LOCUS       NZ_OP242259             2042 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA041 plasmid pLAO26, complete sequence.
ACCESSION   NZ_OP242259
VERSION     NZ_OP242259.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363379
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600435.1-RS_2024_03_23
            Annotation Date              :: 03/23/2024 03:12:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 2
            CDSs (total)                 :: 2
            Genes (coding)               :: 2
            CDSs (with protein)          :: 2
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242259.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA041"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO26"
     gene            368..1336
                     /gene="mobV"
                     /locus_tag="PUP12_RS00005"
                     /old_locus_tag="ICIBFNKB_00001"
     CDS             368..1336
                     /gene="mobV"
                     /locus_tag="PUP12_RS00005"
                     /old_locus_tag="ICIBFNKB_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_272954385.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNADAERTPENYCS
                     VSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWNEATPRQQAEFFARSEQWLE
                     NKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMRDDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     VAKRLSQAVNDGFAGTVAMASQSAQNAKRARELQKTMDSQQKRLQSVTEPFKGFSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMSR"
                     /name="mobV"
     gene            complement(1400..1831)
                     /locus_tag="PUP12_RS00010"
                     /old_locus_tag="ICIBFNKB_00002"
     CDS             complement(1400..1831)
                     /locus_tag="PUP12_RS00010"
                     /old_locus_tag="ICIBFNKB_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="WP_024183523.1"
                     /translation="MRRITQVDQSTGEELGGFVAVIRPKQKSSFQRHFTMNQAALITIA
                     NELNHDQMRVLMALLAELDYENYIQVAQIDIAEALTMQKTNVSRAVKNLIDFGIILEGP
                     KIGRSKTYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="lipoprotein"
     Typing          321..1333
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.746"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttccgcttcc tcgaacggac agactcgcgg cgatgccgct ccctaatccg ttcttgcggc
       61 gagcggttcc tgctctttca aaaacgatat tcattgcctg atttcgtcag gggcgagcga
      121 agcgagtgcc atttaccctt gacgactcac cggataaaac aggcttggca gtggtggggg
      181 gtggcgcgga gcgacgcctt ccacctgcca gactgccgtt gattttgacc ttctggcagc
      241 ttgctgcccg aacgattttg cgtagcaaag tctagtgagt acactatcat cctgatagtg
      301 gcccgccgga ggcatcgcaa tgcgatcccc cgactggctg gatgtacgcc agaacgaggg
      361 ggtaagcatg gcgaattacg cgataatgcg ctgcaaaaag ctgacgggaa tgggaagcgt
      421 ggccagcgct ttgcagcact gctacaggga gcgtgagacg ccgaatgctg acgctgaacg
      481 gacaccagaa aactactgct ctgtgtctca gtcggcagat gaggcgatgg gaaagctgcg
      541 ggagctgttg ccggaaaaac gccgtaaaga tgcggtactg gctgttgaat acgttatgac
      601 ggcaagccct gagtggtgga acgaggccac accgcggcag caggcggagt ttttcgcccg
      661 ttctgagcaa tggctggaga acaaatacgg gaaagatcgc gttgtggcgg cggtggttca
      721 cagggacgag gccaccccgc acctgtctgc gttcgtggtg ccattgacgc aggatgggcg
      781 gctgtccgcg aaagagttta ttggcgggcg gtcaaagatg cgtgacgatc agagcaccta
      841 cgctgaatcg gtaaaaaaac tgggtcttga gcgcggtatc gagggaagcc gtgccacgca
      901 tcagacagtt cagcactact acgaatcgat aaaccgtggc acccgtagcc aggtatcgat
      961 ctcaccggag gcgctggagc cgcgtgtact gcggaagggt attttcacca aagacgtgga
     1021 agatcaggca gccgtagcca aacgtttaag ccaggcagtg aacgatggct ttgcaggaac
     1081 cgtcgctatg gcctctcaga gcgcccagaa tgcaaaaaga gcgcgcgagc tacagaagac
     1141 tatggattcg cagcagaaac gcctacagag cgttacagag ccttttaagg gcttttccag
     1201 agagcagatg accgaaatcc tgatgatggc tcagcggttt aagcagcaaa accaggaaaa
     1261 ggaaaaacag cagcgtattg aacgtgaaaa acagcgacag acgcgatcgc gtggtatggg
     1321 tggtatgagt cgttagtttt gatgcgatgc aggataggtc tgacgccgca aaacgaggtc
     1381 atttgcggca ttccagagat caggttctgc cgccctgtat aacgctgagg ccgtttttta
     1441 gtgctttctt gtggttgctg acagtacctt tccagccgaa ttggggattt agccggtacg
     1501 ttttgctccg cccaattttc ggtccttcca gtatgatccc gaaatcaatc aggtttttta
     1561 ccgcacgact gacatttgtt ttttgcatag tcagcgcttc cgcaatatct atctgcgcaa
     1621 cttggatgta gttttcgtaa tccagttctg cgagcaaggc catcagcaca cgcatctggt
     1681 catggtttag ctcattggca atggtaatta gcgcggcttg gttcatagta aagtgcctct
     1741 ggaatgatga cttttgttta ggacgaatca ctgcgacaaa accacctaat tcctcccctg
     1801 tactttggtc tacctgcgtg attctccgca tctaaaaatc tccattcggg aaaaaatagt
     1861 tatctctcaa gataactaag ttatctctag agataactat tgtcaaaaac tttccgcttg
     1921 tggcgcggct tccgagagat ccccttctgt atcttattac tctccgtact catacatcgg
     1981 caaaatcagg tgtttttctg ccagtattga gtgtccgaat ttctatgtgc ggggagtgga
     2041 aa
//
