LOCUS       NZ_OP242254             2920 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA033 plasmid pLAO21, complete sequence.
ACCESSION   NZ_OP242254
VERSION     NZ_OP242254.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363337
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600385.1-RS_2024_03_23
            Annotation Date              :: 03/23/2024 03:10:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242254.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA033"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO21"
     gene            join(2730..2920,1..313)
                     /gene="ddp1"
                     /locus_tag="PUP11_RS00005"
     CDS             join(2730..2920,1..313)
                     /gene="ddp1"
                     /locus_tag="PUP11_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 1"
                     /protein_id="WP_094316182.1"
                     /translation="MKQIKFRLTDEEYNVAHVNAISNGVNSVNAFAKRKVLDVIATPSK
                     VIVSNEPSVPVKTCLYPHELELIKRNAKLHGMSVSREIAIRLRKSVLKNEVCLYPEEVQ
                     VLRKLSTTINRVGRNIHFIISGDRFCTVNDPDFRNEIKELTALCETVDKTIEQLVFSVN
                     NRFG"
                     /name="ddp1"
     gene            1078..1638
                     /locus_tag="PUP11_RS00010"
                     /old_locus_tag="KAOIOJKO_00002"
     CDS             1078..1638
                     /locus_tag="PUP11_RS00010"
                     /old_locus_tag="KAOIOJKO_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007848.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_094316184.1"
                     /translation="MSYSRYKRNPFLEGMVVPVKGKQVRLSRLGGDENILVNQSTGEVQ
                     GTHVATFKRVDSEQFVKLFTANIGLTFELSSAGIKAFGVLVWSVQNSALSKDEVDLDSF
                     VLGEFLECQVSASKQPLRLSLATFKRGLNELEKAKIIAKTMRQGRYFINPNFVFNGDRI
                     AFTTVIERKNSIGKQEDTSNEAK"
                     /name="replication/maintenance protein RepL"
     gene            1625..2416
                     /locus_tag="PUP11_RS00015"
                     /old_locus_tag="KAOIOJKO_00003"
     CDS             1625..2416
                     /locus_tag="PUP11_RS00015"
                     /old_locus_tag="KAOIOJKO_00003"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094316183.1"
                     /translation="MRRSNQFPPGSLNIKLKTPLTKYAIMEIMAEYLSMLELVLGVEEF
                     KLYAYLECIVNGEKQAIYHKDKNAIVSEFTLTSMEKIKNSKLDKVAYAEDLIKEGDDEK
                     IKSILNVLNMNTESCAYETFKDKLMLVPSMVVNDLIERKLHLMEIERERKINEDRERLK
                     KEREKEKGVLKIIYNNKLSEYSCSFDYFKAVYFSSVGFLVSEKQKAKYFLAEGLESLLE
                     SYIGKKVVRLSLKGEDRKVSVIEIYNENGEFLFRTKPLTRN"
                     /name="hypothetical protein"
     Typing          complement(549..808)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.571"
                     /coverage="99.0"
                     /evalue="3.1e-97"
ORIGIN
        1 actggagtta ataaagcgta atgctaaatt acacggaatg agtgttagcc gagaaatagc
       61 gatacgctta cgtaaatcag ttttgaaaaa cgaagtttgt ctttatccag aagaagtaca
      121 ggtgctcaga aagctttcaa ctacgattaa tagagttggc cgaaatattc attttataat
      181 tagcggcgat cgattttgca cagttaacga tcctgatttc agaaatgaga taaaggaact
      241 tacagcatta tgcgaaacag tcgacaaaac aatagagcaa ttagttttta gcgttaataa
