LOCUS       NZ_OP038294             8401 bp    DNA     circular CON 07-JAN-2024
DEFINITION  Shigella sonnei strain 202102843 plasmid p202102843-8, complete
            sequence.
ACCESSION   NZ_OP038294
VERSION     NZ_OP038294.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Recent and rapid emergence of extensively drug-resistant Shigella
            sonnei
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2022) Enteric Bacterial Pathogens Unit, Institut
            Pasteur, 28 rue du Dr Roux, Paris 75724, France
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Sequencing Technology             :: MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/07/2024 04:11:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10
            CDSs (total)                      :: 10
            Genes (coding)                    :: 9
            CDSs (with protein)               :: 9
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP038294.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="202102843"
                     /isolation_source="human"
                     /db_xref="taxon:624"
                     /plasmid="p202102843-8"
     gene            547..1362
                     /gene="sul2"
                     /locus_tag="OVO83_RS00005"
                     /old_locus_tag="p202102843-8__00001"
     ARG             548..1359
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1423..2226
                     /gene="aph(3'')-Ib"
                     /locus_tag="OVO83_RS00010"
                     /old_locus_tag="p202102843-8__00002"
     ARG             1424..2223
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            2226..3062
                     /locus_tag="OVO83_RS00015"
                     /old_locus_tag="p202102843-8__00003"
     CDS             2226..3062
                     /locus_tag="OVO83_RS00015"
                     /old_locus_tag="p202102843-8__00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            3368..3610
                     /locus_tag="OVO83_RS00020"
                     /old_locus_tag="p202102843-8__00004"
     CDS             3368..3610
                     /locus_tag="OVO83_RS00020"
                     /old_locus_tag="p202102843-8__00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(3642..4292)
                     /gene="tetR(A)"
                     /locus_tag="OVO83_RS00025"
                     /old_locus_tag="p202102843-8__00005"
     CDS             complement(3642..4292)
                     /gene="tetR(A)"
                     /locus_tag="OVO83_RS00025"
                     /old_locus_tag="p202102843-8__00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            4398..5597
                     /gene="tet(A)"
                     /locus_tag="OVO83_RS00030"
                     /old_locus_tag="p202102843-8__00006"
     ARG             4399..5594
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<5564..5752)
                     /locus_tag="OVO83_RS00035"
                     /old_locus_tag="p202102843-8__00007"
