LOCUS       NZ_OP038285             8379 bp    DNA     circular CON 07-JAN-2024
DEFINITION  Shigella sonnei strain 202000562 plasmid p202000562-5, complete
            sequence.
ACCESSION   NZ_OP038285
VERSION     NZ_OP038285.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Recent and rapid emergence of extensively drug-resistant Shigella
            sonnei
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2022) Enteric Bacterial Pathogens Unit, Institut
            Pasteur, 28 rue du Dr Roux, Paris 75724, France
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Sequencing Technology             :: MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/07/2024 04:08:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP038285.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="202000562"
                     /isolation_source="human"
                     /db_xref="taxon:624"
                     /plasmid="p202000562-5"
     gene            complement(join(<8336..8379,1..145))
                     /locus_tag="OVO69_RS00005"
                     /pseudo=""
     CDS             complement(join(<8336..8379,1..145))
                     /locus_tag="OVO69_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(189..611)
                     /locus_tag="OVO69_RS00010"
                     /old_locus_tag="p202000562-5__00001"
     CDS             complement(189..611)
                     /locus_tag="OVO69_RS00010"
                     /old_locus_tag="p202000562-5__00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            796..1236
                     /locus_tag="OVO69_RS00015"
                     /old_locus_tag="p202000562-5__00002"
     CDS             796..1236
                     /locus_tag="OVO69_RS00015"
                     /old_locus_tag="p202000562-5__00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(1981..2130)
                     /locus_tag="OVO69_RS00020"
                     /old_locus_tag="p202000562-5__00003"
     CDS             complement(1981..2130)
                     /locus_tag="OVO69_RS00020"
                     /old_locus_tag="p202000562-5__00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            2701..2859
                     /locus_tag="OVO69_RS00055"
     CDS             2701..2859
                     /locus_tag="OVO69_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            3319..4134
                     /gene="sul2"
                     /locus_tag="OVO69_RS00025"
                     /old_locus_tag="p202000562-5__00004"
     ARG             3320..4131
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4195..4998
                     /gene="aph(3'')-Ib"
                     /locus_tag="OVO69_RS00030"
                     /old_locus_tag="p202000562-5__00005"
     ARG             4196..4995
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4998..5834
                     /locus_tag="OVO69_RS00035"
                     /old_locus_tag="p202000562-5__00006"
     CDS             4998..5834
