LOCUS       NZ_OP038291             8390 bp    DNA     circular CON 07-JAN-2024
DEFINITION  Shigella sonnei strain 202008564 plasmid p202008564-7, complete
            sequence.
ACCESSION   NZ_OP038291
VERSION     NZ_OP038291.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Recent and rapid emergence of extensively drug-resistant Shigella
            sonnei
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8390)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2022) Enteric Bacterial Pathogens Unit, Institut
            Pasteur, 28 rue du Dr Roux, Paris 75724, France
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Sequencing Technology             :: MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/07/2024 04:13:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 12
            CDSs (total)                      :: 12
            Genes (coding)                    :: 11
            CDSs (with protein)               :: 11
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP038291.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="202008564"
                     /isolation_source="human"
                     /db_xref="taxon:624"
                     /plasmid="p202008564-7"
     gene            join(8290..8390,1..49)
                     /locus_tag="OVO84_RS00005"
     CDS             join(8290..8390,1..49)
                     /locus_tag="OVO84_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            255..746
                     /locus_tag="OVO84_RS00010"
                     /old_locus_tag="p202008564-7__00001"
     CDS             255..746
                     /locus_tag="OVO84_RS00010"
                     /old_locus_tag="p202008564-7__00001"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_267191397.1"
                     /translation="MAGDLRRLRGDRDKKSVPHCIVHACMNGLFFLTTELHRSFSSYIT
                     YTSLLHHLYITFTSFLQLIRHFHPLCHLYITPFLNVLTAGFPAAPATLHGHAINCVPVH
                     RFRMGRTPETGLTPAEWCEFPRYALVRILMHAVKTEPARFWRVFGWFVACFTGFPSETP
                     "
                     /name="hypothetical protein"
     gene            complement(805..1245)
                     /locus_tag="OVO84_RS00015"
                     /old_locus_tag="p202008564-7__00002"
     CDS             complement(805..1245)
                     /locus_tag="OVO84_RS00015"
                     /old_locus_tag="p202008564-7__00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            1430..1852
                     /locus_tag="OVO84_RS00020"
                     /old_locus_tag="p202008564-7__00003"
     CDS             1430..1852
                     /locus_tag="OVO84_RS00020"
                     /old_locus_tag="p202008564-7__00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            1896..>2084
                     /locus_tag="OVO84_RS00025"
                     /old_locus_tag="p202008564-7__00004"
                     /pseudo=""
     CDS             1896..>2084
                     /locus_tag="OVO84_RS00025"
                     /old_locus_tag="p202008564-7__00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(2051..3250)
                     /gene="tet(A)"
                     /locus_tag="OVO84_RS00030"
