LOCUS       NZ_OP242275             3965 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA108 plasmid pLAO53, complete sequence.
ACCESSION   NZ_OP242275
VERSION     NZ_OP242275.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363347
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600595.1-RS_2024_03_23
            Annotation Date              :: 03/23/2024 03:31:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 6
            CDSs (total)                 :: 6
            Genes (coding)               :: 6
            CDSs (with protein)          :: 6
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242275.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA108"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO53"
     gene            complement(join(3310..3965,1..274))
                     /locus_tag="PUP31_RS00005"
     CDS             complement(join(3310..3965,1..274))
                     /locus_tag="PUP31_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_272954305.1"
                     /translation="MIEMDMPNTIHYPHVIPFISQGKINAIKSTFGNNLSDRECYGIYI
                     WSQKASSAIYPLLQQLEVTLRNSIDKEATKLIGQKWWDNVYTDTSKSKHDDFIHNINKA
                     KKRYENEFKKKNPSMANTKIIAPHDDIIAHTDFYTWQAVLSDAFHTQSRSEVSRALWPR
                     LTYRVLKGLDRSKDEGTARIDFLNELNEIRNYRNRLSHNDCIWIKIHSKNLQSAVETIR
                     EKINKIEGLIKTINPQVHLSLTKWGSFYHAKRICSQKEAELHLGKGIINSTTDEMNTIL
                     DQLYALTADGKLTGVVKRNTNNIAFHKF"
                     /name="Abi family protein"
     gene            complement(485..598)
                     /locus_tag="PUP31_RS00010"
     CDS             complement(485..598)
                     /locus_tag="PUP31_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_244749245.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPARHDLSPLAAATGN"
                     /name="replication initiation protein"
     gene            802..957
                     /locus_tag="PUP31_RS00015"
     CDS             802..957
                     /locus_tag="PUP31_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272954307.1"
                     /translation="MSVDFCDARQGGGAYGKTPAALASSGVLLFAHMFFPALSPDSVDN
                     RITAFE"
                     /name="hypothetical protein"
     gene            complement(1094..1288)
                     /locus_tag="PUP31_RS00020"
                     /old_locus_tag="PPBFPJLL_00002"
     CDS             complement(1094..1288)
                     /locus_tag="PUP31_RS00020"
                     /old_locus_tag="PPBFPJLL_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_044788462.1"
                     /translation="MNKQQQTALNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQKSIQTRFESESGTRE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1572..1895
                     /gene="mobA"
                     /locus_tag="PUP31_RS00025"
                     /old_locus_tag="PPBFPJLL_00003"
     CDS             1572..1895
                     /gene="mobA"
                     /locus_tag="PUP31_RS00025"
                     /old_locus_tag="PPBFPJLL_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_001390775.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEIMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            complement(2123..2716)
                     /locus_tag="PUP31_RS00030"
                     /old_locus_tag="PPBFPJLL_00005"
     CDS             complement(2123..2716)
                     /locus_tag="PUP31_RS00030"
                     /old_locus_tag="PPBFPJLL_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_065810797.1"
                     /translation="MSGRKFGRLPNWWARSSWLVNNVRSNEIGVGIASMKCLLALSVLI
                     DFHSRTASVSFTGMERLTGLSRPMIVKGVAKLEKDGLIKIDREMFVNSYELTVQPNDDR
                     WAKVPVDTIRKKLNTISNRGMAPFVALKLYLTIISLRYQSNNTVKVTHETLRSYTGVQP
                     NQIRFGLDVLYCSRLIHLQPKEDRESNIYEIIGL"
                     /name="replication protein"
     Typing          1326..1563
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.49"
                     /coverage="99.0"
                     /evalue="9.98e-112"
     Typing          complement(527..1052)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.35"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          1573..1892
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.131"
                     /coverage="100.0"
                     /evalue="3.68e-62"
ORIGIN
        1 tttttgatgt gtcggtgtat acattatccc accatttttg gcctattagt tttgtcgctt
       61 ctttatcaat tgagtttctc aaagtaactt cgagttgttg caataatgga tatatggcag
      121 atgaggcttt ctggctccat atatatatcc cgtagcactc cctatcagac aggttattac
      181 caaacgtgct ttttatagca tttatttttc cttgggatat aaatggtatt acatgagggt
