LOCUS       NZ_OP242235            10560 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain LA069 plasmid pLAO42, complete sequence.
ACCESSION   NZ_OP242235
VERSION     NZ_OP242235.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363342
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10560)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10560)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Sequencing Technology             :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028600195.1-RS_2024_03_23
            Annotation Date                   :: 03/23/2024 03:12:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 19
            CDSs (total)                      :: 19
            Genes (coding)                    :: 15
            CDSs (with protein)               :: 15
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242235.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA069"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO42"
     gene            join(10502..10560,1..175)
                     /locus_tag="PUP09_RS00005"
     CDS             join(10502..10560,1..175)
                     /locus_tag="PUP09_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_152065882.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQLVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(660..1079)
                     /locus_tag="PUP09_RS00010"
                     /old_locus_tag="HOJEAHOA_00003"
     CDS             complement(660..1079)
                     /locus_tag="PUP09_RS00010"
                     /old_locus_tag="HOJEAHOA_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_032189070.1"
                     /translation="MRRTGNKLSLIAMITATVALTACTPKGSVELHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(1160..1597)
                     /locus_tag="PUP09_RS00015"
                     /old_locus_tag="HOJEAHOA_00004"
     CDS             complement(1160..1597)
                     /locus_tag="PUP09_RS00015"
                     /old_locus_tag="HOJEAHOA_00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_174218559.1"
                     /translation="MGHCHFMAWHTLQDALKMTGRSRSQFYRDMRAGLVSYRTSQDGRR
                     EFETSELIRAYGELKQDETPERHSAGQAENPHVQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRHQEAEREQLQNEIAQLRQALEQEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            1857..3416
                     /locus_tag="PUP09_RS00020"
                     /old_locus_tag="HOJEAHOA_00005"
     CDS             1857..3416
                     /locus_tag="PUP09_RS00020"
                     /old_locus_tag="HOJEAHOA_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_044166483.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGCGSGGGTGGSKSES
                     SAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRHNS
                     THPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQVE
                     TERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSSIHA
                     RDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGDEM
                     EEKQKQVTASETRLNQISSEINGIQKAISQANNKRSTAVSRIHDAEDNLKIAQTNLLNS
                     QIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVLNK
