LOCUS       NZ_OP038283             8379 bp    DNA     circular CON 07-JAN-2024
DEFINITION  Shigella sonnei strain 201908234 plasmid p201908234-6, complete
            sequence.
ACCESSION   NZ_OP038283
VERSION     NZ_OP038283.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Recent and rapid emergence of extensively drug-resistant Shigella
            sonnei
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2022) Enteric Bacterial Pathogens Unit, Institut
            Pasteur, 28 rue du Dr Roux, Paris 75724, France
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Sequencing Technology             :: MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/07/2024 04:14:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP038283.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="201908234"
                     /isolation_source="human"
                     /db_xref="taxon:624"
                     /plasmid="p201908234-6"
     gene            join(8337..8379,1..200)
                     /locus_tag="OVO67_RS00005"
     CDS             join(8337..8379,1..200)
                     /locus_tag="OVO67_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(232..882)
                     /gene="tetR(A)"
                     /locus_tag="OVO67_RS00010"
                     /old_locus_tag="p201908234-6__00001"
     CDS             complement(232..882)
                     /gene="tetR(A)"
                     /locus_tag="OVO67_RS00010"
                     /old_locus_tag="p201908234-6__00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            988..2187
                     /gene="tet(A)"
                     /locus_tag="OVO67_RS00015"
                     /old_locus_tag="p201908234-6__00002"
     ARG             989..2184
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<2154..2342)
                     /locus_tag="OVO67_RS00020"
                     /old_locus_tag="p201908234-6__00003"
                     /pseudo=""
     CDS             complement(<2154..2342)
                     /locus_tag="OVO67_RS00020"
                     /old_locus_tag="p201908234-6__00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(2386..2808)
                     /locus_tag="OVO67_RS00025"
                     /old_locus_tag="p201908234-6__00004"
     CDS             complement(2386..2808)
                     /locus_tag="OVO67_RS00025"
                     /old_locus_tag="p201908234-6__00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            2993..3433
                     /locus_tag="OVO67_RS00030"
                     /old_locus_tag="p201908234-6__00005"
     CDS             2993..3433
                     /locus_tag="OVO67_RS00030"
                     /old_locus_tag="p201908234-6__00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(4178..4327)
                     /locus_tag="OVO67_RS00035"
                     /old_locus_tag="p201908234-6__00006"
     CDS             complement(4178..4327)
                     /locus_tag="OVO67_RS00035"
                     /old_locus_tag="p201908234-6__00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            4898..5056
                     /locus_tag="OVO67_RS00055"
     CDS             4898..5056
                     /locus_tag="OVO67_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            5516..6331
                     /gene="sul2"
                     /locus_tag="OVO67_RS00040"
                     /old_locus_tag="p201908234-6__00007"
     ARG             5517..6328
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6392..7195
                     /gene="aph(3'')-Ib"
                     /locus_tag="OVO67_RS00045"
                     /old_locus_tag="p201908234-6__00008"
     ARG             6393..7192
