LOCUS       NZ_OP242244             4062 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA294 plasmid pLAO104, complete sequence.
ACCESSION   NZ_OP242244
VERSION     NZ_OP242244.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363377
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600285.1-RS_2024_03_23
            Annotation Date              :: 03/23/2024 03:13:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242244.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA294"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO104"
     gene            complement(175..2322)
                     /gene="mobQ"
                     /locus_tag="PUP10_RS00005"
                     /old_locus_tag="GHOBLGOB_00001"
     CDS             complement(175..2322)
                     /gene="mobQ"
                     /locus_tag="PUP10_RS00005"
                     /old_locus_tag="GHOBLGOB_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_070749924.1"
                     /translation="MAIYHLSMKIISRSNGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQKEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NHLINRLNLEIQISHTELRNSGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKLEKQDIPASQTAFEKQWQQWGESGLAALRSQL
                     LKREEELRRKELEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQYPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWDEW
                     RREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            2501..2755
                     /gene="mobC"
                     /locus_tag="PUP10_RS00010"
                     /old_locus_tag="GHOBLGOB_00002"
     CDS             2501..2755
                     /gene="mobC"
                     /locus_tag="PUP10_RS00010"
                     /old_locus_tag="GHOBLGOB_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091744.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            3288..3989
                     /locus_tag="PUP10_RS00015"
                     /old_locus_tag="GHOBLGOB_00003"
     CDS             3288..3989
                     /locus_tag="PUP10_RS00015"
                     /old_locus_tag="GHOBLGOB_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281396.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVEITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(1965..2323)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.5"
                     /coverage="100.0"
                     /evalue="8.52e-174"
     Typing          2324..2500
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          3289..3988
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(179..2409)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.059"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgaaaaatt gcgtcaaaaa cgctctgatg cagcgaaaaa agaaccccca atttttgggg
       61 gcggaaaaac aaaactatgc ttgcgttatg tgagtgtgct tcttgcattc atccctgtcg
      121 atgaacatcg acgaacataa cacgatgtta aacaaaacaa aatattgttc acattcactg
      181 tgaccaaccg cgatcatgtc tcctctcggt ggcgtgtgtt cgtgcatctt cctcgcgccg
      241 ccattcgtcc caggtacgat taaaacccag cgagcggaac agatgatccg gctgtttttc
      301 tggtgtgttg acggcagcaa aaagcggtgc ctcacgggtt ttcagatgaa actccataag
      361 ttccggcgca ggtgatatgt ggctgtctgc atcatgattt acggcaacac cataccctgc
      421 ataccgtaac cccttatcgg tcagtgcact gatgaccagc tggctgttat cggctgaggg
      481 aacggcaaac gcgggataca gcgtaccatt aagccggaca acaccgccca gagagtcatc
      541 cacgcccatg accagcaaaa gctgaagctg gcgggtttcc cggtcgacag aactcatgac
      601 cggcatcagc gtcggtggca gacgtccctg cgtaaaatcc accttccttg ctgccagtga
      661 tggcatggct ggctggcggt cttctccaag acagaaagac tccggccatg tcttcggttg
      721 gcggagattg cgggtatgtt gcggccatgt ctggcaaacc tgcgtcagaa gttgctcaaa
      781 ggtcgcagag gaataaacac gttgcagtgg tggatattga ccgatttcgc ttcggtcaca
      841 gatataacca ctcctgtcac gcattaaatc aacactctca aagatcctgg tgcggtattt
      901 ctccagctcg ttcccccaga gcgtatattc tccgttccgg ttgtgaagca gaaggtaaca