                     /old_locus_tag="p202008564-7__00005"
     ARG             complement(2055..3250)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3356..4006
                     /gene="tetR(A)"
                     /locus_tag="OVO84_RS00035"
                     /old_locus_tag="p202008564-7__00006"
     CDS             3356..4006
                     /gene="tetR(A)"
                     /locus_tag="OVO84_RS00035"
                     /old_locus_tag="p202008564-7__00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            complement(4038..4280)
                     /locus_tag="OVO84_RS00040"
                     /old_locus_tag="p202008564-7__00007"
     CDS             complement(4038..4280)
                     /locus_tag="OVO84_RS00040"
                     /old_locus_tag="p202008564-7__00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(4586..5422)
                     /locus_tag="OVO84_RS00045"
                     /old_locus_tag="p202008564-7__00008"
     CDS             complement(4586..5422)
                     /locus_tag="OVO84_RS00045"
                     /old_locus_tag="p202008564-7__00008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(5422..6225)
                     /gene="aph(3'')-Ib"
                     /locus_tag="OVO84_RS00050"
                     /old_locus_tag="p202008564-7__00009"
     ARG             complement(5426..6225)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            complement(6286..7101)
                     /gene="sul2"
                     /locus_tag="OVO84_RS00055"
                     /old_locus_tag="p202008564-7__00010"
     ARG             complement(6290..7101)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(7561..7719)
                     /locus_tag="OVO84_RS00060"
     CDS             complement(7561..7719)
                     /locus_tag="OVO84_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     ARG             complement(4590..5422)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          8082..8255
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(7553..8158)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gcatgcccct gtggattttg ggtcgcctgt cggccgggaa gcgttatagc tttttcgctt
       61 gcccgcgtac gtggtaatac tccggtatta ccttttattt ctttattttc atgtaattac
      121 agtggatttt taatacccgg gtattactcg tgaaaacgaa atattccttt tttttctttt
      181 aattcatgca gttgcgatga aaatcgcggc tgcgttaggt gtatgaccat tttgaggttg
      241 aaaaataatc aaccatggcg ggtgatttac gcaggttaag gggagatcgt gacaaaaaaa
      301 gcgttcctca ctgtatcgtt catgcctgca tgaacgggtt attcttcctg actacagaat
      361 tacatcgctc cttcagttca tacatcactt atacatcact tttacatcac ttatacatca
      421 cttttacatc attccttcag ctcatacgtc actttcatcc tctgtgccat ttgtacatca
      481 cccccttcct gaacgtgctg acagcgggtt ttccggctgc gccggcaacg cttcacggcc
      541 acgcaataaa ttgcgtcccc gttcaccgtt ttcggatggg aagaacgccg gaaacaggac
      601 ttaccccggc tgaatggtgt gaatttccgc gctatgcact tgtgcgcata ctcatgcatg
      661 ccgtaaaaac agagcctgcg cgtttctggc gggttttcgg gtggtttgtt gcctgtttta
      721 ccggtttccc gtcagaaacg ccctgagggc ctctcaggcg gtgctgataa cagatgttat
      781 ggtaaatcag agtcacctgc actgttactc tgaaccggag cgaaacagcc gcgaccagaa
