LOCUS       NZ_OP242273             2043 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA243 plasmid pLAO51, complete sequence.
ACCESSION   NZ_OP242273
VERSION     NZ_OP242273.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363371
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600575.1-RS_2024_03_23
            Annotation Date              :: 03/23/2024 03:14:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 2
            CDSs (total)                 :: 2
            Genes (coding)               :: 2
            CDSs (with protein)          :: 2
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242273.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA243"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO51"
     gene            join(1474..2043,1..399)
                     /gene="mobV"
                     /locus_tag="PUP17_RS00005"
     CDS             join(1474..2043,1..399)
                     /gene="mobV"
                     /locus_tag="PUP17_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            complement(461..895)
                     /locus_tag="PUP17_RS00010"
                     /old_locus_tag="PBLHJEGM_00002"
     CDS             complement(461..895)
                     /locus_tag="PUP17_RS00010"
                     /old_locus_tag="PBLHJEGM_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004193480.1"
                     /translation="MSNRITQIDTATGEEVGSFVAVIRPKQKSAFERHFTMNQDALKIL
                     AKTLTGEQFKVLMMLLADLDYENYIQIAQADIADALGMQKTNVSRSVKALIDLSVILQG
                     PKVGRSYSYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="helix-turn-helix domain-containing protein"
     Typing          2..396
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.212"
                     /coverage="96.0"
                     /evalue="7.52e-58"
ORIGIN
        1 ggcacccgta gccaggtatc gatctcgccg gaggcgctgg agccgcgtgt attgcggaag
       61 ggcattttca ccaaagacgt ggaagatcag gcagccatag ccaaacgtct aagccacgca
      121 gtgaacgatg gctttgcagg aaccatcgct atggcctctc agagcgccca gaacgcaaaa
      181 agagcgcgtg agctacagaa gactatggat gcgcagcaga aacgcctaca gagcgtcaca
      241 gagcctttta aggggctttc cagagagcag atgaccgaaa tcctgatgat ggctcagcgg
      301 tttaagcagc aaaaccagga aaaggaaaaa cagcagcgta ttgaacgtga aaaacagcga
      361 cagacgcgat cgcgtggcat gggtggtatg gagcgttagt tttgatgcga tgcaggatag
      421 ggctaacgcc gcaaagtgac gtcatttgcg gcattccaga ttaggttctg ccgccctgta
      481 taacgctgag gccgtttttt agtgctttct tgtggttgct gacagtacct ttccagccaa
      541 actgagggtt cagacggtaa ctgtaactgc gccctacttt tgggccttga agaatcacgg
      601 ataggtcaat tagcgcctta accgaacggc ttacattggt tttctgcata cctaaagcgt
      661 cggcaatatc ggcttgggca atttggatgt aattttcata atcgagatca gctaacagca
      721 tcatcaagac tttgaattgc tcccctgtga gggtcttagc aaggatcttt aaagcgtctt
      781 ggttcatagt gaaatgcctc tcaaaagctg acttctgttt gggtcggata actgcgacaa
      841 aactacctac ctcttcccct gtagcagtgt ctatctgagt aattcggtta ctcatttaag
      901 aggctctccc aaaaaaatag ttatcaataa tgataacaac gttatcaata atgataacta
      961 tcgtcaagcg agagccgcgc cccacaaggg ctgagagaag gctctcttct gtatcttata
     1021 gcgagaagct catatgcccg gatagcgaac cctattttca tcggttcgtg cgtaagcatt
     1081 tctatgcccg ttcaggtgat attccgcttc ctcgttcagg ctgttcgttc ctcgctgcgc
     1141 tgcggtacgc ctcagcctga cctgcggcga gcggttcctg ctctttcaaa gacgatttca
     1201 ttgcctgatt tcgtcaaggg cgagcgtagc gagtgccatt tacccttgac gacactccgg
     1261 ataaaacagg cttggcagtg gtggggggtg gcgcgaagcg acgccttcca cctgccagac
     1321 cgccgttgat tttgaccttc tggcagcttg ctgcccgaac gattttgcgt agcaaagtct
     1381 agtgagtaca ctatcatctt gatagtggcc cgccggaggt atcgcaatgc gctcccccgc
     1441 ctggctgaat gaatgccgga atcagggggg aacatggcga actacgcgat aatgcgctgc
     1501 aaaaagctga cgggaatggg aagcgtggcc agcgcgttgc agcactgcta ccgagagcgt
     1561 gagacgccga acgctaacgc tgaacggaca ccagaaaact actgctctgt gtccaaatca
     1621 acggatcagg caatggggcg tgtgcgggag ctgctgccgg aaaaacgccg taaagatgcg
     1681 gtactggccg ttgaatacgt tatgacagca agccctgagt ggtggaaaga ggccacacca
     1741 cggcagcagg cggagttttt cgcccgctct gagcaatggc tggagaagaa atacgggaag
     1801 gatcgtgttg tggcggcggt ggttcaccgg gacgaggcca ccccgcacct gtctgcgttc
     1861 gtggtgccat tgacgcagga tggacggctg tccgcgaaag agtttattgg cgggcggtca
     1921 aagatgcgtg aagatcagag cacctacgct gaatcagtaa aaaaactggg tcttgagcgc
     1981 ggcatcgagg gaagccgcgc cacgcatcag acagttcagc actactacga atcgataaac
     2041 cgt
//
