LOCUS       NZ_OP242264             1934 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA061 plasmid pLAO35, complete sequence.
ACCESSION   NZ_OP242264
VERSION     NZ_OP242264.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363341
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600485.1-RS_2024_03_23
            Annotation Date              :: 03/23/2024 03:22:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 2
            CDSs (total)                 :: 2
            Genes (coding)               :: 2
            CDSs (with protein)          :: 2
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242264.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA061"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO35"
     gene            602..904
                     /locus_tag="PUP16_RS00005"
     CDS             602..904
                     /locus_tag="PUP16_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150476.1"
                     /translation="MAGSIRARSLYLYLRDNSGDKKWHQALRTKASGLICVVEGFGSIL
                     LGVVALKATPATTAIVRGCDHLQKFDSKRQPTGDTAMITRQMSHRPAWDGVHAKP"
                     /name="hypothetical protein"
     gene            891..1877
                     /locus_tag="PUP16_RS00010"
                     /old_locus_tag="HPDBGDDL_00002"
     CDS             891..1877
                     /locus_tag="PUP16_RS00010"
                     /old_locus_tag="HPDBGDDL_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_016245711.1"
                     /translation="MQNPKLQNLTDYSPSDAPWDAHKSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGETLNRMNMAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPSMFTRDYVKHDRWVELWRECLRVNYDPNVDVRAVKPRKPKDGE
                     SLASATAELVRGAVAETLKYSTKPADMVADPEWFLELTKQTHKRRFVATGGALKDILKL
                     DQETDADMVIGDDIPEGDDDGSRIAFEWKTESKKYRRSPSKDKAESD"
                     /name="protein rep"
     Typing          complement(1604..1792)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.947"
                     /coverage="98.0"
                     /evalue="1.39e-59"
ORIGIN
        1 cggtactcca aaaacacatc ttgtcctatt ggttctactc cgatgcagga tagggctaac
       61 gccgcaaagt gacgtcactc aattactgag ttcggtttcc gaactcagtt gggacaagat
      121 gtgtttttgg tacaccgcca tcacgatgtc ggtgccaaaa acgattcgtc agggctgcgc
      181 cccgacaccc tgccaacacc tcacgcagtc agattaaaat ctgccagcgc tcgctgtttc
      241 cgaaatcgtc aaactctaaa tcgtggggtc ccgctggctt gccagtgggt tccgatttag
      301 tgattgacga cattgctgat tgaaaaattg gctgaggtgg ttggattcac gaagtgaggc
      361 caaccacctg gaagacggtt tttaattgtc tggtaccgga tacaaccaga acgaatggag
      421 ttggttctgt cgtgatgcga aaaagtatgg gttcgcaaat cacagactaa atagatttac
      481 ccaattccat tcgagtgagg ttgttatgtt ttttgaggtt cttaatcatg ttgatagtgc
      541 gttgagcgtt gctgtttcgc tcctggcgtt gtggcagttt ttggtggcgg gtgggcgttg
      601 aatggctggt tcgatccgcg cgcgatcctt gtatttatac ttaagggata actctggcga
      661 taaaaaatgg catcaagcct tgcgtaccaa ggcttcgggg cttatttgcg ttgtagaagg
      721 ttttggaagt attctgttgg gagtggtcgc cttaaaagcg acccccgcaa cgacggccat
      781 cgttcggggg tgtgaccacc tgcagaaatt cgattcgaag cgacaaccaa caggtgatac
      841 ggcaatgata acacgtcaaa tgagccatcg cccagcatgg gacggtgtgc atgcaaaacc
      901 ctaaactcca gaacctcacg gattactccc cctcagacgc gccctgggac gctcacaaaa
      961 gcgtttctga cgacgtaggt gggatttacc ttctcgcagc cgaatatgag cgctacgggg
     1021 cgcgtatggc ttcctgtggc ggtttactga ggtttggttg gtctaccctc aaagaaactg
     1081 gagaaactcg ccttaggcta cgtgaggcgc atttctgtcg tgtccggcac tgtcctgtct
     1141 gccagtggag gcgatccctg atgtggcaag cccgttttta tcagtccctg ccgcgtattg
     1201 ttgcggatta tccagatgca cgctggatgt tcctgaccct cacagtgcga aactgcgcga
     1261 tcggggaatt gggtgagaca cttaaccgaa tgaacatggc cttccagcga cttaaggatc
     1321 gcaaagagtt tcgacctgtt cagggctgga tccgcaccac ggaagttaca cgtggaagtg
     1381 acggtagtgc gcacccgcac tttcacactc tgatgatggt tcctccctca atgttcacgc
     1441 gagattacgt taaacatgac cgctgggtag agctttggcg agagtgtttg cgtgtgaatt
     1501 acgatccgaa tgttgatgtt cgagcagtaa aaccccgcaa gcctaaggac ggagaatcat
     1561 tggcttctgc aactgcggaa cttgtacggg gcgcagtggc tgaaaccctg aaatattcca
     1621 cgaagccggc tgacatggtt gctgatcctg aatggttcct cgaattgact aagcagacac
     1681 ataagcgccg atttgtggct accggtggcg cactcaaaga catactgaaa ctcgatcagg
     1741 aaactgatgc cgatatggta atcggggatg atattcccga aggcgatgat gatggttcac
     1801 gcattgcttt tgagtggaaa accgaatcca aaaagtaccg tcgctcaccg tcaaaagata
     1861 aagctgaatc agactgacag ggtgtcgggg cgcagccctg accacgacgt ttttgagagg
     1921 acgccgcttg tcgg
//