                     /pseudo=""
     CDS             complement(<5564..5752)
                     /locus_tag="OVO83_RS00035"
                     /old_locus_tag="p202102843-8__00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(5796..6218)
                     /locus_tag="OVO83_RS00040"
                     /old_locus_tag="p202102843-8__00008"
     CDS             complement(5796..6218)
                     /locus_tag="OVO83_RS00040"
                     /old_locus_tag="p202102843-8__00008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            6403..6843
                     /locus_tag="OVO83_RS00045"
                     /old_locus_tag="p202102843-8__00009"
     CDS             6403..6843
                     /locus_tag="OVO83_RS00045"
                     /old_locus_tag="p202102843-8__00009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(7610..7759)
                     /locus_tag="OVO83_RS00050"
                     /old_locus_tag="p202102843-8__00010"
     CDS             complement(7610..7759)
                     /locus_tag="OVO83_RS00050"
                     /old_locus_tag="p202102843-8__00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     ARG             2227..3059
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(7795..7968)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          7892..8401
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.634"
                     /coverage="84.0"
                     /evalue="0.0"
ORIGIN
        1 agacacgaca aaacgccact ggcagcagcc attagtaaca ggagtgcacg agaagataga
       61 ttaagaggtc ttgaagtaga ggcctgactg cggctacact gaaagaacag ttttggtaat
      121 ggcggtctcg tgaaccctgt taccgggtta agcagttccc caactgactt aaccttcgaa
      181 caaaccgcct ccccaggcgg ttttttcgtt tacagagcaa gagattacga ttcattgaaa
      241 aggatctcaa gaagatcctt tgtagattac tatcaattaa tcttccgaac atgacaccag
      301 tcactgttga ttacggttaa accgctccgt aacaggtttc cctcgtattg gtgacttgca
      361 gcaccaaaga agcaaaaaag ataaatatat ctacttattg agagatgata tatttatttc
      421 caaaatgcta cagatacatc atcatgaaac ggatcaccgc aaacaggtta ctcgcctggg
      481 gattcccttt cgacccgagc atccgtatga gactcatgct cgattattat tattatagaa
      541 gcccccatga ataaatcgct catcattttc ggcatcgtca acataacctc ggacagtttc
      601 tccgatggag gccggtatct ggcgccagac gcagccattg cgcaggcgcg taagctgatg
      661 gccgaggggg cagatgtgat cgacctcggt ccggcatcca gcaatcccga cgccgcgcct
      721 gtttcgtccg acacagaaat cgcgcgtatc gcgccggtgc tggacgcgct caaggcagat
      781 ggcattcccg tctcgctcga cagttatcaa cccgcgacgc aagcctatgc cttgtcgcgt
      841 ggtgtggcct atctcaatga tattcgcggt tttccagacg ctgcgttcta tccgcaattg
      901 gcgaaatcat ctgccaaact cgtcgttatg cattcggtgc aagacgggca ggcagatcgg
      961 cgcgaggcac ccgctggcga catcatggat cacattgcgg cgttctttga cgcgcgcatc
     1021 gcggcgctga cgggtgccgg tatcaaacgc aaccgccttg tccttgatcc cggcatgggg
     1081 ttttttctgg gggctgctcc cgaaacctcg ctctcggtgc tggcgcggtt cgatgaattg
     1141 cggctgcgct tcgatttgcc ggtgcttctg tctgtttcgc gcaaatcctt tctgcgcgcg
