LOCUS       NZ_OP242257             5631 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA041 plasmid pLAO24, complete sequence.
ACCESSION   NZ_OP242257
VERSION     NZ_OP242257.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363379
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Sequencing Technology             :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028600415.1-RS_2024_03_23
            Annotation Date                   :: 03/23/2024 03:14:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7
            CDSs (total)                      :: 7
            Genes (coding)                    :: 6
            CDSs (with protein)               :: 6
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242257.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA041"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO24"
     gene            274..1278
                     /locus_tag="PUP12_RS00005"
                     /old_locus_tag="MPNCDEMM_00001"
     CDS             274..1278
                     /locus_tag="PUP12_RS00005"
                     /old_locus_tag="MPNCDEMM_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272955173.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRILGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSF"
                     /name="hypothetical protein"
     gene            1319..2194
                     /locus_tag="PUP12_RS00010"
                     /old_locus_tag="MPNCDEMM_00002"
     CDS             1319..2194
                     /locus_tag="PUP12_RS00010"
                     /old_locus_tag="MPNCDEMM_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_000609294.1"
                     /translation="MINMIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSA
                     VILSWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLES
                     VFILVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLS
                     AAFYVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGIL
                     QYFLAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSV
                     YFLNRKYSEE"
                     /name="DMT family transporter"
     gene            complement(3397..3588)
                     /locus_tag="PUP12_RS00015"
                     /old_locus_tag="MPNCDEMM_00003"
     CDS             complement(3397..3588)
                     /locus_tag="PUP12_RS00015"
                     /old_locus_tag="MPNCDEMM_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3653..3976
                     /locus_tag="PUP12_RS00020"
                     /old_locus_tag="MPNCDEMM_00004"
     CDS             3653..3976
                     /locus_tag="PUP12_RS00020"
                     /old_locus_tag="MPNCDEMM_00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4185..>4616
                     /locus_tag="PUP12_RS00025"
                     /pseudo=""
     CDS             4185..>4616
                     /locus_tag="PUP12_RS00025"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4654..5184
                     /locus_tag="PUP12_RS00030"
     CDS             4654..5184
                     /locus_tag="PUP12_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            5191..5424
                     /locus_tag="PUP12_RS00035"
     CDS             5191..5424
                     /locus_tag="PUP12_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137437.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          3222..3303
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          2578..2765
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          3967..5528
