LOCUS       NZ_OP242265             7463 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA084 plasmid pLAO45, complete sequence.
ACCESSION   NZ_OP242265
VERSION     NZ_OP242265.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363383
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7463)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7463)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600495.1-RS_2024_03_23
            Annotation Date              :: 03/23/2024 03:17:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 8
            CDSs (total)                 :: 8
            Genes (coding)               :: 8
            CDSs (with protein)          :: 8
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242265.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA084"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO45"
     gene            364..585
                     /locus_tag="PUP15_RS00005"
     CDS             364..585
                     /locus_tag="PUP15_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144240.1"
                     /translation="MTKLDENIPHIIDAAVDFMASSQAFREYLKKLPPDNTIPPDLTDE
                     HVPLYIRRLEYYRLLYRPKQTESYWKKR"
                     /name="hypothetical protein"
     gene            complement(639..1172)
                     /locus_tag="PUP15_RS00010"
                     /old_locus_tag="GIKLAALL_00001"
     CDS             complement(639..1172)
                     /locus_tag="PUP15_RS00010"
                     /old_locus_tag="GIKLAALL_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_011666803.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAIEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(1175..2101)
                     /locus_tag="PUP15_RS00015"
                     /old_locus_tag="GIKLAALL_00002"
     CDS             complement(1175..2101)
                     /locus_tag="PUP15_RS00015"
                     /old_locus_tag="GIKLAALL_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_113227874.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDGVSRENRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2278..2676
                     /locus_tag="PUP15_RS00020"
                     /old_locus_tag="GIKLAALL_00003"
     CDS             2278..2676
                     /locus_tag="PUP15_RS00020"
                     /old_locus_tag="GIKLAALL_00003"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016249654.1"
                     /translation="MRISNVEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQENERKQTIQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQELKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(2870..3805)
                     /locus_tag="PUP15_RS00025"
                     /old_locus_tag="GIKLAALL_00004"
     CDS             complement(2870..3805)
                     /locus_tag="PUP15_RS00025"
                     /old_locus_tag="GIKLAALL_00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_011666805.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDKRFMAL
