LOCUS       NZ_OP038299             8375 bp    DNA     circular CON 07-JAN-2024
DEFINITION  Shigella sonnei strain 202108535 plasmid p202108535-7, complete
            sequence.
ACCESSION   NZ_OP038299
VERSION     NZ_OP038299.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Recent and rapid emergence of extensively drug-resistant Shigella
            sonnei
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8375)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2022) Enteric Bacterial Pathogens Unit, Institut
            Pasteur, 28 rue du Dr Roux, Paris 75724, France
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Sequencing Technology             :: MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/07/2024 04:05:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP038299.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8375
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="202108535"
                     /isolation_source="human"
                     /db_xref="taxon:624"
                     /plasmid="p202108535-7"
     gene            27..269
                     /locus_tag="OVP26_RS00005"
                     /old_locus_tag="p202108535-7__00001"
     CDS             27..269
                     /locus_tag="OVP26_RS00005"
                     /old_locus_tag="p202108535-7__00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(301..951)
                     /gene="tetR(A)"
                     /locus_tag="OVP26_RS00010"
                     /old_locus_tag="p202108535-7__00002"
     CDS             complement(301..951)
                     /gene="tetR(A)"
                     /locus_tag="OVP26_RS00010"
                     /old_locus_tag="p202108535-7__00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            1057..2256
                     /gene="tet(A)"
                     /locus_tag="OVP26_RS00015"
                     /old_locus_tag="p202108535-7__00003"
     ARG             1058..2253
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<2223..2411)
                     /locus_tag="OVP26_RS00020"
                     /old_locus_tag="p202108535-7__00004"
                     /pseudo=""
     CDS             complement(<2223..2411)
                     /locus_tag="OVP26_RS00020"
                     /old_locus_tag="p202108535-7__00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(2455..2877)
                     /locus_tag="OVP26_RS00025"
                     /old_locus_tag="p202108535-7__00005"
     CDS             complement(2455..2877)
                     /locus_tag="OVP26_RS00025"
                     /old_locus_tag="p202108535-7__00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            3062..3502
                     /locus_tag="OVP26_RS00030"
                     /old_locus_tag="p202108535-7__00006"
     CDS             3062..3502
                     /locus_tag="OVP26_RS00030"
                     /old_locus_tag="p202108535-7__00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(4247..4396)
                     /locus_tag="OVP26_RS00035"
                     /old_locus_tag="p202108535-7__00007"
     CDS             complement(4247..4396)
                     /locus_tag="OVP26_RS00035"
                     /old_locus_tag="p202108535-7__00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            4967..5125
                     /locus_tag="OVP26_RS00055"
     CDS             4967..5125
                     /locus_tag="OVP26_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            5585..6400
                     /gene="sul2"
                     /locus_tag="OVP26_RS00040"
                     /old_locus_tag="p202108535-7__00008"
     ARG             5586..6397
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6461..7264
