LOCUS       NZ_OP038288             8390 bp    DNA     circular CON 07-JAN-2024
DEFINITION  Shigella sonnei strain 202008118 plasmid p202008118-7, complete
            sequence.
ACCESSION   NZ_OP038288
VERSION     NZ_OP038288.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Recent and rapid emergence of extensively drug-resistant Shigella
            sonnei
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8390)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2022) Enteric Bacterial Pathogens Unit, Institut
            Pasteur, 28 rue du Dr Roux, Paris 75724, France
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Sequencing Technology             :: MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/07/2024 04:05:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 12
            CDSs (total)                      :: 12
            Genes (coding)                    :: 11
            CDSs (with protein)               :: 11
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP038288.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="202008118"
                     /isolation_source="human"
                     /db_xref="taxon:624"
                     /plasmid="p202008118-7"
     gene            16..258
                     /locus_tag="OVO68_RS00005"
                     /old_locus_tag="p202008118-7__00001"
     CDS             16..258
                     /locus_tag="OVO68_RS00005"
                     /old_locus_tag="p202008118-7__00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(290..940)
                     /gene="tetR(A)"
                     /locus_tag="OVO68_RS00010"
                     /old_locus_tag="p202008118-7__00002"
     CDS             complement(290..940)
                     /gene="tetR(A)"
                     /locus_tag="OVO68_RS00010"
                     /old_locus_tag="p202008118-7__00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            1046..2245
                     /gene="tet(A)"
                     /locus_tag="OVO68_RS00015"
                     /old_locus_tag="p202008118-7__00003"
     ARG             1047..2242
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<2212..2400)
                     /locus_tag="OVO68_RS00020"
                     /old_locus_tag="p202008118-7__00004"
                     /pseudo=""
     CDS             complement(<2212..2400)
                     /locus_tag="OVO68_RS00020"
                     /old_locus_tag="p202008118-7__00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(2444..2866)
                     /locus_tag="OVO68_RS00025"
                     /old_locus_tag="p202008118-7__00005"
     CDS             complement(2444..2866)
                     /locus_tag="OVO68_RS00025"
                     /old_locus_tag="p202008118-7__00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            3051..3491
                     /locus_tag="OVO68_RS00030"
                     /old_locus_tag="p202008118-7__00006"
     CDS             3051..3491
                     /locus_tag="OVO68_RS00030"
                     /old_locus_tag="p202008118-7__00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(3550..4041)
                     /locus_tag="OVO68_RS00035"