      841 tccccgcttt ttttcctgtg ccagcgcgtc agccagacgg ctaatttcgg cctgtagttg
      901 ttccctctcc tcatttcgca ggcggttttc tgcttcccgc gccatcttgt cttcaagcat
      961 tagggtaaca gtctgctgca gagcgtccag tttctgctga atgacgtcta gctgagcatt
     1021 ttccgcctgg ctaacgacag atttggagtg tccctccttg tgcctttttg gtgtctcaga
     1081 agtgcgcagc tcgccatagg cccgaatcag ttcactggtc tcgaactccc ggcgatcatt
     1141 ttttcctgtg cgatagctga caaggccttt agccatgtca cgatataatt gcgagcgcga
     1201 ctttcctgtt aatttcagtg tctcttgaac tgtgtgccat gccataaaag tgacagtgtc
     1261 tcctgtgtgt accgtattgt aacagcagaa catattactg ttttaacaca aaaggtctga
     1321 ggctgcccgt gaggcgtgaa aaatacacaa aaaacctgtt gacggtgtgg ttttgacggg
     1381 cgtagtattt cgaatactgt atataaacac attgttataa atacagtttt tgctgttttt
     1441 aaaagtcaaa gaggataatt ttatgagtac agagttaaat agcggtactg tcggtggttt
     1501 tggtcacatg ggaccaaatg acccctacaa tgatggtcat agcggctctg gaagttcggg
     1561 aggtagtttt aacggtggtg gtaatggtgg ccatgataat agtagtggtt catcttatac
     1621 tgcatccttt ggtctcatca ctggccgaca cccgataact gtcccaggta cgaagaatat
     1681 taattctgtc gatctttctg ctgtcattaa taacattata catcatgaag gtgcacgcaa
     1741 taacatgtac ctagatactg cgaacaatgt aagagtggga gtgacgggca ctggctggca
     1801 atgtctagca acggcaggca tttcggctga gggtaaaaga actttccgct aagcgataga
     1861 ctgtatgtaa acacagtatt gcaaggacgc ggaacatgcc tcatgtggcg gccaggacgg
     1921 ccagccggga tcgggatact ggtcgttacc agagccaccg acccgagcaa acccttctct
     1981 atcagatcgt tgacgagtat tacccggcat tcgctgcgct tatggcagag cagggaaagg
     2041 aattgccggg ctatgtgcaa cgggaatttg aagaatttct ccaaagcccg cgacgcagcg
     2101 ccggcaggca gagcaagtag agggcagcgc ctgcaatcca tgcccacccg ttccacgttg
     2161 ttatagaagc cgcatagatc gccgtgaaga ggaggggtcc gacgatcgag gtcaggctgg
     2221 tgagcgccgc cagtgagcct tgcagctgcc cctgacgttc ctcatccacc tgcctggaca
     2281 acattgcttg cagcgccggc attccgatgc cacccgaagc aagcaggacc atgatcggga
     2341 acgccatcca tccccgtgtc gcgaaggcaa gcaggatgta gcctgtgccg tcggcaatca
     2401 ttccgagcat gagtgcccgc ctttcgccga gccgggcggc tacagggccg gtgatcattg
     2461 cctgggcgag tgaatgcaga atgccaaatg cggcaagcga aatgccgatc gtggtcgcgt
     2521 cccagtgaaa gcgatcctcg ccgaaaatga cccaaagcgc ggccggcacc tgtccgacaa
     2581 gttgcatgat gaagaagacc gccatcaggg cggcgacgac ggtcatgccc cgggcccacc
     2641 ggaacgaagc gagcgggttg agagcctccc ggcgtaacgg ccggcgttcg cctttgtgcg
     2701 actccggcaa aaggaaacag cccgtcagga aattgaggcc gttcaaggct gccgcggcga
     2761 agaacggagc gtggggggag aaaccgccca tcagcccacc gagcacaggt cccgcgacca
     2821 tcccgaaccc gaaacaggcg ctcatgaagc cgaagtgccg cgcgcgctca tcgccatcag
     2881 tgatatcggc aatataagcg ccggctaccg ccccagtcgc cccggtgatg ccggccacga
     2941 tccgcccgat atagagaacc caaaggaaag gcgccgtcgc catgatggcg tagtcgacag
     3001 cagcgccggc cagcgagacg agcaagaccg gccgccgccc gaaacgatcc gacagcgcgc
     3061 ccagcacagg tgcgcaggca aattgcatca acgcatacag cgccagcaga atgccatagt
     3121 gggcggtgac gtcgttcgag tgaaccagat cgcgcaggag gcccggcagc accggcataa
     3181 tcaggccgat gccgacagcg tcgagcgcga cagtgctcag aattacgatc aggggtctgt
     3241 tgggtttcac gtctggcctc cggaccagcc tccgctggtc cgattgaacg cgcggattct
     3301 ttatcactga taagttggtg gacatattat gtttatcagt gataaagtgt caagcatgac