                     /locus_tag="OVO69_RS00035"
                     /old_locus_tag="p202000562-5__00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480972.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            6140..6382
                     /locus_tag="OVO69_RS00040"
                     /old_locus_tag="p202000562-5__00007"
     CDS             6140..6382
                     /locus_tag="OVO69_RS00040"
                     /old_locus_tag="p202000562-5__00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(6414..7064)
                     /gene="tetR(A)"
                     /locus_tag="OVO69_RS00045"
                     /old_locus_tag="p202000562-5__00008"
     CDS             complement(6414..7064)
                     /gene="tetR(A)"
                     /locus_tag="OVO69_RS00045"
                     /old_locus_tag="p202000562-5__00008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            7170..8369
                     /gene="tet(A)"
                     /locus_tag="OVO69_RS00050"
                     /old_locus_tag="p202000562-5__00009"
     ARG             7171..8366
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             4999..5831
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(2166..2339)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          2263..2868
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cctttccctg ctctgccata agcgcagcga atgccgggta atactcgtca acgatctgat
       61 agagaagggt ttgctcgggt cggtggctct ggtaacgacc agtatcccga tcccggctgg
      121 ccgtcctggc cgccacatga ggcatgttcc gcgtccttgc aatactgtgt ttacatacag
      181 tctatcgctt agcggaaagt tcttttaccc tcagccgaaa tgcctgccgt tgctagacat
      241 tgccagccag tgcccgtcac tcccactctt acattgttcg cagtatctag gtacatgtta
      301 ttgcgtgcac cttcatgatg tataatgtta ttaatgacag cagaaagatc gacagaatta
      361 atattcttcg tacctgggac agttatcggg tgtcggccag tgatgagacc aaaggatgca
      421 gtataagatg aaccactact attatcatgg ccaccattac caccaccgtt aaaactacct
      481 cccgaacttc cagagccgct atgaccatca ttgtaggggt catttggtcc catgtgacca
      541 aaaccaccga cagtaccgct atttaactct gtactcataa aattatcctc tttgactttt
      601 aaaaacagca aaaactgtat ttataacaat gtgtttatat acagtattcg aaatactacg
      661 cccgtcaaaa ccacaccgtc aacaggtttt ttgtgtattt ttcacgcctc acgggcagcc
      721 tcagaccttt tgtgttaaaa cagtaatatg ttctgctgtt acaatacggt acacacagga
      781 gacactgtca cttttatggc atggcacaca gttcaagaga cactgaaatt aacaggaaag
      841 tcgcgctcgc aattatatcg tgacatggct aaaggccttg tcagctatcg cacaggaaaa
      901 aatgatcgcc gggagttcga gaccagtgaa ctgattcggg cctatggcga gctgcgcact
      961 tctgagacac caaaaaggca caaggaggga cactccaaat ctgtcgttag ccaggcggaa
     1021 aatgctcagc tagacgtcat tcagcagaaa ctggacgctc tgcagcagac tgttacccta
     1081 atgcttgaag acaagatggc gcgggaagca gaaaaccgcc tgcgaaatga ggagagggaa
     1141 caactacagg ccgaaattag ccgtctggct gacgcgctgg cacaggaaaa aaagcgggga
     1201 ttctggtcgc ggctgtttcg ctccggttca gagtaacagt gcaggtgact ctgatttacc