                     CMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
                     /name="replication initiation protein"
     gene            4178..4624
                     /locus_tag="PUP15_RS00030"
                     /old_locus_tag="GIKLAALL_00005"
     CDS             4178..4624
                     /locus_tag="PUP15_RS00030"
                     /old_locus_tag="GIKLAALL_00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_137508464.1"
                     /translation="MEGSARRFSVSELHVSPGQKKTDQGSGQESESVRNFFSCGMRQCS
                     QPPQNTLRLIKNNWHYPVPDKKTTKVGNPRETIGVRLSQNIDNEIVTQINLAASLILIQ
                     YSIKNARIINHIYKDIYQTKQPSSQLRIRIFLYVIDKTQFFYKL"
                     /name="hypothetical protein"
     gene            complement(5117..6775)
                     /locus_tag="PUP15_RS00035"
                     /old_locus_tag="GIKLAALL_00006"
     CDS             complement(5117..6775)
                     /locus_tag="PUP15_RS00035"
                     /old_locus_tag="GIKLAALL_00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007372656.1"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_001529697.1"
                     /translation="MKIVIVGGVAGGASAAARARRLSEDVSIVVFERGSDVSFANCGLP
                     YHIGGKIPLRQSLILKTPEDFKSRFNIDVRIRHEVMSVDPVNKMVTVKNLTSGEVYTEE
                     WDRLLLSTGAAPVVPPLPGLQEAGVFTLRNLTDMDAIQAWIEQHNIAHTTLVGGGFIGL
                     EVMEALSERGISVTLLEMGEQVMAPVDPEMASALHQEIRSHGVDLRLRTALTEVLRTET
                     GFRVALSDGGFLQTDMVILAIGVKPESSLATGAGLAVGKRGGISVNACMQTSIPDIYAV
                     GDAVETPDLVFQEPANVPLAGPANRQGRIAADNMLDRHSLYHGSQGTSICKVFSLSIGS
                     VGANEKQLKAHGTRYEKVYVHAADHASYYPGATMISLKLLFSPDTGKILGAQASGKKGV
                     DKRIDVLAVVQRAGLTVNDLEHLELTYAPPFNSARDVVNQAGMVAANVIKGDTVICHVR
                     DVLQREPGSYCLLDIRSPAELKQFGEYPDALSIPLDSLRENLDKLPEDKEIIIGCQSGL
                     RGHVAYRMLQSHGFRTYNLSGGFITWQAVTESMSQ"
                     /name="FAD-dependent oxidoreductase"
     gene            6936..7283
                     /locus_tag="PUP15_RS00040"
                     /old_locus_tag="GIKLAALL_00007"
     CDS             6936..7283
                     /locus_tag="PUP15_RS00040"
                     /old_locus_tag="GIKLAALL_00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329508.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_023144239.1"
                     /translation="MPMNEDTKRQTDMKNAALGAADVLRGLANSDRLLLMCQLSQGEAN
                     VTELEAAVGITQPTLSQQLGVMRRLKLVETRRAGKMVYYRIGDNRIMTLLNTLYDLYCP
                     QADPDKVKKQP"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     Typing          complement(2871..3805)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.902"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1179..2101)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.403"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agaggggata aggaatatga atactccggt caggatatta tcctagcttt tatatacaac
       61 atgctcacaa ggacaacaac tagctagtta atagatatta aaaattttag agctgaaacc
      121 ttaaagcttg cttccattcc atgttttatt ataaacctta tcttgaaaac tgaaaggaag
