LOCUS       NZ_OP038280             8401 bp    DNA     circular CON 07-JAN-2024
DEFINITION  Shigella sonnei strain 201908033 plasmid p201908033-4, complete
            sequence.
ACCESSION   NZ_OP038280
VERSION     NZ_OP038280.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Recent and rapid emergence of extensively drug-resistant Shigella
            sonnei
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2022) Enteric Bacterial Pathogens Unit, Institut
            Pasteur, 28 rue du Dr Roux, Paris 75724, France
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Sequencing Technology             :: MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/07/2024 04:06:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP038280.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="201908033"
                     /isolation_source="human"
                     /db_xref="taxon:624"
                     /plasmid="p201908033-4"
     gene            510..668
                     /locus_tag="OVP25_RS00055"
     CDS             510..668
                     /locus_tag="OVP25_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            1128..1943
                     /gene="sul2"
                     /locus_tag="OVP25_RS00005"
                     /old_locus_tag="p201908033-4__00001"
     ARG             1129..1940
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2004..2807
                     /gene="aph(3'')-Ib"
                     /locus_tag="OVP25_RS00010"
                     /old_locus_tag="p201908033-4__00002"
     ARG             2005..2804
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            2807..3643
                     /locus_tag="OVP25_RS00015"
                     /old_locus_tag="p201908033-4__00003"
     CDS             2807..3643
                     /locus_tag="OVP25_RS00015"
                     /old_locus_tag="p201908033-4__00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            3949..4191
                     /locus_tag="OVP25_RS00020"
                     /old_locus_tag="p201908033-4__00004"
     CDS             3949..4191
                     /locus_tag="OVP25_RS00020"
                     /old_locus_tag="p201908033-4__00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(4223..4873)
                     /gene="tetR(A)"
                     /locus_tag="OVP25_RS00025"
                     /old_locus_tag="p201908033-4__00005"
     CDS             complement(4223..4873)
                     /gene="tetR(A)"
                     /locus_tag="OVP25_RS00025"
                     /old_locus_tag="p201908033-4__00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            4979..6178
                     /gene="tet(A)"
                     /locus_tag="OVP25_RS00030"
                     /old_locus_tag="p201908033-4__00006"
     ARG             4980..6175
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<6145..6333)
                     /locus_tag="OVP25_RS00035"
                     /old_locus_tag="p201908033-4__00007"
