LOCUS       NZ_OP038303             8401 bp    DNA     circular CON 14-JAN-2024
DEFINITION  Shigella sonnei strain 201903956 plasmid p201903956-4, complete
            sequence.
ACCESSION   NZ_OP038303
VERSION     NZ_OP038303.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Recent and rapid emergence of extensively drug-resistant Shigella
            sonnei
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2022) Universite Paris Cite, Unite des Bacteries
            pathogenes enteriques, Centre National de Reference des Escherichia
            coli, Shigella et Salmonella, Institut Pasteur, 28 rue du Dr Roux,
            Paris F-75015, France
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Assembly Name                     :: p201903956-4
            Sequencing Technology             :: MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 02:42:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP038303.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="201903956"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /plasmid="p201903956-4"
     gene            complement(join(<8334..8401,1..121))
                     /locus_tag="OZD77_RS00005"
                     /pseudo=""
     CDS             complement(join(<8334..8401,1..121))
                     /locus_tag="OZD77_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(165..587)
                     /locus_tag="OZD77_RS00010"
                     /old_locus_tag="p201903956-4_00001"
     CDS             complement(165..587)
                     /locus_tag="OZD77_RS00010"
                     /old_locus_tag="p201903956-4_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            772..1212
                     /locus_tag="OZD77_RS00015"
                     /old_locus_tag="p201903956-4_00002"
     CDS             772..1212
                     /locus_tag="OZD77_RS00015"
                     /old_locus_tag="p201903956-4_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(1979..2128)
                     /locus_tag="OZD77_RS00020"
                     /old_locus_tag="p201903956-4_00003"
     CDS             complement(1979..2128)
                     /locus_tag="OZD77_RS00020"
                     /old_locus_tag="p201903956-4_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            2699..2857
                     /locus_tag="OZD77_RS00055"
     CDS             2699..2857
                     /locus_tag="OZD77_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            3317..4132
                     /gene="sul2"
                     /locus_tag="OZD77_RS00025"
                     /old_locus_tag="p201903956-4_00004"
     ARG             3318..4129
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4193..4996
                     /gene="aph(3'')-Ib"
                     /locus_tag="OZD77_RS00030"
                     /old_locus_tag="p201903956-4_00005"
     ARG             4194..4993
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            4996..5832
                     /locus_tag="OZD77_RS00035"