     1201 ctcacaggcc gtggtccggg ggatgtcggg gccgcgacac tcgctgcaga gcttgccgcc
     1261 gccgcaggtg gagctgactt catccgcaca cacgagccgc gccccttgcg cgacgggctg
     1321 gcggtattgg cggcgctgaa agaaaccgca agaattcgtt aactgcacat tcgggatatt
     1381 tctctatatt cgcgcttcat cagaaaactg aaggaacctc cattgaatcg aactaatatt
     1441 ttttttggtg aatcgcattc tgactggttg cctgtcagag gcggagaatc tggtgatttt
     1501 gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg cacctgcttc ccgccgcggt
     1561 gagctcgctg gagagcgtga ccgcctcatt tggctcaaag gtcgaggtgt ggcttgcccc
     1621 gaggtcatca actggcagga ggaacaggag ggtgcatgct tggtgataac ggcaattccg
     1681 ggagtaccgg cggctgatct gtctggagcg gatttgctca aagcgtggcc gtcaatgggg
     1741 cagcaacttg gcgctgttca cagcctatcg gttgatcaat gtcagtttga gcgcaggctg
     1801 tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca atgccgtcaa tcccgacttc
     1861 ttaccggacg aggacaagag tacgccgcag ctcgatcttt tggctcgtgt cgaacgagag
     1921 ctaccggtgc ggctcgacca agagcgcacc gatatggttg tttgccatgg tgatccctgc
     1981 atgccgaact tcatggtgga ccctaaaact cttcaatgca cgggtctgat cgaccttggg
     2041 cggctcggaa cagcagatcg ctatgccgat ttggcactca tgattgctaa cgccgaagag
     2101 aactgggcag cgccagatga agcagagcgc gccttcgctg tcctattcaa tgtattgggg
     2161 atcgaagccc ccgaccgcga acgccttgcc ttctatctgc gattggaccc tctgacttgg
     2221 ggttgatgtt catgccgcct gtttttcctg ctcattggca cgtttcgcaa cctgttctca
     2281 ttgcggacac cttttccagc ctcgtttgga aagtttcatt gccagacggg actcctgcaa
     2341 tcgtcaaggg attgaaacct atagaagaca ttgctgatga actgcgcggg gccgactatc
     2401 tggtatggcg caatgggagg ggagcagtcc ggttgctcgg tcgtgagaac aatctgatgt
     2461 tgctcgaata tgccggggag cgaatgctct ctcacatcgt tgccgagcac ggcgactacc
     2521 aggcgaccga aattgcagcg gaactaatgg cgaagctgta tgccgcatct gaggaacccc
     2581 tgccttctgc ccttctcccg atccgggatc gctttgcagc tttgtttcag cgggcgcgcg
     2641 atgatcaaaa cgcaggttgt caaactgact acgtccacgc ggcgattata gccgatcaaa
     2701 tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga tctgcatcat gaaaacatca
     2761 tgttctccag tcgcggctgg ctggtgatag atcccgtcgg tctggtcggt gaagtgggct
     2821 ttggcgccgc caatatgttc tacgatccgg ctgacagaga cgacctttgt ctcgatccta
     2881 gacgcattgc acagatggcg gacgcattct ctcgtgcgct ggacgtcgat ccgcgtcgcc
     2941 tgctcgacca ggcgtacgct tatgggtgcc tttccgcagc ttggaacgcg gatggagaag
     3001 aggagcaacg cgatctagct atcgcggccg cgatcaagca ggtgcgacag acgtcatact
     3061 agatatcaag cgacttctcc tatcccctgg gaacacatca atctcaccgg agaatatcgc
     3121 tggccaaagc cttagcgtag gattccgccc cttcccgcaa acgacccctg gaagccccgt
     3181 agcgacgcgg agaggggcga gacaagccaa gggcgcaggc tcgatgcgca gcacgacata
     3241 gccggttctc gcaaggacga gaatttccct gcggtgcccc tcaagtgtca atgaaagttt
     3301 ccaacgcgag ccattcgcga gagccttgag tccacgctag atctatctca tctgcgcaag
     3361 gcagaacgtg aagacggccg ccctggacct cgcccgcgag cgccaggcgc acgaggccgg
     3421 cgcgcggacc cgcgccacgg cccacgagcg gacgccgcag caggagcgcc agaaggccgc
     3481 cagagaggcc gagcgcggcc gtgaggcttg gacgctaggg cagggcatga aaaagcccgt
     3541 agcgggctgc tacgggcgtc tgacgcggtg gaaaggggga ggggatgttg tctacatggc
