LOCUS       NZ_OP242266             4072 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA084 plasmid pLAO46, complete sequence.
ACCESSION   NZ_OP242266
VERSION     NZ_OP242266.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363383
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600505.1-RS_2024_03_23
            Annotation Date              :: 03/23/2024 03:17:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242266.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA084"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO46"
     gene            join(3914..4072,1..96)
                     /gene="mobC"
                     /locus_tag="PUP15_RS00005"
     CDS             join(3914..4072,1..96)
                     /gene="mobC"
                     /locus_tag="PUP15_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            628..1320
                     /locus_tag="PUP15_RS00010"
                     /old_locus_tag="ANPBHKDM_00001"
     CDS             628..1320
                     /locus_tag="PUP15_RS00010"
                     /old_locus_tag="ANPBHKDM_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281403.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1576..3735)
                     /gene="mobQ"
                     /locus_tag="PUP15_RS00015"
                     /old_locus_tag="ANPBHKDM_00002"
     CDS             complement(1576..3735)
                     /gene="mobQ"
                     /locus_tag="PUP15_RS00015"
                     /old_locus_tag="ANPBHKDM_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029353.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     Typing          complement(3378..3736)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          3737..3913
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          629..1320
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1580..3822)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.332"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttactccagt tcctgaaggc agaggtgaca cgacaggctg atgtgacgcg catactgccg
       61 ctgattaacg agctggaggc gctgccagga cagtgattgt atcgtccgct cgccgcagcc
      121 ccagcgaccg agcgttagcg agcgagtggg cgaggaagcg gaagtgaatc cgtcgccagg
      181 ttgagcacat acgcacggtt tttcggtagg tgatatgcgc tgaacccgca tgaatactgg
      241 atttccacag ggttggaagg ggagtattcg gaacttgcaa ttctgctttc ttgcaacaca
      301 ttgtttttat tggttttttg ttttcagcgc cccaagttat ccacaggctc gccgttaagt
      361 ttttagatct tgtttttaag atcttgtttt taatacttgt tttagggtca aaattcatta
      421 ataaaatcaa tatgttatag aggtatatgt ggaggaattt aaggtaaagg tggaggaatt
      481 taaggtgttt aggtggagga atttaaggta aaggtggagg aatttaaggt gttattcaca
      541 gaaatgtgag gatataaaaa aaaatattcc acatttctta tttgtggggc acattaaagc
      601 atcaccacat ttgaacggga tctttgtatg tcggacttga ttgcctacaa gtcgaatgct
      661 ttggttgagg ccagctacaa gttgacccta caggaacaac gttttctgtt gctgtgtatc
      721 agtcgattga aatctggtgc tgatgctgaa ttgcaaaaga caatgaccat taccgctgcg
      781 gaatattttg attcattccc tgacatgggg cggaaaaatg ccgaggttca gttacaggaa
      841 gcgatcgatc gtttatggga caggtcgatc attctcaaga atgacgagaa gcgtgaggag
      901 ttccgctgga tccagtatcg ggcacagtac gctaaaggtg aggccaaggc tcgaataacc
      961 ttttctgacg cggtaatgcc ttatctgacg caactgaagg ggcaattcac gcgcgttgtt
     1021 ataaaaaaca tatcaggttt atcaagtagt tacagtataa gaatatacga gttcctccag
     1081 caattccgca gtacgggaga gagaattatt gctcttaatg actttcgttc aatgttaggg
     1141 attgaaaata aatacaaaca attcagagac ttaaataaaa tactaataaa accctgtgtt
     1201 gacgaactga acaaaaaaag cgacctggct gtaacggttg agacgatcaa aaaggggcgc