                     KFSKADRDAIFNALESVKYEDWAKYLDQFAKYLKITGHVSFGYDVVSDILKIKDTGDWK
                     PLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTINEVL
                     GI"
                     /name="colicin-like pore-forming protein"
     gene            complement(3413..3754)
                     /locus_tag="PUP09_RS00025"
                     /old_locus_tag="HOJEAHOA_00006"
     CDS             complement(3413..3754)
                     /locus_tag="PUP09_RS00025"
                     /old_locus_tag="HOJEAHOA_00006"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="WP_000058755.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 family microcin immunity protein"
     gene            complement(<3799..>4469)
                     /locus_tag="PUP09_RS00030"
                     /pseudo=""
     CDS             complement(<3799..>4469)
                     /locus_tag="PUP09_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(4594..>4896)
                     /locus_tag="PUP09_RS00035"
                     /pseudo=""
     CDS             complement(4594..>4896)
                     /locus_tag="PUP09_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008826.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            complement(4812..5084)
                     /locus_tag="PUP09_RS00040"
                     /old_locus_tag="HOJEAHOA_00009"
     CDS             complement(4812..5084)
                     /locus_tag="PUP09_RS00040"
                     /old_locus_tag="HOJEAHOA_00009"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000124095.1"
                     /translation="MARSAKPRKRKPAPQRSKLPRYVVKLHPDDFFDEEDAEVLHFDNF
                     DDAVECCADLGIPFFTFFTNWANSGTGLPRQWLTGHRASTLLLMQ"
                     /name="hypothetical protein"
     gene            5103..5318
                     /locus_tag="PUP09_RS00045"
     CDS             5103..5318
                     /locus_tag="PUP09_RS00045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072006587.1"
                     /translation="MTLSFFITVIPGHDKPQPPGCLLDNLRELTDFYNSIGARIKETWI
                     ENGERVFQETSNGMVRLGRRRCTITY"
                     /name="hypothetical protein"
     gene            5595..6122
                     /locus_tag="PUP09_RS00050"
                     /old_locus_tag="HOJEAHOA_00010"
     CDS             5595..6122
                     /locus_tag="PUP09_RS00050"
                     /old_locus_tag="HOJEAHOA_00010"
                     /inference="COORDINATES: protein motif:HMM:NF015505.4"
                     /GO_function="GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="WP_152065880.1"
                     /translation="MDLIFKYKQATFLRAQQIDRNFHSGLCFIMCIDYIYNKKHNLPCD
                     FLYFCKTWQFTSRHRALLYLYQSGKYSGDPYLMKNGRLTLKYLRKAEHPIEIYLLTQGC
                     YIINISLDQGTKAHAVAYIKKIGETLFFDPNHGEYNIKNKLNLLDFLKREYSTRVDYIS
                     IYQVTEPVYHSV"
                     /name="YopT-type cysteine protease domain-containing
                     protein"
     gene            6298..6804
                     /locus_tag="PUP09_RS00055"
                     /old_locus_tag="HOJEAHOA_00011"
     CDS             6298..6804
                     /locus_tag="PUP09_RS00055"