                     /old_locus_tag="p201903956-4_00006"
     CDS             4996..5832
                     /locus_tag="OZD77_RS00035"
                     /old_locus_tag="p201903956-4_00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            6138..6380
                     /locus_tag="OZD77_RS00040"
                     /old_locus_tag="p201903956-4_00007"
     CDS             6138..6380
                     /locus_tag="OZD77_RS00040"
                     /old_locus_tag="p201903956-4_00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(6412..7062)
                     /gene="tetR(A)"
                     /locus_tag="OZD77_RS00045"
                     /old_locus_tag="p201903956-4_00008"
     CDS             complement(6412..7062)
                     /gene="tetR(A)"
                     /locus_tag="OZD77_RS00045"
                     /old_locus_tag="p201903956-4_00008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            7168..8367
                     /gene="tet(A)"
                     /locus_tag="OZD77_RS00050"
                     /old_locus_tag="p201903956-4_00009"
     ARG             7169..8364
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             4997..5829
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(2164..2337)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          2261..2866
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cagcgaatgc cgggtaatac tcgtcaacga tctgatagag aagggtttgc tcgggtcggt
       61 ggctctggta acgaccagta tcccgatccc ggctggccgt cctggccgcc acatgaggca
      121 tgttccgcgt ccttgcaata ctgtgtttac atacagtcta tcgcttagcg gaaagttctt
      181 ttaccctcag ccgaaatgcc tgccgttgct agacattgcc agccagtgcc cgtcactccc
      241 actcttacat tgttcgcagt atctaggtac atgttattgc gtgcaccttc atgatgtata
      301 atgttattaa tgacagcaga aagatcgaca gaattaatat tcttcgtacc tgggacagtt
      361 atcgggtgtc ggccagtgat gagaccaaag gatgcagtat aagatgaacc actactatta
      421 tcatggccac cattaccacc accgttaaaa ctacctcccg aacttccaga gccgctatga
      481 ccatcattgt aggggtcatt tggtcccatg tgaccaaaac caccgacagt accgctattt
      541 aactctgtac tcataaaatt atcctctttg acttttaaaa acagcaaaaa ctgtatttat
      601 aacaatgtgt ttatatacag tattcgaaat actacgcccg tcaaaaccac accgtcaaca
      661 ggttttttgt gtatttttca cgcctcacgg gcagcctcag accttttgtg ttaaaacagt
      721 aatatgttct gctgttacaa tacggtacac acaggagaca ctgtcacttt tatggcatgg
      781 cacacagttc aagagacact gaaattaaca ggaaagtcgc gctcgcaatt atatcgtgac
      841 atggctaaag gccttgtcag ctatcgcaca ggaaaaaatg atcgccggga gttcgagacc
      901 agtgaactga ttcgggccta tggcgagctg cgcacttctg agacaccaaa aaggcacaag
      961 gagggacact ccaaatctgt cgttagccag gcggaaaatg ctcagctaga cgtcattcag
     1021 cagaaactgg acgctctgca gcagactgtt accctaatgc ttgaagacaa gatggcgcgg
     1081 gaagcagaaa accgcctgcg aaatgaggag agggaacaac tacaggccga aattagccgt
     1141 ctggctgacg cgctggcaca ggaaaaaaag cggggattct ggtcgcggct gtttcgctcc
     1201 ggttcagagt aacagtgcag gtgactctga tttaccataa catctgttat cagcaccgcc
