDRNAPAPTLTIMNKANGHAHLLYALKTAVRTAPDGKMK
                     PLKYAAAVESALREKLGADVGYSGLICKNPNHENWQIAVWQPELYTLDWLADSLDLNSA
                     NEKTILPDYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLKACYERASAYNLQFISPLD
                     EKEVNGIAKSIANWTYKKFSKVKFEIYVSYTHSSEIQSRRGIASGIKRYIGSLEEQKPW
                     DKLGISRRTYFYKRKKGILK"
                     /name="replication initiation protein"
     gene            complement(1970..3493)
                     /locus_tag="PUP18_RS00020"
                     /old_locus_tag="GOFFCLNO_00003"
     CDS             complement(1970..3493)
                     /locus_tag="PUP18_RS00020"
                     /old_locus_tag="GOFFCLNO_00003"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_239637988.1"
                     /translation="MIVKFNSRGAGAGSGPVDYLLGKERNREQAKTLRGDPERVKTLID
                     TCDFARTYTSGVLSFEEKDLPDVDKQQLMDELEQTLLPGLDKDQYSILWVEHQDKGRLE
                     LNFVIPNIELHSGKRLQPYYDRADRPRVNAWQTLTNDRLGLSDPNDPERRRELTPGRDL
                     PKNSQKAAESITAGLSHLAMQGLVRNRQDVINVLESAGMTIARETKSSISIASPDGGKN
                     LRLKGALYERDFRFSESLRGELEAAGATYRDARESRISEAERCYQRGIELKREEYQQRY
                     PRRDPTTPTALENHLPAGNEMAVDWPVIHRPDLRRTDRGNLLVSEPATRGNTDQRTESS
                     RAEFQNGQGDRGTERDRQIPRSVLHHPAEARQQHTDLPLPETNGSELQRQITQERSPEP
                     LSDDLTHSGKESHDRDRKTLVERIRELTTRLKDTAASLTRQFQEFTGHVRENTARESSL
                     SQSLHEAEPASRGLEQASKRLEQHSESLSQTVAAKELSQLKENDRGMSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
ORIGIN
        1 cggggggcgg cgagccccct gaaccagtca cggtgaaccc tcgttagagg ggactccgtg
       61 tgtactggct tataagtata tttatctact taaaatatca agtcacggaa aaataaaaaa
      121 gccccataaa ggggctgtta tctgatatag gttcgagtgc aaaaagaata gaagtctagc
      181 taccgatgaa atcgactctg gacctttcct gtgcagacaa gtacttctgt tgttgcatag
      241 caaaaccgac aaaatcaatg cgatatacga ctttgttaaa atcatacctg gattttgatt
      301 tatcatagct atatgagatg gattctataa tgttacttat ccctgattta taatttatat
      361 aatcagactt cgatggccta tttttgatat atttatattt gtaatcactt tttgtcatta
      421 atttaatttt ggttccgaca attggttcta caccataatt attagctatt agattattaa
      481 tagcatcaat aaaactatca gggatatctc ctttatttaa agtttcaaaa gccacgtaga
      541 gagtaattgg tttattattt ttaattacga tcatggatgt tttcaaaatg ccatcactaa
      601 tatttttttt ctcgtaaaca tctactgact tatggtcgga aatgacattt ataaaaccag
      661 actgattcaa atattgcctg ttagataact tggtgaaatt ataaccaaaa acagtcccat
      721 cattcactgg ggttttattg taatatctaa tgatatcttg atccatccgc tctagctgag
      781 ccttagtata tccgtcatat ccatcaacat aatgtccgca agatgcgagc ataaatataa
      841 aaggtagtat ttttttcatt ttaatattcc ttttttcctt ttatagaaat aagttctcct
      901 gctaattcct aacttatccc atggtttttg ttcttctaaa ctaccgatat accttttaat
      961 tccggaagca atacctcggc gagattgaat ttctgaagaa tgagtataac tcacataaat
     1021 ttcgaattta actttactaa attttttgta agtccaattc gcaatacttt tagcaattcc
     1081 attaacctct ttttcatcca gtggtgatat aaattgcaga ttataagcac tagctctttc
     1141 ataacaggct tttagccact gctcatactc aggccaacct tgacggatag cgcggtaagc
     1201 ccactttctc gttttatcga acagtgtgca gttacggcct aagccataat cggggaggat
     1261 agttttttcg ttggctgagt tcagatcaag tgagtctgca agccagtcga gggtatacag
