LOCUS       NZ_OP242250             5153 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA033 plasmid pLAO20, complete sequence.
ACCESSION   NZ_OP242250
VERSION     NZ_OP242250.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363337
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600345.1-RS_2024_03_23
            Annotation Date              :: 03/23/2024 03:13:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 8
            CDSs (total)                 :: 8
            Genes (coding)               :: 8
            CDSs (with protein)          :: 8
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242250.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA033"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO20"
     gene            complement(74..493)
                     /locus_tag="PUP11_RS00005"
                     /old_locus_tag="CDMLIFCF_00001"
     CDS             complement(74..493)
                     /locus_tag="PUP11_RS00005"
                     /old_locus_tag="CDMLIFCF_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(574..1011)
                     /locus_tag="PUP11_RS00010"
                     /old_locus_tag="CDMLIFCF_00002"
     CDS             complement(574..1011)
                     /locus_tag="PUP11_RS00010"
                     /old_locus_tag="CDMLIFCF_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001318234.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     gene            1271..2836
                     /locus_tag="PUP11_RS00015"
                     /old_locus_tag="CDMLIFCF_00003"
     CDS             1271..2836
                     /locus_tag="PUP11_RS00015"
                     /old_locus_tag="CDMLIFCF_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_000447003.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSSI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTINE
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            complement(2833..3174)
                     /locus_tag="PUP11_RS00020"
                     /old_locus_tag="CDMLIFCF_00004"
     CDS             complement(2833..3174)
                     /locus_tag="PUP11_RS00020"
                     /old_locus_tag="CDMLIFCF_00004"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="WP_000058755.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 family microcin immunity protein"
     gene            3222..3359
                     /locus_tag="PUP11_RS00025"
     CDS             3222..3359
                     /locus_tag="PUP11_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001329514.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            3918..4073
                     /locus_tag="PUP11_RS00030"
     CDS             3918..4073
                     /locus_tag="PUP11_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001490772.1"
                     /translation="MSVDFCDARQGGGAYGKTPAALASSGALLFAHMFFPALSPDSVDN
                     RITAFE"
                     /name="hypothetical protein"
     gene            complement(4201..4395)
                     /locus_tag="PUP11_RS00035"
                     /old_locus_tag="CDMLIFCF_00005"
     CDS             complement(4201..4395)
                     /locus_tag="PUP11_RS00035"
                     /old_locus_tag="CDMLIFCF_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041621.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTRFEVENSTER"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4679..5002
                     /gene="mobA"
                     /locus_tag="PUP11_RS00040"