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcgcggcag tttttcgggt ggtttgttgc catttttacc tgtctgctgc cgtgatcgca
       61 ccgaacgcgt tttggcggtg cgtacaatgc gacgttatgg taaatggagc ggattttcag
      121 gaaggagtga tactaacaac tcgaacactg tgcaaataat gattttttta aaatatcggt
      181 aacgataata aattatttcg ttttacgccg gtaataaata taatgtagtt tcagtatata
      241 aaaaaatata tgaggtgtct tgtgggagct ttcatgaatc ttcaacaacg tataaataaa
      301 ctaccacaat tatcatccag tttttcattt ggaaaagata ttgataatat tcattcattt
      361 atctttaacg aaacttctaa agataaaatt gaagatttac taagaaaatg ggtgtcaggc
      421 aatcagccct gtgtatttgg taaattggca agcaagaaaa ttaaagggct tgatttccat
      481 ttatctattg taaatagccc ccaactatat aatgatgatg gtcatttgtt tgattttttg
      541 cgtaatgaaa gagttcgttt taaagaacgc gcaagaagag gagaggtcag tgcgcatctc
      601 atttatttta ttcatccaca acttgccttt gctcgtccat cagaagagtt agtggacata
      661 caaaaatata tttgttcgct gcatatgcca gagtgctatc caataaaaga ggatgttatt
      721 tatactgaaa gcgtaccatt tcaggataaa gatgggctaa aaatatataa ggctggtgtt
      781 aatgtttttt actcttctgc tcatagaaca agaaatcatg atagaagaat actaggggga
      841 atacttattt ctgtaaatgc cccggggcat tttatgaggc tggcgataga aaaaggtttc
      901 tataaagatc aagaacaagc gttagcggat attcgtaaca tgactattca gtctgttggt
      961 aagggtgggt attcacatcc ggaagggatt tctacaacat ggcattcaga atctaatttg
     1021 gatcgttttg gatgcccagt gcacaccggt aatagttctt attacagcgg tttttatcat
     1081 actgatgtat taattccagg agaattaact aaggatgaaa gattgctcca tgaaatagaa
     1141 aatagtgatc caatgatctt caactggaat gtattgtttt atgtttcatt agaagagttt
     1201 cctatagatg atccttatta tggagagttt attggtattc ctgtagatga tgcatctatg
     1261 ttttttaact ccttttaacc aaggaaattt gaaaacaaac ccctttatga gaaagaggat
     1321 gattaatatg attaaaggaa tgctttttgc ttttttagca gcgttctcat ggggggcagc
     1381 tatcgtaatg agcaaaaaag gagttgaaga actagatgcc ggaggggtgt ttttctggca
     1441 gatttgtagt gcagtaatac tatcttggat agttcttttt ataaaaagaa aaaaactccc
     1501 attaaactca aaagcgatac ttgcttatgc gacaggcgtt tttgaaccat tcttagcgta
     1561 tacttttaca ctctacggac tttattttat ttcagcagga agcgcatcaa ttattttttc
     1621 acttgaatca gtgtttatat tggtgttgtc tgttcttatt tgctcagtta aaattaattc
     1681 cccgcaatat tttatattgt tccttatagg tgcaattgtt ggcagtattt tggtagcctt
     1741 gccagacatc agtaatggat cgaataatat tgttggatat tgtcttgttg ttgccggtgt
     1801 gttatcggca gcattttatg ttgttatatc gtctcgcctt attaaatcgt ttgagcctat
     1861 cacattattg agcggacagt tgacattctc ccttatactg tcatcgctct ttatttttat
     1921 atctaaatca tcaatttatc ttccgttgga atccgtgatt attgtttttt catctggaat
     1981 attacaatat tttttagctt tttgttttta cttgaactcc ttgcgttggc tcccagttca
     2041 tttggcaggg ataatgttgt actttattcc gctagttgca ttatttttgt catggttgtt
     2101 tttggatgaa caattcacat tcttacaggg ggtaggattt attgtaactg tgctaagtgt
     2161 atattttctt aacagaaaat actcagagga gtaacattct gttcaggatt gccccccggc
     2221 ctccatcgat accgtgccaa atgcacgtaa aaaacgggag cgtttctggt gtcggaatgt
     2281 aaccaagggg gtcagaggcg cgctaaaacg ctctcagggg agtcaaatag agacgtttgt
     2341 aacaaaaaga tcaaaggatc ctcttgagat cctttttttc tgcgcgtaat ctgctgcctg
     2401 taaacgaaaa aaccgccttg cagggcggtt tgatcgaagg ttaagtcagc ttgcagactg
     2461 cttaaccagg taactgtctt atgcgagacg agtcaccaaa attgttcttc tagtgtagcc
     2521 gcactccggc agccacttca agtactctct aaatatctcg cacatgactt ttaccaatgg
     2581 ctgctgccag tggcgctttg tggtgtcttt tccgggttgg acccaagaga acagttaccg
     2641 gagaaggcgc agcagtcggg ctgaacgggg ggttcctgca tacagcccag cccggagcga
     2701 actgcctaag tcgccccgag ataccagaag acgtgagcta tgagaaagcg ccacgtgtcc