      241 aatgaattgt atttggcata tccatctcta tcatacactt agtggtgatt ggatgttgcc
      301 atacacgtat ggatagttgt agagtgttaa acgaaaaaac cgccttggcg ggcggttttt
      361 cgaaggttca aagagttggc gctctgtgaa ccaaggtaac tggcttggag gagcgcagtc
      421 accaaaatct gttctttcag tttagcctta acaggtgcat aacttcaaga caaactcctc
      481 taaatcagtt accagtggct gctgccagtg gcgataagtc gtgtcttgcc gggttggact
      541 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      601 agcccagctt ggagcgaacg acctacacca agccgagata ccaacagcgt gagctatgag
      661 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagt ggcagggtcg
      721 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      781 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      841 gcctatggaa aaacgcctgc ggcgctggct tcttccggtg ttttgctttt tgctcacatg
      901 ttctttccgg ctttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgaact
      961 gacaccgctc gccgcagccg aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa
     1021 gagcgcctta tgtggcattt tctccttacg caccggtata ccgttctgca tcctgtctgg
     1081 tgtaggaaca ctgttattcc ctggtgccac tttccgattc aaagcgtgtc tgtatgcttt
     1141 tctgaagttc ttccgcgagt tcgtgcagct tctcacacat ggcggcctgt tcgtcagcat
     1201 caagtgcgtc cagtttttcg agcagcgtca ggctctggct ttttatgaat cctgccatgt
     1261 tgagtgcggt ttgctgctgc ttgttcatct ttctgttttc tccgttctgt ctgttatctg
     1321 cctcgtgtga ttatatcgcg taccactttt cgactgtttt gccgccggta ttctgctgtc
     1381 tgactcgttg acgggtattt ctgcctgaca atactgtcac tgccaagaaa ctgccgtgcc
     1441 tttgtcggta attcgaactt gctgacagga caggatgtgt aattgttata ccgcgcatac
     1501 atgcacgcta ttacaattac cctggtcagg gctctgcccc gacacccatg tcagatacgg
     1561 agccatgttt tatgacaaaa cgaagtggaa gtaatacgcg caggcgggct atcagtcgcc
     1621 ctgttcgtct gacggcagaa gaagaccagg aaatcagaaa aagggctgct gaatgcggca
     1681 agaccgtttc cggtttttta cgggcggcag ctctcggtaa gaaagttaac tcactgactg
     1741 atgatcgggt actgaaagaa attatgagac tgggggcgtt acagaaaaaa ctctttatcg
     1801 acggcaagcg tgtcggggac agggaatatg cggaggtgct gatcgctatt acggagtatc
     1861 accgtgccct gttatccagg cttatggcag attagcttcc cggggagaaa actgtcgaaa
     1921 acagacggta tgaacgccgt aagcccccaa accgatcgcc attcactttc atgcatagct
     1981 atgcagtgag ctgaaagcga tcctgacgca tttttccggt ttaccccggg gaaaacatct
     2041 ctttttgcgg tgtctgcgtc agaatcgcgt tcagcgcgtt ttggcggtgc gcgtaatgcg
     2101 acgttatggt aaatgcattt cctcataggc cgattatttc atagatgttt gactctctat
     2161 cttcttttgg ttgcagatgt atcaggcgac tacagtacag tacatctaag ccgaatctaa
     2221 tctggtttgg ctggacacct gtatagcttc tcagtgtttc atgtgtcacc ttaacagtat
     2281 tatttgactg ataacgaagg cttataattg ttaaatatag ttttaatgcc acaaatgggg
     2341 ccatccccct gttcgatatg gtgtttagtt ttttcctgat agtatctact ggtacttttg
     2401 cccacctatc atcatttggc tgtactgtta attcatagga gttaacaaac atctctctat
     2461 ctatctttat aagaccatct ttctcaagtt tggctactcc tttcactatc atggggcggc
     2521 ttagtccagt cagtctttcc attccggtaa atgatactga ggctgttctt gaatggaaat
     2581 caatcaagac ggatagagct aacaagcatt tcattgatgc aatgccgacc cctatctcat
     2641 ttgaacgaac attgtttaca agccatgagc ttcgggccca ccagtttgga agcctgccaa
     2701 attttctacc gctcattttt accccatttt cggtcaagga gagatataaa tagctttatt
     2761 atcatataca tgaaaaccat tgcacctaaa gatacaacag aaaccaaaac ctccggcttt
     2821 gcggaagtta gcgttaccag caaattgttg agggtttcca attggtcctc cagtcgcata
     2881 ctaagttgtt gtgttttaac tttatatacg cttattagaa taaatctaga taaagcaata
     2941 tttggtgact gtttgttttt tcatataaaa catagtgtta tcttgttttt ctcgtttttt
     3001 aactggtaaa tgtaaatttg ataatgctgg tttaatcggc gtttttagtt atttttgttg
     3061 tgtgtttttt ggtctattgg taaatggtgg atggtaggct ttagcgtgaa catgggaaat
     3121 agtttgctct ttttcctgat gatgatgtat catgtatggc agtgaagcct tgagctttcg
     3181 ctttaatgca ttgtgaaatt tttatagatt cacaatgaca ttaaggccac gctccaccga
     3241 aaggtgataa tggcaagaga tttggatgag gctcgacagg aaactgtcgg gccttttcta
     3301 ttttgtgatc taaaacttgt gaaatgcgat attgttggta tttcgtttta caacacccgt
     3361 tagcttccca tcagcagtta gtgcgtatag ttgatctaga attgtgttca tctcatctgt
     3421 tgtgctattg attattcctt tgcctagatg caattcggcc tctttttgag aacatattct
     3481 tttggcgtga taaaaggaac cccacttcgt taatgataga tgtacctgtg ggtttatcgt
     3541 ttttatcagt ccttctattt tgtttatttt ttctcttatt gtttctactg cagattgcaa
     3601 atttttagag tgtattttta tccatatgca atcattatgc gataacctat ttctgtagtt
     3661 tctgatctca ttcagctcgt ttaagaaatc aattctggct gtgccttcat ctttgcttct
     3721 atctaatccc ttcagtactc tgtaggtgag cctgggccat aaggctcttg atacttcaga
     3781 tcttgattgt gtatgaaaag catcgctcaa aaccgcttgc catgtgtaaa aatctgtgtg
     3841 tgcaatgatg tcatcatgag gtgcaattat tttggtattt gccattgatg gatttttttt
     3901 cttgaattca ttctcatatc ttttttttgc tttgtttata ttatgtatga agtcatcgtg
     3961 tttgc
//
