LOCUS       NZ_OP242237             7849 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA124 plasmid pLAO60, complete sequence.
ACCESSION   NZ_OP242237
VERSION     NZ_OP242237.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363350
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7849)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7849)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Sequencing Technology             :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028600215.1-RS_2024_03_23
            Annotation Date                   :: 03/23/2024 03:12:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 9
            CDSs (with protein)               :: 9
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242237.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA124"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO60"
     gene            1..840
                     /locus_tag="PUP03_RS00005"
                     /old_locus_tag="JALKJMPE_00001"
     CDS             1..840
                     /locus_tag="PUP03_RS00005"
                     /old_locus_tag="JALKJMPE_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_112928755.1"
                     /translation="MATHKPIDILASFTELPPPIDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLDVGELRTGPVIYLPAEDPPTAIHHRLHALGEHLTDEQRQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSGMTTGEAEE
                     WGVDDTQRPYFVRFGVSKANYGAPFKDRWFRRHDGGVLKPAVLEKQHKSKGVPRGKA"
                     /name="helicase RepA family protein"
     gene            827..1708
                     /gene="repC"
                     /locus_tag="PUP03_RS00010"
                     /old_locus_tag="JALKJMPE_00002"
     CDS             827..1708
                     /gene="repC"
                     /locus_tag="PUP03_RS00010"
                     /old_locus_tag="JALKJMPE_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_272954245.1"
                     /translation="MAKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRGKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPSGLVLEPEPETPGGQQLRLFLEPKWEAV
                     TADAMVVKGSYRALAREIGYANIEDSKPIRDCIERLWKVSIIAQNGRKRQGFRLLAEYA
                     SDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGKTG
                     KAAIDTLCGYVWPSEASGAAMRKRRQRVREALPELIALGWTVNEYAAGKYDIARPKAAG
                     WLTPAITVTPPR"
                     /name="repC"
     gene            complement(2295..2555)
                     /locus_tag="PUP03_RS00015"
                     /old_locus_tag="JALKJMPE_00004"
     CDS             complement(2295..2555)
                     /locus_tag="PUP03_RS00015"
                     /old_locus_tag="JALKJMPE_00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_112928751.1"
                     /translation="MAKAAERLAKLEEQRARINVEIQRVRAREQQQKRKEDTRRKVLVG
                     AWILGKVESGEWPEQRLLDGLDSYLERDHDRALFGLPPKQE"
                     /name="mobilization protein"
     gene            2738..>3379
                     /locus_tag="PUP03_RS00020"
                     /pseudo=""
     CDS             2738..>3379
                     /locus_tag="PUP03_RS00020"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            3486..3896
                     /locus_tag="PUP03_RS00025"
     CDS             3486..3896
                     /locus_tag="PUP03_RS00025"
                     /function="putative role in conjugative DNA replication"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_011117300.1"
                     /translation="MSAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSAARGWTWKLWAGVLIASV
                     TPILALLIASWLWLEPQIIEQQGGIWLVFKLK"
                     /name="IncQ-type mobilization protein MobB"