                     /pseudo=""
     CDS             complement(<6145..6333)
                     /locus_tag="OVP25_RS00035"
                     /old_locus_tag="p201908033-4__00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(6377..6799)
                     /locus_tag="OVP25_RS00040"
                     /old_locus_tag="p201908033-4__00008"
     CDS             complement(6377..6799)
                     /locus_tag="OVP25_RS00040"
                     /old_locus_tag="p201908033-4__00008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            6984..7424
                     /locus_tag="OVP25_RS00045"
                     /old_locus_tag="p201908033-4__00009"
     CDS             6984..7424
                     /locus_tag="OVP25_RS00045"
                     /old_locus_tag="p201908033-4__00009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(8191..8340)
                     /locus_tag="OVP25_RS00050"
                     /old_locus_tag="p201908033-4__00010"
     CDS             complement(8191..8340)
                     /locus_tag="OVP25_RS00050"
                     /old_locus_tag="p201908033-4__00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     ARG             2808..3640
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(2..148)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.324"
                     /coverage="85.0"
                     /evalue="1.22e-69"
     Typing          72..677
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 catgtagcgc tagcggagtg tatactggct tactatgttg ccacgattgc gaaaatcagt
       61 gaagtgcttc atgtggcagg agaaaaaagg cagcaccggc gcgtcagcag aatatgcgat
      121 acaggagata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cggctgcggc
      181 gagcggtacc ggcttacgga cggggacgga aatttcctgg aagataccag gaagacactt
      241 agcaaagaag agataaggcc acggcgaagc cgtttttcca taggctccgc ccccctgaca
      301 agcatcgcaa aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat
      361 cccaggcgtt tccccctggt agctccctcg tgcgctctcc tgttcctgcc tttcggttta
      421 ccggtgtcat tccgctgtta tggccgcggt ttctcattcc acgcctgaca ctcagttccg
      481 tgaaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct
      541 gcgccttgtc cggtaactat caacttgagt ccaacccgga aagacacgac aaaacgccac
      601 tggcagcagc cattagtaac aggagtgcac gagaagatag attaagaggt cttgaagtag
      661 aggcctgact gcggctacac tgaaagaaca gttttggtaa tggcggtctc gtgaaccctg
      721 ttaccgggtt aagcagttcc ccaactgact taaccttcga acaaaccgcc tccccaggcg
      781 gttttttcgt ttacagagca agagattacg attcattgaa aaggatctca agaagatcct
      841 ttgtagatta ctatcaatta atcttccgaa catgacacca gtcactgttg attacggtta
      901 aaccgctccg taacaggttt ccctcgtatt ggtgacttgc agcaccaaag aagcaaaaaa
      961 gataaatata tctacttatt gagagatgat atatttattt ccaaaatgct acagatacat
     1021 catcatgaaa cggatcaccg caaacaggtt actcgcctgg ggattccctt tcgacccgag
     1081 catccgtatg agactcatgc tcgattatta ttattataga agcccccatg aataaatcgc
     1141 tcatcatttt cggcatcgtc aacataacct cggacagttt ctccgatgga ggccggtatc