                     /old_locus_tag="CDMLIFCF_00006"
     CDS             4679..5002
                     /gene="mobA"
                     /locus_tag="PUP11_RS00040"
                     /old_locus_tag="CDMLIFCF_00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     Typing          4431..4670
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.585"
                     /coverage="100.0"
                     /evalue="3.54e-121"
     Typing          complement(3643..4172)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.59"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          4680..4999
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="3.11e-62"
ORIGIN
        1 gcggtgtctg cgtcagaatc gcgttcagcg cgtttcagtg gtgcgtacaa ttaagggatt
       61 atggtaaatc cacttactgt ctgccctcgt agccatcgag ataaaccgca gaaatcgtgt
      121 cagccagcag cctggatttt ttcggggtaa ggtctttgct gcggtaatca gtacccgcaa
      181 attgagttgt gccccggagt gagtttaaaa attcctggct gtgaaactgt tgaatgcgtt
      241 gttgcacatc actccgtgat tttcctgtcg ctttatcttt agctcccata tcccagaact
      301 gccgaaaaaa aggcaccatc attcgtgttg tgtcggcttt ttgggtggaa aagttgggat
      361 caaaaccgtc atcagaagca tatacgtaat gccgggtatg ttgttccacg ctgccctttg
      421 gggtacaggc tgtgagagct actgttgctg ttatcatggc gataagacaa agtttgtttc
      481 cggtacgtct catgagtgtc tccctacctt aaagtattta ttctcggtga gttattatcc
      541 gaaggttatc ttatcatcag tttctgagcg cgttcagtga ccgaacagcc tggaccagaa
      601 tccccgtttc ttttcctgct ccagagcctg cctgagctgg gctatctcat tctgtagctg
      661 tttccgttct gcttcctggc gccggcgctc cgtatcctgg gcttgtttat cctcaagcat
      721 cagcgtcagg cattgtttca gctcattcag ttcccggaga atgcgttctg tctgctgatc
      781 atgaggtttt tctgagtgtc cttgagtgtg cctttctggt gtctcattcc gctttaattc
      841 gccgtatgcc cggatcagtt cactggtttc aaactcccgt cgtccgtcct ggctggtacg
      901 gtaactgaca agacctgagc gcatatcacg gtaaaactgt gaacgcgatc ttccagtcat
      961 tttcagtgcg tcccggagtg tgtgccatgc cataaagtta cagtgtccca taggtgtctc
     1021 atctcatggt ttcagtaaaa cataatgagg tctgagaacg gtaatgtttg tgctggtttt
     1081 tgtggcatcg ggtggaaata gcacgtggtg tgggtggctg tttttttgat cgttttcaca
     1141 aaaatgaaaa tccacagggt tgacagggaa aatgcagcgg cgtaatttta aactgtatat
     1201 aaaaccagtg attatatata cagtatattg cttttaacta tttgttttta atgtcaaaga
     1261 ggattttata atggaaacag ctgtagcgta ctataaagat ggtgttcctt atgatgataa
     1321 ggggcaggta atcattactc ttttgaatgg taatccagac gggagtggct ctggcggtgg
     1381 tggtggaact ggaggtagca aaagtgaaag ttctgcagcc attcatgcca cagctaaatg
     1441 gtctactgct caattgaaga aaacgcaggc agaacaggct gcccgagcaa aagctgccgc
     1501 agaagcacag gctaaagcaa aagcaaaccg ggatgcgctg actcaacatc tgaaggatat
     1561 tgtgaatgag gcgcttcgcc ataattccac tcatccggag gttattgacc tggctcatgc
     1621 caataatgca gcgatgcagg cagaagcaga gcggttgcgc cttgcaaaag cagaagaaaa
     1681 agcccgtaaa gaagcggaag ctgcggaaaa ggcttttcag gaagcagaac aacgacgtaa
     1741 agagatagag aaggagcagg ctgaaacaga acgccaattg aaactggctg aggatgaaga
     1801 gaaacgcctg gcagcattga gtgaagaggc ccgggctgtg gaggtggcac aaaaaaatct
     1861 tgctgctgca caatctgagc tggcgaaagt ggatgaagag attaatacgc tcaatacccg
     1921 tttaagctcc agtattcatg cccgtgatgc agaaacgaat acgctgtccg gaaaacgaaa
     1981 tgagttggat caggcatctg ctaaatataa agaactggat gaaagggtta aacttttatc
     2041 tcctagggcg aatgatccgc ttcagagtcg tccttttttt gaggccacca gactacgggc
     2101 gagagctggt gatgagatgg aggaaaaaca aaagcaggta acagcatcag aaacgcgtct
     2161 taaccagatt agctctgaga taaatggaat ccaagaggct atttctcagg ctaataataa
     2221 gcgaagtaca gcagtttcac gtattcatga tgctgaagat aatttgaaaa cagcacagac