     gene            <3995..4849
                     /locus_tag="PUP03_RS00030"
                     /pseudo=""
     CDS             <3995..4849
                     /locus_tag="PUP03_RS00030"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /name="DNA-primase RepB domain-containing protein"
     gene            4995..5558
                     /locus_tag="PUP03_RS00035"
                     /old_locus_tag="JALKJMPE_00006"
     CDS             4995..5558
                     /locus_tag="PUP03_RS00035"
                     /old_locus_tag="JALKJMPE_00006"
                     /EC_number="2.3.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491968.1"
                     /GO_function="GO:0047663 - aminoglycoside
                     6'-N-acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0030647 - aminoglycoside antibiotic
                     metabolic process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AacA4 family aminoglycoside
                     N(6')-acetyltransferase"
                     /protein_id="WP_112928758.1"
                     /translation="MGRKAASTPPITLRLMTERDLPMLHDWLNRPHIVEWWGGDEERPT
                     LDEVLEHYLPRAMAEESVTPYIAMLGEEPIGYAQSYVALGSGDGWWEDETDPGVRGIDQ
                     SLADPTQLNKGLGTRLVRALVELLFSDPTVTKIQTDPTPNNHRAIRCYEKAGFVREKII
                     TTPDGPAVYMVQTRQAFERKRGVA"
                     /name="AacA4 family aminoglycoside N(6')-acetyltransferase"
     gene            5642..6541
                     /locus_tag="PUP03_RS00040"
                     /old_locus_tag="JALKJMPE_00007"
     CDS             5642..6541
                     /locus_tag="PUP03_RS00040"
                     /old_locus_tag="JALKJMPE_00007"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase VEB-17"
                     /protein_id="WP_063865157.1"
                     /translation="MKIVKRILLVLLSLFFTIAYSNAQTDNLTLKIENVLKAKNARIGV
                     AIFNSNEKDTLKINNDFHYPMQSVMKFPIALAVLSEIDKGNLSFEQKIEITPQDLLPKM
                     WSPIKEEFPNGTTLTIEQILNYTVSESDNIGCDILLKLIGGTDSVQKFLNANHFTDISI
                     KANEEQMHKDWNTQYQNWATPTAMNKLLIDTYNNKNQLLSKKSYDFIWKIMRETTTGSN
                     RLKGQLPKNTIVAHKTGTSGINNGIAAATNDVGVITLPNGQLIFISVFVAESKETSEIN
                     EKIISDIAKITWDYYLNK"
                     /name="extended-spectrum class A beta-lactamase VEB-17"
     gene            6686..7219
                     /gene="aadB"
                     /locus_tag="PUP03_RS00045"
                     /old_locus_tag="JALKJMPE_00008"
     CDS             6686..7219
                     /gene="aadB"
                     /locus_tag="PUP03_RS00045"
                     /old_locus_tag="JALKJMPE_00008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_000381802.1"
                     /translation="MDTTQVTLIHKILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVMEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRLACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            7281..7556
                     /locus_tag="PUP03_RS00050"
                     /old_locus_tag="JALKJMPE_00009"
     CDS             7281..7556
                     /locus_tag="PUP03_RS00050"
                     /old_locus_tag="JALKJMPE_00009"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223847821.1"
                     /translation="MAGLVKRYGDDLSKCDFIAAMKLASKGREPDEIGKAMAEASPAIM
                     ERKTGHEADYIKRTVQKVMELPQVQEARAELAKQAEKQQYRGPTLG"
                     /name="hypothetical protein"
     gene            7613..7819
                     /gene="urfF"
                     /locus_tag="PUP03_RS00055"
                     /old_locus_tag="JALKJMPE_00010"
     CDS             7613..7819
                     /gene="urfF"
                     /locus_tag="PUP03_RS00055"