     1321 ttctggctgc caaacagcga tttgccagtt ttcgtggttt ggattcttgc aaattaaccc
     1381 cgaatacccc acgtcagcgc ctaatttttc acgcagcgca ctctcaaccg ccgctgcata
     1441 tttgagtggt ttcatttttc catctggtgc cgtgcgtacc gctgttttta gggcgtaaag
     1501 taggtgtgca tggccattgg ctttgttcat gatagtgagt gtgggcgcag gagcgttacg
     1561 gtcgctccaa tcaatggcag cgcccggtct gtctacgtca aagcagagcc agtattgagc
     1621 gtgaggttgg ttaaactgga tatatttggc aaggattgcc cgctcttttc ctgcgatacg
     1681 taaaccgcat tgcagttcgt ccgtgaagta cggtttgtgg ggcaatctgt cgttaaaaag
     1741 tttgagtgct acgttactca atgagccctg cgcttatggc gggctgttgt gttgcacgtc
     1801 tcatgtgcta atatccttct taggttttta cctgcgccct gatttcatat tcccttccgc
     1861 caagttgaga tatgaattcg gggcgttgtc ttttctaatg gcattaaagt tacacgcaaa
     1921 tgacagtttc tgtcgagttc ctcgaataat ttctccgtat accaacaaat tacaggctca
     1981 tccctcggtc attttcttta agctggctca gctctttggc ggctacagtc tggctaagcg
     2041 attcactgtg ttgctcaagt cgtttacttg cttgctcaag ccctcgactt gctggttcag
     2101 cctcgtgcag tgattggcta aggcttgatt ctcttgccgt gttttctcgt acatggcctg
     2161 taaactcctg aaactgtcga gtaaggcttg ctgctgtgtc tttgagtcgt gttgtaagct
     2221 ctctaatgcg ctcaacaaga gtttttctgt ctcggtcatg ggattctttg cctgagtggg
     2281 ttaaatcgtc gctgagaggc tctggtgagc gctcttgggt tatttgacgc tgtaattcac
     2341 taccgtttgt ttctggtagg ggtaagtctg tatgttgctg gcgcgcttcg gcaggatgat
     2401 gtagtactga ccttggtatt tgcctgtccc tttctgtacc acgatcccct tgcccgtttt
     2461 ggaattcagc acggctagac tctgttcgtt ggtctgtatt tcctcgagtt gctggttcag
     2521 ataccagtag gttcccccga tcagtgcggc gcaaatcagg gcgatgaata acaggccaat
     2581 ccacagccat ttcattaccg gccggtaaat ggttttcaag tgctgttggt gttgttgggt
     2641 cgcgtcttgg atagcgctgt tgatactctt cacgcttgag ttcaattcct cgctgatagc
     2701 agcgttcagc ttctgaaatt cggctttcac gtgcgtctcg gtatgttgcg cctgctgctt
     2761 cgagttctcc tcgaaggctt tcgctaaatc ggaaatcccg ctcatagagt gctcctttga
     2821 gtctgaggtt tttaccgccg tccggtgagg cgatgctgat gctggatttg gtttctcggg
     2881 caatggtcat gccggcagat tctaggacgt tgatgacatc ctgacggtta cgaacaagcc
     2941 cttgcatggc gaggtgagag agtcctgccg taatggactc cgccgctttt tggctgtttt
     3001 tcggcaagtc tctgcccggg gtgagttcac gccggcgttc tgggtcgttg gggtcactta
     3061 atccgagtcg gtcattggtc agcgtttgcc atgcattgac ccgagggcgg tcggcacggt
     3121 cgtaataggg ttgtagacgt ttaccgctgt gtaattcgat gttcgggatg acaaaattaa
     3181 gctctaaccg gcctttgtcc tgatgctcaa cccaaaggat gctgtattgg tctttatcaa
     3241 gtccagggag tagcgtttgc tcaagttcgt ccatcagctg ctgtttgtct acgtcaggga
     3301 gatctttctc ttcaaacgag aggacaccag acgtataggt tcgagcgaaa tcgcaggtgt
     3361 ctatcagggt ttttacccgt tcgggatcac cccgtaaggt tttggcctgt tcacgattac
     3421 gttctttgcc gagaagataa tccacgggac cactgccagc acccgcacca cgggaattaa
     3481 atttaacgat cacgatgctg ctccagtatc tcagttaact ggcgatcgat actcgtgagt
     3541 aagctcagta accggatttt gtcctcagcg accagactct ccatctgatt aagtttacga
     3601 gctatctggt taaggttgtt actcgtgccg gctatctgtc ggaataactg cggatcaaca
     3661 tccggtaatt tacgacgctt tgagggtgtc cgttcaccta atccaagttc acgtaaccac
     3721 tccgctagat gggttctctg actccgctca agaagtcgtt gatgttcatc atcggtgagc
     3781 ctgattttga tttctttggt gcgcttttcc ataccgactc cgctgagaaa gttttgcacg
     3841 ccaagtgcga aagtttcgca gtggatgccc ggggttt
//
