LOCUS       NZ_OP038296             8379 bp    DNA     circular CON 07-JAN-2024
DEFINITION  Shigella sonnei strain 202106367 plasmid p202106367-6, complete
            sequence.
ACCESSION   NZ_OP038296
VERSION     NZ_OP038296.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Recent and rapid emergence of extensively drug-resistant Shigella
            sonnei
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2022) Enteric Bacterial Pathogens Unit, Institut
            Pasteur, 28 rue du Dr Roux, Paris 75724, France
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Sequencing Technology             :: MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/07/2024 04:23:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP038296.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="202106367"
                     /isolation_source="human"
                     /db_xref="taxon:624"
                     /plasmid="p202106367-6"
     gene            complement(join(8150..8379,1..211))
                     /locus_tag="OVO85_RS00005"
     CDS             complement(join(8150..8379,1..211))
                     /locus_tag="OVO85_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            396..818
                     /locus_tag="OVO85_RS00010"
                     /old_locus_tag="p202106367-6__00001"
     CDS             396..818
                     /locus_tag="OVO85_RS00010"
                     /old_locus_tag="p202106367-6__00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            862..>1050
                     /locus_tag="OVO85_RS00015"
                     /old_locus_tag="p202106367-6__00002"
                     /pseudo=""
     CDS             862..>1050
                     /locus_tag="OVO85_RS00015"
                     /old_locus_tag="p202106367-6__00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(1017..2216)
                     /gene="tet(A)"
                     /locus_tag="OVO85_RS00020"
                     /old_locus_tag="p202106367-6__00003"
     ARG             complement(1021..2216)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2322..2972
                     /gene="tetR(A)"
                     /locus_tag="OVO85_RS00025"
                     /old_locus_tag="p202106367-6__00004"
     CDS             2322..2972
                     /gene="tetR(A)"
                     /locus_tag="OVO85_RS00025"
                     /old_locus_tag="p202106367-6__00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            complement(3004..3246)
                     /locus_tag="OVO85_RS00030"
                     /old_locus_tag="p202106367-6__00005"
     CDS             complement(3004..3246)
                     /locus_tag="OVO85_RS00030"
                     /old_locus_tag="p202106367-6__00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(3552..4388)
                     /locus_tag="OVO85_RS00035"
                     /old_locus_tag="p202106367-6__00006"
     CDS             complement(3552..4388)