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            7195..8031
                     /locus_tag="OVO67_RS00050"
                     /old_locus_tag="p201908234-6__00009"
     CDS             7195..8031
                     /locus_tag="OVO67_RS00050"
                     /old_locus_tag="p201908234-6__00009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     ARG             7196..8028
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(4363..4536)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          4460..5065
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 acgaggccgg cgcgcggacc cgcgccacgg cccacgagcg gacgccgcag caggagcgcc
       61 agaaggccgc cagagaggcc gagcgcggcc gtgaggcttg gacgctaggg cagggcatga
      121 aaaagcccgt agcgggctgc tacgggcgtc tgacgcggtg gaaaggggga ggggatgttg
      181 tctacatggc tctgctgtag tgagtgggtt gcgctccggc agcggtcctg atcaatcgtc
      241 accctttctc ggtccttcaa cgttcctgac aacgagcctc cttttcgcca atccatcgac
      301 aatcaccgcg agtccctgct cgaacgctgc gtccggaccg gcttcgtcga aggcgtctat
      361 cgcggcccgc aacagcggcg agagcggagc ctgttcaacg gtgccgccgc gctcgccggc
      421 atcgctgtcg ccggcctgct cctcaagcac ggccccaaca gtgaagtagc tgattgtcat
      481 cagcgcattg acggcgtccc cggccgaaaa acccgcctcg cagaggaagc gaagctgcgc
      541 gtcggccgtt tccatctgcg gtgcgcccgg tcgcgtgccg gcatggatgc gcgcgccatc
      601 gcggtaggcg agcagcgcct gcctgaagct gcgggcattc ccgatcagaa atgagcgcca
      661 gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc gccagcatgg cttcggccag
      721 tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa agcgccggct gctgaacccc
      781 caaccgttcc gccagtttgc gtgtcgtcag accgtctacg ccgacctcgt tcaacaggtc
      841 cagggcggca cggatcactg tattcggctg caactttgtc atgcttgaca ctttatcact
      901 gataaacata atatgtccac caacttatca gtgataaaga atccgcgcgt tcaatcggac
      961 cagcggaggc tggtccggag gccagacgtg aaacccaaca gacccctgat cgtaattctg
     1021 agcactgtcg cgctcgacgc tgtcggcatc ggcctgatta tgccggtgct gccgggcctc
     1081 ctgcgcgatc tggttcactc gaacgacgtc accgcccact atggcattct gctggcgctg
     1141 tatgcgttga tgcaatttgc ctgcgcacct gtgctgggcg cgctgtcgga tcgtttcggg
     1201 cggcggccgg tcttgctcgt ctcgctggcc ggcgctgctg tcgactacgc catcatggcg
     1261 acggcgcctt tcctttgggt tctctatatc gggcggatcg tggccggcat caccggggcg
     1321 actggggcgg tagccggcgc ttatattgcc gatatcactg atggcgatga gcgcgcgcgg
     1381 cacttcggct tcatgagcgc ctgtttcggg ttcgggatgg tcgcgggacc tgtgctcggt
     1441 gggctgatgg gcggtttctc cccccacgct ccgttcttcg ccgcggcagc cttgaacggc
     1501 ctcaatttcc tgacgggctg tttccttttg ccggagtcgc acaaaggcga acgccggccg
     1561 ttacgccggg aggctctcaa cccgctcgct tcgttccggt gggcccgggg catgaccgtc
     1621 gtcgccgccc tgatggcggt cttcttcatc atgcaacttg tcggacaggt gccggccgcg
     1681 ctttgggtca ttttcggcga ggatcgcttt cactgggacg cgaccacgat cggcatttcg
     1741 cttgccgcat ttggcattct gcattcactc gcccaggcaa tgatcaccgg ccctgtagcc
     1801 gcccggctcg gcgaaaggcg ggcactcatg ctcggaatga ttgccgacgg cacaggctac
     1861 atcctgcttg ccttcgcgac acggggatgg atggcgttcc cgatcatggt cctgcttgct
     1921 tcgggtggca tcggaatgcc ggcgctgcaa gcaatgttgt ccaggcaggt ggatgaggaa
     1981 cgtcaggggc agctgcaagg ctcactggcg gcgctcacca gcctgacctc gatcgtcgga
     2041 cccctcctct tcacggcgat ctatgcggct tctataacaa cgtggaacgg gtgggcatgg
     2101 attgcaggcg ctgccctcta cttgctctgc ctgccggcgc tgcgtcgcgg gctttggaga
     2161 aattcttcaa attcccgttg cacatagccc ggcaattcct ttccctgctc tgccataagc
     2221 gcagcgaatg ccgggtaata ctcgtcaacg atctgataga gaagggtttg ctcgggtcgg
     2281 tggctctggt aacgaccagt atcccgatcc cggctggccg tcctggccgc cacatgaggc
     2341 atgttccgcg tccttgcaat actgtgttta catacagtct atcgcttagc ggaaagttct
     2401 tttaccctca gccgaaatgc ctgccgttgc tagacattgc cagccagtgc ccgtcactcc
     2461 cactcttaca ttgttcgcag tatctaggta catgttattg cgtgcacctt catgatgtat