     1261 ataacatctg ttatcagcac cgcctgagag gccctcaggg cgtttctgac gggaaaccgg
     1321 taaaacaggc aacaaaccac ccgaaaaccc gccagaaacg cgcaggctct gtttttacgg
     1381 catgcatgag tatgcgcaca agtgcatagc gcggaaattc acaccattca gccggggtaa
     1441 gtcctgtttc cggcgttctt cccatccgaa aacggtgaac ggggacgcaa tttattgcgt
     1501 ggccgtgaag cgttgccggc gcagccggaa aacccgctgt cagcacgttc aggaaggggg
     1561 tgatgtacaa atggcacaga ggatgaaagt gacgtatgag ctgaaggaat gatgtaaaag
     1621 tgatgtataa gtgatgtaaa agtgatgtat gaactgaagg agcgatgtaa ttctgtagtc
     1681 aggaagaata acccgttcat gcaggcatga acgatacagt gaggaacgct ttttttgtca
     1741 cgatctcccc ttaacctgcg taaatcaccc gccatggttg attatttttc aacctcaaaa
     1801 tggtcataca cctaacgcag ccgcgatttt catcgcaact gcatgaatta aaagaaaaaa
     1861 aaggaatatt tcgttttcac gagtaatacc cgggtattaa aaatccactg taattacatg
     1921 aaaataaaga aataaaaggt aataccggag tattaccacg tacgcgggca agcgaaaaag
     1981 ctataacgct tcccggccga caggcgaccc aaaatccaca ggggcatgca ggttctcttc
     2041 agtaacctgc gccattaaat caaaaaggtc atattcgccg ggaagctgcg tatcagtcga
     2101 atccagttgt tcatcatatt cagatggcat ggggtgatat ccagtagctg agaggttgtt
     2161 agcgggtttc ggggcgcagc cctgaaccag tcatgtagcg ctagcggagt gtatactggc
     2221 ttactatgtt gccacgattg cgaaaatcag tgaagtgctt catgtggcag gagaaaaaag
     2281 gcagcaccgg cgcgtcagca gaatatgcga tacaggagat attccgcttc ctcgctcact
     2341 gactcgctac gctcggtcgt tcggctgcgg cgagcggtac cggcttacgg acggggacgg
     2401 aaatttcctg gaagatacca ggaagacact tagcaaagaa gagataaggc cacggcgaag
     2461 ccgtttttcc ataggctccg cccccctgac aagcatcgca aaatctgacg ctcaaatcag
     2521 tggtggcgaa acccgacagg actataaaga tcccaggcgt ttccccctgg tagctccctc
     2581 gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgg
     2641 tttctcattc cacgcctgac actcagttcc gtgaaggcag ttcgctccaa gctggactgt
     2701 atgcacgaac cccccgttca gtccgaccgc tgcgccttgt ccggtaacta tcaacttgag
     2761 tccaacccgg aaagacacga caaaacgcca ctggcagcag ccattagtaa caggagtgca
     2821 cgagaagata gattaagagg tcttgaagta gaggcctgac tgcggctaca ctgaaagaac
     2881 agttttggta atggcggtct cgtgaaccct gttaccgggt taagcagttc cccaactgac
     2941 ttaaccttcg aacaaaccgc ctccccaggc ggttttttcg tttacagagc aagagattac
     3001 gattcattga aaaggatctc aagaagatcc tttgtagatt actatcaatt aatcttccga
     3061 acatgacacc agtcactgtt gattacggtt aaaccgctcc gtaacaggtt tccctcgtat
     3121 tggtgacttg cagcaccaaa gaagcaaaaa agataaatat atctacttat tgagagatga
     3181 tatatttatt tccaaaatgc tacagataca tcatcatgaa acggatcacc gcaaacaggt
     3241 tactcgcctg gggattccct ttcgacccga gcatccgtat gagactcatg ctcgattatt
     3301 attattatag aagcccccat gaataaatcg ctcatcattt tcggcatcgt caacataacc
     3361 tcggacagtt tctccgatgg aggccggtat ctggcgccag acgcagccat tgcgcaggcg
     3421 cgtaagctga tggccgaggg ggcagatgtg atcgacctcg gtccggcatc cagcaatccc
     3481 gacgccgcgc ctgtttcgtc cgacacagaa atcgcgcgta tcgcgccggt gctggacgcg
     3541 ctcaaggcag atggcattcc cgtctcgctc gacagttatc aacccgcgac gcaagcctat
     3601 gccttgtcgc gtggtgtggc ctatctcaat gatattcgcg gttttccaga cgctgcgttc