     2281 taatctcctg aactcgcaga ttaaggatgc tgtggatgca acagttagct tttatcaaac
     2341 gctatctgaa aaatatggtg aaaaatattc aaaaatggca caggaacttg ctgataaatc
     2401 taaaggtaag aaaatcagca atgtgaatga agctctagct gcttttgaga aatacaagga
     2461 tgtattaaat aagaaattca gcaaagcaga ccgtgatgca attttcaatg cactggaggc
     2521 ggttaagtat gaagactggg ctaagcattt agatcagttt gccaagtact tgaagattac
     2581 gggacatgtt tcttttggat atgatgtggt atctgatatc ctaaaaatta aggatacagg
     2641 tgactggaag ccgctatttc ttacattaga gaagaaagct gtagatgctg gagttagtta
     2701 tgttgttgtt ttacttttta gtgtgcttgc tggaactaca ttaggtatct gggggattgc
     2761 tattgttaca ggcattctat gtgcctttat tgataagaat aaacttaata ctataaatga
     2821 ggtgttgggt atttaattat tctttattga tataaacagt cctagcaatc caaatgggat
     2881 tgctaggacc aaacaaagta gattgtatag cataaatagg tttaattttg ccacgggggc
     2941 attatttagg ttttttcttt ttctgaaaaa atctttcttt atgaacttaa aaaatatgtg
     3001 ttcaatagca tattttgaat atgggcatag aatagtgctt atcactattg cgtatagcat
     3061 cttatctgac gctaggaaat aatacccttc gttgtttttt gttataaggt atatatatat
     3121 aagtgcgcag tataggccaa acaaaatatt ttttatgtag tatcttaaac tcataaatta
     3181 aacctcgtta tatattcttt tcattttata aggatcgagt tatgaggaaa agattttttg
     3241 tgggaatatt cgctataaac ctccttgttg gatgtcaggc taactatata cgtgatgttc
     3301 agggagggac tgtggctcca tcatcttctt ctaaattgac ggggatctct gttcagtaga
     3361 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcctgtaaac
     3421 gaaaaaaccg cctggggagg cggtttgatc gaaggttaag tcagttgggg aactgcttaa
     3481 ccgaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcca gtgtagccgt
     3541 agtcgggcca ctacttcaag aactctgttg caccatttgc gccatcatcg ctctgctaac
     3601 caagttacca acggctgctg ccagtggcgc taagtcgtgt cttaccgggt tggactcaag
     3661 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3721 cagcttggag cgaacgacct acaccgagcc gagataccaa cagcgtgagc tatgagaaag
     3781 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3841 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3901 gtttcgccac ctctggcttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3961 atggaaaaac gcctgcggcg ctggcttctt ccggtgcttt gctttttgct cacatgttct
     4021 ttccggcttt atcccctgat tctgtggata accgtattac cgcttttgag tgagctgaca
     4081 ccgctcgccg cagtcgaacg tccgagcgta gcgagtcagt gagcgaggaa gcggaagagc
     4141 gccttatgtg acattttctc cttacgctct gttgtgccgt tcggcatcct gtcctgagcg
     4201 ttatctctct gtgctatttt ctacttcaaa gcgtgtctgg atgctgttct ggagttcttc
     4261 tgcgagttcg tgcagcttct cacacatggt agcctgttcg tcagcatcga gtgcgtccag
     4321 cttttcgagc agcgtcaggc tttggctttt tatgaatcct gccatgttga gtacggcttg
     4381 ctgctgcttg ttcatctttt cgttttctcc gttctgtctg tcatctgcgt tgtgtgatta
     4441 tatcgcgtac cacttttcga ctgttttacc gccggtattc tgccgtctga cgctttgacg
     4501 ggtatttctg cctgacaata ctgtcactgc caaaaaactg ccgtgtcttt gtcggtaatt
     4561 cgaacttgct gacaggacag gatgtgtaat tgttataccg cgcatgcatg cacgctatta
     4621 caattaccct ggtcagggct ttgccccgac acccatgtca gatacggagc catgttttat
     4681 gacaaaacga agtggaagta atacgcgcag gcgggctatc agtcgccctg ttcgtctgac
     4741 ggcagaagaa gaccaggaaa tcagaaaaag ggctgctgaa tgcggcaaga ccgtttccgg
     4801 ttttttacgg gcggcagctc tcggtaagaa agttaactca ctgactgatg atcgggtact
     4861 gaaagaagtt atgagactgg gggcgttaca gaaaaaactc tttatcgacg gcaagcgtgt
     4921 cggggacagg gaatatgcgg aggtgctgat cgctattacg gagtatcacc gtgccctgtt
     4981 atccaggctt atggcagatt agcttcccgg agagaaaact gtcgaaaact gacggtatga
     5041 acaccgtaag ctcccaaagt gatcaccatt cgctttcatg catagctatg cagagagctg
     5101 aaaacgatcc tgacgcatcc ttcctgtttt tccggggtaa aacatctctt ttt
//