     1201 tggcgccaga cgcagccatt gcgcaggcgc gtaagctgat ggccgagggg gcagatgtga
     1261 tcgacctcgg tccggcatcc agcaatcccg acgccgcgcc tgtttcgtcc gacacagaaa
     1321 tcgcgcgtat cgcgccggtg ctggacgcgc tcaaggcaga tggcattccc gtctcgctcg
     1381 acagttatca acccgcgacg caagcctatg ccttgtcgcg tggtgtggcc tatctcaatg
     1441 atattcgcgg ttttccagac gctgcgttct atccgcaatt ggcgaaatca tctgccaaac
     1501 tcgtcgttat gcattcggtg caagacgggc aggcagatcg gcgcgaggca cccgctggcg
     1561 acatcatgga tcacattgcg gcgttctttg acgcgcgcat cgcggcgctg acgggtgccg
     1621 gtatcaaacg caaccgcctt gtccttgatc ccggcatggg gttttttctg ggggctgctc
     1681 ccgaaacctc gctctcggtg ctggcgcggt tcgatgaatt gcggctgcgc ttcgatttgc
     1741 cggtgcttct gtctgtttcg cgcaaatcct ttctgcgcgc gctcacaggc cgtggtccgg
     1801 gggatgtcgg ggccgcgaca ctcgctgcag agcttgccgc cgccgcaggt ggagctgact
     1861 tcatccgcac acacgagccg cgccccttgc gcgacgggct ggcggtattg gcggcgctga
     1921 aagaaaccgc aagaattcgt taactgcaca ttcgggatat ttctctatat tcgcgcttca
     1981 tcagaaaact gaaggaacct ccattgaatc gaactaatat tttttttggt gaatcgcatt
     2041 ctgactggtt gcctgtcaga ggcggagaat ctggtgattt tgtttttcga cgtggtgacg
     2101 ggcatgcctt cgcgaaaatc gcacctgctt cccgccgcgg tgagctcgct ggagagcgtg
     2161 accgcctcat ttggctcaaa ggtcgaggtg tggcttgccc cgaggtcatc aactggcagg
     2221 aggaacagga gggtgcatgc ttggtgataa cggcaattcc gggagtaccg gcggctgatc
     2281 tgtctggagc ggatttgctc aaagcgtggc cgtcaatggg gcagcaactt ggcgctgttc
     2341 acagcctatc ggttgatcaa tgtcagtttg agcgcaggct gtcgcgaatg ttcggacgcg
     2401 ccgttgatgt ggtgtcccgc aatgccgtca atcccgactt cttaccggac gaggacaaga
     2461 gtacgccgca gctcgatctt ttggctcgtg tcgaacgaga gctaccggtg cggctcgacc
     2521 aagagcgcac cgatatggtt gtttgccatg gtgatccctg catgccgaac ttcatggtgg
     2581 accctaaaac tcttcaatgc acgggtctga tcgaccttgg gcggctcgga acagcagatc
     2641 gctatgccga tttggcactc atgattgcta acgccgaaga gaactgggca gcgccagatg
     2701 aagcagagcg cgccttcgct gtcctattca atgtattggg gatcgaagcc cccgaccgcg
     2761 aacgccttgc cttctatctg cgattggacc ctctgacttg gggttgatgt tcatgccgcc
     2821 tgtttttcct gctcattggc acgtttcgca acctgttctc attgcggaca ccttttccag
     2881 cctcgtttgg aaagtttcat tgccagacgg gactcctgca atcgtcaagg gattgaaacc
     2941 tatagaagac attgctgatg aactgcgcgg ggccgactat ctggtatggc gcaatgggag
     3001 gggagcagtc cggttgctcg gtcgtgagaa caatctgatg ttgctcgaat atgccgggga
     3061 gcgaatgctc tctcacatcg ttgccgagca cggcgactac caggcgaccg aaattgcagc
     3121 ggaactaatg gcgaagctgt atgccgcatc tgaggaaccc ctgccttctg cccttctccc
     3181 gatccgggat cgctttgcag ctttgtttca gcgggcgcgc gatgatcaaa acgcaggttg
     3241 tcaaactgac tacgtccacg cggcgattat agccgatcaa atgatgagca atgcctcgga
     3301 actgcgtggg ctacatggcg atctgcatca tgaaaacatc atgttctcca gtcgcggctg
     3361 gctggtgata gatcccgtcg gtctggtcgg tgaagtgggc tttggcgccg ccaatatgtt
     3421 ctacgatccg gctgacagag acgacctttg tctcgatcct agacgcattg cacagatggc
     3481 ggacgcattc tctcgtgcgc tggacgtcga tccgcgtcgc ctgctcgacc aggcgtacgc
     3541 ttatgggtgc ctttccgcag cttggaacgc ggatggagaa gaggagcaac gcgatctagc