     1261 tgagaggccc tcagggcgtt tctgacggga aaccggtaaa acaggcaaca aaccacccga
     1321 aaacccgcca gaaacgcgca ggctctgttt ttacggcatg catgagtatg cgcacaagtg
     1381 catagcgcgg aaattcacac cattcagccg gggtaagtcc tgtttccggc gttcttccca
     1441 tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc gtgaagcgtt gccggcgcag
     1501 ccggaaaacc cgctgtcagc acgttcagga agggggtgat gtacaaatgg cacagaggat
     1561 gaaagtgacg tatgagctga aggaatgatg taaaagtgat gtataagtga tgtaaaagtg
     1621 atgtataagt gatgtaaaag tgatgtatga actgaaggag cgatgtaatt ctgtagtcag
     1681 gaagaataac ccgttcatgc aggcatgaac gatacagtga ggaacgcttt ttttgtcacg
     1741 atctcccctt aacctgcgta aatcacccgc catggttgat tatttttcaa cctcaaaatg
     1801 gtcatacacc taacgcagcc gcgattttca tcgcaactgc atgaattaaa agaaaaaaaa
     1861 ggaatatttc gttttcacga gtaatacccg ggtattaaaa atccactgta attacatgaa
     1921 aataaagaaa taaaaggtaa taccggagta ttaccacgta cgcgggcaag cgaaaaagct
     1981 ataacgcttc ccggccgaca ggcgacccaa aatccacagg ggcatgcagg ttctcttcag
     2041 taacctgcgc cattaaatca aaaaggtcat attcgccggg aagctgcgta tcagtcgaat
     2101 ccagttgttc atcatattca gatggcatgg ggtgatatcc agtagctgag aggttgttag
     2161 cgggtttcgg ggcgcagccc tgaaccagtc atgtagcgct agcggagtgt atactggctt
     2221 actatgttgc cacgattgcg aaaatcagtg aagtgcttca tgtggcagga gaaaaaaggc
     2281 agcaccggcg cgtcagcaga atatgcgata caggagatat tccgcttcct cgctcactga
     2341 ctcgctacgc tcggtcgttc ggctgcggcg agcggtaccg gcttacggac ggggacggaa
     2401 atttcctgga agataccagg aagacactta gcaaagaaga gataaggcca cggcgaagcc
     2461 gtttttccat aggctccgcc cccctgacaa gcatcgcaaa atctgacgct caaatcagtg
     2521 gtggcgaaac ccgacaggac tataaagatc ccaggcgttt ccccctggta gctccctcgt
     2581 gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcggtt
     2641 tctcattcca cgcctgacac tcagttccgt gaaggcagtt cgctccaagc tggactgtat
     2701 gcacgaaccc cccgttcagt ccgaccgctg cgccttgtcc ggtaactatc aacttgagtc
     2761 caacccggaa agacacgaca aaacgccact ggcagcagcc attagtaaca ggagtgcacg
     2821 agaagataga ttaagaggtc ttgaagtaga ggcctgactg cggctacact gaaagaacag
     2881 ttttggtaat ggcggtctcg tgaaccctgt taccgggtta agcagttccc caactgactt
     2941 aaccttcgaa caaaccgcct ccccaggcgg ttttttcgtt tacagagcaa gagattacga
     3001 ttcattgaaa aggatctcaa gaagatcctt tgtagattac tatcaattaa tcttccgaac
     3061 atgacaccag tcactgttga ttacggttaa accgctccgt aacaggtttc cctcgtattg
     3121 gtgacttgca gcaccaaaga agcaaaaaag ataaatatat ctacttattg agagatgata
     3181 tatttatttc caaaatgcta cagatacatc atcatgaaac ggatcaccgc aaacaggtta
     3241 ctcgcctggg gattcccttt cgacccgagc atccgtatga gactcatgct cgattattat
     3301 tattatagaa gcccccatga ataaatcgct catcattttc ggcatcgtca acataacctc
     3361 ggacagtttc tccgatggag gccggtatct ggcgccagac gcagccattg cgcaggcgcg
     3421 taagctgatg gccgaggggg cagatgtgat cgacctcggt ccggcatcca gcaatcccga
     3481 cgccgcgcct gtttcgtccg acacagaaat cgcgcgtatc gcgccggtgc tggacgcgct
     3541 caaggcagat ggcattcccg tctcgctcga cagttatcaa cccgcgacgc aagcctatgc
     3601 cttgtcgcgt ggtgtggcct atctcaatga tattcgcggt tttccagacg ctgcgttcta