                     /gene="aph(3'')-Ib"
                     /locus_tag="OVP26_RS00045"
                     /old_locus_tag="p202108535-7__00009"
     ARG             6462..7261
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            7264..8100
                     /locus_tag="OVP26_RS00050"
                     /old_locus_tag="p202108535-7__00010"
     CDS             7264..8100
                     /locus_tag="OVP26_RS00050"
                     /old_locus_tag="p202108535-7__00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     ARG             7265..8097
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(4432..4605)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          4529..5134
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tctatctcat ctgcgcaagg cagaacgtga agacggccgc cctggacctc gcccgcgagc
       61 gccaggcgca cgaggccggc gcgcggaccc gcgccacggc ccacgagcgg acgccgcagc
      121 aggagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
      181 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
      241 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
      301 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
      361 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
      421 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
      481 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
      541 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
      601 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
      661 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
      721 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
      781 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
      841 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
      901 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
      961 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     1021 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacag acccctgatc
     1081 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     1141 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     1201 ctggcgctgt atgcgttgat gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     1261 cgtttcgggc ggcggccggt cttgctcgtc tcgctggccg gcgctgctgt cgactacgcc
     1321 atcatggcga cggcgccttt cctttgggtt ctctatatcg ggcggatcgt ggccggcatc
     1381 accggggcga ctggggcggt agccggcgct tatattgccg atatcactga tggcgatgag
     1441 cgcgcgcggc acttcggctt catgagcgcc tgtttcgggt tcgggatggt cgcgggacct
     1501 gtgctcggtg ggctgatggg cggtttctcc ccccacgctc cgttcttcgc cgcggcagcc
     1561 ttgaacggcc tcaatttcct gacgggctgt ttccttttgc cggagtcgca caaaggcgaa
     1621 cgccggccgt tacgccggga ggctctcaac ccgctcgctt cgttccggtg ggcccggggc
     1681 atgaccgtcg tcgccgccct gatggcggtc ttcttcatca tgcaacttgt cggacaggtg
     1741 ccggccgcgc tttgggtcat tttcggcgag gatcgctttc actgggacgc gaccacgatc
     1801 ggcatttcgc ttgccgcatt tggcattctg cattcactcg cccaggcaat gatcaccggc
     1861 cctgtagccg cccggctcgg cgaaaggcgg gcactcatgc tcggaatgat tgccgacggc
     1921 acaggctaca tcctgcttgc cttcgcgaca cggggatgga tggcgttccc gatcatggtc
     1981 ctgcttgctt cgggtggcat cggaatgccg gcgctgcaag caatgttgtc caggcaggtg
     2041 gatgaggaac gtcaggggca gctgcaaggc tcactggcgg cgctcaccag cctgacctcg
     2101 atcgtcggac ccctcctctt cacggcgatc tatgcggctt ctataacaac gtggaacggg
     2161 tgggcatgga ttgcaggcgc tgccctctac ttgctctgcc tgccggcgct gcgtcgcggg
     2221 ctttggagaa attcttcaaa ttcccgttgc acatagcccg gcaattcctt tccctgctct
     2281 gccataagcg cagcgaatgc cgggtaatac tcgtcaacga tctgatagag aagggtttgc
     2341 tcgggtcggt ggctctggta acgaccagta tcccgatccc ggctggccgt cctggccgcc
     2401 acatgaggca tgttccgcgt ccttgcaata ctgtgtttac atacagtcta tcgcttagcg
     2461 gaaagttctt ttaccctcag ccgaaatgcc tgccgttgct agacattgcc agccagtgcc