     3661 tatccgcaat tggcgaaatc atctgccaaa ctcgtcgtta tgcattcggt gcaagacggg
     3721 caggcagatc ggcgcgaggc acccgctggc gacatcatgg atcacattgc ggcgttcttt
     3781 gacgcgcgca tcgcggcgct gacgggtgcc ggtatcaaac gcaaccgcct tgtccttgat
     3841 cccggcatgg ggttttttct gggggctgct cccgaaacct cgctctcggt gctggcgcgg
     3901 ttcgatgaat tgcggctgcg cttcgatttg ccggtgcttc tgtctgtttc gcgcaaatcc
     3961 tttctgcgcg cgctcacagg ccgtggtccg ggggatgtcg gggccgcgac actcgctgca
     4021 gagcttgccg ccgccgcagg tggagctgac ttcatccgca cacacgagcc gcgccccttg
     4081 cgcgacgggc tggcggtatt ggcggcgctg aaagaaaccg caagaattcg ttaactgcac
     4141 attcgggata tttctctata ttcgcgcttc atcagaaaac tgaaggaacc tccattgaat
     4201 cgaactaata ttttttttgg tgaatcgcat tctgactggt tgcctgtcag aggcggagaa
     4261 tctggtgatt ttgtttttcg acgtggtgac gggcatgcct tcgcgaaaat cgcacctgct
     4321 tcccgccgcg gtgagctcgc tggagagcgt gaccgcctca tttggctcaa aggtcgaggt
     4381 gtggcttgcc ccgaggtcat caactggcag gaggaacagg agggtgcatg cttggtgata
     4441 acggcaattc cgggagtacc ggcggctgat ctgtctggag cggatttgct caaagcgtgg
     4501 ccgtcaatgg ggcagcaact tggcgctgtt cacagcctat cggttgatca atgtccgttt
     4561 gagcgcaggc tgtcgcgaat gttcggacgc gccgttgatg tggtgtcccg caatgccgtc
     4621 aatcccgact tcttaccgga cgaggacaag agtacgccgc agctcgatct tttggctcgt
     4681 gtcgaacgag agctaccggt gcggctcgac caagagcgca ccgatatggt tgtttgccat
     4741 ggtgatccct gcatgccgaa cttcatggtg gaccctaaaa ctcttcaatg cacgggtctg
     4801 atcgaccttg ggcggctcgg aacagcagat cgctatgccg atttggcact catgattgct
     4861 aacgccgaag agaactgggc agcgccagat gaagcagagc gcgccttcgc tgtcctattc
     4921 aatgtattgg ggatcgaagc ccccgaccgc gaacgccttg ccttctatct gcgattggac
     4981 cctctgactt ggggttgatg ttcatgccgc ctgtttttcc tgctcattgg cacgtttcgc
     5041 aacctgttct cattgcggac accttttcca gcctcgtttg gaaagtttca ttgccagacg
     5101 ggactcctgc aatcgtcaag ggattgaaac ctataaaaga cattgctgat gaactgcgcg
     5161 gggccgacta tctggtatgg cgcaatggga ggggagcagt ccggttgctc ggtcgtgaga
     5221 acaatctgat gttgctcgaa tatgccgggg agcgaatgct ctctcacatc gttgccgagc
     5281 acggcgacta ccaggcgacc gaaattgcag cggaactaat ggcgaagctg tatgccgcat
     5341 ctgaggaacc cctgccttct gcccttctcc cgatccggga tcgctttgca gctttgtttc
     5401 agcgggcgcg cgatgatcaa aacgcaggtt gtcaaactga ctacgtccac gcggcgatta
     5461 tagccgatca aatgatgagc aatgcctcgg aactgcgtgg gctacatggc gatctgcatc
     5521 atgaaaacat catgttctcc agtcgcggct ggctggtgat agatcccgtc ggtctggtcg
     5581 gtgaagtggg ctttggcgcc gccaatatgt tctacgatcc ggctgacaga gacgaccttt
     5641 gtctcgatcc tagacgcatt gcacagatgg cggacgcatt ctctcgtgcg ctggacgtcg
     5701 atccgcgtcg cctgctcgac caggcgtacg cttatgggtg cctttccgca gcttggaacg
     5761 cggatggaga agaggagcaa cgcgatctag ctatcgcggc cgcgatcaag caggtgcgac
     5821 agacgtcata ctagatatca agcgacttct cctatcccct gggaacacat caatctcacc
     5881 ggagaatatc gctggccaaa gccttagcgt aggattccgc cccttcccgc aaacgacccc
     5941 tggaagcccc gtagcgacgc ggagaggggc gagacaagcc aagggcgcag gctcgatgcg