                     /old_locus_tag="HOJEAHOA_00011"
                     /inference="COORDINATES: protein motif:HMM:NF013676.4"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_249403119.1"
                     /translation="MVPPSVILKKLAASPRQNRLNLALREMGRIERSIFICDWLLDTRL
                     RRRSHAILNKGESRHALARAIFLHQLGEFRNRAAETMAHRASGLNLVVNAIILWNTVYL
                     SRAVDYIRSQGIIIPEELLSSVSPLPWGRISLTGDYLWNEIDHPLERYRPLRTNRFNSK
                     NFTFP"
                     /name="transposase"
     gene            <6838..7221
                     /locus_tag="PUP09_RS00060"
                     /old_locus_tag="HOJEAHOA_00012"
                     /pseudo=""
     CDS             <6838..7221
                     /locus_tag="PUP09_RS00060"
                     /old_locus_tag="HOJEAHOA_00012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /name="colicin-like pore-forming protein"
     gene            complement(7218..7508)
                     /gene="cki"
                     /locus_tag="PUP09_RS00065"
     CDS             complement(7218..7508)
                     /gene="cki"
                     /locus_tag="PUP09_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            7538..7669
                     /locus_tag="PUP09_RS00070"
     CDS             7538..7669
                     /locus_tag="PUP09_RS00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(7864..8007)
                     /locus_tag="PUP09_RS00095"
     CDS             complement(7864..8007)
                     /locus_tag="PUP09_RS00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(8675..8866)
                     /locus_tag="PUP09_RS00075"
     CDS             complement(8675..8866)
                     /locus_tag="PUP09_RS00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8934..9257
                     /locus_tag="PUP09_RS00080"
                     /old_locus_tag="HOJEAHOA_00014"
     CDS             8934..9257
                     /locus_tag="PUP09_RS00080"
                     /old_locus_tag="HOJEAHOA_00014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            9466..>9903
                     /locus_tag="PUP09_RS00085"
                     /pseudo=""
     CDS             9466..>9903
                     /locus_tag="PUP09_RS00085"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            9935..10495
                     /locus_tag="PUP09_RS00090"
                     /old_locus_tag="HOJEAHOA_00015"
     CDS             9935..10495
                     /locus_tag="PUP09_RS00090"
                     /old_locus_tag="HOJEAHOA_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     Typing          8503..8590
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(7936..8492)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9248..10530
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.533"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 gcagcggctg aaagactggg agagcgcctt cacggatttg cagcatatgt tcgagctgac
       61 acgacaggag aacgggcaac tgagggaaca gtgcgacgac ttgagtcagc tggtgcagag
      121 attagccggg caggtggacc acttgagtcg gttatttatc acgaacagca gataagggat
      181 gaacaggaaa ggcaggcacg cctggaacgg gagcgtgcgc ggtcctggca tgaagagcag
      241 gaacgggaag cacgggtatg gcgtggcccg tcaataggga tgtgacggtg agttgttccg