     3601 tctgctgtag tgagtgggtt gcgctccggc agcggtcctg atcaatcgtc accctttctc
     3661 ggtccttcaa cgttcctgac aacgagcctc cttttcgcca atccatcgac aatcaccgcg
     3721 agtccctgct cgaacgctgc gtccggaccg gcttcgtcga aggcgtctat cgcggcccgc
     3781 aacagcggcg agagcggagc ctgttcaacg gtgccgccgc gctcgccggc atcgctgtcg
     3841 ccggcctgct cctcaagcac ggccccaaca gtgaagtagc tgattgtcat cagcgcattg
     3901 acggcgtccc cggccgaaaa acccgcctcg cagaggaagc gaagctgcgc gtcggccgtt
     3961 tccatctgcg gtgcgcccgg tcgcgtgccg gcatggatgc gcgcgccatc gcggtaggcg
     4021 agcagcgcct gcctgaagct gcgggcattc ccgatcagaa atgagcgcca gtcgtcgtcg
     4081 gctctcggca ccgaatgcgt atgattctcc gccagcatgg cttcggccag tgcgtcgagc
     4141 agcgcccgct tgttcctgaa gtgccagtaa agcgccggct gctgaacccc caaccgttcc
     4201 gccagtttgc gtgtcgtcag accgtctacg ccgacctcgt tcaacaggtc cagggcggca
     4261 cggatcactg tattcggctg caactttgtc atgcttgaca ctttatcact gataaacata
     4321 atatgtccac caacttatca gtgataaaga atccgcgcgt tcaatcggac cagcggaggc
     4381 tggtccggag gccagacgtg aaacccaaca gacccctgat cgtaattctg agcactgtcg
     4441 cgctcgacgc tgtcggcatc ggcctgatta tgccggtgct gccgggcctc ctgcgcgatc
     4501 tggttcactc gaacgacgtc accgcccact atggcattct gctggcgctg tatgcgttga
     4561 tgcaatttgc ctgcgcacct gtgctgggcg cgctgtcgga tcgtttcggg cggcggccgg
     4621 tcttgctcgt ctcgctggcc ggcgctgctg tcgactacgc catcatggcg acggcgcctt
     4681 tcctttgggt tctctatatc gggcggatcg tggccggcat caccggggcg actggggcgg
     4741 tagccggcgc ttatattgcc gatatcactg atggcgatga gcgcgcgcgg cacttcggct
     4801 tcatgagcgc ctgtttcggg ttcgggatgg tcgcgggacc tgtgctcggt gggctgatgg
     4861 gcggtttctc cccccacgct ccgttcttcg ccgcggcagc cttgaacggc ctcaatttcc
     4921 tgacgggctg tttccttttg ccggagtcgc acaaaggcga acgccggccg ttacgccggg
     4981 aggctctcaa cccgctcgct tcgttccggt gggcccgggg catgaccgtc gtcgccgccc
     5041 tgatggcggt cttcttcatc atgcaacttg tcggacaggt gccggccgcg ctttgggtca
     5101 ttttcggcga ggatcgcttt cactgggacg cgaccacgat cggcatttcg cttgccgcat
     5161 ttggcattct gcattcactc gcccaggcaa tgatcaccgg ccctgtagcc gcccggctcg
     5221 gcgaaaggcg ggcactcatg ctcggaatga ttgccgacgg cacaggctac atcctgcttg
     5281 ccttcgcgac acggggatgg atggcgttcc cgatcatggt cctgcttgct tcgggtggca
     5341 tcggaatgcc ggcgctgcaa gcaatgttgt ccaggcaggt ggatgaggaa cgtcaggggc
     5401 agctgcaagg ctcactggcg gcgctcacca gcctgacctc gatcgtcgga cccctcctct
     5461 tcacggcgat ctatgcggct tctataacaa cgtggaacgg gtgggcatgg attgcaggcg
     5521 ctgccctcta cttgctctgc ctgccggcgc tgcgtcgcgg gctttggaga aattcttcaa
     5581 attcccgttg cacatagccc ggcaattcct ttccctgctc tgccataagc gcagcgaatg
     5641 ccgggtaata ctcgtcaacg atctgataga gaagggtttg ctcgggtcgg tggctctggt
     5701 aacgaccagt atcccgatcc cggctggccg tcctggccgc cacatgaggc atgttccgcg
     5761 tccttgcaat actgtgttta catacagtct atcgcttagc ggaaagttct tttaccctca
     5821 gccgaaatgc ctgccgttgc tagacattgc cagccagtgc ccgtcactcc cactcttaca
     5881 ttgttcgcag tatctaggta catgttattg cgtgcacctt catgatgtat aatgttatta
     5941 atgacagcag aaagatcgac agaattaata ttcttcgtac ctgggacagt tatcgggtgt