                     /old_locus_tag="JALKJMPE_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_112928753.1"
                     /translation="MKDQHDKRTGNLLATPNAIRQARFAEQQRAMGRKARKIWATDAEA
                     EALRLYLEELRAAQGGGSDPASA"
                     /name="urfF"
     ARG             5008..5555
                     /name="aac(6')-IIa"
                     /gene="aac(6')-IIa"
                     /gene_name="aminoglycoside N-acetyltransferase AAC(6')-IIa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.45"
                     /coverage="99.46"
     ARG             6687..7216
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             5643..6538
                     /name="blaVEB-17"
                     /gene="blaVEB-17"
                     /gene_name="extended-spectrum class A beta-lactamase
                     VEB-17"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             4906..5558
                     /name="AAC(6')-IIa"
                     /gene="AAC(6')-IIa"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.45"
                     /coverage="117.93"
     Typing          2590..2676
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.864"
                     /coverage="100.0"
                     /evalue="1.13e-36"
     Typing          2..840
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.881"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2739..4846
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaccc ataagcctat cgacattctg gcgagcttca cagagctacc gccaccgatt
       61 gactacgtac tgcccaacat ggtggccggc accgtggggg cgctggtgtc ccccggtggg
      121 gccggcaagt ccatgctggc cctgcaactg gccgcacaga tcgcaggcgg ccccgacctg
      181 ctggacgtgg gcgagttgcg caccggcccg gtgatctacc tgcccgccga agacccgccc
      241 accgccatac atcaccgcct gcacgcattg ggggaacacc tgaccgacga gcaacggcaa
      301 gccgtggctg acggtctgct aatccagccg ctgatcggca gcctacccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgagcc gctgagggac gccgcttgat ggtgctggat
      421 accttgcgcc ggttccacat cgaggaagag aacgccagcg gccccatggc gcaagtgatt
      481 ggccgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggatcgtcc
      601 gtgctggtgg ataacatccg ctggcaatcg tacctgtccg gcatgaccac cggtgaggcc
      661 gaggaatggg gcgtagacga cacgcagcgg ccctatttcg tccgctttgg agtcagcaag
      721 gccaactatg gggcaccgtt caaagaccgc tggttcaggc ggcatgacgg cggggtactc
      781 aagcccgccg tgctggaaaa gcagcacaag agcaaggggg tgcctcgtgg caaagcctaa
      841 gaacaaacac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcggcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatttgcg
     1021 catcctgcag gggctggtgg ccatggccgg gcctagtggt ctggtgctag agccagaacc
     1081 ggagaccccc ggcgggcagc agctccggct gttcctggaa cccaaatggg aagccgttac
     1141 cgctgacgcc atggtggtca agggtagcta tcgggcgctg gctcgtgaaa taggctatgc
     1201 caatatcgag gactcaaagc ccatcaggga ctgcattgaa cggctttgga aggtgtccat
     1261 catcgcccag aatggccgca agcggcaggg cttccggctg cttgccgagt acgccagcga
     1321 cgaggcagac gggcgcctgt atgtggccct gaaccccttg atcgcgcagg ccgtcatggg
     1381 tggcggccag catgtgcgca tcagcatgga cgaggtgcgg gcgctggaca gcgaaaccgc
     1441 ccgcctgctg caccagcggc tgtgtggctg gatcgacccc ggcaagaccg gcaaggcggc
     1501 catagatacc ctgtgcggct acgtctggcc gtcagaggcc agtggggccg ccatgcgcaa
     1561 gagacgccag cgggtgcgcg aggcgctgcc ggagctgatc gcgctgggct ggacggtgaa
     1621 cgagtatgcg gcgggtaagt acgacatcgc acggcccaag gcggcaggct ggctgacccc
     1681 tgcaataact gtcacgcccc cccgttaact gtcacgcccc cccgttaact gtcacgaacc
     1741 ccccgttaac tgtcacgccc cccgttaact gtcacgaacc ccccgttaac tgtcacgccc
     1801 ccaaacctgc aaacctagca ggggcggggg ctggcggggt gctgaaaaat ccatccatga
     1861 ttatctaaga ataatccact aggcgcggtt aacagcgccc tagtggggcg ctgtttgccc