      301 ggaggttgcc agatggtacc ggaacgcgtt aaaaacgcat ccgggcgcga ttctggcgtg
      361 tcgtttttgt tgctgtgttt tgcctctttg ccagatcgcg ctcattcctc agcctcatcc
      421 cgcgatgtct gcgtatgtca tcaactgaaa agatggaaaa aaaacagtgt catgaggttt
      481 acgcaggcaa aaccagcgtt attcacatgg ctgaatagct atgcatacgg ttaaaattta
      541 tcaggtgcga tcgcggcagc ttttcgggtg gtttgttgcc atttttacct gtcagcagcc
      601 gtgatcgcgc tgaacgcatt tcagcggtgc gtacaattaa gggattatgg taaatccact
      661 tactgtcttc cctcatagcc atcgagataa accgcagaaa tcgtctcagc cagcagcctg
      721 gattttttcg gggtaaggtc tttgctgcgg taatcagtac ccgcaaattg agttgtgccc
      781 cggagtgagt ttaaaaattc ctggctgtga aactgctgaa tgcgttgctg cacatcactc
      841 cgtgattttc ctgtcgcttt atcctttgct cccatatccc agaactgccg gaaaaaaggc
      901 accatcattc gtgttgtgtc ggctttttgg gtggaaaagt tgggatcaaa accgtcatca
      961 gaagcatata cgtaatgccg ggtatgtagt tccacgctgc cctttggggt acaggctgtg
     1021 agagctactg ttgctgttat catggcgata agagaaagtt tgtttccggt acgtctcatg
     1081 agagtctccc taccttaaag tatttatttt cggtgagtta ttatcagaag actattttat
     1141 catcagtcca tgacagcgtt cagcgaccaa acaaccggga ccagaatccc cgtttctttt
     1201 cctgctccag cgcctgcctg agctgggcta tctcattctg tagctgttcc cgttctgctt
     1261 cctggtgccg gcgctccgta tcctgggctt gtttatcctc aagcatcagc gtcaggcatt
     1321 gtttcagctc attcagctcc cggagaatgc gttctgtctg ctgaacatgt ggattttctg
     1381 cctgtcccgc actgtgcctt tctggtgtct catcctgctt taattcgccg tatgcccgga
     1441 tcagttcact ggtttcaaac tcccgtcgtc cgtcctggct ggtacggtaa ctgacaaggc
     1501 ctgcgcgcat atcacggtaa aactgtgaac gcgatctgcc tgtcattttc agtgcgtcct
     1561 ggagtgtgtg ccatgccatg aagtgacagt gtcccattgg tgtctcatat catggtttca
     1621 gtaaaacata ctgaggccgg agaacggtaa tgtttgtgct ggtttttgtg gcatcgggtg
     1681 gaaatagcac gtggtgtggg tggctgtttt tttgatcgtt ttcacaaaaa tgaaaatcca
     1741 cagggttgac agggaaaatg cagcagcgta attttaagct gtatataaaa ccagtggtta
     1801 tatatacagt atattgcttt taactatttg tttttaatgt caaagaggat tttataatgg
     1861 aaacagctgt agcgtactat aaagatggtg ttccttatga tgataagggg caggtaatca
     1921 ttactctttt gaatggtaat ccagacgggt gtggctctgg cggtggaact ggaggtagca
     1981 aaagtgaaag ttctgcagcc attcatgcca cagctaaatg gtctactgct caattgaaga
     2041 aaacgcaggc agaacaggct gcccgagcaa aagctgccgc agaagcacag gctaaagcaa
     2101 aagcaaaccg ggatgcgctg actcaacatc tgaaggatat tgtgaatgag gcgcttcgcc
     2161 ataattccac tcatccggag gttattgacc tggctcatgc caataatgca gcgatgcagg
     2221 cagaagcaga gcggttgcgc cttgcaaaag cagaagaaaa agctcgtaaa gaagcggaag
     2281 ctgcggaaaa ggcttttcag gaagcagaac aacgacgtaa agagatagag aaggagcagg
     2341 ttgaaacaga acgccaattg aaactggctg aggatgaaga gaaacgcctg gcagcattga
     2401 gtgaagaggc ccgggctgtg gaggtggcac aaaaaaatct tgctgctgca caatctgagc
     2461 tggcgaaagt ggatgaagag attaatacgc tcaatacccg tttaagctcc agtattcatg
     2521 cccgtgatgc agaaacgaat acgctgtccg gaaaacgaaa tgagttggat caggcatctg
     2581 ctaaatataa agaactggat gaaagggtta aactgttatc tcctagggcg aatgatccgc
     2641 ttcagagtcg tccttttttt gaggccacca gactacgggc gagagctggt gatgagatgg
     2701 aggaaaaaca aaagcaggta acagcatcag aaacgcgtct taaccagatt agctctgaga
     2761 taaatggaat ccaaaaggct atttctcagg ctaataataa gcgaagtaca gcagtttcac
     2821 gtattcatga tgctgaagat aatttgaaaa tagcacagac taatctcctg aactcgcaga