     3361 aaagttgcag ccgaatacag tgatccgtgc cgccctggac ctgttgaacg aggtcggcgt
     3421 agacggtctg acgacacgca aactggcgga acggttgggg gttcagcagc cggcgcttta
     3481 ctggcacttc aggaacaagc gggcgctgct cgacgcactg gccgaagcca tgctggcgga
     3541 gaatcatacg cattcggtgc cgagagccga cgacgactgg cgctcatttc tgatcgggaa
     3601 tgcccgcagc ttcaggcagg cgctgctcgc ctaccgcgat ggcgcgcgca tccatgccgg
     3661 cacgcgaccg ggcgcaccgc agatggaaac ggccgacgcg cagcttcgct tcctctgcga
     3721 ggcgggtttt tcggccgggg acgccgtcaa tgcgctgatg acaatcagct acttcactgt
     3781 tggggccgtg cttgaggagc aggccggcga cagcgatgcc ggcgagcgcg gcggcaccgt
     3841 tgaacaggct ccgctctcgc cgctgttgcg ggccgcgata gacgccttcg acgaagccgg
     3901 tccggacgca gcgttcgagc agggactcgc ggtgattgtc gatggattgg cgaaaaggag
     3961 gctcgttgtc aggaacgttg aaggaccgag aaagggtgac gattgatcag gaccgctgcc
     4021 ggagcgcaac ccactcacta cagcagagcc atgtagacaa catcccctcc ccctttccac
     4081 cgcgtcagac gcccgtagca gcccgctacg ggctttttca tgccctgccc tagcgtccaa
     4141 gcctcacggc cgcgctcggc ctctctggcg gccttctggc gctcctgctg cggcgtccgc
     4201 tcgtgggccg tggcgcgggt ccgcgcgccg gcctcgtgcg cctggcgctc gcgggcgagg
     4261 tccagggcgg ccgtcttcac gttctgcctt gcgcagatga gatagatcta gcgtggactc
     4321 aaggctctcg cgaatggctc gcgttggaaa ctttcattga cacttgaggg gcaccgcagg
     4381 gaaattctcg tccttgcgag aaccggctat gtcgtgctgc gcatcgagcc tgcgcccttg
     4441 gcttgtctcg cccctctccg cgtcgctacg gggcttccag gggtcgtttg cgggaagggg
     4501 cggaatccta cgctaaggct ttggccagcg atattctccg gtgagattga tgtgttccca
     4561 ggggatagga gaagtcgctt gatatctagt atgacgtctg tcgcacctgc ttgatcgcgg
     4621 ccgcgatagc tagatcgcgt tgctcctctt ctccatccgc gttccaagct gcggaaaggc
     4681 acccataagc gtacgcctgg tcgagcaggc gacgcggatc gacgtccagc gcacgagaga
     4741 atgcgtccgc catctgtgca atgcgtctag gatcgagaca aaggtcgtct ctgtcagccg
     4801 gatcgtagaa catattggcg gcgccaaagc ccacttcacc gaccagaccg acgggatcta
     4861 tcaccagcca gccgcgactg gagaacatga tgttttcatg atgcagatcg ccatgtagcc
     4921 cacgcagttc cgaggcattg ctcatcattt gatcggctat aatcgccgcg tggacgtagt
     4981 cagtttgaca acctgcgttt tgatcatcgc gcgcccgctg aaacaaagct gcaaagcgat
     5041 cccggatcgg gagaagggca gaaggcaggg gttcctcaga tgcggcatac agcttcgcca
     5101 ttagttccgc tgcaatttcg gtcgcctggt agtcgccgtg ctcggcaacg atgtgagaga
     5161 gcattcgctc cccggcatat tcgagcaaca tcagattgtt ctcacgaccg agcaaccgga
     5221 ctgctcccct cccattgcgc cataccagat agtcggcccc gcgcagttca tcagcaatgt
     5281 cttctatagg tttcaatccc ttgacgattg caggagtccc gtctggcaat gaaactttcc
     5341 aaacgaggct ggaaaaggtg tccgcaatga gaacaggttg cgaaacgtgc caatgagcag
     5401 gaaaaacagg cggcatgaac atcaacccca agtcagaggg tccaatcgca gatagaaggc
     5461 aaggcgttcg cggtcggggg cttcgatccc caatacattg aataggacag cgaaggcgcg
     5521 ctctgcttca tctggcgctg cccagttctc ttcggcgtta gcaatcatga gtgccaaatc
     5581 ggcatagcga tctgctgttc cgagccgccc aaggtcgatc agacccgtgc attgaagagt
     5641 tttagggtcc accatgaagt tcggcatgca gggatcacca tggcaaacaa ccatatcggt
     5701 gcgctcttgg tcgagccgca ccggtagctc tcgttcgaca cgagccaaaa gatcgagctg
     5761 cggcgtactc ttgtcctcgt ccggtaagaa gtcgggattg acggcattgc gggacaccac
     5821 atcaacggcg cgtccgaaca ttcgcgacag cctgcgctca aactgacatt gatcaaccga