     3601 tatcgcggcc gcgatcaagc aggtgcgaca gacgtcatac tagatatcaa gcgacttctc
     3661 ctatcccctg ggaacacatc aatctcaccg gagaatatcg ctggccaaag ccttagcgta
     3721 ggattccgcc ccttcccgca aacgacccct ggaagccccg tagcgacgcg gagaggggcg
     3781 agacaagcca agggcgcagg ctcgatgcgc agcacgacat agccggttct cgcaaggacg
     3841 agaatttccc tgcggtgccc ctcaagtgtc aatgaaagtt tccaacgcga gccattcgcg
     3901 agagccttga gtccacgcta gatctatctc atctgcgcaa ggcagaacgt gaagacggcc
     3961 gccctggacc tcgcccgcga gcgccaggcg cacgaggccg gcgcgcggac ccgcgccacg
     4021 gcccacgagc ggacgccgca gcaggagcgc cagaaggccg ccagagaggc cgagcgcggc
     4081 cgtgaggctt ggacgctagg gcagggcatg aaaaagcccg tagcgggctg ctacgggcgt
     4141 ctgacgcggt ggaaaggggg aggggatgtt gtctacatgg ctctgctgta gtgagtgggt
     4201 tgcgctccgg cagcggtcct gatcaatcgt caccctttct cggtccttca acgttcctga
     4261 caacgagcct ccttttcgcc aatccatcga caatcaccgc gagtccctgc tcgaacgctg
     4321 cgtccggacc ggcttcgtcg aaggcgtcta tcgcggcccg caacagcggc gagagcggag
     4381 cctgttcaac ggtgccgccg cgctcgccgg catcgctgtc gccggcctgc tcctcaagca
     4441 cggccccaac agtgaagtag ctgattgtca tcagcgcatt gacggcgtcc ccggccgaaa
     4501 aacccgcctc gcagaggaag cgaagctgcg cgtcggccgt ttccatctgc ggtgcgcccg
     4561 gtcgcgtgcc ggcatggatg cgcgcgccat cgcggtaggc gagcagcgcc tgcctgaagc
     4621 tgcgggcatt cccgatcaga aatgagcgcc agtcgtcgtc ggctctcggc accgaatgcg
     4681 tatgattctc cgccagcatg gcttcggcca gtgcgtcgag cagcgcccgc ttgttcctga
     4741 agtgccagta aagcgccggc tgctgaaccc ccaaccgttc cgccagtttg cgtgtcgtca
     4801 gaccgtctac gccgacctcg ttcaacaggt ccagggcggc acggatcact gtattcggct
     4861 gcaactttgt catgcttgac actttatcac tgataaacat aatatgtcca ccaacttatc
     4921 agtgataaag aatccgcgcg ttcaatcgga ccagcggagg ctggtccgga ggccagacgt
     4981 gaaacccaac agacccctga tcgtaattct gagcactgtc gcgctcgacg ctgtcggcat
     5041 cggcctgatt atgccggtgc tgccgggcct cctgcgcgat ctggttcact cgaacgacgt
     5101 caccgcccac tatggcattc tgctggcgct gtatgcgttg atgcaatttg cctgcgcacc
     5161 tgtgctgggc gcgctgtcgg atcgtttcgg gcggcggccg gtcttgctcg tctcgctggc
     5221 cggcgctgct gtcgactacg ccatcatggc gacggcgcct ttcctttggg ttctctatat
     5281 cgggcggatc gtggccggca tcaccggggc gactggggcg gtagccggcg cttatattgc
     5341 cgatatcact gatggcgatg agcgcgcgcg gcacttcggc ttcatgagcg cctgtttcgg
     5401 gttcgggatg gtcgcgggac ctgtgctcgg tgggctgatg ggcggtttct ccccccacgc
     5461 tccgttcttc gccgcggcag ccttgaacgg cctcaatttc ctgacgggct gtttcctttt
     5521 gccggagtcg cacaaaggcg aacgccggcc gttacgccgg gaggctctca acccgctcgc
     5581 ttcgttccgg tgggcccggg gcatgaccgt cgtcgccgcc ctgatggcgg tcttcttcat
     5641 catgcaactt gtcggacagg tgccggccgc gctttgggtc attttcggcg aggatcgctt
     5701 tcactgggac gcgaccacga tcggcatttc gcttgccgca tttggcattc tgcattcact
     5761 cgcccaggca atgatcaccg gccctgtagc cgcccggctc ggcgaaaggc gggcactcat
     5821 gctcggaatg attgccgacg gcacaggcta catcctgctt gccttcgcga cacggggatg
     5881 gatggcgttc ccgatcatgg tcctgcttgc ttcgggtggc atcggaatgc cggcgctgca
     5941 agcaatgttg tccaggcagg tggatgagga acgtcagggg cagctgcaag gctcactggc
