LOCUS       NZ_OP242272             2621 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA104 plasmid pLAO50, complete sequence.
ACCESSION   NZ_OP242272
VERSION     NZ_OP242272.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363346
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600565.1-RS_2024_03_23
            Annotation Date              :: 03/23/2024 03:21:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 2
            CDSs (total)                 :: 2
            Genes (coding)               :: 2
            CDSs (with protein)          :: 2
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242272.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA104"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO50"
     gene            complement(67..804)
                     /locus_tag="PUP21_RS00005"
                     /old_locus_tag="OEFILJDI_00001"
     CDS             complement(67..804)
                     /locus_tag="PUP21_RS00005"
                     /old_locus_tag="OEFILJDI_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198901125.1"
                     /translation="MPYYALREKEVLKLFKRNNLSSSIAAIKIYILICLTSNLDENGNY
                     CTSLTYDQLTKYASLSRKLVSNGLKKLYELELLSFEGDKKKKYYLMGVKRTGKVFSETR
                     FKSSQGYWAKLPFAGFVDGSGRITAFESMSNRSPVELNSLKLFIYLLSIRSKDQVSISV
                     TLSKIRSKLDISFQDIMIAIGFMQSIGMLYKVNLGVNTISNYDAAFSINFLVCGWESLE
                     WKPTYLSHDEWKNKMLAKMFPEK"
                     /name="hypothetical protein"
     gene            complement(1579..1779)
                     /locus_tag="PUP21_RS00010"
     CDS             complement(1579..1779)
                     /locus_tag="PUP21_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_057527577.1"
                     /translation="MLDDRTRQLRVDGFRKAEASLRLEGMDPSGTPLYESVKARILSGE
                     ITYGEGLAEILAHYQKRADSN"
                     /name="antitoxin VbhA family protein"
     Typing          1831..2451
                     /name="rep_cluster_2324"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.605"
                     /coverage="94.0"
                     /evalue="0.0"
ORIGIN
        1 ctttaaaatg gttagcggtc aagaagtcag gcttccttct aagctcaaga ttagtcaaaa
       61 aaaacatcat ttttcaggaa acatttttgc taacatctta tttttccatt catcatgaga
      121 aagataagtg ggcttccact ccagactctc ccatccgcaa acaagaaaat ttatggaaaa
      181 ggcagcatcg tagttagata tagtgttaac gccgagatta accttataaa gcatgcctat
      241 tgactgcatg aaacctatcg caatcattat atcctggaat gaaatatcta actttgatct
      301 tatcttactt aacgtcacac taatagaaac ctgatctttt gatctgatag ataacaaata
      361 aataaaaagc tttagtgaat ttagctcgac tggacttcga ttgctcattg actcaaatgc
      421 tgtaattcta ccagaaccat ctacaaaccc agcaaacggc agttttgccc agtatccttg
      481 tgatgacttg aatctggttt ctgaaaaaac cttcccagtt ctcttcacac ccatcaaata
      541 atattttttc tttttgtcac cctcaaaact cagcaactcc aattcatata gttttttcaa
      601 accattgctg accaacttac ggctaagcga ggcgtacttg gtcaattggt cataagttaa
      661 agaggtacaa tagttcccat tttcatctaa gttgctagtt aagcaaatta aaatatatat
      721 tttaatggca gcaatgcttg aagataaatt attcctcttg aaaagtttta aaacttcttt
      781 ttctcgaaga gcgtaataag gcatgggctt ccaaaagggc gtcatttatc ctccaaaata
      841 catgtgtaga caaatcagca gcatgaccac tgacagaatc agccgcgata aatccatcga
      901 gatacgagca atgcgctcaa gtaaagctac ccagttttca ggtgacataa tcaatctccc
      961 agtggtcatg aatgcgtttt ttaaaattat gaccgagaac cctaaagact tatagtcttt
     1021 agggttaaat ctttctagaa taagcgcaca ctaaaaaatc attattatca gtacattgta
     1081 taaaaataat agttcatgtt aataccagaa tagtgcacgt aatccatttt atagcagcat
     1141 catgcctgag tgatcttcat atcagagtcc tatttaccat aacatccctt gcccgcaccc
     1201 ccagaaaggc cctcagcgcg tttctgccgc atccgggccg ttttgggcac ctttccctgc
     1261 ctgtatcgtg aaaaaacgcc cctgatgatt ttggcgcggc tgcatggcta tgcacacgag
     1321 tgaatggtcg ggggtttcgc gcggaagctg tcgccaaagg gggatttccg gtgattaact
     1381 cacccgataa cggtgaacgg gtgcacagtt tactgtgcgg tcgtgaggcg ttggccaagc
     1441 cgccaggcgc ggcaggaata tcgccaaagg ggtcggattt aacgaggagg aaccggagta
     1501 cctggcgggt gtcgggcagg agcctgacca ggaggatttg gcgtgccggc ggtactccaa
     1561 agacacatct tgtccgcgtc agttgctgtc agcgcgcttc tggtaatgag ccagtatttc
     1621 agcgaggcct tctccatagg taatctcacc ggaaagaata cgtgccttaa ccgattcata
     1681 gagcggcgtt ccagaggggt ccatgccttc aagccggaga gaagcctcgg ccttacggaa
     1741 tccgtctaca cgaagttgtc gtgtacgatc atcgagcatg ggttttcctt tgagcagtca
     1801 tgcgaataag gcaggcgtaa agttagctaa ctttacatgt cctgcccgga tattgccgtt
     1861 gtaccgttgt gcagggaaaa gtggaaaacg acaagaaacc gttgcagccg agtgtgtaat
     1921 cagggtggaa acgggtgtac cgagcgatcc gagccctgtt cagcattaca gacattacgc
     1981 ttcctcggtc attcgctcac ttcgttcggt catgacgctg cggcgagcgt tacttgctca
     2041 ggcagaaggc catgacggag agtgaaagag agctgagacg ttgccggttt tccataggct
     2101 tcgccctcct gacgagactc acagaaatca ggcgatttct ggtgctcaaa tcagaagtgg
     2161 cgaaacccga caggacttaa agatccccac cgtttccgat gggtcgctcc ctcgtgcgct
     2221 ctcctgttcc caccctgagg tttacccgtt gcagaccgcc gtaaggtggc gttttctcat
     2281 tcctacccgt gcaactgggt tccttgtagg tggttcgctc caagctgggc tgtatgcacg
     2341 aactccccgt tcaacccgac taccgcgcct attcaggtaa ctgtactctt gaatccaacc
     2401 ctgacagaca cgacaaagcg ccactggcgg tagccactgg taacaggttt agaggaggag
     2461 agtcttgaag tttcgggtgc gataaggcta cactgaaagg acagttttgg tgactgtgct
     2521 cctccaagcc agttaccgcg gttcaaaaga gcgtcaactc ttgcgaacct tcgaaaaaac
     2581 caccttgccg ggtggttttt tcgtttcaag gctaagatac g
//