      181 tacggcagat taattaagtt gcgtgcattc ttttcacgtt ataatacaaa caagaacacc
      241 tgcgaagtta tttctcctta catatccccc tataccaacg ggggattttt tctggttcac
      301 taacgcataa ataatagtga catactataa ttcacgaacc gtgataatat cagatcaatc
      361 atcatgacaa aattagatga aaatatccca catattatcg atgcagctgt agattttatg
      421 gcatcaagcc aggcatttcg tgagtatctg aaaaaactcc cccctgataa cacaataccg
      481 ccagatctta ctgatgagca cgtaccgtta tatatacgaa ggctggaata ctatcgactg
      541 ctttatcgac caaagcagac agaatcatat tggaaaaaac ggtgaacggt tcgcgaaatc
      601 ctttcgcgct ccgtgaggcg ttggacaagc gaagcgcgtc agtttttccg gctcttcccc
      661 gtgacaggtt tctccaccag tttatcttcc agtgtcacaa ctttctgata caccgccgct
      721 gtggcgttgt agttcctgtc gagtattgtt ttcattcccc atccgagcac acagaacata
      781 accagaaaag ccactgacac cccacccagc catttccagc tatacccttt aagggtgttg
      841 tattcctgca cgatattgct gtgaattgtc gcaatatccc tgttcagggc atccgcgttt
      901 gccttcatgt ttgcgactga ctcttccagc cgctgaaaat cctggctgat gcgctcacct
      961 atcagtcctg ccacgtccag cttctgcacc tcgtttctga cttcatccct gacctgtttt
     1021 gccacaccgt cagaaatatc ccgatatccg cgaagagttt cccttgccac agccagagaa
     1081 tcagcaacag ctttctgttg ctctattgcg gtatgttgca gatgctccac cgataacagg
     1141 agcgtgagta atttttcttc tgtgctggcc atgttcagaa tcccatagaa aatccacgtg
     1201 acggtgattt aacccgttca ggttcagttc ggttctctct gctcactccg tctctcatgt
     1261 caaagccatg ctcccgcgca acatcatgtt gcagccttct gaccagttca tgttgcttaa
     1321 ccggtgtatc cagccattcc ctgatgtcat gcccgaccat tcgcgcctgt tcaagatggc
     1381 gggaatagtc cggttcagac cgtttttgca tgatgtcatg acgctgggca tcatcaagtc
     1441 gtccggcagc ttcaaaaccc agatgcactt cgggacgtcg ttcaatgccc tgttctttca
     1501 gtgtccggca gtcaatgaag cccctttcaa agccatgctt tctcatatgt tcgttgtgct
     1561 tcacctccca gcgttttcgc aattcccgca actgctcttt ttgctgagtg ggagtcaggc
     1621 ttccggaatc ctttttcgca cctccccgct cgggatacct ggcgttatag cgccggaaat
     1681 actgttccgg tgtacggtca atgccatcat tcacacgctg actcatcatg atatgcgcat
     1741 gaggctgttc gccgccgtca atgctcgctt tcggattgtg aatggcaaaa ctccaggcgt
     1801 gccgttcgcc ggcttcctga cgtacaaaat cctgcaccag ttcaaggcgc tgttccggtg
     1861 tcagttcacg gggtaatgca atttcaagtt ccctataggt cgaaccgttc gcacgttcaa
     1921 actggtcagc cgcctgccag aaatgggaag gattatccct ggcccattcc ggcatgtttc
     1981 cgtgtgcgga atattccaaa tcctgacggt cgcggtattt ctctttgcgc tcgatgtaat
     2041 cggcgtggtt tgcagcctgc cccttgccac caaatttaac gctgaggtga taggttgcca
     2101 cggtttcagc cctccgcagg agcgcactgt ttctcgacga aggagagaaa catataagtg
     2161 cgcttccttc taattattcg aattagcacg gaagataccc cctgcacccg ccgaccgcaa
     2221 gcgatatact gcacgaccac aaaccgccgt aaaaaaacac cagggagtac cacaaacatg
     2281 cgcatcagca acgtcgaatg gctgaagaaa cggatcggat tcatcaggaa gctcggagaa
     2341 cagaccgcgc gacagagaca gattattgac ctgctcgaca acgaagccgg actcacagaa
     2401 caggaacgca aacttctcca cgtgctggca acagcggaaa aaaacgactt acaggcacag
     2461 gagaacgagc gaaaacagac gatccagaaa cgaatcgagg gaaaaaaaca acgacgggaa
     2521 cgcaatcacc gactctttct cgcggctggg ctactgatcg aagcaggact ggtagacaca