     3661 tccgcaattg gcgaaatcat ctgccaaact cgtcgttatg cattcggtgc aagacgggca
     3721 ggcagatcgg cgcgaggcac ccgctggcga catcatggat cacattgcgg cgttctttga
     3781 cgcgcgcatc gcggcgctga cgggtgccgg tatcaaacgc aaccgccttg tccttgatcc
     3841 cggcatgggg ttttttctgg gggctgctcc cgaaacctcg ctctcggtgc tggcgcggtt
     3901 cgatgaattg cggctgcgct tcgatttgcc ggtgcttctg tctgtttcgc gcaaatcctt
     3961 tctgcgcgcg ctcacaggcc gtggtccggg ggatgtcggg gccgcgacac tcgctgcaga
     4021 gcttgccgcc gccgcaggtg gagctgactt catccgcaca cacgagccgc gccccttgcg
     4081 cgacgggctg gcggtattgg cggcgctgaa agaaaccgca agaattcgtt aactgcacat
     4141 tcgggatatt tctctatatt cgcgcttcat cagaaaactg aaggaacctc cattgaatcg
     4201 aactaatatt ttttttggtg aatcgcattc tgactggttg cctgtcagag gcggagaatc
     4261 tggtgatttt gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg cacctgcttc
     4321 ccgccgcggt gagctcgctg gagagcgtga ccgcctcatt tggctcaaag gtcgaggtgt
     4381 ggcttgcccc gaggtcatca actggcagga ggaacaggag ggtgcatgct tggtgataac
     4441 ggcaattccg ggagtaccgg cggctgatct gtctggagcg gatttgctca aagcgtggcc
     4501 gtcaatgggg cagcaacttg gcgctgttca cagcctatcg gttgatcaat gtcagtttga
     4561 gcgcaggctg tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca atgccgtcaa
     4621 tcccgacttc ttaccggacg aggacaagag tacgccgcag ctcgatcttt tggctcgtgt
     4681 cgaacgagag ctaccggtgc ggctcgacca agagcgcacc gatatggttg tttgccatgg
     4741 tgatccctgc atgccgaact tcatggtgga ccctaaaact cttcaatgca cgggtctgat
     4801 cgaccttggg cggctcggaa cagcagatcg ctatgccgat ttggcactca tgattgctaa
     4861 cgccgaagag aactgggcag cgccagatga agcagagcgc gccttcgctg tcctattcaa
     4921 tgtattgggg atcgaagccc ccgaccgcga acgccttgcc ttctatctgc gattggaccc
     4981 tctgacttgg ggttgatgtt catgccgcct gtttttcctg ctcattggca cgtttcgcaa
     5041 cctgttctca ttgcggacac cttttccagc ctcgtttgga aagtttcatt gccagacggg
     5101 actcctgcaa tcgtcaaggg attgaaacct atagaagaca ttgctgatga actgcgcggg
     5161 gccgactatc tggtatggcg caatgggagg ggagcagtcc ggttgctcgg tcgtgagaac
     5221 aatctgatgt tgctcgaata tgccggggag cgaatgctct ctcacatcgt tgccgagcac
     5281 ggcgactacc aggcgaccga aattgcagcg gaactaatgg cgaagctgta tgccgcatct
     5341 gaggaacccc tgccttctgc ccttctcccg atccgggatc gctttgcagc tttgtttcag
     5401 cgggcgcgcg atgatcaaaa cgcaggttgt caaactgact acgtccacgc ggcgattata
     5461 gccgatcaaa tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga tctgcatcat
     5521 gaaaacatca tgttctccag tcgcggctgg ctggtgatag atcccgtcgg tctggtcggt
     5581 gaagtgggct ttggcgccgc caatatgttc tacgatccgg ctgacagaga cgacctttgt
     5641 ctcgatccta gacgcattgc acagatggcg gacgcattct ctcgtgcgct ggacgtcgat
     5701 ccgcgtcgcc tgctcgacca ggcgtacgct tatgggtgcc tttccgcagc ttggaacgcg
     5761 gatggagaag aggagcaacg cgatctagct atcgcggccg cgatcaagca ggtgcgacag
     5821 acgtcatact agatatcaag cgacttctcc tatcccctgg gaacacatca atctcaccgg
     5881 agaatatcgc tggccaaagc cttagcgtag gattccgccc cttcccgcaa acgacccctg
     5941 gaagccccgt agcgacgcgg agaggggcga gacaagccaa gggcgcaggc tcgatgcgca
     6001 gcacgacata gccggttctc gcaaggacga gaatttccct gcggtgcccc tcaagtgtca