     2881 ttaaggatgc tgtggatgca acagttagct tttatcaaac gctatctgaa aaatatggtg
     2941 aaaaatattc aaaaatggca caggaacttg ctgataaatc taaaggtaag aaaatcagca
     3001 atgtgaatga agctctagct gcttttgaaa aatacaagga tgttttaaat aagaaattca
     3061 gcaaagcaga ccgtgatgca attttcaatg cactggagtc ggttaagtat gaagactggg
     3121 ctaagtattt agatcagttt gctaagtact tgaagattac gggacatgtt tcttttggat
     3181 atgatgtggt atctgatatc ctaaaaatta aggatacagg tgactggaag ccactatttc
     3241 ttacattaga gaagaaagct gtagatgctg gagttagtta tgttgttgtt ttacttttta
     3301 gtgtgcttgc tggaactaca ttaggtatct gggggattgc tattgttaca ggcattctgt
     3361 gtgcctttat tgataagaat aaacttaata ctataaatga ggtgttgggt atttaattat
     3421 tctttattga tataaacagt cctagcaatc caaatgggat tgctaggacc aaacaaagta
     3481 gattgtatag cataaatagg tttaattttg ccacgggggc attatttagg ttttttcttt
     3541 ttctgaaaaa atctttcttt atgaacttaa aaaatatgtg ttcaatagca tattttgaat
     3601 atgggcatag aatagtgctg atcactattg cgtatagcat cttatctgac gctaggaaat
     3661 aatacccttc gttgtttttt gttataagat atatatatat aagtgcgcag tataggccaa
     3721 acaaaatatt ttttatatag tatcttaaac tcataaatta aacctcgtta tatattcttt
     3781 tcattttata aggatcgagt tatctgtcag ccactggatg gactgttccg gtactttatc
     3841 gccaaagcgt ttttctatcg ctcctaacat cacatcctgc actgtcgctt tatcgtaacc
     3901 tcccgtggcg cggctgatct ccagcggctt cgcgatctgc gtaatcagga tgaacgtgct
     3961 cgcggttttc gccatcatcg caaccgaact caaagccatc ggaacaccaa cgctggtcgc
     4021 tttccttcac cgatattcgg cctttatgtt cccgctgccg tcgtgatgtt tgtcatgtaa
     4081 caacaacaga ctattttcgc tcattatccg gtaaacacgc tttgcattca cgaacggtag
     4141 tccttcattt cgggactgcc gcctgagtat cgcccagaca cggcgatacc cgtatgctgg
     4201 catatcgcca atgatatcca ggatgcgcga taagacttct gcatccgcag cctcatcgcg
     4261 cctctgacgc cgtttatctg gcttgttatc agatgccttc atccgtaatg ttaactgcgc
     4321 acgtgacacg cccagaacac ggcttaccgg ggctattcct tgtccgaagg taacaagggc
     4381 acatgcgcta tccattttcg cgattgtgca tactccaccg cttctttgag gatctcaact
     4441 tccatggttt tcttacccag taaacgctgc cagctcaata aggatcgagt tatgaggaaa
     4501 agattttttg tgggaatatt cgctataaac ctccttgttg gatgtcaggc taactatata
     4561 ggggttccgt gggcatttct gcgataacac acgctaagga aaagtgaagt tctttggatt
     4621 gaagcgatta gaacgaagtg gcctgtatcg ttcaagggga tggtcaattt cgttccacag
     4681 gtaatctcca gtgagcgaga tatgacccca tggtagtggt gatacactgg acaagagctc
     4741 ttccggtata atgatcccct ggctgcgaat gtaatcaaca gcccgactga gatagaccgt
     4801 gttccacaga attattgcat taacaacaag gttgaggccc gatgccctgt gagccattgt
     4861 ctcggcagcc ctgttccgga attcgcccag ttggtgaaga aggtaaagaa cggaataccc
     4921 aggtcagcgc agcactcaac ggcatcgtca aaattatcaa agtgcagaac ttcagcgtct
     4981 tcttcgtcaa aaaaatcatc cggatgaagt ttcacaacat agcggggaag tttgcttctt
     5041 tgtggtgcgg gtttgcgttt acggggttta gctgaacggg ccatataact acctgaaaga
     5101 caatgacatt atcttttttt ataacggtaa ttccaggcca tgacaagccg cagccgccag
     5161 gctgcctact cgataatcta cgggaattaa ctgattttta taattccatc ggcgctagaa
     5221 ttaaagagac ctggatagaa aatggagaaa gagtatttca agagacatca aatggaatgg
     5281 ttaggctggg aagacgcaga tgtacaatta catactaatt aaaaaattta attttgagtg
     5341 cgtaagttgt ataattgatg aaagcatccg gtaaatttgt cacagacgtg aggaggctgc