     2521 aatgttatta atgacagcag aaagatcgac agaattaata ttcttcgtac ctgggacagt
     2581 tatcgggtgt cggccagtga tgagaccaaa ggatgcagta taagatgaac cactactatt
     2641 atcatggcca ccattaccac caccgttaaa actacctccc gaacttccag agccgctatg
     2701 accatcattg taggggtcat ttggtcccat gtgaccaaaa ccaccgacag taccgctatt
     2761 taactctgta ctcataaaat tatcctcttt gacttttaaa aacagcaaaa actgtattta
     2821 taacaatgtg tttatataca gtattcgaaa tactacgccc gtcaaaacca caccgtcaac
     2881 aggttttttg tgtatttttc acgcctcacg ggcagcctca gaccttttgt gttaaaacag
     2941 taatatgttc tgctgttaca atacggtaca cacaggagac actgtcactt ttatggcatg
     3001 gcacacagtt caagagacac tgaaattaac aggaaagtcg cgctcgcaat tatatcgtga
     3061 catggctaaa ggccttgtca gctatcgcac aggaaaaaat gatcgccggg agttcgagac
     3121 cagtgaactg attcgggcct atggcgagct gcgcacttct gagacaccaa aaaggcacaa
     3181 ggagggacac tccaaatctg tcgttagcca ggcggaaaat gctcagctag acgtcattca
     3241 gcagaaactg gacgctctgc agcagactgt taccctaatg cttgaagaca agatggcgcg
     3301 ggaagcagaa aaccgcctgc gaaatgagga gagggaacaa ctacaggccg aaattagccg
     3361 tctggctgac gcgctggcac aggaaaaaaa gcggggattc tggtcgcggc tgtttcgctc
     3421 cggttcagag taacagtgca ggtgactctg atttaccata acatctgtta tcagcaccgc
     3481 ctgagaggcc ctcagggcgt ttctgacggg aaaccggtaa aacaggcaac aaaccacccg
     3541 aaaacccgcc agaaacgcgc aggctctgtt tttacggcat gcatgagtat gcgcacaagt
     3601 gcatagcgcg gaaattcaca ccattcagcc ggggtaagtc ctgtttccgg cgttcttccc
     3661 atccgaaaac ggtgaacggg gacgcaattt attgcgtggc cgtgaagcgt tgccggcgca
     3721 gccggaaaac ccgctgtcag cacgttcagg aagggggtga tgtacaaatg gcacagagga
     3781 tgaaagtgac gtatgagctg aaggaatgat gtaaaagtga tgtataagtg atgtaaaagt
     3841 gatgtatgaa ctgaaggagc gatgtaattc tgtagtcagg aagaataacc cgttcatgca
     3901 ggcatgaacg atacagtgag gaacgctttt tttgtcacga tctcccctta acctgcgtaa
     3961 atcacccgcc atggttgatt atttttcaac ctcaaaatgg tcatacacct aacgcagccg
     4021 cgattttcat cgcaactgca tgaattaaaa gaaaaaaaag gaatatttcg ttttcacgag
     4081 taatacccgg gtattaaaaa tccactgtaa ttacatgaaa ataaagaaat aaaaggtaat
     4141 accggagtat taccacgtac gcgggcaagc gaaaaagcta taacgcttcc cggccgacag
     4201 gcgacccaaa atccacaggg gcatgcaggt tctcttcagt aacctgcgcc attaaatcaa
     4261 aaaggtcata ttcgccggga agctgcgtat cagtcgaatc cagttgttca tcatattcag
     4321 atggcatggg gtgatatcca gtagctgaga ggttgttagc gggtttcggg gcgcagccct
     4381 gaaccagtca tgtagcgcta gcggagtgta tactggctta ctatgttgcc acgattgcga
     4441 aaatcagtga agtgcttcat gtggcaggag aaaaaaggca gcaccggcgc gtcagcagaa
     4501 tatgcgatac aggagatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg
     4561 gctgcggcga gcggtaccgg cttacggacg gggacggaaa tttcctggaa gataccagga
     4621 agacacttag caaagaagag ataaggccac ggcgaagccg tttttccata ggctccgccc
     4681 ccctgacaag catcgcaaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
     4741 ataaagatcc caggcgtttc cccctggtag ctccctcgtg cgctctcctg ttcctgcctt
     4801 tcggtttacc ggtgtcattc cgctgttatg gccgcggttt ctcattccac gcctgacact
     4861 cagttccgtg aaggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc
     4921 cgaccgctgc gccttgtccg gtaactatca acttgagtcc aacccggaaa gacacgacaa
     4981 aacgccactg gcagcagcca ttagtaacag gagtgcacga gaagatagat taagaggtct
     5041 tgaagtagag gcctgactgc ggctacactg aaagaacagt tttggtaatg gcggtctcgt
     5101 gaaccctgtt accgggttaa gcagttcccc aactgactta accttcgaac aaaccgcctc
     5161 cccaggcggt tttttcgttt acagagcaag agattacgat tcattgaaaa ggatctcaag
     5221 aagatccttt gtagattact atcaattaat cttccgaaca tgacaccagt cactgttgat
     5281 tacggttaaa ccgctccgta acaggtttcc ctcgtattgg tgacttgcag caccaaagaa
     5341 gcaaaaaaga taaatatatc tacttattga gagatgatat atttatttcc aaaatgctac
     5401 agatacatca tcatgaaacg gatcaccgca aacaggttac tcgcctgggg attccctttc