     6001 cagcacgaca tagccggttc tcgcaaggac gagaatttcc ctgcggtgcc cctcaagtgt
     6061 caatgaaagt ttccaacgcg agccattcgc gagagccttg agtccacgct agatctatct
     6121 catctgcgca aggcagaacg tgaagacggc cgccctggac ctcgcccgcg agcgccaggc
     6181 gcacgaggcc ggcgcgcgga cccgcgccac ggcccacgag cggacgccgc agcaggagcg
     6241 ccagaaggcc gccagagagg ccgagcgcgg ccgtgaggct tggacgctag ggcagggcat
     6301 gaaaaagccc gtagcgggct gctacgggcg tctgacgcgg tggaaagggg gaggggatgt
     6361 tgtctacatg gctctgctgt agtgagtggg ttgcgctccg gcagcggtcc tgatcaatcg
     6421 tcaccctttc tcggtccttc aacgttcctg acaacgagcc tccttttcgc caatccatcg
     6481 acaatcaccg cgagtccctg ctcgaacgct gcgtccggac cggcttcgtc gaaggcgtct
     6541 atcgcggccc gcaacagcgg cgagagcgga gcctgttcaa cggtgccgcc gcgctcgccg
     6601 gcatcgctgt cgccggcctg ctcctcaagc acggccccaa cagtgaagta gctgattgtc
     6661 atcagcgcat tgacggcgtc cccggccgaa aaacccgcct cgcagaggaa gcgaagctgc
     6721 gcgtcggccg tttccatctg cggtgcgccc ggtcgcgtgc cggcatggat gcgcgcgcca
     6781 tcgcggtagg cgagcagcgc ctgcctgaag ctgcgggcat tcccgatcag aaatgagcgc
     6841 cagtcgtcgt cggctctcgg caccgaatgc gtatgattct ccgccagcat ggcttcggcc
     6901 agtgcgtcga gcagcgcccg cttgttcctg aagtgccagt aaagcgccgg ctgctgaacc
     6961 cccaaccgtt ccgccagttt gcgtgtcgtc agaccgtcta cgccgacctc gttcaacagg
     7021 tccagggcgg cacggatcac tgtattcggc tgcaactttg tcatgcttga cactttatca
     7081 ctgataaaca taatatgtcc accaacttat cagtgataaa gaatccgcgc gttcaatcgg
     7141 accagcggag gctggtccgg aggccagacg tgaaacccaa cagacccctg atcgtaattc
     7201 tgagcactgt cgcgctcgac gctgtcggca tcggcctgat tatgccggtg ctgccgggcc
     7261 tcctgcgcga tctggttcac tcgaacgacg tcaccgccca ctatggcatt ctgctggcgc
     7321 tgtatgcgtt gatgcaattt gcctgcgcac ctgtgctggg cgcgctgtcg gatcgtttcg
     7381 ggcggcggcc ggtcttgctc gtctcgctgg ccggcgctgc tgtcgactac gccatcatgg
     7441 cgacggcgcc tttcctttgg gttctctata tcgggcggat cgtggccggc atcaccgggg
     7501 cgactggggc ggtagccggc gcttatattg ccgatatcac tgatggcgat gagcgcgcgc
     7561 ggcacttcgg cttcatgagc gcctgtttcg ggttcgggat ggtcgcggga cctgtgctcg
     7621 gtgggctgat gggcggtttc tccccccacg ctccgttctt cgccgcggca gccttgaacg
     7681 gcctcaattt cctgacgggc tgtttccttt tgccggagtc gcacaaaggc gaacgccggc
     7741 cgttacgccg ggaggctctc aacccgctcg cttcgttccg gtgggcccgg ggcatgaccg
     7801 tcgtcgccgc cctgatggcg gtcttcttca tcatgcaact tgtcggacag gtgccggccg
     7861 cgctttgggt cattttcggc gaggatcgct ttcactggga cgcgaccacg atcggcattt
     7921 cgcttgccgc atttggcatt ctgcattcac tcgcccaggc aatgatcacc ggccctgtag
     7981 ccgcccggct cggcgaaagg cgggcactca tgctcggaat gattgccgac ggcacaggct
     8041 acatcctgct tgccttcgcg acacggggat ggatggcgtt cccgatcatg gtcctgcttg
     8101 cttcgggtgg catcggaatg ccggcgctgc aagcaatgtt gtccaggcag gtggatgagg
     8161 aacgtcaggg gcagctgcaa ggctcactgg cggcgctcac cagcctgacc tcgatcgtcg
     8221 gacccctcct cttcacggcg atctatgcgg cttctataac aacgtggaac gggtgggcat
     8281 ggattgcagg cgctgccctc tacttgctct gcctgccggc gctgcgtcgc gggctttgga
     8341 gaaattcttc aaattcccgt tgcacatagc ccggcaatt
//