     5401 ggatacggaa tagattgagc ccttgaaaca ccatgcaatg gatgtatctt aaaataagag
     5461 gtaggtttgg atatatgtct aacactagtt ctaattttga gatgactgga gctttactgc
     5521 ggcaggcagg agcttcataa acataaaact cctcagaaaa attgccatgc aatattgaat
     5581 tagagtataa atacatggat ttgatattta aatacaagca agcgactttt cttcgggcac
     5641 aacaaatcga tagaaatttc catagcggtt tatgttttat aatgtgtatt gactatatat
     5701 acaataaaaa gcacaatctt ccttgtgatt ttttatattt ttgtaaaacc tggcagttta
     5761 cttcaagaca tcgggcgttg ttatatttat atcagagcgg aaaatattct ggagatcctt
     5821 atttaatgaa aaatggaaga ttgacactga aatatttgag gaaagcagaa catccaatag
     5881 aaatatatct tttaactcag gggtgttaca ttataaacat ttcactagat caaggaacta
     5941 aagctcatgc agtagcttat ataaaaaaaa taggggagac tttatttttt gaccctaatc
     6001 atggagagta caatataaaa aacaaattaa atttattgga ctttcttaag agagagtaca
     6061 gtacaagagt agattatata tctatttatc aagtgaccga gcctgtttac cattccgtgt
     6121 aaatgctttt tcttaaaagt gaccgtcaag gcggtcacca aactcgataa taaagcggct
     6181 cattgtcatt cgccagttac tctgcggcat tgtccatttc tgtgacgcgt cctggattgc
     6241 ctaccagaac agcactggga tgaagttctg cggcttaagg cctcgatcgg cgctggtctg
     6301 gtaccaccat cggtaatact gaaaaagctg gcagcatctc cgagacaaaa caggctcaat
     6361 ctggccctgc gtgaaatggg acgtattgaa cgctcaattt ttatctgtga ctggttgctt
     6421 gacactcggt tacgtcgcag atcacacgca atcctcaaca aaggcgaaag ccgccatgca
     6481 cttgcccgcg ctatttttct tcaccaactg ggcgaattcc ggaacagggc tgccgagaca
     6541 atggctcaca gggcatcggg cctcaacctt gttgttaatg caataattct gtggaacacg
     6601 gtctatctca gtcgggctgt tgattacatt cgcagccagg ggatcattat accggaagag
     6661 ctcttgtcca gtgtatcacc actaccatgg ggtcgtatct cgctcactgg agattacctg
     6721 tggaacgaaa ttgaccatcc ccttgaacga tacaggccac ttcgtactaa tcgcttcaat
     6781 tcaaagaact tcacttttcc ttagcgtgtg ttatcgcaga aatgcccacg gaacccctat
     6841 aagaatgtac tggataagaa attcagtaag gttgatcggg atgctatttt taatgcgtta
     6901 gagtctgtta attatgatga gttatctaag aatttaacga agatatcaaa gtcgcttaaa
     6961 atcaccagtc gagtatcttt tctatatgat gttggttctg attttaaaaa tgcaatagaa
     7021 actggtaact ggcgaccttt gtttatcacg ctggagaaaa gtgcggtcga tgtaggtgtg
     7081 gcaaaaattg tggcattaat gtttagtttt attgttggtg tgcctcttgg tttctggggg
     7141 attgcaattg tcacaggtat tgtttcttct tacatagggg atgatgagtt gagcaaactt
     7201 aatgagttgt tagggattta atttctcttt agagtgtaat atatgaaact tggcaatcct
     7261 actgggattg ccattaataa ataaaacaat gcagataact tgcataagta ccagggttct
     7321 ttttttaact ttgtattttc tttgatatat ctagatatgg ttaatttaga gtatggatat
     7381 agcaatacat gtattgatga tataaatata aataacaaag gagtgttatc attgtcgtta
     7441 aatattaaaa taagaatcca tggtataagt gattcaggta aattatgtag gtagtatttt
     7501 aaatgcataa gtatttttac ttttgttgga aagaaatatg aaaataataa tttttctaat
     7561 tacaatatta ttcttgtcag gatgtcaagc aaactatatt cgtgatgtac aaggaggaac
     7621 cgttgcacca tcttcgtctt ctgaactgac ggggatcgcg gttcagtaga aaagatcaaa
     7681 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcctgtaaac gaaaaaaccg
     7741 cctggggagg cggtttagtc gaaggttaag tcagttgggg actgcttaac cgggtaactg
     7801 gctttagtgg agcacagata ccaaatgctg tccttccagt gtagccgcta taaggcttca
     7861 acttcaagag ttctgcgtat ctaaatccac tatccagtta ccaatggctg ctgccagtgg