                     /old_locus_tag="p202008118-7__00007"
     CDS             complement(3550..4041)
                     /locus_tag="OVO68_RS00035"
                     /old_locus_tag="p202008118-7__00007"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_267191397.1"
                     /translation="MAGDLRRLRGDRDKKSVPHCIVHACMNGLFFLTTELHRSFSSYIT
                     YTSLLHHLYITFTSFLQLIRHFHPLCHLYITPFLNVLTAGFPAAPATLHGHAINCVPVH
                     RFRMGRTPETGLTPAEWCEFPRYALVRILMHAVKTEPARFWRVFGWFVACFTGFPSETP
                     "
                     /name="hypothetical protein"
     gene            complement(4247..4396)
                     /locus_tag="OVO68_RS00040"
                     /old_locus_tag="p202008118-7__00008"
     CDS             complement(4247..4396)
                     /locus_tag="OVO68_RS00040"
                     /old_locus_tag="p202008118-7__00008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            4967..5125
                     /locus_tag="OVO68_RS00060"
     CDS             4967..5125
                     /locus_tag="OVO68_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            5585..6400
                     /gene="sul2"
                     /locus_tag="OVO68_RS00045"
                     /old_locus_tag="p202008118-7__00009"
     ARG             5586..6397
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6461..7264
                     /gene="aph(3'')-Ib"
                     /locus_tag="OVO68_RS00050"
                     /old_locus_tag="p202008118-7__00010"
     ARG             6462..7261
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            7264..8100
                     /locus_tag="OVO68_RS00055"
                     /old_locus_tag="p202008118-7__00011"
     CDS             7264..8100
                     /locus_tag="OVO68_RS00055"
                     /old_locus_tag="p202008118-7__00011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     ARG             7265..8097
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(4432..4605)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          4529..5134
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tgcgcaaggc agaacgtgaa gacggccgcc ctggacctcg cccgcgagcg ccaggcgcac
       61 gaggccggcg cgcggacccg cgccacggcc cacgagcgga cgccgcagca ggagcgccag
      121 aaggccgcca gagaggccga gcgcggccgt gaggcttgga cgctagggca gggcatgaaa
      181 aagcccgtag cgggctgcta cgggcgtctg acgcggtgga aagggggagg ggatgttgtc
      241 tacatggctc tgctgtagtg agtgggttgc gctccggcag cggtcctgat caatcgtcac
      301 cctttctcgg tccttcaacg ttcctgacaa cgagcctcct tttcgccaat ccatcgacaa
      361 tcaccgcgag tccctgctcg aacgctgcgt ccggaccggc ttcgtcgaag gcgtctatcg
      421 cggcccgcaa cagcggcgag agcggagcct gttcaacggt gccgccgcgc tcgccggcat
      481 cgctgtcgcc ggcctgctcc tcaagcacgg ccccaacagt gaagtagctg attgtcatca
      541 gcgcattgac ggcgtccccg gccgaaaaac ccgcctcgca gaggaagcga agctgcgcgt
      601 cggccgtttc catctgcggt gcgcccggtc gcgtgccggc atggatgcgc gcgccatcgc
      661 ggtaggcgag cagcgcctgc ctgaagctgc gggcattccc gatcagaaat gagcgccagt
      721 cgtcgtcggc tctcggcacc gaatgcgtat gattctccgc cagcatggct tcggccagtg
      781 cgtcgagcag cgcccgcttg ttcctgaagt gccagtaaag cgccggctgc tgaaccccca