                     /locus_tag="OVO85_RS00035"
                     /old_locus_tag="p202106367-6__00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(4388..5191)
                     /gene="aph(3'')-Ib"
                     /locus_tag="OVO85_RS00040"
                     /old_locus_tag="p202106367-6__00007"
     ARG             complement(4392..5191)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            complement(5252..6067)
                     /gene="sul2"
                     /locus_tag="OVO85_RS00045"
                     /old_locus_tag="p202106367-6__00008"
     ARG             complement(5256..6067)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(6527..6685)
                     /locus_tag="OVO85_RS00055"
     CDS             complement(6527..6685)
                     /locus_tag="OVO85_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            7256..7405
                     /locus_tag="OVO85_RS00050"
                     /old_locus_tag="p202106367-6__00009"
     CDS             7256..7405
                     /locus_tag="OVO85_RS00050"
                     /old_locus_tag="p202106367-6__00009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     ARG             complement(3556..4388)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          7048..7221
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(6519..7124)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cgacagattt ggagtgtccc tccttgtgcc tttttggtgt ctcagaagtg cgcagctcgc
       61 cataggcccg aatcagttca ctggtctcga actcccggcg atcatttttt cctgtgcgat
      121 agctgacaag gcctttagcc atgtcacgat ataattgcga gcgcgacttt cctgttaatt
      181 tcagtgtctc ttgaactgtg tgccatgcca taaaagtgac agtgtctcct gtgtgtaccg
      241 tattgtaaca gcagaacata ttactgtttt aacacaaaag gtctgaggct gcccgtgagg
      301 cgtgaaaaat acacaaaaaa cctgttgacg gtgtggtttt gacgggcgta gtatttcgaa
      361 tactgtatat aaacacattg ttataaatac agtttttgct gtttttaaaa gtcaaagagg
      421 ataattttat gagtacagag ttaaatagcg gtactgtcgg tggttttggt cacatgggac
      481 caaatgaccc ctacaatgat ggtcatagcg gctctggaag ttcgggaggt agttttaacg
      541 gtggtggtaa tggtggccat gataatagta gtggttcatc ttatactgca tcctttggtc
      601 tcatcactgg ccgacacccg ataactgtcc caggtacgaa gaatattaat tctgtcgatc
      661 tttctgctgt cattaataac attatacatc atgaaggtgc acgcaataac atgtacctag
      721 atactgcgaa caatgtaaga gtgggagtga cgggcactgg ctggcaatgt ctagcaacgg
      781 caggcatttc ggctgagggt aaaagaactt tccgctaagc gatagactgt atgtaaacac
      841 agtattgcaa ggacgcggaa catgcctcat gtggcggcca ggacggccag ccgggatcgg
      901 gatactggtc gttaccagag ccaccgaccc gagcaaaccc ttctctatca gatcgttgac
      961 gagtattacc cggcattcgc tgcgcttatg gcagagcagg gaaaggaatt gccgggctat
     1021 gtgcaacggg aatttgaaga atttctccaa agcccgcgac gcagcgccgg caggcagagc
     1081 aagtagaggg cagcgcctgc aatccatgcc cacccgttcc acgttgttat agaagccgca
     1141 tagatcgccg tgaagaggag gggtccgacg atcgaggtca ggctggtgag cgccgccagt
     1201 gagccttgca gctgcccctg acgttcctca tccacctgcc tggacaacat tgcttgcagc