     7921 cgttaagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7981 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8041 actgagatac caacagcgtg agctatgaga aagcgccacg cttcccaaag ggagaaaggc
     8101 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agccaccagg
     8161 gggaaacgcc tggtatcttt aagtcctgtc gggtttcgcc acctctgact tgagcgtcgg
     8221 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagctg cgtggccttt
     8281 tccgggttct tgttttttgc tcacatgttt cttttggtgt tatcccctga ttctgtggat
     8341 aaccgcatta ccgtctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgg
     8401 agcgagtcag tgagcgagga agcggaaaag cgcctggacg agcattttct ccttacgcat
     8461 ctgtgcggca tttcacaccc ggcatggtgc acttttcata caatcctcac tgatgccaaa
     8521 tgattaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     8581 acccgctaaa acccgctgac gcgccctgac gggcttgtct gctcccggca tccgctcaca
     8641 ggccagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatctccga
     8701 agcgtgcgag gcagctgcgg taaagctcgt cagcgtggtc gtgaagcgct tcgcagatgt
     8761 cagcctggtc gtcggcgtcc agttcattca gtttttccag cagggtcagc gtctggcttc
     8821 tgatgaagcg ggccatgtta agggcggttt tttcctgttt agtcacagtt ctgtttctcc
     8881 atgtgtgggg agtctgtcgg aggtttttaa tgataccggt gaaacgcgaa aggatgctca
     8941 cgatacgggt cacggatgag gaacatgcgc ggctgctggc acgttgtgag ggaaaacaac
     9001 ttgcgtcatg gatgcgtaag gtctgcctgg gggcaccgcc atcgaaaacg tcaggtcttc
     9061 cgacactggc accgccgctg ttgcgccagt tcgcttcggt cggcaataac ctgaatcaga
     9121 tagcccggaa gataaacagc ggacagtggt cggggcatga ccgggttcat gtggtggctg
     9181 cactgatggc catcggacgt gaactgagtg agctgaggga tgaggtccgt aaacagggag
     9241 aacgggatga tagttaagtt tcatgccagg ggaaaaggcg gtggcagtgg tccggttgat
     9301 tacctgctgg gccgggagcg taaccgggaa ggggcgagag ttctgcgggg tgctcccgaa
     9361 gaggtgcggg aactcatcga tgccacgccg tttgcgaaaa aatacacctc gggggttctg
     9421 tcttttgcag agcagaccct gccaccggga gagcgtgaaa gggtgatgga gagctttgag
     9481 cgggtactga tgcccggtct tgaaaaaaat cagtacagca tcctgtgggt ggagcaccag
     9541 gacaaggggc gtcttgagct gaattttgtc atcccgaata tggagctggc gagcggaaaa
     9601 cggctccagc cctattacga ccgagcggac cgtccgcgaa tcaatgcctg gcagacactg
     9661 gtgaatcacc attacgggct gcacgacccg aacgcaccgg agaacaggcg gacacttgtc
     9721 accccgaata atctgccgaa agcgaaacag gaggccgcag aagcgattac gaggggactg
     9781 gaggctcttt accatgccgg agcgctaaaa acgcgtcagg atgtcactga ggcgcttaca
     9841 gcggcaggtt ttgaggttgt gaggacaacc aggagcagta tcagtatcgc cgacccggag
     9901 ggtcggcgta atctcaggct gaaaggggcg ctgtatgagc aatcttttac aaatggggac
     9961 agacttcgag aagaaactga aagagcgtgc cgtctctaca gagaacatgc tgaacagcga
    10021 gttcaggaag ctcgaagcgt ctgtagacag ggaactgaac ttaaacgggc agaaaatcag
    10081 agacgctatc agcgcacaca cagcagcggt gaaagaacaa ctggagacgc tgaacagtgc
    10141 cgtgaacgcg cagttcagcg ccacagagaa cgaactggcc cggcggcagg aagagctgct
    10201 ctggaagctg gtcaaagggc ggatactgta cccgtcgctg acggcgcttt gcgtcacggg
    10261 cgggattttt ctggcgtcat gggggctgat tcagtggcag gaaagccgga ttgcggcgaa
    10321 catcctggct atcagggacc aggaagagac gctggcgaaa ctccgggaaa aaacatgggg
    10381 tgtgacgtat cacgagggca ggaacgggaa atttctggtg ttgccgtccg gggtgaaggg
    10441 ggaaaacaac tggacggtgg agaagaagaa cgcagtaaga ctggtcaggg agtaagtgag
    10501 tatgacagag ctggaaatgc acttactgaa cgccttagag caattgcagc aggactatac
//