      841 accgttccgc cagtttgcgt gtcgtcagac cgtctacgcc gacctcgttc aacaggtcca
      901 gggcggcacg gatcactgta ttcggctgca actttgtcat gcttgacact ttatcactga
      961 taaacataat atgtccacca acttatcagt gataaagaat ccgcgcgttc aatcggacca
     1021 gcggaggctg gtccggaggc cagacgtgaa acccaacaga cccctgatcg taattctgag
     1081 cactgtcgcg ctcgacgctg tcggcatcgg cctgattatg ccggtgctgc cgggcctcct
     1141 gcgcgatctg gttcactcga acgacgtcac cgcccactat ggcattctgc tggcgctgta
     1201 tgcgttgatg caatttgcct gcgcacctgt gctgggcgcg ctgtcggatc gtttcgggcg
     1261 gcggccggtc ttgctcgtct cgctggccgg cgctgctgtc gactacgcca tcatggcgac
     1321 ggcgcctttc ctttgggttc tctatatcgg gcggatcgtg gccggcatca ccggggcgac
     1381 tggggcggta gccggcgctt atattgccga tatcactgat ggcgatgagc gcgcgcggca
     1441 cttcggcttc atgagcgcct gtttcgggtt cgggatggtc gcgggacctg tgctcggtgg
     1501 gctgatgggc ggtttctccc cccacgctcc gttcttcgcc gcggcagcct tgaacggcct
     1561 caatttcctg acgggctgtt tccttttgcc ggagtcgcac aaaggcgaac gccggccgtt
     1621 acgccgggag gctctcaacc cgctcgcttc gttccggtgg gcccggggca tgaccgtcgt
     1681 cgccgccctg atggcggtct tcttcatcat gcaacttgtc ggacaggtgc cggccgcgct
     1741 ttgggtcatt ttcggcgagg atcgctttca ctgggacgcg accacgatcg gcatttcgct
     1801 tgccgcattt ggcattctgc attcactcgc ccaggcaatg atcaccggcc ctgtagccgc
     1861 ccggctcggc gaaaggcggg cactcatgct cggaatgatt gccgacggca caggctacat
     1921 cctgcttgcc ttcgcgacac ggggatggat ggcgttcccg atcatggtcc tgcttgcttc
     1981 gggtggcatc ggaatgccgg cgctgcaagc aatgttgtcc aggcaggtgg atgaggaacg
     2041 tcaggggcag ctgcaaggct cactggcggc gctcaccagc ctgacctcga tcgtcggacc
     2101 cctcctcttc acggcgatct atgcggcttc tataacaacg tggaacgggt gggcatggat
     2161 tgcaggcgct gccctctact tgctctgcct gccggcgctg cgtcgcgggc tttggagaaa
     2221 ttcttcaaat tcccgttgca catagcccgg caattccttt ccctgctctg ccataagcgc
     2281 agcgaatgcc gggtaatact cgtcaacgat ctgatagaga agggtttgct cgggtcggtg
     2341 gctctggtaa cgaccagtat cccgatcccg gctggccgtc ctggccgcca catgaggcat
     2401 gttccgcgtc cttgcaatac tgtgtttaca tacagtctat cgcttagcgg aaagttcttt
     2461 taccctcagc cgaaatgcct gccgttgcta gacattgcca gccagtgccc gtcactccca
     2521 ctcttacatt gttcgcagta tctaggtaca tgttattgcg tgcaccttca tgatgtataa
     2581 tgttattaat gacagcagaa agatcgacag aattaatatt cttcgtacct gggacagtta
     2641 tcgggtgtcg gccagtgatg agaccaaagg atgcagtata agatgaacca ctactattat
     2701 catggccacc attaccacca ccgttaaaac tacctcccga acttccagag ccgctatgac
     2761 catcattgta ggggtcattt ggtcccatgt gaccaaaacc accgacagta ccgctattta
     2821 actctgtact cataaaatta tcctctttga cttttaaaaa cagcaaaaac tgtatttata
     2881 acaatgtgtt tatatacagt attcgaaata ctacgcccgt caaaaccaca ccgtcaacag
     2941 gttttttgtg tatttttcac gcctcacggg cagcctcaga ccttttgtgt taaaacagta
     3001 atatgttctg ctgttacaat acggtacaca caggagacac tgtcactttt atggcatggc
     3061 acacagttca agagacactg aaattaacag gaaagtcgcg ctcgcaatta tatcgtgaca
     3121 tggctaaagg ccttgtcagc tatcgcacag gaaaaaatga tcgccgggag ttcgagacca
     3181 gtgaactgat tcgggcctat ggcgagctgc gcacttctga gacaccaaaa aggcacaagg
     3241 agggacactc caaatctgtc gttagccagg cggaaaatgc tcagctagac gtcattcagc
     3301 agaaactgga cgctctgcag cagactgtta ccctaatgct tgaagacaag atggcgcggg