     1261 gccggcattc cgatgccacc cgaagcaagc aggaccatga tcgggaacgc catccatccc
     1321 cgtgtcgcga aggcaagcag gatgtagcct gtgccgtcgg caatcattcc gagcatgagt
     1381 gcccgccttt cgccgagccg ggcggctaca gggccggtga tcattgcctg ggcgagtgaa
     1441 tgcagaatgc caaatgcggc aagcgaaatg ccgatcgtgg tcgcgtccca gtgaaagcga
     1501 tcctcgccga aaatgaccca aagcgcggcc ggcacctgtc cgacaagttg catgatgaag
     1561 aagaccgcca tcagggcggc gacgacggtc atgccccggg cccaccggaa cgaagcgagc
     1621 gggttgagag cctcccggcg taacggccgg cgttcgcctt tgtgcgactc cggcaaaagg
     1681 aaacagcccg tcaggaaatt gaggccgttc aaggctgccg cggcgaagaa cggagcgtgg
     1741 ggggagaaac cgcccatcag cccaccgagc acaggtcccg cgaccatccc gaacccgaaa
     1801 caggcgctca tgaagccgaa gtgccgcgcg cgctcatcgc catcagtgat atcggcaata
     1861 taagcgccgg ctaccgcccc agtcgccccg gtgatgccgg ccacgatccg cccgatatag
     1921 agaacccaaa ggaaaggcgc cgtcgccatg atggcgtagt cgacagcagc gccggccagc
     1981 gagacgagca agaccggccg ccgcccgaaa cgatccgaca gcgcgcccag cacaggtgcg
     2041 caggcaaatt gcatcaacgc atacagcgcc agcagaatgc catagtgggc ggtgacgtcg
     2101 ttcgagtgaa ccagatcgcg caggaggccc ggcagcaccg gcataatcag gccgatgccg
     2161 acagcgtcga gcgcgacagt gctcagaatt acgatcaggg gtctgttggg tttcacgtct
     2221 ggcctccgga ccagcctccg ctggtccgat tgaacgcgcg gattctttat cactgataag
     2281 ttggtggaca tattatgttt atcagtgata aagtgtcaag catgacaaag ttgcagccga
     2341 atacagtgat ccgtgccgcc ctggacctgt tgaacgaggt cggcgtagac ggtctgacga
     2401 cacgcaaact ggcggaacgg ttgggggttc agcagccggc gctttactgg cacttcagga
     2461 acaagcgggc gctgctcgac gcactggccg aagccatgct ggcggagaat catacgcatt
     2521 cggtgccgag agccgacgac gactggcgct catttctgat cgggaatgcc cgcagcttca
     2581 ggcaggcgct gctcgcctac cgcgatggcg cgcgcatcca tgccggcacg cgaccgggcg
     2641 caccgcagat ggaaacggcc gacgcgcagc ttcgcttcct ctgcgaggcg ggtttttcgg
     2701 ccggggacgc cgtcaatgcg ctgatgacaa tcagctactt cactgttggg gccgtgcttg
     2761 aggagcaggc cggcgacagc gatgccggcg agcgcggcgg caccgttgaa caggctccgc
     2821 tctcgccgct gttgcgggcc gcgatagacg ccttcgacga agccggtccg gacgcagcgt
     2881 tcgagcaggg actcgcggtg attgtcgatg gattggcgaa aaggaggctc gttgtcagga
     2941 acgttgaagg accgagaaag ggtgacgatt gatcaggacc gctgccggag cgcaacccac
     3001 tcactacagc agagccatgt agacaacatc ccctccccct ttccaccgcg tcagacgccc
     3061 gtagcagccc gctacgggct ttttcatgcc ctgccctagc gtccaagcct cacggccgcg
     3121 ctcggcctct ctggcggcct tctggcgctc ctgctgcggc gtccgctcgt gggccgtggc
     3181 gcgggtccgc gcgccggcct cgtgcgcctg gcgctcgcgg gcgaggtcca gggcggccgt
     3241 cttcacgttc tgccttgcgc agatgagata gatctagcgt ggactcaagg ctctcgcgaa
     3301 tggctcgcgt tggaaacttt cattgacact tgaggggcac cgcagggaaa ttctcgtcct
     3361 tgcgagaacc ggctatgtcg tgctgcgcat cgagcctgcg cccttggctt gtctcgcccc
     3421 tctccgcgtc gctacggggc ttccaggggt cgtttgcggg aaggggcgga atcctacgct
     3481 aaggctttgg ccagcgatat tctccggtga gattgatgtg ttcccagggg ataggagaag
     3541 tcgcttgata tctagtatga cgtctgtcgc acctgcttga tcgcggccgc gatagctaga
     3601 tcgcgttgct cctcttctcc atccgcgttc caagctgcgg aaaggcaccc ataagcgtac