     2581 aaaacaggcg agctatgcta taaaaaagac aggatattac aggaacttaa ggaaattaag
     2641 tacgatctgg agacatcccc aaacccagac gcgtaagtgc ctccatccgt gttccggttc
     2701 cctggtgctt cctcgctcat tcgctcgcta cgctcggtca tgagactgcg gcgagcacta
     2761 ccagcacaat catgcggaaa acagcaccat tgcagggctg aacggggaaa aacggcaaaa
     2821 gacctgctgc tcaaaagaac gccagaaagg cgctgttatc tgatataggc tattttccgc
     2881 ttttccagtt tcgaatggtt cgagtagaaa cctccaacat cgccgcaata gctttctgag
     2941 aatatccatt ctccagcata catagcgcca tgaaacgctt gtcatcgtag gcttcgcctt
     3001 tagcgatgcc acctttcctg cctctcttgg cctgtatttc tggcgtgtgc gtatcagcca
     3061 catactgtgc gaaagtttcc ggcgtgaagt tcctgtgcgt gtatttcgca atactcttgc
     3121 caatagcccg acattcagga ggtgaaagcg gaacgggaag cgatgcgttg tacatttcga
     3181 cacgctgaat cacggcatca agccactgtg agaatgctgg ccagccctga cgaatcgccc
     3241 tgtaagccca tttgcgcgtc atttcgaaca ggtggcagtt tcgccccatc ccgtaatgtt
     3301 tatcgatgct acgacgcgct gaggcgctca aatcgagata atcagccagt tcatcgaggg
     3361 tataggcttc ctcgcgccac tccatcacca gccattccag gtggaacgga tttttgcaaa
     3421 tcaggccgct gtagttcaca tccgcacaaa gtttctcaca cagcgaacgt tcaaccgctg
     3481 cggcatattt cagcgcctta accgaggcat caggcgcggt tctcacggcg atgttgagtg
     3541 cgtagagcag gtgagcgtgt ccgttcacag gatttttaac ggtgatgttc ggcgcgggag
     3601 cgttccggtc gctccagtca atcgcagccc cctcgcggtc aacgtcaaaa accagccaga
     3661 actgtgcatg aggctggttc tgctggatat accgtgcaag aagcgcgcgc cctttgccgg
     3721 agatacgaag gccgaaagcg agatcatccg tgtgataggg acggtggggt aaattttctt
     3781 cgaagtattg aagcgcggcg ctcatgtgtt acgattcctt ccaggttatc gaccgacatc
     3841 gcccctgtat tttcgtgaaa gtcttggcgg atagacacga atttcagggg cgtttgtttt
     3901 tcagtcgctt acactacacc caacggcaaa tcctgtcgat ccttcccgaa aaaccgatcc
     3961 atttaccata aatcccgttg tacgcccccc ttaaaacgcc ctcagtcgct ttctggcgca
     4021 aaaaacgcaa aaagacaaga aaacaccagg caaaaccaga aaaacgcgtc agaatcgttt
     4081 tcagggcgtt gcataactat gcacgaaagc gatgtgcgat cggtttcgtg cgttctgaac
     4141 accgaacggc tcaaaacgaa aagttttctc cccccggatg gaaggttctg cccggagatt
     4201 ttctgtttca gagcttcacg tttcgccagg acaaaaaaaa accgaccaag ggtcggggca
     4261 agaatcagag tctgtgagaa atttttttag ttgtggaatg cgtcaatgtt cgcaaccgcc
     4321 ccaaaatacc ctgaggctga taaaaaacaa ctggcactat cccgttccgg ataaaaaaac
     4381 cacaaaagtg gggaatccaa gagaaacgat aggtgtcaga ttgagtcaga atattgacaa
     4441 tgaaatagtt acgcaaataa atttagctgc atcacttatt ctgattcaat actcaataaa
     4501 aaatgcccgc ataataaacc acatttacaa agatatatat caaacgaaac aaccaagttc
     4561 acagctcagg ataagaattt ttctctatgt aatagataaa acacagtttt tttataaact
     4621 gtaatatatg agctgtaata ttcatgtatt aattgttttt ttgaaaagca tcacaaattt
     4681 attttttacg catactgccc tattgcaacc agcaatggtt ttcacttatt ttcaacatct
     4741 catttaatac gagttatact tcaacccgtt taaagtgcaa cactaactga gtatcatttg
     4801 ttttccttac ctacccctgg attcccaggg gttttttata actacaaacc agcacaacaa
     4861 aaaaaacaaa acacaataaa aacaatgaat tatataaaac tcaacgatat ctttgtacgg
     4921 agatttacat tcaggaacat tggggtcagt ttggatatag agaattattg tacggtaagc
     4981 ctgttttttg aacagtccac attatcgcaa tagggtacca tccagggtgt taattatttt