     2521 cgtcactccc actcttacat tgttcgcagt atctaggtac atgttattgc gtgcaccttc
     2581 atgatgtata atgttattaa tgacagcaga aagatcgaca gaattaatat tcttcgtacc
     2641 tgggacagtt atcgggtgtc ggccagtgat gagaccaaag gatgcagtat aagatgaacc
     2701 actactatta tcatggccac cattaccacc accgttaaaa ctacctcccg aacttccaga
     2761 gccgctatga ccatcattgt aggggtcatt tggtcccatg tgaccaaaac caccgacagt
     2821 accgctattt aactctgtac tcataaaatt atcctctttg acttttaaaa acagcaaaaa
     2881 ctgtatttat aacaatgtgt ttatatacag tattcgaaat actacgcccg tcaaaaccac
     2941 accgtcaaca ggttttttgt gtatttttca cgcctcacgg gcagcctcag accttttgtg
     3001 ttaaaacagt aatatgttct gctgttacaa tacggtacac acaggagaca ctgtcacttt
     3061 tatggcatgg cacacagttc aagagacact gaaattaaca ggaaagtcgc gctcgcaatt
     3121 atatcgtgac atggctaaag gccttgtcag ctatcgcaca ggaaaaaatg atcgccggga
     3181 gttcgagacc agtgaactga ttcgggccta tggcgagctg cgcacttctg agacaccaaa
     3241 aaggcacaag gagggacact ccaaatctgt cgttagccag gcggaaaatg ctcagctaga
     3301 cgtcattcag cagaaactgg acgctctgca gcagactgtt accctaatgc ttgaagacaa
     3361 gatggcgcgg gaagcagaaa accgcctgcg aaatgaggag agggaacaac tacaggccga
     3421 aattagccgt ctggctgacg cgctggcaca ggaaaaaaag cggggattct ggtcgcggct
     3481 gtttcgctcc ggttcagagt aacagtgcag gtgactctga tttaccataa catctgttat
     3541 cagcaccgcc tgagaggccc tcagggcgtt tctgacggga aaccggtaaa acaggcaaca
     3601 aaccacccga aaacccgcca gaaacgcgca ggctctgttt ttacggcatg catgagtatg
     3661 cgcacaagtg catagcgcgg aaattcacac cattcagccg gggtaagtcc tgtttccggc
     3721 gttcttccca tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc gtgaagcgtt
     3781 gccggcgcag ccggaaaacc cgctgtcagc acgttcagga agggggtgat gtacaaatgg
     3841 cacagaggat gaaagtgacg tatgagctga aggaatgatg taaaagtgat gtataagtga
     3901 tgtaaaagtg atgtatgaac tgaaggagcg atgtaattct gtagtcagga agaataaccc
     3961 gttcatgcag gcatgaacga tacagtgagg aacgcttttt ttgtcacgat ctccccttaa
     4021 cctgcgtaaa tcacccgcca tggttgatta tttttcaacc tcaaaatggt catacaccta
     4081 acgcagccgc gattttcatc gcaactgcat gaattaaaag aaaaaaaagg aatatttcgt
     4141 tttcacgagt aatacccggg tattaaaaat ccactgtaat tacatgaaaa taaagaaata
     4201 aaaggtaata ccggagtatt accacgtacg cgggcaagcg aaaaagctat aacgcttccc
     4261 ggccgacagg cgacccaaaa tccacagggg catgcaggtt ctcttcagta acctgcgcca
     4321 ttaaatcaaa aaggtcatat tcgccgggaa gctgcgtatc agtcgaatcc agttgttcat
     4381 catattcaga tggcatgggg tgatatccag tagctgagag gttgttagcg ggtttcgggg
     4441 cgcagccctg aaccagtcat gtagcgctag cggagtgtat actggcttac tatgttgcca
     4501 cgattgcgaa aatcagtgaa gtgcttcatg tggcaggaga aaaaaggcag caccggcgcg
     4561 tcagcagaat atgcgataca ggagatattc cgcttcctcg ctcactgact cgctacgctc
     4621 ggtcgttcgg ctgcggcgag cggtaccggc ttacggacgg ggacggaaat ttcctggaag
     4681 ataccaggaa gacacttagc aaagaagaga taaggccacg gcgaagccgt ttttccatag
     4741 gctccgcccc cctgacaagc atcgcaaaat ctgacgctca aatcagtggt ggcgaaaccc
     4801 gacaggacta taaagatccc aggcgtttcc ccctggtagc tccctcgtgc gctctcctgt
     4861 tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcggtttc tcattccacg
     4921 cctgacactc agttccgtga aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     4981 cgttcagtcc gaccgctgcg ccttgtccgg taactatcaa cttgagtcca acccggaaag
     5041 acacgacaaa acgccactgg cagcagccat tagtaacagg agtgcacgag aagatagatt
     5101 aagaggtctt gaagtagagg cctgactgcg gctacactga aagaacagtt ttggtaatgg
     5161 cggtctcgtg aaccctgtta ccgggttaag cagttcccca actgacttaa ccttcgaaca
     5221 aaccgcctcc ccaggcggtt ttttcgttta cagagcaaga gattacgatt cattgaaaag
     5281 gatctcaaga agatcctttg tagattacta tcaattaatc ttccgaacat gacaccagtc
     5341 actgttgatt acggttaaac cgctccgtaa caggtttccc tcgtattggt gacttgcagc
     5401 accaaagaag caaaaaagat aaatatatct acttattgag agatgatata tttatttcca