     1921 ttgcccaaaa tgcccggcca gaggccggat aactgatcta tccgctgcgc taggctacac
     1981 accggcccca ccgctccgcg gtagggaaaa ggcgggccaa ggcccgcaaa cccaaacccc
     2041 atgcacagac gccacaggaa gccgcagacg cctttagccg ctttagcggc ctttacccct
     2101 gcccgcaggg cgggggcgcg tgtgaagccc cgcaggggcc atgctggcca ccttgcgctt
     2161 ggctatctcc gccttgatgg tcgcgcccgc ctctcgcatc tcttgccgca gggcttcgat
     2221 ctcggcaggt gtcagccggt agaagctcac ccttgcccct cttgcggctc cggctggcca
     2281 tgctcctgtg gctgctattc ctgcttgggt ggcagtccga acagcgcccg gtcatggtcg
     2341 cgctctaggt agctgtccag cccatcgagc aagcgctgtt ctggccactc gccgctttcg
     2401 actttgccga gtatccacgc gcctaccagc accttgcgcc gggtgtcctc cttgcgcttt
     2461 tgctgctgct cccgtgccct gacccgctgg atctcgacgt tgatccgtgc ccgttgctct
     2521 tccagcttgg cgagccgttc cgctgctttt gccatgttga cattcccctt ggttatgtgc
     2581 ttgccatcat gctatcattg gagcacagac accgcaaacc ccgaccgtca ttctaacggg
     2641 agggcgcact taccggtttc tcttcgagaa actgccctgc gggcacccct ttggggctgt
     2701 gcgctctccg agggccattg catggagcta aacccctatg gccatctatc accttacggc
     2761 caagaccggc agccggaacg gcggccagtc ggccaaggcg aaagccgatt acatccagcg
     2821 ggagggccgt tactcccgcg accgtgacga ggtgctgcac actcagtccg gccacctgcc
     2881 cgagtgggcc gagcgccccg ccgactactg ggatgctgcc gacctgtatg aacgcgccaa
     2941 tgggcggctg ttcaaagagg ttgagtttgc cctgccggtc gaactgaccc tagatcagca
     3001 gcgggagctg gtggacgaat tcgcccggca tctgaccgag ggcgagcgcc tgccgtatac
     3061 gctggccatt catgccggta aaggtgagaa cccccattgc cacttgatga tctctgagcg
     3121 gaagaacgac ggtatcgagc gcccggctga ccagtggttc aagcggtaca acgccaaaca
     3181 gccagagcgg ggcggggcgc agaagtccga gagcctgaag cccaagggat ggcttgagca
     3241 gacccgcgag gcatgggccg accatgccaa ccgggcatta gagcgggctg gtcacgacgc
     3301 ccgcattgac catagaacac ttgaggcgca gggcatcgag cgcctgccgg gcatccatct
     3361 tggccccaac gtggtggaga tggagggccg gggcatccgc accgagcggg ccgacaccgc
     3421 cctagccatc gacacggcca acggccagat catcgactta caagaatacc gggaggttat
     3481 cgagcatgag cgcgatagac agagtgaaga agtccagagg catcaacgag ttagcggagc
     3541 agatcgaacc gctggcccag agcatggcga cactggccga cgaggcccgg caggtcatga
     3601 gccagactca gcaggccagc gaggcgcagg cagcggagtg gctgaaagcc caacgccaga
     3661 caggggcggc atgggtggag ctggccaagg agttgcggga ggtagccgcc gaggtgagca
     3721 gcgccgcgca gagcgcccgg agcgccgccc gtggctggac atggaagttg tgggccgggg
     3781 tcttgatcgc ttccgtgacg cctattctgg cgctgctgat cgcatcatgg ctttggctgg
     3841 agccgcaaat catcgaacaa caggggggta tatggctggt tttcaagctc aagtaaaggg
     3901 tgaccgcacc gcgcaagcca tcgcccggca gctcaaggcc atgggctgcg accggtacga
     3961 catcggcata cgggatgccg ccagcggcaa gatgatgaac cgggaatggt cagccgccga
     4021 agtgctccag aacacgccat ggctcaagcg gatgaatgcc cagggcaatg acgtgtatat
     4081 caggcccgcc gagcaggagc ggcatggttt ggtgctggtg gacgatctca gtgagttcga
     4141 cttggatgac atgaaagccg agggccggga gccggcgctg atcgtggaga ccagccccaa
     4201 gaactatcag gcatgggtaa aagtggctca ggacgcccca gcaggtcaca ggggcgtaat
     4261 cgcccggaag ctggcccgcg agtacgacgc cgacccggcc agcgccgaca gccgccacta
     4321 tggtcgcttg gcgggcttca ccaaccgcaa ggacaagcac accacccgca ccggttatca
     4381 gccgtgggtg ctgctgcgtg aatccaaggg caagaccgcc accgcagggc cggagctgat
     4441 gcagcaggcc gggcaggtgc tggacagcat taagcgccaa caggaacgga cggcacgact
     4501 ggccgagatc accgccccgc ggagtgtgcg ccggtatcgc cgcagcgccg tggacgacta
     4561 ccgcagcgaa atggccgggt tggtcaaacg cttcggtgac gacctcagca agtgcgactt
     4621 cattgcggct atgaagctgg ccagcaaggg ccgggagccg gacgagatcg ccaaggccat
     4681 ggccgaggcc agccccgcga tcatggagcg caaggcaggc catgaggccg attacatcaa
     4741 gcgcacggta cagaaagtga tggagctgcc gcaggtgcag gaggcccgcg ccgagctggc
     4801 gaagcagacc cagaagcagc gcagtcgggg gccagattta agtatgtaat gcttgcgtta
     4861 gcgctaactg gtggtatact gttagctcta atggagggtt tgagatgaaa gaccaacacg