     6001 cggccagtga tgagaccaaa ggatgcagta taagatgaac cactactatt atcatggcca
     6061 ccattaccac caccgttaaa actacctccc gaacttccag agccgctatg accatcattg
     6121 taggggtcat ttggtcccat gtgaccaaaa ccaccgacag taccgctatt taactctgta
     6181 ctcataaaat tatcctcttt gacttttaaa aacagcaaaa actgtattta taacaatgtg
     6241 tttatataca gtattcgaaa tactacgccc gtcaaaacca caccgtcaac aggttttttg
     6301 tgtatttttc acgcctcacg ggcagcctca gaccttttgt gttaaaacag taatatgttc
     6361 tgctgttaca atacggtaca cacaggagac actgtcactt ttatggcatg gcacacagtt
     6421 caagagacac tgaaattaac aggaaagtcg cgctcgcaat tatatcgtga catggctaaa
     6481 ggccttgtca gctatcgcac aggaaaaaat gatcgccggg agttcgagac cagtgaactg
     6541 attcgggcct atggcgagct gcgcacttct gagacaccaa aaaggcacaa ggagggacac
     6601 tccaaatctg tcgttagcca ggcggaaaat gctcagctag acgtcattca gcagaaactg
     6661 gacgctctgc agcagactgt taccctaatg cttgaagaca agatggcgcg ggaagcagaa
     6721 aaccgcctgc gaaatgagga gagggaacaa ctacaggccg aaattagccg tctggctgac
     6781 gcgctggcac aggaaaaaaa gcggggattc tggtcgcggc tgtttcgctc cggttcagag
     6841 taacagtgca ggtgactctg atttaccata acatctgtta tcagcaccgc ctgagaggcc
     6901 ctcagggcgt ttctgacggg aaaccggtaa aacaggcaac aaaccacccg aaaacccgcc
     6961 agaaacgcgc aggctctgtt tttacggcat gcatgagtat gcgcacaagt gcatagcgcg
     7021 gaaattcaca ccattcagcc ggggtaagtc ctgtttccgg cgttcttccc atccgaaaac
     7081 ggtgaacggg gacgcaattt attgcgtggc cgtgaagcgt tgccggcgca gccggaaaac
     7141 ccgctgtcag cacgttcagg aagggggtga tgtacaaatg gcacagagga tgaaagtgac
     7201 gtatgagctg aaggaatgat gtaaaagtga tgtataagtg atgtaaaagt gatgtataag
     7261 tgatgtaaaa gtgatgtatg aactgaagga gcgatgtaat tctgtagtca ggaagaataa
     7321 cccgttcatg caggcatgaa cgatacagtg aggaacgctt tttttgtcac gatctcccct
     7381 taacctgcgt aaatcacccg ccatggttga ttatttttca acctcaaaat ggtcatacac
     7441 ctaacgcagc cgcgattttc atcgcaactg catgaattaa aagaaaaaaa aggaatattt
     7501 cgttttcacg agtaataccc gggtattaaa aatccactgt aattacatga aaataaagaa
     7561 ataaaaggta ataccggagt attaccacgt acgcgggcaa gcgaaaaagc tataacgctt
     7621 cccggccgac aggcgaccca aaatccacag gggcatgcag gttctcttca gtaacctgcg
     7681 ccattaaatc aaaaaggtca tattcgccgg gaagctgcgt atcagtcgaa tccagttgtt
     7741 catcatattc agatggcatg gggtgatatc cagtagctga gaggttgtta gcgggtttcg
     7801 gggcgcagcc ctgaaccagt catgtagcgc tagcggagtg tatactggct tactatgttg
     7861 ccacgattgc gaaaatcagt gaagtgcttc atgtggcagg agaaaaaagg cagcaccggc
     7921 gcgtcagcag aatatgcgat acaggagata ttccgcttcc tcgctcactg actcgctacg
     7981 ctcggtcgtt cggctgcggc gagcggtacc ggcttacgga cggggacgga aatttcctgg
     8041 aagataccag gaagacactt agcaaagaag agataaggcc acggcgaagc cgtttttcca
     8101 taggctccgc ccccctgaca agcatcgcaa aatctgacgc tcaaatcagt ggtggcgaaa
     8161 cccgacagga ctataaagat cccaggcgtt tccccctggt agctccctcg tgcgctctcc
     8221 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcggt ttctcattcc
     8281 acgcctgaca ctcagttccg tgaaggcagt tcgctccaag ctggactgta tgcacgaacc
     8341 ccccgttcag tccgaccgct gcgccttgtc cggtaactat caacttgagt ccaacccgga
     8401 a
//