     3661 gcctggtcga gcaggcgacg cggatcgacg tccagcgcac gagagaatgc gtccgccatc
     3721 tgtgcaatgc gtctaggatc gagacaaagg tcgtctctgt cagccggatc gtagaacata
     3781 ttggcggcgc caaagcccac ttcaccgacc agaccgacgg gatctatcac cagccagccg
     3841 cgactggaga acatgatgtt ttcatgatgc agatcgccat gtagcccacg cagttccgag
     3901 gcattgctca tcatttgatc ggctataatc gccgcgtgga cgtagtcagt ttgacaacct
     3961 gcgttttgat catcgcgcgc ccgctgaaac aaagctgcaa agcgatcccg gatcgggaga
     4021 agggcagaag gcaggggttc ctcagatgcg gcatacagct tcgccattag ttccgctgca
     4081 atttcggtcg cctggtagtc gccgtgctcg gcaacgatgt gagagagcat tcgctccccg
     4141 gcatattcga gcaacatcag attgttctca cgaccgagca accggactgc tcccctccca
     4201 ttgcgccata ccagatagtc ggccccgcgc agttcatcag caatgtcttc tataggtttc
     4261 aatcccttga cgattgcagg agtcccgtct ggcaatgaaa ctttccaaac gaggctggaa
     4321 aaggtgtccg caatgagaac aggttgcgaa acgtgccaat gagcaggaaa aacaggcggc
     4381 atgaacatca accccaagtc agagggtcca atcgcagata gaaggcaagg cgttcgcggt
     4441 cgggggcttc gatccccaat acattgaata ggacagcgaa ggcgcgctct gcttcatctg
     4501 gcgctgccca gttctcttcg gcgttagcaa tcatgagtgc caaatcggca tagcgatctg
     4561 ctgttccgag ccgcccaagg tcgatcagac ccgtgcattg aagagtttta gggtccacca
     4621 tgaagttcgg catgcaggga tcaccatggc aaacaaccat atcggtgcgc tcttggtcga
     4681 gccgcaccgg tagctctcgt tcgacacgag ccaaaagatc gagctgcggc gtactcttgt
     4741 cctcgtccgg taagaagtcg ggattgacgg cattgcggga caccacatca acggcgcgtc
     4801 cgaacattcg cgacagcctg cgctcaaact gacattgatc aaccgatagg ctgtgaacag
     4861 cgccaagttg ctgccccatt gacggccacg ctttgagcaa atccgctcca gacagatcag
     4921 ccgccggtac tcccggaatt gccgttatca ccaagcatgc accctcctgt tcctcctgcc
     4981 agttgatgac ctcggggcaa gccacacctc gacctttgag ccaaatgagg cggtcacgct
     5041 ctccagcgag ctcaccgcgg cgggaagcag gtgcgatttt cgcgaaggca tgcccgtcac
     5101 cacgtcgaaa aacaaaatca ccagattctc cgcctctgac aggcaaccag tcagaatgcg
     5161 attcaccaaa aaaaatatta gttcgattca atggaggttc cttcagtttt ctgatgaagc
     5221 gcgaatatag agaaatatcc cgaatgtgca gttaacgaat tcttgcggtt tctttcagcg
     5281 ccgccaatac cgccagcccg tcgcgcaagg ggcgcggctc gtgtgtgcgg atgaagtcag
     5341 ctccacctgc ggcggcggca agctctgcag cgagtgtcgc ggccccgaca tcccccggac
     5401 cacggcctgt gagcgcgcgc agaaaggatt tgcgcgaaac agacagaagc accggcaaat
     5461 cgaagcgcag ccgcaattca tcgaaccgcg ccagcaccga gagcgaggtt tcgggagcag
     5521 cccccagaaa aaaccccatg ccgggatcaa ggacaaggcg gttgcgtttg ataccggcac
     5581 ccgtcagcgc cgcgatgcgc gcgtcaaaga acgccgcaat gtgatccatg atgtcgccag
     5641 cgggtgcctc gcgccgatct gcctgcccgt cttgcaccga atgcataacg acgagtttgg
     5701 cagatgattt cgccaattgc ggatagaacg cagcgtctgg aaaaccgcga atatcattga
     5761 gataggccac accacgcgac aaggcatagg cttgcgtcgc gggttgataa ctgtcgagcg
     5821 agacgggaat gccatctgcc ttgagcgcgt ccagcaccgg cgcgatacgc gcgatttctg
     5881 tgtcggacga aacaggcgcg gcgtcgggat tgctggatgc cggaccgagg tcgatcacat
     5941 ctgccccctc ggccatcagc ttacgcgcct gcgcaatggc tgcgtctggc gccagatacc
