LOCUS       NZ_OP242241             5330 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA249 plasmid pLAO101, complete sequence.
ACCESSION   NZ_OP242241
VERSION     NZ_OP242241.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363372
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028600255.1-RS_2024_03_23
            Annotation Date              :: 03/23/2024 03:09:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 6
            CDSs (total)                 :: 6
            Genes (coding)               :: 6
            CDSs (with protein)          :: 6
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242241.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA249"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO101"
     gene            320..463
                     /locus_tag="PUP08_RS00005"
     CDS             320..463
                     /locus_tag="PUP08_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272955169.1"
                     /translation="MGIAKSVAKWTNKNFTESSFDDFIKKPHSADIQSLRGKKVKEEVG
                     LH"
                     /name="hypothetical protein"
     gene            534..2213
                     /locus_tag="PUP08_RS00010"
                     /old_locus_tag="FLICCLCG_00001"
     CDS             534..2213
                     /locus_tag="PUP08_RS00010"
                     /old_locus_tag="FLICCLCG_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598747.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_053888063.1"
                     /translation="MIKVQLPTREHHHHELNLNYGDVVFFIGQNGSGKSSLLNNLFQKI
                     GSQSSVYVLAHRKNTFSSDIIDFSNSEYKNAQGWIFNEIKGSQCRYRVNYDSQMVAIPI
                     VKLKNKEIARALSFVDSFKSNNNQPAELIKSEVDVLNMVFADAKLSIEFFLDDEANVMA
                     RNIKGEHPDVYPISMLSDGEKSALIIASEIICADENTTIFLDEPERHMHRSIVSPLLSS
                     LMKYRRDCVFFVATHELSLPAFFDNVKVVNVKNCYFSEQREPMCWDFNVVEDYDELFNG
                     VDEQTKIDILGARDKVLFVEGVTSSLDRDLYSAIFPMVSVVSKQKCDLVELAVKGLRLN
                     DEIHRVSAFGIIDNDNKVQAKVDNLKKDFIFSLDVHSIESIYYHPRMIKFVIDFVKEAN
                     GIDNVDTLFMEIHDFAIDAINEKRDHLCCRAVEKTIRADLMSSIPKQQDIKNRIKFNKE
                     IDIECYVNKEIAAFDAMIAERNLEALVSRYPIRETNLLAHVAKKCGFADRSRYELNVIR
                     TIKSNADARAFVLSLLGGLDEAILGCSDTENSPADTNTVFAM"
                     /name="AAA family ATPase"
     gene            complement(2450..2644)
                     /locus_tag="PUP08_RS00015"
     CDS             complement(2450..2644)
                     /locus_tag="PUP08_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_053888061.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVHFEAENETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3396..3539)
                     /locus_tag="PUP08_RS00020"
                     /old_locus_tag="FLICCLCG_00004"
     CDS             complement(3396..3539)
                     /locus_tag="PUP08_RS00020"
                     /old_locus_tag="FLICCLCG_00004"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272955168.1"
                     /translation="MVPERKQAEGAQEKSRDDKKNPYGLFFVLSSLVHKDITPENMACE
                     SP"
                     /name="hypothetical protein"
     gene            3540..4037
                     /locus_tag="PUP08_RS00025"
                     /old_locus_tag="FLICCLCG_00005"
     CDS             3540..4037
                     /locus_tag="PUP08_RS00025"
                     /old_locus_tag="FLICCLCG_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011899450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_053888062.1"
                     /translation="MRTLLNKDRFVPAPNNYGVAGSPNSAIAHPDGLDCYGRVPEINSK
                     GDVIPAGDIFLRVGRHIGPNRGFGVRHIWAEHEQELVKLGYATVNDVARFVRDIIRRGV
                     PIYCEFNSTSGKHRPAVLKSSIGIVILEPREAPETESGWIYVVVTAYTRRTAHGTLVGH