     5461 gacccgagca tccgtatgag actcatgctc gattattatt attatagaag cccccatgaa
     5521 taaatcgctc atcattttcg gcatcgtcaa cataacctcg gacagtttct ccgatggagg
     5581 ccggtatctg gcgccagacg cagccattgc gcaggcgcgt aagctgatgg ccgagggggc
     5641 agatgtgatc gacctcggtc cggcatccag caatcccgac gccgcgcctg tttcgtccga
     5701 cacagaaatc gcgcgtatcg cgccggtgct ggacgcgctc aaggcagatg gcattcccgt
     5761 ctcgctcgac agttatcaac ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta
     5821 tctcaatgat attcgcggtt ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc
     5881 tgccaaactc gtcgttatgc attcggtgca agacgggcag gcagatcggc gcgaggcacc
     5941 cgctggcgac atcatggatc acattgcggc gttctttgac gcgcgcatcg cggcgctgac
     6001 gggtgccggt atcaaacgca accgccttgt ccttgatccc ggcatggggt tttttctggg
     6061 ggctgctccc gaaacctcgc tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt
     6121 cgatttgccg gtgcttctgt ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg
     6181 tggtccgggg gatgtcgggg ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg
     6241 agctgacttc atccgcacac acgagccgcg ccccttgcgc gacgggctgg cggtattggc
     6301 ggcgctgaaa gaaaccgcaa gaattcgtta actgcacatt cgggatattt ctctatattc
     6361 gcgcttcatc agaaaactga aggaacctcc attgaatcga actaatattt tttttggtga
     6421 atcgcattct gactggttgc ctgtcagagg cggagaatct ggtgattttg tttttcgacg
     6481 tggtgacggg catgccttcg cgaaaatcgc acctgcttcc cgccgcggtg agctcgctgg
     6541 agagcgtgac cgcctcattt ggctcaaagg tcgaggtgtg gcttgccccg aggtcatcaa
     6601 ctggcaggag gaacaggagg gtgcatgctt ggtgataacg gcaattccgg gagtaccggc
     6661 ggctgatctg tctggagcgg atttgctcaa agcgtggccg tcaatggggc agcaacttgg
     6721 cgctgttcac agcctatcgg ttgatcaatg tcagtttgag cgcaggctgt cgcgaatgtt
     6781 cggacgcgcc gttgatgtgg tgtcccgcaa tgccgtcaat cccgacttct taccggacga
     6841 ggacaagagt acgccgcagc tcgatctttt ggctcgtgtc gaacgagagc taccggtgcg
     6901 gctcgaccaa gagcgcaccg atatggttgt ttgccatggt gatccctgca tgccgaactt
     6961 catggtggac cctaaaactc ttcaatgcac gggtctgatc gaccttgggc ggctcggaac
     7021 agcagatcgc tatgccgatt tggcactcat gattgctaac gccgaagaga actgggcagc
     7081 gccagatgaa gcagagcgcg ccttcgctgt cctattcaat gtattgggga tcgaagcccc
     7141 cgaccgcgaa cgccttgcct tctatctgcg attggaccct ctgacttggg gttgatgttc
     7201 atgccgcctg tttttcctgc tcattggcac gtttcgcaac ctgttctcat tgcggacacc
     7261 ttttccagcc tcgtttggaa agtttcattg ccagacggga ctcctgcaat cgtcaaggga
     7321 ttgaaaccta tagaagacat tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc
     7381 aatgggaggg gagcagtccg gttgctcggt cgtgagaaca atctgatgtt gctcgaatat
     7441 gccggggagc gaatgctctc tcacatcgtt gccgagcacg gcgactacca ggcgaccgaa
     7501 attgcagcgg aactaatggc gaagctgtat gccgcatctg aggaacccct gccttctgcc
     7561 cttctcccga tccgggatcg ctttgcagct ttgtttcagc gggcgcgcga tgatcaaaac
     7621 gcaggttgtc aaactgacta cgtccacgcg gcgattatag ccgatcaaat gatgagcaat
     7681 gcctcggaac tgcgtgggct acatggcgat ctgcatcatg aaaacatcat gttctccagt
     7741 cgcggctggc tggtgataga tcccgtcggt ctggtcggtg aagtgggctt tggcgccgcc
     7801 aatatgttct acgatccggc tgacagagac gacctttgtc tcgatcctag acgcattgca
     7861 cagatggcgg acgcattctc tcgtgcgctg gacgtcgatc cgcgtcgcct gctcgaccag
     7921 gcgtacgctt atgggtgcct ttccgcagct tggaacgcgg atggagaaga ggagcaacgc
     7981 gatctagcta tcgcggccgc gatcaagcag gtgcgacaga cgtcatacta gatatcaagc
     8041 gacttctcct atcccctggg aacacatcaa tctcaccgga gaatatcgct ggccaaagcc
     8101 ttagcgtagg attccgcccc ttcccgcaaa cgacccctgg aagccccgta gcgacgcgga
     8161 gaggggcgag acaagccaag ggcgcaggct cgatgcgcag cacgacatag ccggttctcg
     8221 caaggacgag aatttccctg cggtgcccct caagtgtcaa tgaaagtttc caacgcgagc
     8281 cattcgcgag agccttgagt ccacgctaga tctatctcat ctgcgcaagg cagaacgtga
     8341 agacggccgc cctggacctc gcccgcgagc gccaggcgc
//