     5881 taggctgtga acagcgccaa gttgctgccc cattgacggc cacgctttga gcaaatccgc
     5941 tccagacaga tcagccgccg gtactcccgg aattgccgtt atcaccaagc atgcaccctc
     6001 ctgttcctcc tgccagttga tgacctcggg gcaagccaca cctcgacctt tgagccaaat
     6061 gaggcggtca cgctctccag cgagctcacc gcggcgggaa gcaggtgcga ttttcgcgaa
     6121 ggcatgcccg tcaccacgtc gaaaaacaaa atcaccagat tctccgcctc tgacaggcaa
     6181 ccagtcagaa tgcgattcac caaaaaaaat attagttcga ttcaatggag gttccttcag
     6241 ttttctgatg aagcgcgaat atagagaaat atcccgaatg tgcagttaac gaattcttgc
     6301 ggtttctttc agcgccgcca ataccgccag cccgtcgcgc aaggggcgcg gctcgtgtgt
     6361 gcggatgaag tcagctccac ctgcggcggc ggcaagctct gcagcgagtg tcgcggcccc
     6421 gacatccccc ggaccacggc ctgtgagcgc gcgcagaaag gatttgcgcg aaacagacag
     6481 aagcaccggc aaatcgaagc gcagccgcaa ttcatcgaac cgcgccagca ccgagagcga
     6541 ggtttcggga gcagccccca gaaaaaaccc catgccggga tcaaggacaa ggcggttgcg
     6601 tttgataccg gcacccgtca gcgccgcgat gcgcgcgtca aagaacgccg caatgtgatc
     6661 catgatgtcg ccagcgggtg cctcgcgccg atctgcctgc ccgtcttgca ccgaatgcat
     6721 aacgacgagt ttggcagatg atttcgccaa ttgcggatag aacgcagcgt ctggaaaacc
     6781 gcgaatatca ttgagatagg ccacaccacg cgacaaggca taggcttgcg tcgcgggttg
     6841 ataactgtcg agcgagacgg gaatgccatc tgccttgagc gcgtccagca ccggcgcgat
     6901 acgcgcgatt tctgtgtcgg acgaaacagg cgcggcgtcg ggattgctgg atgccggacc
     6961 gaggtcgatc acatctgccc cctcggccat cagcttacgc gcctgcgcaa tggctgcgtc
     7021 tggcgccaga taccggcctc catcggagaa actgtccgag gttatgttga cgatgccgaa
     7081 aatgatgagc gatttattca tgggggcttc tataataata ataatcgagc atgagtctca
     7141 tacggatgct cgggtcgaaa gggaatcccc aggcgagtaa cctgtttgcg gtgatccgtt
     7201 tcatgatgat gtatctgtag cattttggaa ataaatatat catctctcaa taagtagata
     7261 tatttatctt ttttgcttct ttggtgctgc aagtcaccaa tacgagggaa acctgttacg
     7321 gagcggttta accgtaatca acagtgactg gtgtcatgtt cggaagatta attgatagta
     7381 atctacaaag gatcttcttg agatcctttt caatgaatcg taatctcttg ctctgtaaac
     7441 gaaaaaaccg cctggggagg cggtttgttc gaaggttaag tcagttgggg aactgcttaa
     7501 cccggtaaca gggttcacga gaccgccatt accaaaactg ttctttcagt gtagccgcag
     7561 tcaggcctct acttcaagac ctcttaatct atcttctcgt gcactcctgt tactaatggc
     7621 tgctgccagt ggcgttttgt cgtgtctttc cgggttggac tcaagttgat agttaccgga
     7681 caaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
     7741 tgccttcacg gaactgagtg tcaggcgtgg aatgagaaac cgcggccata acagcggaat
     7801 gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagctac cagggggaaa
     7861 cgcctgggat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttt
     7921 gcgatgcttg tcaggggggc ggagcctatg gaaaaacggc ttcgccgtgg ccttatctct
     7981 tctttgctaa gtgtcttcct ggtatcttcc aggaaatttc cgtccccgtc cgtaagccgg
     8041 taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaata
     8101 tctcctgtat cgcatattct gctgacgcgc cggtgctgcc ttttttctcc tgccacatga
     8161 agcacttcac tgattttcgc aatcgtggca acatagtaag ccagtataca ctccgctagc
     8221 gctacatgac tggttcaggg ctgcgccccg aaacccgcta acaacctctc agctactgga
     8281 tatcacccca tgccatctga atatgatgaa caactggatt cgactgatac gcagcttccc
     8341 ggcgaatatg acctttttga tttaatggcg caggttactg aagagaacct
//