     6001 ggcctccatc ggagaaactg tccgaggtta tgttgacgat gccgaaaatg atgagcgatt
     6061 tattcatggg ggcttctata ataataataa tcgagcatga gtctcatacg gatgctcggg
     6121 tcgaaaggga atccccaggc gagtaacctg tttgcggtga tccgtttcat gatgatgtat
     6181 ctgtagcatt ttggaaataa atatatcatc tctcaataag tagatatatt tatctttttt
     6241 gcttctttgg tgctgcaagt caccaatacg agggaaacct gttacggagc ggtttaaccg
     6301 taatcaacag tgactggtgt catgttcgga agattaattg atagtaatct acaaaggatc
     6361 ttcttgagat ccttttcaat gaatcgtaat ctcttgctct gtaaacgaaa aaaccgcctg
     6421 gggaggcggt ttgttcgaag gttaagtcag ttggggaact gcttaacccg gtaacagggt
     6481 tcacgagacc gccattacca aaactgttct ttcagtgtag ccgcagtcag gcctctactt
     6541 caagacctct taatctatct tctcgtgcac tcctgttact aatggctgct gccagtggcg
     6601 ttttgtcgtg tctttccggg ttggactcaa gttgatagtt accggacaag gcgcagcggt
     6661 cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc ttcacggaac
     6721 tgagtgtcag gcgtggaatg agaaaccgcg gccataacag cggaatgaca ccggtaaacc
     6781 gaaaggcagg aacaggagag cgcacgaggg agctaccagg gggaaacgcc tgggatcttt
     6841 atagtcctgt cgggtttcgc caccactgat ttgagcgtca gattttgcga tgcttgtcag
     6901 gggggcggag cctatggaaa aacggcttcg ccgtggcctt atctcttctt tgctaagtgt
     6961 cttcctggta tcttccagga aatttccgtc cccgtccgta agccggtacc gctcgccgca
     7021 gccgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaatatctc ctgtatcgca
     7081 tattctgctg acgcgccggt gctgcctttt ttctcctgcc acatgaagca cttcactgat
     7141 tttcgcaatc gtggcaacat agtaagccag tatacactcc gctagcgcta catgactggt
     7201 tcagggctgc gccccgaaac ccgctaacaa cctctcagct actggatatc accccatgcc
     7261 atctgaatat gatgaacaac tggattcgac tgatacgcag cttcccggcg aatatgacct
     7321 ttttgattta atggcgcagg ttactgaaga gaacctgcat gcccctgtgg attttgggtc
     7381 gcctgtcggc cgggaagcgt tatagctttt tcgcttgccc gcgtacgtgg taatactccg
     7441 gtattacctt ttatttcttt attttcatgt aattacagtg gatttttaat acccgggtat
     7501 tactcgtgaa aacgaaatat tccttttttt tcttttaatt catgcagttg cgatgaaaat
     7561 cgcggctgcg ttaggtgtat gaccattttg aggttgaaaa ataatcaacc atggcgggtg
     7621 atttacgcag gttaagggga gatcgtgaca aaaaaagcgt tcctcactgt atcgttcatg
     7681 cctgcatgaa cgggttattc ttcctgacta cagaattaca tcgctccttc agttcataca
     7741 tcacttttac atcacttata catcactttt acatcattcc ttcagctcat acgtcacttt
     7801 catcctctgt gccatttgta catcaccccc ttcctgaacg tgctgacagc gggttttccg
     7861 gctgcgccgg caacgcttca cggccacgca ataaattgcg tccccgttca ccgttttcgg
     7921 atgggaagaa cgccggaaac aggacttacc ccggctgaat ggtgtgaatt tccgcgctat
     7981 gcacttgtgc gcatactcat gcatgccgta aaaacagagc ctgcgcgttt ctggcgggtt
     8041 ttcgggtggt ttgttgcctg ttttaccggt ttcccgtcag aaacgccctg agggcctctc
     8101 aggcggtgct gataacagat gttatggtaa atcagagtca cctgcactgt tactctgaac
     8161 cggagcgaaa cagccgcgac cagaatcccc gctttttttc ctgtgccagc gcgtcagcca
     8221 gacggctaat ttcggcctgt agttgttccc tctcctcatt tcgcaggcgg ttttctgctt
     8281 cccgcgccat cttgtcttca agcattaggg taacagtctg ctgcagagcg tccagtttct
     8341 gctgaatgac gtctagctga gcattttccg cctggctaa
//