     4921 acaagcgtac cggcaacctg ctggccaccc ctaacgctat acgacaggcc cgctttgccg
     4981 aacagcagcg agccatgggc cgcaaggccg cgagcacccc ccccataact cttcgcctca
     5041 tgaccgagcg cgacctgccg atgctccatg attggctcaa ccggccgcac atcgttgagt
     5101 ggtggggtgg tgacgaagag cgaccgactc ttgatgaagt gctggaacac tacctgccca
     5161 gagcgatggc ggaagagtcc gtaacaccgt acatcgcaat gctgggcgag gaaccgatcg
     5221 gctatgctca gtcgtacgtc gcgctcggaa gcggtgatgg ctggtgggaa gatgaaactg
     5281 atccaggagt gcgaggaata gaccagtctc tggctgaccc gacacagttg aacaaaggcc
     5341 taggaacaag gcttgtccgc gctctcgttg aactactgtt ctcggacccc accgtgacga
     5401 agattcagac cgacccgact ccgaacaacc atcgagccat acgctgctat gagaaggcag
     5461 gattcgtgcg ggagaagatc atcaccacgc ctgacgggcc ggcggtttac atggttcaaa
     5521 cacgacaagc cttcgagaga aagcgcggtg ttgcctaaca actcattcaa gccgacgccg
     5581 cttcgcggcg cggcttaatt caggtgttag cggtaattta accagatagg agtacagaca
     5641 tatgaaaatc gtaaaaagga tattattagt attgttaagt ttatttttta caattgcgta
     5701 ttcaaatgct caaactgaca acttaacttt gaaaattgag aatgttttaa aggcaaaaaa
     5761 tgccagaata ggagtagcaa tattcaacag caatgagaag gatactttga agattaataa
     5821 cgacttccat tacccgatgc aaagcgttat gaaatttccg attgctttag ccgttttgtc
     5881 tgagatagat aaagggaatc tttcttttga acaaaaaata gagattaccc ctcaagacct
     5941 tttgcctaaa atgtggagtc cgattaaaga ggaattccct aatggaacaa ctttgacgat
     6001 tgaacaaata ctaaattata cagtatcaga gagcgacaat attggttgtg atattttgct
     6061 aaaattaatc ggaggaactg attctgttca aaaattcttg aatgctaatc atttcactga
     6121 tatttcaatc aaagcaaacg aagaacaaat gcacaaggat tggaataccc aatatcaaaa
     6181 ttgggcaacc ccaacagcga tgaacaaact gttaatagat acttataata ataagaacca
     6241 attactttct aaaaaaagtt atgattttat ttggaaaatt atgagagaaa caacaacagg
     6301 aagtaaccga ttaaaaggac aattaccaaa gaatacaatt gttgctcata aaacagggac
     6361 ttccggaata aataatggaa ttgcagcagc cactaatgat gttggggtaa ttactttacc
     6421 gaatggacaa ttaattttta taagcgtatt tgttgcagag tccaaagaaa cttcggaaat
     6481 taatgaaaag attatttcag acattgcaaa aataacgtgg gattactatt tgaataaata
     6541 aaaaactacc gctaacactg gctcataggc aatggcgggt tgaagtgcaa tttgcaaagt
     6601 cggtagcccg cccgagcgtt ttctcggttt gacaggaaag gctcacgcaa accgccactg
     6661 cccatagccc aaaccgttag gccgcatgga cacaacgcag gtcacattga tacacaaaat
     6721 tctagctgcg gcagatgagc gaaatctgcc gctctggatc ggtgggggct gggcgatcga
     6781 tgcacggcta gggcgtgtaa cacgcaagca cgatgatatt gatctgacgt ttcccggcga
     6841 gaggcgcggc gagctcgagg caatagttga aatgctcggc gggcgcgtca tggaggagtt
     6901 ggactatgga ttcttagcgg agatcgggga tgagttactt gactgcgaac ctgcttggtg
     6961 ggcagacgaa gcgtatgaaa tcgcggaggc tccgcagggc tcgtgcccag aggcggctga
     7021 gggcgtcatc gccgggcggc cagtccgttg taacagctgg gaggcgatca tctgggatta
     7081 cttttactat gccgatgaag taccaccagt ggactggcct acaaagcaca tagagtccta
     7141 caggctcgca tgcacctcac tcggggcgga aaaggttgag gtcttgcgtg ccgctttcag
     7201 gtcgcgatat gcggcctaac aattcgtcca agccgacgcc gcttcgcggc gcggcttaac
     7261 tcaggtgtta ccgcagcgaa atggccgggc tggtcaagcg gtacggtgac gacctcagca
     7321 agtgcgactt tatcgcggcc atgaagctgg ccagcaaggg ccgggagccg gacgagatcg
     7381 gcaaggccat ggccgaggcc agccccgcca tcatggagcg caagacgggc catgaggccg
     7441 attacatcaa gcgtacggtg cagaaggtga tggagctgcc gcaggtgcag gaggcccgcg
     7501 ccgagctggc gaagcaggcc gagaaacagc agtaccgagg gccgacatta ggataaaaca
     7561 cttgcattag agctaatgcc tgttatggtg ttagctctaa tgcggaggaa atatgaaaga
     7621 ccaacacgac aagcgtaccg gcaacctgct ggccaccccc aacgctatac gacaggcccg
     7681 ctttgccgag cagcagcgag ccatgggccg caaggcccgg aagatatggg ccacagacgc
     7741 agaggccgag gcgctgcggc tgtatctgga agaactcagg gcggcgcagg gcgggggtag
     7801 tgaccccgcc agcgcctaac caccaaccgc ctacacagga ggcaaatca
//
