LOCUS       NZ_OP242246             1552 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA030 plasmid pLAO16, complete sequence.
ACCESSION   NZ_OP242246
VERSION     NZ_OP242246.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363336
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600305.1-RS_2024_03_23
            Annotation Date              :: 03/23/2024 03:12:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1
            CDSs (total)                 :: 1
            Genes (coding)               :: 1
            CDSs (with protein)          :: 1
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242246.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA030"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO16"
     gene            join(1537..1552,1..503)
                     /locus_tag="PUP00_RS00005"
     CDS             join(1537..1552,1..503)
                     /locus_tag="PUP00_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_252382951.1"
                     /translation="MQTRNKCLQKQVFSGFLGDCAAGKFLKNTEGSCKLNELVSVAANN
                     RLHRNIEEVENLNEPEEARLMAGFFRSTRLQRNKQVQKRNLSAIAHQIYKKRTFTRPNS
                     QKRTRNVFTRLNNYSRTRSADGFDGSTARTGQNGKLSHQKTVNGTRNFFAGREALAGCR
                     GAALTRWQL"
                     /name="hypothetical protein"
     Typing          465..552
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="96.0"
                     /evalue="2.61e-33"
     Typing          complement(907..987)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.683"
                     /coverage="96.0"
                     /evalue="2.04e-24"
ORIGIN
        1 agtgcctgca aaaacaggtt ttttcgggat tcctgggaga ctgtgcggcg ggaaaattct
       61 tgaagaatac agaaggttca tgcaaactga atgaactggt ttctgtcgct gcgaacaaca
      121 gactccacag gaacattgag gaggtcgaaa acctcaatga acccgaagaa gctcgcctaa
      181 tggcgggctt ttttcgttct actcgccttc aacgaaacaa gcaagtccaa aaacgcaatt
      241 tgagcgcgat agcacatcaa atctacaaaa aacgcacttt cacccgacca aacagccaaa
      301 aacgcaccag aaacgttttt acgcgcctga ataactattc acgcacacga tcggcggacg
      361 gtttcgacgg ttcgacggcg cgaacgggtc aaaacggcaa actttctcat cagaaaacgg
      421 tgaacgggac gcggaatttt ttcgcgggcc gtgaggcgtt ggcggggtgt cggggcgcag
      481 ccctgaccag atggcaattg tgatagcgtc gcgtgtgacg gtattacaat tgcacatcct
      541 gtcccgattt tttcgccaga acgctggcag agcatcggca gttctgcgcc gttaacccct
      601 ccacaggagc gcacagtttc ccgacgaagg agagaaacta ctaagtgcgc atccgcactt
      661 aacgcgcgga tgatttttgg caccgcagcc accacagcta cgcaaaaatt tcggcgaccg
      721 gacagcaaat cattttgccg ttttcgtgac gcagccgttc cgcgcaatcg acaaactggc
      781 agtacagaaa actcacgaag tgcacgaagt gtgcacgaag tgcgcactta tacaaacttc
      841 acttcgtttc gtttgtcgtg cggtctgcga cgcgcaaaaa aaccgagaaa cggcgggaat
      901 tttctggaca ggatgtgtaa ttgtaatacc gtcacacgcg acgctatcac aattaccacc
      961 tggtcagggc atccgccccg acaccccgca tcagtgcgta cgtgctcaat gtggtttccg
     1021 gttcacttcc gcttcctcgc ccactcgctc actacgttcg gtcgctgggg ctgcggcgag
     1081 cggagtgatc tctctctcga tcctgtaggg atcttttttt tttttgcggt agagcagtct
     1141 gtgattgcgg tgcggcaggg ggctgtgcca tatgctggtt ggtgagcgtt ctgaggaacc
     1201 ggcaaactcc ccccccaccc cgcaaagggc gtaaaagggg gtaagagttt tgcccgagcg
     1261 atatgctggc cggagccgcg cccatcgtca ccggaacgga cctatggtag aacgtccgtt
     1321 tgcatgttcc cgcgtctacg ccgcgacatt tcgcccaaca tgctggcact ggtcccagaa
     1381 ccgcgcccaa gggcaaaaac ccaatataag cgcattacat tcaaccactt gtgccactcc
     1441 ccaggcgtcc gctcaaaaat gaagtatctt ccccggtagc ggaattctca cgcgtgcggt
     1501 cgccgtcgtc actgaccccc caaccgcaaa cggcctttgc aaacacgcaa ca
//