      961 ggtcatttcc ccgttaccag gcatgggagc ctcgccatac cccgtaacga ctgctctcag
     1021 cacccctgaa tccataacgg cattgtcatt tttcctgagc tgctccgcgc gactacgttc
     1081 tgtatcctcc agctccttcc ggcgcaattc ctcttcacgt ttaagcaact gggatctgag
     1141 agctgcaagg ccggattccc cccactgctg ccactgtttt tcaaatgccg tctgactcgc
     1201 gggaatgtcc tgtttctcca gcttccggta atgctgtcgc aatttttcca tttgctggct
     1261 cagagactgg atttttccct gctcactacg gtattccggt gcagcccatt gcatcagggg
     1321 aatacgggaa aaaccggacg aaaacggcga tgattttttc aggctggcag catggctcat
     1381 cgcttccttt cgttcggtgt tcagctcttc ccagcgtctg cgcgtcgcct gcatatctgc
     1441 catcatcgac atggcgttac tggtgagttt ccatgccgcg ttactgtcct tcggcaacgc
     1501 ttcgatgatg tcacgtagtg tgtacgaagt tgcgttgtca ggaagcccga tattcagaag
     1561 gtcagccaca cggcgggcag gctctgtcag cccgctgttc ctgagttcgg tgtgggaaat
     1621 ctgtatttcc aggttaagcc tgttaatcag atgattctgt tcgccgcgtt ctgtttcgat
     1681 accgcgtgtt tccatcgcac aggcagactt tccgaggtga atacccggcg ttttctcaag
     1741 cccctgtcgc tcgtatgaac gatggtctat ctctttctga taaccggcgt tcgccagtgc
     1801 cctgtttgca tggtcagccc atgatgcacg ccacgtctcg gcatgtgtcc ggtcgttcca
     1861 gtccctgacc tttccgccga acccagcagg cccgacagcc ctcgtggtca gcatgatgtg
     1921 cgcatgggga tttgttttat ccatgtgatg gaacgccaca tcagcaatca tgccctgact
     1981 gacaaagttt tctctgacaa aggcgagaac ggtttcccgt gcggcatcct gcggcagttc
     2041 gtgggggatg gcgagttcaa tttcccgcgc aagttgtgag ttcttccggc gttcggcctt
     2101 ctcaacggca ttccagagtt cggcacggtt cgtacaccag gcaggggctg tcgcgggggt
     2161 cagaatcaca gcctcagcca caccgctttt gcgggtgtag tcgtgagtca gtccggtacg
     2221 ctcatcaagc attagtgaac cagaacgata ggcagcagaa gcaacggcgc tgtagccgtt
     2281 gcttctggag atgattttca tactgagatg ataaatcgcc atttctgccg taatgccttt
     2341 ttgcagtttt ctttcagccc cgcagggcgc acacaatcga aacggagtgg agattgtata
     2401 agtgcgcact tcgtgatttg ccgtgcatta tgccagctca gtttttaaac taccagtgac
     2461 gcgctaaaat gcgatttttc atcgcgcatg gagttacatc atgagcagaa cgctcgaaca
     2521 gaagattgct gatgctgagg cgagattaca gcgcctgaag gcgaagagcc ggagcctgga
     2581 cacagcgcag aaggtcattg tgggtgcagc attgctggca aaagtcagaa agccggagga
     2641 agtgcagttg cgagcttggt tactccagtt cctgaaggca gaggtgacac ggcaggctga
     2701 tgtgacgcga atactgccgc tgattaacga gctggaggcg ctgccagagc agtgattgta
     2761 tcgtccgctc gccgcagccc agcgaccgag cgttagcgag cgagtgggcg aggaagcgga
     2821 agtgaatcag tcgccatgtt gagcacatac gcacggtttt tcgacaggtg atatgcgctg
     2881 aacccgcatg aatactggat ttccacaggg ttggaggggg tgtattcgga atcttcaatc
     2941 ctgatttttt tcaacacatt gtttttattg gatttttatt ttcagcaccc caagttatcc
     3001 acaggctcgc cgttaagttt ttaagatctt atttttagat cttttttttt aaaacctttt
     3061 taggtcttgt aaccacatga ttttaaaggt gttctggcga gataggtgga gcttttgggg
     3121 gtgataggtg gagcttttgg gggtgatagg tggagctttt gggggtgata ggtggagctt
     3181 ttgggggtgt aatccacaga aaaaaggggt tgcaaaaaaa aaacttccac caatatgatg
     3241 ggtggcgtac agtaaaaaaa cgccaccaat agcaacgagc aagcgaaatg tcagatctga
     3301 tagcttacaa atcaaacgcc cttatcgagg caagctacaa gctcactttg caggaacagc
     3361 gcttcctgct ggtctgtatc agccgcctga aatccggaga agattcccct tcgccggatg
     3421 aacaaaaaac catgaccatc acggctgcag agtttcatga tgcctttcct gacatgggac
     3481 ggcagcacgc tgaagctagg ctcaaggaag cgatagatcg cctctgggac aggtcagtaa
     3541 tcatcaagaa cgaggaaaaa cgagaagagt tccgctgggt gcagtaccgg gcgcagtatt
     3601 tcaaaggtga ggcgaaagta gagatcacct tctccgatgc catcatgccg tatctgacac
     3661 agctcaaggg gcagtttacg cgggtagtcg tgaagaacgt ctccagcctc tccagcacct
     3721 acagcatccg catttacgag ctgctccagc agttccgcag caccggtgac agaacgatag
     3781 ctatcgatga tttccgaacc atgcttgccg ttgaagataa atatcaacaa ttcaaagact
     3841 tgaataagat gataatcaag ccagccatcg ccgaactgaa cgaaaaaagc gatctggttg
     3901 taacggttga gacgatcaaa aaggggcgca cggttgtagc cctgcatttt cgcttcaagg
     3961 aagacaaaca gatcaagatg acgatctaga atcgatttta aggcacctaa aacactccct
     4021 gttgcgtttt ttttgtgtta gtagtgcgaa ggtactattg aa
//