      301 tcgttttggg taaaaaagta ttatcaagaa ctgactgtag gtcactgggc aaacgagcga
      361 aatgctagca ttttgcggcg tttgccctat cctgcatcgg agttaaacca cgaagatttt
      421 tattctcgtt cttcggaacg accgagccgc cacggttccg gcagcgcctt gagcgctacc
      481 ggaaaccgtc cggggttctc cccggacccc ccatcgctgt aacaccgacg gtctacgacc
      541 acctaccaac cgagtggctg cttgtaccgt gcaaatgggc tttgcaggag gctggcgcgg
      601 ttcctggcga cctgaaagcc cctagttagg ggtatcggcg cgcctgacct cctgaaaccc
      661 tctgtaagcg tttttcgcgc cttccatgcc ctggcgggca tctaaacccc gtttcgtgcg
      721 tctgagagct tctgagagcg ttttaggggc tatctggcga ctcatccccg aaagaacaac
      781 aacgggataa ccgacaacat gcgagaggag gatccgctcg ccgcagccct agcgaccgag
      841 cgtagcgagc gagtgggcga ggaagcggaa gagagccaat ggaaaaatac gcacttacgc
      901 actaccgtta agaactaaga aggagatctc tcgcaacccg catgaaatgg ggatcgtgtt
      961 ttcaggatct gtactacggt agctcaaccc tgttgctacc gaagctcaaa ataatccttg
     1021 tttttgagct accgcgtatc tatgattagc tcctgtgtat catttttatg gtgaaatatg
     1081 agctacagtc gctataaaag aaacccattt ttagaaggga tggttgtacc tgtcaagggg
     1141 aagcaggtta ggctttcgcg tctcggaggc gatgaaaaca ttctggttaa ccaatcaacg
     1201 ggagaggttc aaggaaccca tgtagcaact tttaaacgtg ttgatagcga acagtttgta
     1261 aaacttttta cagcaaatat tggcttaact tttgaactat cttctgcagg aattaaagcc
     1321 tttggtgttt tagtttggtc tgttcagaac tctgcacttt caaaagacga agtggatttg
     1381 gatagctttg ttttgggtga gtttcttgag tgccaagttt ctgctagcaa acagccatta
     1441 cgattaagtc ttgcaacatt taagcgaggt cttaatgaat tagaaaaagc gaaaataatt
     1501 gcaaaaacaa tgcgacaagg aaggtatttc attaacccta actttgtttt taatggtgac
     1561 agaatcgcgt ttacaactgt tatcgagaga aaaaatagca ttggaaaaca agaggatact
     1621 agcaatgagg cgaagtaatc aatttccacc tggttcttta aatataaaat taaaaacgcc
     1681 attaacaaaa tatgccataa tggaaattat ggcagaatat ttgtctatgc tggaactggt
     1741 tttaggtgta gaggagttta agctctatgc ttatttagaa tgcatagtta atggggaaaa
     1801 gcaggctata tatcataaag ataagaatgc gattgtttca gaattcacgt tgacatcaat
     1861 ggaaaaaata aaaaatagta aattggataa ggtagcatat gctgaagatt taataaaaga
     1921 aggcgatgat gaaaaaataa aatcaatctt aaacgtgcta aatatgaata ccgaatcttg
     1981 tgcgtatgag acttttaaag ataaattaat gctagtccct tctatggttg taaacgattt
     2041 aattgaaagg aagctccatt taatggaaat tgagcgagag cgcaaaataa atgaagaccg
     2101 agaaagactt aagaaggaaa gggagaagga aaagggggtg ctaaagatca tctacaataa
     2161 taaattatct gagtattctt gttcttttga ttattttaaa gcggtgtatt tttctagtgt
     2221 tggcttttta gtttcagaaa aacaaaaggc taaatatttt ttagcggaag gactggagtc
     2281 acttctggaa agctatatag gtaaaaaagt agttagatta tctcttaaag gggaagatag
     2341 aaaggtttct gtaattgaaa tatataatga aaatggtgag tttttattta gaacaaaacc
     2401 attgacaaga aattaagagt atctgtggag tataatcatt tttcttttgt aggctttagt
     2461 attgatagct gactcatttc aaaacgaggg aatattgacg ttcaaaaaca ttaaccagta
     2521 cgaaagaaaa attgacttgt tcatccatcg acttattaaa atctattgtt ttgttgcgaa
     2581 tgctcttact cacaatcttt gagcaaaact gcttaaatag gctgcgatca atgcaggata
     2641 gacaatcatg gtcaaatggg taccattttc catgattgtc caaaaactga gtttttgcct
     2701 gtgcctgcgg caggctctta ggaggtacta tgaagcaaat taaattcaga ctgaccgacg
     2761 aggaatacaa cgttgctcat gttaatgcta tttcaaatgg cgttaatagt gtaaatgctt
     2821 ttgcaaagag gaaggtttta gatgttatcg caacaccttc aaaggttatc gtatcgaatg
     2881 aaccatctgt accagtaaaa acatgtttat acccacatga
//