     3361 aagcagaaaa ccgcctgcga aatgaggaga gggaacaact acaggccgaa attagccgtc
     3421 tggctgacgc gctggcacag gaaaaaaagc ggggattctg gtcgcggctg tttcgctccg
     3481 gttcagagta acagtgcagg tgactctgat ttaccataac atctgttatc agcaccgcct
     3541 gagaggccct cagggcgttt ctgacgggaa accggtaaaa caggcaacaa accacccgaa
     3601 aacccgccag aaacgcgcag gctctgtttt tacggcatgc atgagtatgc gcacaagtgc
     3661 atagcgcgga aattcacacc attcagccgg ggtaagtcct gtttccggcg ttcttcccat
     3721 ccgaaaacgg tgaacgggga cgcaatttat tgcgtggccg tgaagcgttg ccggcgcagc
     3781 cggaaaaccc gctgtcagca cgttcaggaa gggggtgatg tacaaatggc acagaggatg
     3841 aaagtgacgt atgagctgaa ggaatgatgt aaaagtgatg tataagtgat gtaaaagtga
     3901 tgtataagtg atgtatgaac tgaaggagcg atgtaattct gtagtcagga agaataaccc
     3961 gttcatgcag gcatgaacga tacagtgagg aacgcttttt ttgtcacgat ctccccttaa
     4021 cctgcgtaaa tcacccgcca tggttgatta tttttcaacc tcaaaatggt catacaccta
     4081 acgcagccgc gattttcatc gcaactgcat gaattaaaag aaaaaaaagg aatatttcgt
     4141 tttcacgagt aatacccggg tattaaaaat ccactgtaat tacatgaaaa taaagaaata
     4201 aaaggtaata ccggagtatt accacgtacg cgggcaagcg aaaaagctat aacgcttccc
     4261 ggccgacagg cgacccaaaa tccacagggg catgcaggtt ctcttcagta acctgcgcca
     4321 ttaaatcaaa aaggtcatat tcgccgggaa gctgcgtatc agtcgaatcc agttgttcat
     4381 catattcaga tggcatgggg tgatatccag tagctgagag gttgttagcg ggtttcgggg
     4441 cgcagccctg aaccagtcat gtagcgctag cggagtgtat actggcttac tatgttgcca
     4501 cgattgcgaa aatcagtgaa gtgcttcatg tggcaggaga aaaaaggcag caccggcgcg
     4561 tcagcagaat atgcgataca ggagatattc cgcttcctcg ctcactgact cgctacgctc
     4621 ggtcgttcgg ctgcggcgag cggtaccggc ttacggacgg ggacggaaat ttcctggaag
     4681 ataccaggaa gacacttagc aaagaagaga taaggccacg gcgaagccgt ttttccatag
     4741 gctccgcccc cctgacaagc atcgcaaaat ctgacgctca aatcagtggt ggcgaaaccc
     4801 gacaggacta taaagatccc aggcgtttcc ccctggtagc tccctcgtgc gctctcctgt
     4861 tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcggtttc tcattccacg
     4921 cctgacactc agttccgtga aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     4981 cgttcagtcc gaccgctgcg ccttgtccgg taactatcaa cttgagtcca acccggaaag
     5041 acacgacaaa acgccactgg cagcagccat tagtaacagg agtgcacgag aagatagatt
     5101 aagaggtctt gaagtagagg cctgactgcg gctacactga aagaacagtt ttggtaatgg
     5161 cggtctcgtg aaccctgtta ccgggttaag cagttcccca actgacttaa ccttcgaaca
     5221 aaccgcctcc ccaggcggtt ttttcgttta cagagcaaga gattacgatt cattgaaaag
     5281 gatctcaaga agatcctttg tagattacta tcaattaatc ttccgaacat gacaccagtc
     5341 actgttgatt acggttaaac cgctccgtaa caggtttccc tcgtattggt gacttgcagc
     5401 accaaagaag caaaaaagat aaatatatct acttattgag agatgatata tttatttcca
     5461 aaatgctaca gatacatcat catgaaacgg atcaccgcaa acaggttact cgcctgggga
     5521 ttccctttcg acccgagcat ccgtatgaga ctcatgctcg attattatta ttatagaagc
     5581 ccccatgaat aaatcgctca tcattttcgg catcgtcaac ataacctcgg acagtttctc
     5641 cgatggaggc cggtatctgg cgccagacgc agccattgcg caggcgcgta agctgatggc
     5701 cgagggggca gatgtgatcg acctcggtcc ggcatccagc aatcccgacg ccgcgcctgt
     5761 ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg gacgcgctca aggcagatgg
     5821 cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa gcctatgcct tgtcgcgtgg