     6001 ggcgctcacc agcctgacct cgatcgtcgg acccctcctc ttcacggcga tctatgcggc
     6061 ttctataaca acgtggaacg ggtgggcatg gattgcaggc gctgccctct acttgctctg
     6121 cctgccggcg ctgcgtcgcg ggctttggag aaattcttca aattcccgtt gcacatagcc
     6181 cggcaattcc tttccctgct ctgccataag cgcagcgaat gccgggtaat actcgtcaac
     6241 gatctgatag agaagggttt gctcgggtcg gtggctctgg taacgaccag tatcccgatc
     6301 ccggctggcc gtcctggccg ccacatgagg catgttccgc gtccttgcaa tactgtgttt
     6361 acatacagtc tatcgcttag cggaaagttc ttttaccctc agccgaaatg cctgccgttg
     6421 ctagacattg ccagccagtg cccgtcactc ccactcttac attgttcgca gtatctaggt
     6481 acatgttatt gcgtgcacct tcatgatgta taatgttatt aatgacagca gaaagatcga
     6541 cagaattaat attcttcgta cctgggacag ttatcgggtg tcggccagtg atgagaccaa
     6601 aggatgcagt ataagatgaa ccactactat tatcatggcc accattacca ccaccgttaa
     6661 aactacctcc cgaacttcca gagccgctat gaccatcatt gtaggggtca tttggtccca
     6721 tgtgaccaaa accaccgaca gtaccgctat ttaactctgt actcataaaa ttatcctctt
     6781 tgacttttaa aaacagcaaa aactgtattt ataacaatgt gtttatatac agtattcgaa
     6841 atactacgcc cgtcaaaacc acaccgtcaa caggtttttt gtgtattttt cacgcctcac
     6901 gggcagcctc agaccttttg tgttaaaaca gtaatatgtt ctgctgttac aatacggtac
     6961 acacaggaga cactgtcact tttatggcat ggcacacagt tcaagagaca ctgaaattaa
     7021 caggaaagtc gcgctcgcaa ttatatcgtg acatggctaa aggccttgtc agctatcgca
     7081 caggaaaaaa tgatcgccgg gagttcgaga ccagtgaact gattcgggcc tatggcgagc
     7141 tgcgcacttc tgagacacca aaaaggcaca aggagggaca ctccaaatct gtcgttagcc
     7201 aggcggaaaa tgctcagcta gacgtcattc agcagaaact ggacgctctg cagcagactg
     7261 ttaccctaat gcttgaagac aagatggcgc gggaagcaga aaaccgcctg cgaaatgagg
     7321 agagggaaca actacaggcc gaaattagcc gtctggctga cgcgctggca caggaaaaaa
     7381 agcggggatt ctggtcgcgg ctgtttcgct ccggttcaga gtaacagtgc aggtgactct
     7441 gatttaccat aacatctgtt atcagcaccg cctgagaggc cctcagggcg tttctgacgg
     7501 gaaaccggta aaacaggcaa caaaccaccc gaaaacccgc cagaaacgcg caggctctgt
     7561 ttttacggca tgcatgagta tgcgcacaag tgcatagcgc ggaaattcac accattcagc
     7621 cggggtaagt cctgtttccg gcgttcttcc catccgaaaa cggtgaacgg ggacgcaatt
     7681 tattgcgtgg ccgtgaagcg ttgccggcgc agccggaaaa cccgctgtca gcacgttcag
     7741 gaagggggtg atgtacaaat ggcacagagg atgaaagtga cgtatgagct gaaggaatga
     7801 tgtaaaagtg atgtataagt gatgtaaaag tgatgtataa gtgatgtaaa agtgatgtat
     7861 gaactgaagg agcgatgtaa ttctgtagtc aggaagaata acccgttcat gcaggcatga
     7921 acgatacagt gaggaacgct ttttttgtca cgatctcccc ttaacctgcg taaatcaccc
     7981 gccatggttg attatttttc aacctcaaaa tggtcataca cctaacgcag ccgcgatttt
     8041 catcgcaact gcatgaatta aaagaaaaaa aaggaatatt tcgttttcac gagtaatacc
     8101 cgggtattaa aaatccactg taattacatg aaaataaaga aataaaaggt aataccggag
     8161 tattaccacg tacgcgggca agcgaaaaag ctataacgct tcccggccga caggcgaccc
     8221 aaaatccaca ggggcatgca ggttctcttc agtaacctgc gccattaaat caaaaaggtc
     8281 atattcgccg ggaagctgcg tatcagtcga atccagttgt tcatcatatt cagatggcat
     8341 ggggtgatat ccagtagctg agaggttgtt agcgggtttc ggggcgcagc cctgaaccag
     8401 t
//