     5041 tgtagatttc tattccatat aatacgtttt caatgaatct ggaagatgag gtgccatgtg
     5101 tatggcacct catctcttat tgactcattg attccgtaac cgcctgccaa gttataaagc
     5161 caccactcag attatatgtc cggaagccgt gagactgaag catacggtat gccacatgcc
     5221 cccgcagacc actctgacac ccgataataa tctctttatc ttcaggcaac ttatcaagat
     5281 tctcccgcag ggaatcaaga gggatggata gcgcgtcagg atattcacca aattgtttca
     5341 gttctgcagg agaacgaata tcaagtaaac aataactgcc cggttcccgt tgtaacacat
     5401 cgcggacatg acagatgacc gtatcgccct tgataacatt tgccgccacc attccagcct
     5461 ggttaacgac gtcccttgca ctgttgaaag gcggagcata ggtcagctcc agatgctcga
     5521 gatcgttaac ggtaagcccc gcgcgctgta cgacagccag gacatcgatg cgtttatcaa
     5581 cccctttttt ccctgacgcc tgggccccga gaattttacc ggtatcgggg ctgaacagca
     5641 gtttcagact gatcattgtt gcccccgggt agtaactggc atgatcagcg gcatggacgt
     5701 aaaccttctc gtatcgggta ccatgggctt tcagctgctt ttcattcgcc ccgacactgc
     5761 caatactcag cgaaaatacc ttacaaatgg atgtcccctg actgccatgg taaaggctgt
     5821 gacgatcgag catattatca gcagcaatgc gtccctggcg gttggctggc cctgccagag
     5881 gaacattagc gggttcctga aagacaaggt caggtgtttc aactgcatcc cccacggcat
     5941 agatgtcagg gatacttgtc tgcatacagg cattgacact tatgcctccc cgtttaccca
     6001 cggcgagccc ggctcctgtg gcaaggctgc tctcaggttt taccccgatg gcgagaataa
     6061 ccatgtcggt ctgcagaaac ccaccgtcag acagtgcaac gcggaatccc gtctctgtcc
     6121 gcagtacctc ggtgagcgcc gttctgagac gcagatccac gccatgactc ctgatttcct
     6181 gatgcagggc cgatgccatt tcaggatcaa ccggagccat aacctgttcc cccatctcca
     6241 gcagggtcac actgatcccc ctttcgctca gtgcctccat cacttcgagc ccgataaatc
     6301 cccccccgac aagcgtggtg tgagcgatat tatgttgctc aatccacgcc tggatagcat
     6361 ccatgtcagt aagatttcgc agtgtaaaga cacccgcttc ctgtagtccg ggcaaggggg
     6421 gaacaaccgg agcggcaccg gtactgagga gcagacgatc ccattcttcg gtatagacct
     6481 ccccggaggt cagattttta accgtcacca tcttattaac cgggtctact gacatcactt
     6541 catgacggat acggacgtct atgttaaaac gggatttaaa atcctccggg gttttcagaa
     6601 tcagcgactg ccgtaacgga atttttccgc ctatatgata aggcaatcca cagttggcga
     6661 aagagacatc gcttccccgc tcaaacacaa caatactgac atcctctgat aaccgacgag
     6721 ccctggctgc agcggatgct cccccggcaa caccaccaac aatcactatt ttcattacat
     6781 cctctctgtt cactcggggc aaaatatctg ccatatataa ttagttaccc ggtccagctg
     6841 gacgctgcag acctgaagat cacggtggca aaattacgcc actatcatta tactgaacaa
     6901 tattatataa aaatacataa tataagaaac cagcaatgcc catgaatgaa gacacaaaac
     6961 gtcagactga tatgaaaaat gcagccctgg gggcagcaga tgtcctacgg ggactggcca
     7021 acagcgaccg gctattgttg atgtgtcagt taagccaggg ggaagcgaat gtgaccgagc
     7081 tggaagcggc ggtcggtatc actcagccga ccctgtctca gcagttgggt gtcatgcgtc
     7141 gcctgaagct ggtcgagacc cgacgggcag gtaaaatggt atattaccgt atcggggata
     7201 accgcatcat gacgttattg aataccttat atgatctcta ctgcccgcag gcggaccccg
     7261 acaaggtcaa aaaacaacct taacgtacaa taattttcga tatccaaact gcccccaaca
     7321 ttcaccagca cagttagaca ttaacaatgc aaaatcaatg cgattccggt gccgtatgtc
     7381 agggagaatt atgtaaacgg gggggggccg gaatcgcatt ccagctcata tcattaaata
     7441 cagaaaaaaa cctctgagaa cac
//