     2761 cgtaagggag aaaggcggaa aaggtatccg gtaatcgata tggcgggaac gtgggggcgc
     2821 aggagggagc gattcaccgg aaacggtgat gatctttaag ccacgtcggg tttcgccacc
     2881 gctggcggat ttcatggttg agccgcaagg ctcccacaga tgcaccgaaa aagtgtctgt
     2941 tttgtgaagt ccgtcagggg ggcggagcct atggaaaaag cgcctccggc gcggcctttc
     3001 ccggggtttc gttttttgcg cacatgttct ttcctggttt atccccgggt tctgtggata
     3061 acagggttag cgtttttgag aaaggagagt ccgctcgccg cagccgaacg accgagcgta
     3121 gcgagtcagt gagcgaggaa gcggaagagc gcctgaactg gcattttctc cttacgcatc
     3181 tgtgcggcat ttcacaccgg acatggcgca cttttcatac aatccgcact gatgccgaat
     3241 ggttaagcca gtatacactc cgctatcgct acgtgactgg gtcagggctt cgccccgaca
     3301 ccccaaaggt caacggcctg acgcgccctg gcgggcttgt ctgctcccgg catccgctca
     3361 cagacaagct gtgaccgtct ccgggagctg catgtgtcag cggttttcac cgtcatcccc
     3421 gaagcgtgcg aggcaactgc ggtaaagctc atcggcgtgg tcgtgaagcg attcacagat
     3481 gtctgcctgt tcatccgcgt ccagttcgtt gagcttctcc agcagcgtta atgtctggcc
     3541 tctgatgaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt
     3601 gtaagggggg gttctgttca tgggggtaat gataccgatg aaacgcgaga ggatgctcac
     3661 gatacgggtt actgatgatg aacatgcccg gttactggaa cgctgtgagg gtaaacaact
     3721 ggcggtatgg atgcggcggg tctgcctggg tgagccggtt gcccgttccg ggaagctgcc
     3781 gacactggca ccgccgttac tgcgtcagct ggccgccatc gggaataacc ttaaccagac
     3841 agcgaggaag gtgaacagcg ggcagtggtc ttccggtgac cgggttcagg tggtggccgc
     3901 actgatggcc atcggggatg agctgcgccg gctgcgtctg gcggtcaggg aacagggggc
     3961 gcgggatgat agttaagttt catgccaggg gaaaaggtgg tggcagtggt ccggttgatt
     4021 acctgctggg aagggagcgt aaccgggaag gggcaacggt gcttcagggg aatccggaag
     4081 aggtccggga actcatcgat gccacgccat ttgcgaagaa atacacgtca ggtgttctgt
     4141 cgttcgcgga gaaggagctg ccgcctggag ggcgtgaaaa agtgatggcg agctttgagc
     4201 gtgtactgat gcccggtctc gagaaaaatc agtacagcat cctgtgggtg gagcaccagg
     4261 acaagggacg gcttgagctg aattttgtca ttccgaacat ggagctacag accggaaaac
     4321 gcctccagcc gtactacgac cgggcagaca ggccgagaat tgatgcctgg cagacgctgg
     4381 tgaatcacca ttacggtctg catgacccca acgccccgga gaaccgccgg acgctgaccc
     4441 ttcctgataa cctgccggaa acgaaacagg cgcttgctga gggcgtcaca cgcgggatag
     4501 acgcgcttta ccatgccgga gagataaaag gccgtcagga tgtgattcag gcgctcacag
     4561 aggcggggct tgaagtggtc agggtgacgc gaagcagtat cagcattgca gacccgaacg
     4621 gcgggaggaa tatcagactg aaaggagcgt tttatgagca atcttttaca gacgggcgcg
     4681 gagttcgaga aaaagctgaa agagagagcc gaatctacag agaaaatgct gaacgacgag
     4741 ttcaggaagc tcgaagaatc tgtaaacagg gaactgacct caaacgaatc tttaatcagg
     4801 aacgctatca acgaccatac aacagccctg aaagaactgc tggagaggta tcagaaaacg
     4861 accgtcgata cgatggacgc gcactggaaa accgtactga aaatgagcgt gaaacgctgg
     4921 ctgtggctga ttatcgtctc agtactgatg tttgccacga ccgggagtct gctgtggtac
     4981 cagggcatga agataaacgc gaatatgaat attctccgcg agcagaaaga gagtctggag
     5041 aagctgaacg cgaagacatg gggcgtgcgt tatcacgagg acagcaacgg acgttttctg
     5101 gtgttgccga aggggatgaa agccgaaacg aactggacga aggacaacgg caaactgaac
     5161 gcagtgagac tggtgcagga gtgacagagc atgacggagc tggaaagaca tttgctgaac
     5221 gcattagagc aactgcaaca ggactatatg cagcggctga acgaatggga gaacgccttc
     5281 gtggaattgc agaaaatgtt ttcgcttacg caacgggaca acgcgatgct gaacgggcga
     5341 gtcatgcagt tgagtcagca ggtgcagcac ttgagcgagc ggacagaacg cttgagccag
     5401 ttatacagcg agaacaggag ataagggatg aacggctgat gcaggaacgc gaacatgagt
     5461 tatctctgga gcgggagcgt cagccggaaa tccaggagcg gacactggat ggcccttcgc
     5521 tgggatggtg aaaccatgaa aaatgacgtg ttcagtggat taaggcgggc taatgtggcc
     5581 tgtagcatct ggttgcatag gtattcatgc ggttaaaatt tatcaggtgc g
//