     6061 atgaaagttt ccaacgcgag ccattcgcga gagccttgag tccacgctag atctatctca
     6121 tctgcgcaag gcagaacgtg aagacggccg ccctggacct cgcccgcgag cgccaggcgc
     6181 acgaggccgg cgcgcggacc cgcgccacgg cccacgagcg gacgccgcag caggagcgcc
     6241 agaaggccgc cagagaggcc gagcgcggcc gtgaggcttg gacgctaggg cagggcatga
     6301 aaaagcccgt agcgggctgc tacgggcgtc tgacgcggtg gaaaggggga ggggatgttg
     6361 tctacatggc tctgctgtag tgagtgggtt gcgctccggc agcggtcctg atcaatcgtc
     6421 accctttctc ggtccttcaa cgttcctgac aacgagcctc cttttcgcca atccatcgac
     6481 aatcaccgcg agtccctgct cgaacgctgc gtccggaccg gcttcgtcga aggcgtctat
     6541 cgcggcccgc aacagcggcg agagcggagc ctgttcaacg gtgccgccgc gctcgccggc
     6601 atcgctgtcg ccggcctgct cctcaagcac ggccccaaca gtgaagtagc tgattgtcat
     6661 cagcgcattg acggcgtccc cggccgaaaa acccgcctcg cagaggaagc gaagctgcgc
     6721 gtcggccgtt tccatctgcg gtgcgcccgg tcgcgtgccg gcatggatgc gcgcgccatc
     6781 gcggtaggcg agcagcgcct gcctgaagct gcgggcattc ccgatcagaa atgagcgcca
     6841 gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc gccagcatgg cttcggccag
     6901 tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa agcgccggct gctgaacccc
     6961 caaccgttcc gccagtttgc gtgtcgtcag accgtctacg ccgacctcgt tcaacaggtc
     7021 cagggcggca cggatcactg tattcggctg caactttgtc atgcttgaca ctttatcact
     7081 gataaacata atatgtccac caacttatca gtgataaaga atccgcgcgt tcaatcggac
     7141 cagcggaggc tggtccggag gccagacgtg aaacccaaca gacccctgat cgtaattctg
     7201 agcactgtcg cgctcgacgc tgtcggcatc ggcctgatta tgccggtgct gccgggcctc
     7261 ctgcgcgatc tggttcactc gaacgacgtc accgcccact atggcattct gctggcgctg
     7321 tatgcgttga tgcaatttgc ctgcgcacct gtgctgggcg cgctgtcgga tcgtttcggg
     7381 cggcggccgg tcttgctcgt ctcgctggcc ggcgctgctg tcgactacgc catcatggcg
     7441 acggcgcctt tcctttgggt tctctatatc gggcggatcg tggccggcat caccggggcg
     7501 actggggcgg tagccggcgc ttatattgcc gatatcactg atggcgatga gcgcgcgcgg
     7561 cacttcggct tcatgagcgc ctgtttcggg ttcgggatgg tcgcgggacc tgtgctcggt
     7621 gggctgatgg gcggtttctc cccccacgct ccgttcttcg ccgcggcagc cttgaacggc
     7681 ctcaatttcc tgacgggctg tttccttttg ccggagtcgc acaaaggcga acgccggccg
     7741 ttacgccggg aggctctcaa cccgctcgct tcgttccggt gggcccgggg catgaccgtc
     7801 gtcgccgccc tgatggcggt cttcttcatc atgcaacttg tcggacaggt gccggccgcg
     7861 ctttgggtca ttttcggcga ggatcgcttt cactgggacg cgaccacgat cggcatttcg
     7921 cttgccgcat ttggcattct gcattcactc gcccaggcaa tgatcaccgg ccctgtagcc
     7981 gcccggctcg gcgaaaggcg ggcactcatg ctcggaatga ttgccgacgg cacaggctac
     8041 atcctgcttg ccttcgcgac acggggatgg atggcgttcc cgatcatggt cctgcttgct
     8101 tcgggtggca tcggaatgcc ggcgctgcaa gcaatgttgt ccaggcaggt ggatgaggaa
     8161 cgtcaggggc agctgcaagg ctcactggcg gcgctcacca gcctgacctc gatcgtcgga
     8221 cccctcctct tcacggcgat ctatgcggct tctataacaa cgtggaacgg gtgggcatgg
     8281 attgcaggcg ctgccctcta cttgctctgc ctgccggcgc tgcgtcgcgg gctttggaga
     8341 aattcttcaa attcccgttg cacatagccc ggcaattcct ttccctgctc tgccataagc
     8401 g
//