     5881 tgtggcctat ctcaatgata ttcgcggttt tccagacgct gcgttctatc cgcaattggc
     5941 gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa gacgggcagg cagatcggcg
     6001 cgaggcaccc gctggcgaca tcatggatca cattgcggcg ttctttgacg cgcgcatcgc
     6061 ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc cttgatcccg gcatggggtt
     6121 ttttctgggg gctgctcccg aaacctcgct ctcggtgctg gcgcggttcg atgaattgcg
     6181 gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc aaatcctttc tgcgcgcgct
     6241 cacaggccgt ggtccggggg atgtcggggc cgcgacactc gctgcagagc ttgccgccgc
     6301 cgcaggtgga gctgacttca tccgcacaca cgagccgcgc cccttgcgcg acgggctggc
     6361 ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa ctgcacattc gggatatttc
     6421 tctatattcg cgcttcatca gaaaactgaa ggaacctcca ttgaatcgaa ctaatatttt
     6481 ttttggtgaa tcgcattctg actggttgcc tgtcagaggc ggagaatctg gtgattttgt
     6541 ttttcgacgt ggtgacgggc atgccttcgc gaaaatcgca cctgcttccc gccgcggtga
     6601 gctcgctgga gagcgtgacc gcctcatttg gctcaaaggt cgaggtgtgg cttgccccga
     6661 ggtcatcaac tggcaggagg aacaggaggg tgcatgcttg gtgataacgg caattccggg
     6721 agtaccggcg gctgatctgt ctggagcgga tttgctcaaa gcgtggccgt caatggggca
     6781 gcaacttggc gctgttcaca gcctatcggt tgatcaatgt cagtttgagc gcaggctgtc
     6841 gcgaatgttc ggacgcgccg ttgatgtggt gtcccgcaat gccgtcaatc ccgacttctt
     6901 accggacgag gacaagagta cgccgcagct cgatcttttg gctcgtgtcg aacgagagct
     6961 accggtgcgg ctcgaccaag agcgcaccga tatggttgtt tgccatggtg atccctgcat
     7021 gccgaacttc atggtggacc ctaaaactct tcaatgcacg ggtctgatcg accttgggcg
     7081 gctcggaaca gcagatcgct atgccgattt ggcactcatg attgctaacg ccgaagagaa
     7141 ctgggcagcg ccagatgaag cagagcgcgc cttcgctgtc ctattcaatg tattggggat
     7201 cgaagccccc gaccgcgaac gccttgcctt ctatctgcga ttggaccctc tgacttgggg
     7261 ttgatgttca tgccgcctgt ttttcctgct cattggcacg tttcgcaacc tgttctcatt
     7321 gcggacacct tttccagcct cgtttggaaa gtttcattgc cagacgggac tcctgcaatc
     7381 gtcaagggat tgaaacctat agaagacatt gctgatgaac tgcgcggggc cgactatctg
     7441 gtatggcgca atgggagggg agcagtccgg ttgctcggtc gtgagaacaa tctgatgttg
     7501 ctcgaatatg ccggggagcg aatgctctct cacatcgttg ccgagcacgg cgactaccag
     7561 gcgaccgaaa ttgcagcgga actaatggcg aagctgtatg ccgcatctga ggaacccctg
     7621 ccttctgccc ttctcccgat ccgggatcgc tttgcagctt tgtttcagcg ggcgcgcgat
     7681 gatcaaaacg caggttgtca aactgactac gtccacgcgg cgattatagc cgatcaaatg
     7741 atgagcaatg cctcggaact gcgtgggcta catggcgatc tgcatcatga aaacatcatg
     7801 ttctccagtc gcggctggct ggtgatagat cccgtcggtc tggtcggtga agtgggcttt
     7861 ggcgccgcca atatgttcta cgatccggct gacagagacg acctttgtct cgatcctaga
     7921 cgcattgcac agatggcgga cgcattctct cgtgcgctgg acgtcgatcc gcgtcgcctg
     7981 ctcgaccagg cgtacgctta tgggtgcctt tccgcagctt ggaacgcgga tggagaagag
     8041 gagcaacgcg atctagctat cgcggccgcg atcaagcagg tgcgacagac gtcatactag
     8101 atatcaagcg acttctccta tcccctggga acacatcaat ctcaccggag aatatcgctg
     8161 gccaaagcct tagcgtagga ttccgcccct tcccgcaaac gacccctgga agccccgtag
     8221 cgacgcggag aggggcgaga caagccaagg gcgcaggctc gatgcgcagc acgacatagc
     8281 cggttctcgc aaggacgaga atttccctgc ggtgcccctc aagtgtcaat gaaagtttcc
     8341 aacgcgagcc attcgcgaga gccttgagtc cacgctagat ctatctcatc
//