     5461 aaatgctaca gatacatcat catgaaacgg atcaccgcaa acaggttact cgcctgggga
     5521 ttccctttcg acccgagcat ccgtatgaga ctcatgctcg attattatta ttatagaagc
     5581 ccccatgaat aaatcgctca tcattttcgg catcgtcaac ataacctcgg acagtttctc
     5641 cgatggaggc cggtatctgg cgccagacgc agccattgcg caggcgcgta agctgatggc
     5701 cgagggggca gatgtgatcg acctcggtcc ggcatccagc aatcccgacg ccgcgcctgt
     5761 ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg gacgcgctca aggcagatgg
     5821 cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa gcctatgcct tgtcgcgtgg
     5881 tgtggcctat ctcaatgata ttcgcggttt tccagacgct gcgttctatc cgcaattggc
     5941 gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa gacgggcagg cagatcggcg
     6001 cgaggcaccc gctggcgaca tcatggatca cattgcggcg ttctttgacg cgcgcatcgc
     6061 ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc cttgatcccg gcatggggtt
     6121 ttttctgggg gctgctcccg aaacctcgct ctcggtgctg gcgcggttcg atgaattgcg
     6181 gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc aaatcctttc tgcgcgcgct
     6241 cacaggccgt ggtccggggg atgtcggggc cgcgacactc gctgcagagc ttgccgccgc
     6301 cgcaggtgga gctgacttca tccgcacaca cgagccgcgc cccttgcgcg acgggctggc
     6361 ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa ctgcacattc gggatatttc
     6421 tctatattcg cgcttcatca gaaaactgaa ggaacctcca ttgaatcgaa ctaatatttt
     6481 ttttggtgaa tcgcattctg actggttgcc tgtcagaggc ggagaatctg gtgattttgt
     6541 ttttcgacgt ggtgacgggc atgccttcgc gaaaatcgca cctgcttccc gccgcggtga
     6601 gctcgctgga gagcgtgacc gcctcatttg gctcaaaggt cgaggtgtgg cttgccccga
     6661 ggtcatcaac tggcaggagg aacaggaggg tgcatgcttg gtgataacgg caattccggg
     6721 agtaccggcg gctgatctgt ctggagcgga tttgctcaaa gcgtggccgt caatggggca
     6781 gcaacttggc gctgttcaca gcctatcggt tgatcaatgt cagtttgagc gcaggctgtc
     6841 gcgaatgttc ggacgcgccg ttgatgtggt gtcccgcaat gccgtcaatc ccgacttctt
     6901 accggacgag gacaagagta cgccgcagct cgatcttttg gctcgtgtcg aacgagagct
     6961 accggtgcgg ctcgaccaag agcgcaccga tatggttgtt tgccatggtg atccctgcat
     7021 gccgaacttc atggtggacc ctaaaactct tcaatgcacg ggtctgatcg accttgggcg
     7081 gctcggaaca gcagatcgct atgccgattt ggcactcatg attgctaacg ccgaagagaa
     7141 ctgggcagcg ccagatgaag cagagcgcgc cttcgctgtc ctattcaatg tattggggat
     7201 cgaagccccc gaccgcgaac gccttgcctt ctatctgcga ttggaccctc tgacttgggg
     7261 ttgatgttca tgccgcctgt ttttcctgct cattggcacg tttcgcaacc tgttctcatt
     7321 gcggacacct tttccagcct cgtttggaaa gtttcattgc cagacgggac tcctgcaatc
     7381 gtcaagggat tgaaacctat agaagacatt gctgatgaac tgcgcggggc cgactatctg
     7441 gtatggcgca atgggagggg agcagtccgg ttgctcggtc gtgagaacaa tctgatgttg
     7501 ctcgaatatg ccggggagcg aatgctctct cacatcgttg ccgagcacgg cgactaccag
     7561 gcgaccgaaa ttgcagcgga actaatggcg aagctgtatg ccgcatctga ggaacccctg
     7621 ccttctgccc ttctcccgat ccgggatcgc tttgcagctt tgtttcagcg ggcgcgcgat
     7681 gatcaaaacg caggttgtca aactgactac gtccacgcgg cgattatagc cgatcaaatg
     7741 atgagcaatg cctcggaact gcgtgggcta catggcgatc tgcatcatga aaacatcatg
     7801 ttctccagtc gcggctggct ggtgatagat cccgtcggtc tggtcggtga agtgggcttt
     7861 ggcgccgcca atatgttcta cgatccggct gacagagacg acctttgtct cgatcctaga
     7921 cgcattgcac agatggcgga cgcattctct cgtgcgctgg acgtcgatcc gcgtcgcctg
     7981 ctcgaccagg cgtacgctta tgggtgcctt tccgcagctt ggaacgcgga tggagaagag
     8041 gagcaacgcg atctagctat cgcggccgcg atcaagcagg tgcgacagac gtcatactag
     8101 atatcaagcg acttctccta tcccctggga acacatcaat ctcaccggag aatatcgctg
     8161 gccaaagcct tagcgtagga ttccgcccct tcccgcaaac gacccctgga agccccgtag
     8221 cgacgcggag aggggcgaga caagccaagg gcgcaggctc gatgcgcagc acgacatagc
     8281 cggttctcgc aaggacgaga atttccctgc ggtgcccctc aagtgtcaat gaaagtttcc
     8341 aacgcgagcc attcgcgaga gccttgagtc cacgc
//