                     IE"
                     /name="hypothetical protein"
     gene            complement(4631..4714)
                     /locus_tag="PUP08_RS00030"
     CDS             complement(4631..4714)
                     /locus_tag="PUP08_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058652899.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPDRHD"
                     /name="hypothetical protein"
     Typing          2884..3140
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.208"
                     /coverage="89.0"
                     /evalue="3.43e-107"
     Typing          complement(4556..5119)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.965"
                     /coverage="89.0"
                     /evalue="7.01e-162"
ORIGIN
        1 gggcgccgat gcggggtatt cagggctaat ttgcaagaat ccgaaccacc tgcactggca
       61 gatcaccgtt tggcagcctg agctctaggc cctcgactgg ttagccgact acctcgacct
      121 tggcgcagct aatgaccgcg aaatcctacc ggactacggt ttaggccgta actgcaccct
      181 cttcgataaa acccgcaagt gggcttaccg tgctatccgc caaggctggc cgcagtatga
      241 ccaatggcta caagcctgct ttgaacgcgc taaagcctac aacctgcagt tctccgcacc
      301 tttagacgaa aacgaagtca tgggtattgc caaaagtgta gctaagtgga ctaataaaaa
      361 ttttacagag tcatcatttg atgattttat aaaaaagccc cattcggcag acatccaatc
      421 tctacgtggg aaaaaagtaa aggaggaggt aggacttcat taaactctcc atggattaat
      481 ttaggaatta gtcgttcaac gtattttaga ttaaaagcta aagagaaagg aaaatgataa
      541 aagttcagtt gcctactaga gaacatcatc atcatgagct taatcttaat tatggcgatg
      601 tcgttttttt tattggtcag aatggtagtg gtaaatccag cctattaaat aatctttttc
      661 aaaaaatagg atctcaatca tcagtatatg ttttagcgca tagaaaaaat acattcagca
      721 gcgacattat tgatttcagt aatagtgagt ataaaaatgc gcagggatgg atttttaatg
      781 aaattaaagg cagtcaatgt agatacagag ttaattatga ctcacagatg gttgccatac
      841 ctatagtaaa attaaaaaac aaagagatag ccagggcttt aagtttcgta gattcattta
      901 aatcaaataa caatcagccg gcagagttaa taaagtcaga ggttgatgtt ctaaacatgg
      961 tttttgctga tgctaaatta tctattgagt tctttctaga tgatgaagcc aacgttatgg
     1021 cacggaatat taaaggtgaa catcctgatg tttatcctat atcaatgctt tctgacggtg
     1081 aaaaaagtgc actaattatt gccagtgaga ttatttgcgc tgatgaaaac acaactattt
     1141 tcttagatga gcctgagcga catatgcaca gaagcattgt gtctccgctg ctttcatctt
     1201 taatgaaata tcgtagagat tgtgtttttt ttgttgccac ccacgagtta agcttgcctg
     1261 cgttttttga taacgtgaaa gtggttaacg ttaaaaactg ctatttttcc gaacaaagag
     1321 aaccgatgtg ctgggatttc aatgttgttg aggattatga tgaattattc aatggtgttg
     1381 atgaacaaac taaaatagat atacttggcg ctagggataa ggttctattt gttgaaggtg
     1441 ttacgtctag ccttgaccgt gatttatatt cggccatatt tcctatggtt tcagttgtat
     1501 ctaaacaaaa atgtgatttg gttgaattgg cggtaaaagg cttgagacta aatgacgaaa
     1561 ttcatcgagt atcggcgttt ggtatcattg ataatgataa caaggtgcag gcgaaggtcg
     1621 ataacttaaa gaaagacttc atttttagcc tcgacgttca ttctattgag tcgatttatt
     1681 atcatcctag aatgattaag tttgtcattg actttgtaaa agaggctaat gggatagata
     1741 atgttgatac cttatttatg gaaattcatg acttcgctat tgatgccata aatgaaaagc
     1801 gcgatcactt atgctgccgt gcagttgaaa aaacaataag ggcagactta atgtcgtcca
     1861 taccaaaaca gcaagatatt aaaaaccgca ttaagtttaa taaagaaatt gatattgaat
     1921 gttatgttaa taaagaaatt gctgcatttg atgcaatgat cgcggaacga aatttggagg
     1981 cgttagtctc acgctaccct atccgagaaa caaatttgtt ggcacatgta gctaaaaaat
     2041 gtggttttgc cgatcgctct agatatgagt taaacgtgat tcgtacgatc aagagtaatg
     2101 ctgacgctag agcatttgtt ttatccctcc tcggagggtt agatgaagct attttgggat
     2161 gttcagatac ggaaaatagc ccagctgata ccaatacagt ttttgctatg tgagactaaa
     2221 caacctatat cagataacag ccctgctttt gcggggcttt ttgtttgtgc gtgacttgac
     2281 cgcgatgtac gatagttgct gtattaagtc agtatacact ccgctatcgc tacgtgactg