     1261 acggttgtag ccctgcattt tcgcttcaag gaagacaaac agatcaagat gacgatctaa
     1321 aatcgatttt aaggcaccta aaacgctccc tgctgcgttt tttttgtgtt agtagtgcga
     1381 aggtactatt gaagtgaaaa attgcgtcaa aagcgctcta atgcagcgaa aaaagaaccc
     1441 ccaatttttg ggggcggaaa aacaaaacta tgcttgcatt atgtgtgtgc ttcttgcatt
     1501 catccctgtc gatgaacata gaagaacatc gacgaacata acacgatgtt aaacaaaaca
     1561 aaatattgtt cacattcact gtgaccaacc gcgatcatgt ctcctctcgg tggtgtgtgt
     1621 tcttgcatct tcctcgcgcc gccattcatc ccaggtacga ttgaaaccca gagagcggaa
     1681 cagatgatcc ggctgttttt ctggtgtgtt gacggcagca aaaagcggtg cctcacgggt
     1741 tttcagatga aactccataa gttccggcgc aggtgatatg tggctgtctg catcgtgatt
     1801 tacggcaaca ccataccctg cataccgtaa ccccttatcg gtcagtgcac tgatgaccag
     1861 ctggctgtta tcggctgagg gaacggcaaa cgcgggatac agcgtaccat taagccggac
     1921 aacaccgccc agagagtcat ccacgcccat gaccagcaaa agctgaagct ggcgggtttc
     1981 ccggtcgaca gaactcatta ccggcatcag cgtcggtagc agacgtccct gcgtaaaatc
     2041 caccttcctt gctgccagtg atggcatggc tggctggcgg tcttctccaa gacagaaaga
     2101 ctccggccat gtcttcggct ggcggagatt gcgggtatat tgcggccatg tctggcaaac
     2161 ctgcgtcaga agttgctcaa aggtcgcaga ggaataaaca cgttgcagtg gtggatgttg
     2221 accgatttcg cttcggtcac agatataacc actcctgtca cgcattaaat caacacgctc
     2281 aaagattctg gtgcggtatt tctccagctc gttcccccag agcgtgtatt ctccgttccg
     2341 gttgtgaagc agaaggtaac aggtcatttc cccgttacca ggcatggggg cctcgccata
     2401 ccccgtaacg actgcgctca gtacccctga atccataacg gcattgtcat ttttcctgag
     2461 ctgttccgct cgtcgctccg cgcgactacg ttctgtatcc tcctgctcct tccggcgcaa
     2521 ttcctcttca cgtttaagca actggactct gagagctgca aggccggatg ccccccactg
     2581 ctgccactgt ttttcaaatg ccgtctgact cgcgggaatg tcctgtttct ccaccttccg
     2641 gtaatgctgt cgcaattttt ccatttgctg gctcagcgac tggatttttc cctgttcgct
     2701 acggtattcc ggtgccgccc attgcatcag gggaatacgg gaaaaaccgg acgaaaacgg
     2761 cgatgatttt ttcaggctgg cagcatggct catcgcttcc tttcgttcgg tgttcagctc
     2821 ttcccagcgt ctgcgcgtcg cctgcatatc tgccatcatc gacatggcat tactggtgag
     2881 tttccatgcc gcattactgt ccttcggcaa cgcttcgatg atgtcacgta gtgtgtacga
     2941 agttgcgttg tcaggaagca cgatattcag aaggtcagcc acacggcggg caggctctgt
     3001 cagcccgctg ttcctgagtt cagtgcggga aatctgtatt tccaggttaa gcctgttaat
     3061 cagacgattc tgttcgccgc gttctgtttc gataccgcgt gtttccatcg cacaggcaga
     3121 ctttccgagg tgaataccag gcgttttctc aagcccctgt cgctcgtatg aacgatggtc
     3181 tatctcttcc tgataaccgg cgttcgccag tgctctgttt gcatggtcag cccatgatgc
     3241 acgccacgtc tcggcatgtg tccggtcgtt ccagtccctg acctttccgc cgaacccagc
     3301 aggtccgaca gccctcgtgg tcagcatgat gtgcgcatgg ggatttgttt tatccatgtg
     3361 atggaacgcc acatcagcaa tcatgccctg actgacaaag ttttctctga caaaggcgag
     3421 aacggtttcc cgtgcggcat cctgcggcag ttcacggggg atagcgagtt caatttcccg
     3481 cgcaagttgt gagttcttcc ggcattcggc cttctcaacg gcattccaga gttcggcacg
     3541 gttcgtacac caggcagggg ctgtcgcggg ggtcagaatc acagcttcag ccacaccgct
     3601 tttgcgggtg tagtcgtgag tcagtccggt acgctcatca agcatcagtg aaccggaacg
     3661 ataggcagcg gaagctacgg cgctgtagcc gctgcttctg gagataattt tcatgctgag
     3721 atgataaatc gccatttctg ccgtaatgcc tttttgtagt tttctttcag ccccgcaggg
     3781 cgcacacaat cgaaacggag tggagattgt ataagtgcgc actttgtgat ttgtcgcgca
     3841 ttatgccagc tcagttttta aactaccagt gacgcgctaa aatgcgattt ttcatcgcgc
     3901 acggagttac atcatgagca gaacgctcga acagaagatt gctgatgctg aggcgagatt
     3961 acagcgcctg aaggcgaaga gccggagcct ggacacagcg cagaaggttg ttgtgggtgc
     4021 agcattgctg gcaaaggtca gaaagccgga ggaggtgcag ttgcgtgcct gg
//