     2341 ggtcagggct gcgccccgac acccgttaaa acccgctgac gcgccctgac gggcttgtct
     2401 gctgacgaaa atgctcacgg gcaagccgtg accgacttcg tcagcttcgt tatgttcctg
     2461 tttcattttc ggcctcaaag tgaacctgga tgctgttctg gagttcttct gcgagttcgt
     2521 gcagtcgttc acacatggcc gcctgttcgt cggcatccag agcatccagt ttttcaagca
     2581 gtgttaggct ctggcttttg ataaatcgcg ccatattcag cgcagtttgt tgctgtttgt
     2641 tcaccttacg cgttctctgt cggtgtcggt tggtccctga ctaggtggta attgtaagag
     2701 gttgcatgcg acggtattac aattgcatat cctgtcctgt ttcttcaggc agtattatgg
     2761 ccggataaat aatacctgtg cacaacagcc gcggttgcat aaaatctcgc tctgcggcaa
     2821 gtgtttttta atcaaacatt ccgttgatac tccggaaaaa cacgagtctg ttctcatgcc
     2881 tgaaatgccc acaccccacg caaaaacaag tttttgctga tttttctttt ataaatagag
     2941 agttatgaca aattagttgc tcttactctc tttgtgatat ttaaaaaagc ggtgtcggcg
     3001 cggctacaac accgcgccga caccgctttg taggggtggt actgactatt ttcataaaaa
     3061 acatcatttt atattagggg tgctgctagc ggcgcggtgt gtttttttac aggatgcccc
     3121 tggtggcgct gctaggggtg tctgttcaga tatgtagcaa tgagtcaggg attcacgaac
     3181 tgaaaatgtc cgaaaatgtg tctcatgggc accgtatgcg cccaaaccgt tcactattca
     3241 ctttcatgaa tagctatgca gcgagctgaa aacgatcctg acgcattttt tcggtttgcc
     3301 ttggggaaaa catctctttt tgcggtattt gcgtcagaat cgcgttcagc gcgtttctgc
     3361 ggtgcgggca acggatgtta tggtaaatct agctgctagg gcgattcaca cgccatattt
     3421 tcaggtgtga tatccttatg caccaaggat gaaaggacaa aaaataatcc ataaggattc
     3481 tttttatcgt ctctgctttt ttcttgggct ccttccgctt gtttcctctc tggtaccata
     3541 tgcggactct actcaataag gatcgttttg tgcctgctcc aaacaattac ggtgtggctg
     3601 gttcacctaa cagcgctata gctcatcctg atgggcttga ttgctatggc cgtgtccctg
     3661 aaattaattc taaaggtgat gttattcctg ctggcgacat cttcttgcgt gttggtaggc
     3721 atattggccc taaccgggga tttggtgttc gccatatctg ggctgagcac gagcaggaac
     3781 tggttaagct gggatatgcg accgttaatg atgtggctcg tttcgttcgg gatattattc
     3841 gtcgtggcgt accaatatat tgcgagttca acagtacaag cggtaaacat cggccagcag
     3901 tgctaaagtc gtcgataggc atcgtaattc ttgagcccag agaagcgcct gaaactgagt
     3961 ctggttggat ctatgtcgtt gtgactgcgt acacacgaag gacggcgcac ggtactttgg
     4021 ttggtcatat tgaataaaaa aatagggcgc taaaaagcgc cctttatgaa ttcgttggta
     4081 gtcgcagcat ttccttagca catagggttc agaaaccgac gcttgtcgta actacccggc
     4141 agatgaccag ccatctgact gcccggcaca gaaggcctat tactggttga tgccccctgc
     4201 gaatgctctt gcagtacaat aatcataaac atcgtgatgt agatttgcaa ccaacatttt
     4261 ggttgcaacc tatatactaa tttttctcga taacgccgga atccattgct gtgtctggct
     4321 ttcttgaagc tcctgactat agatacccta tatcctaaca gttattctgc tgtggatatc
     4381 agaaatgaac acttgagatc atcagtggct cgtcgtagtc tcttgctcag aaacgaaaaa
     4441 accaccctgg caggtggttt tttcgaaggt tagttaatcc tggcagattc tctaaccgtg
     4501 gtaacagtct tgtgcgagac atgtcaccaa atactgtcct ttcagtgtag cctcagttag
     4561 gccgccactt caagaactct cgttacatct ctcgcacatc ctgcttacca atggccgttg
     4621 ccaatggcga ttagtcgtgt ctatccgggt tggactcaag acgatagtta ccggacaagg
     4681 cgcagcggtc ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct
     4741 acccggaact gagtgtcagg cgtggaatga gataaacgcg gccataacag cggaatgaca
     4801 ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg agccaccagg gggaaacgcc
     4861 tggtatcttt aagtcctgtc gggtttcgcc accactgatt tgagcgtcag attctgtgat
     4921 gcttgtcagg ggggcggagc ctatggaaaa acgccagcgg cgcggccttt tgccctggct
     4981 ttgccttttt gctcacatgt tctttccggc cttatcccct gattctgtgg ataaccgtat
     5041 taccgtctct gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtt
     5101 agtgagcgag gaagcggaag agcgtaggca tttgcatttt ctccttacgc atctgtgcgg
     5161 catttcacac cgtccatggc gcacttttca tacaatccgc actgctaccg catcgttaag
     5221 tcagtactca cagcgtcccc tctgcgaggt gccttctgtg actggttcag ggggctcgcc
     5281 gcccccctca aatatgctgc cgccattgag aatgcgctgc gtaaaaaatt
//
