LOCUS       NZ_OP038273             8401 bp    DNA     circular CON 07-JAN-2024
DEFINITION  Shigella sonnei strain 201702782 plasmid p201702782-3, complete
            sequence.
ACCESSION   NZ_OP038273
VERSION     NZ_OP038273.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Recent and rapid emergence of extensively drug-resistant Shigella
            sonnei
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2022) Enteric Bacterial Pathogens Unit, Institut
            Pasteur, 28 rue du Dr Roux, Paris 75724, France
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Sequencing Technology             :: MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/07/2024 04:10:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP038273.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="201702782"
                     /isolation_source="human"
                     /db_xref="taxon:624"
                     /plasmid="p201702782-3"
     gene            2..151
                     /locus_tag="OVO62_RS00005"
                     /old_locus_tag="p201702782-3__00001"
     CDS             2..151
                     /locus_tag="OVO62_RS00005"
                     /old_locus_tag="p201702782-3__00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(918..1358)
                     /locus_tag="OVO62_RS00010"
                     /old_locus_tag="p201702782-3__00002"
     CDS             complement(918..1358)
                     /locus_tag="OVO62_RS00010"
                     /old_locus_tag="p201702782-3__00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            1543..1965
                     /locus_tag="OVO62_RS00015"
                     /old_locus_tag="p201702782-3__00003"
     CDS             1543..1965
                     /locus_tag="OVO62_RS00015"
                     /old_locus_tag="p201702782-3__00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            2009..>2197
                     /locus_tag="OVO62_RS00020"
                     /old_locus_tag="p201702782-3__00004"
                     /pseudo=""
     CDS             2009..>2197
                     /locus_tag="OVO62_RS00020"
                     /old_locus_tag="p201702782-3__00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(2164..3363)
                     /gene="tet(A)"
                     /locus_tag="OVO62_RS00025"
                     /old_locus_tag="p201702782-3__00005"
     ARG             complement(2168..3363)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3469..4119
                     /gene="tetR(A)"
                     /locus_tag="OVO62_RS00030"
                     /old_locus_tag="p201702782-3__00006"
     CDS             3469..4119
                     /gene="tetR(A)"
                     /locus_tag="OVO62_RS00030"
                     /old_locus_tag="p201702782-3__00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            complement(4151..4393)
                     /locus_tag="OVO62_RS00035"
                     /old_locus_tag="p201702782-3__00007"
     CDS             complement(4151..4393)
                     /locus_tag="OVO62_RS00035"
                     /old_locus_tag="p201702782-3__00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(4699..5535)
                     /locus_tag="OVO62_RS00040"
                     /old_locus_tag="p201702782-3__00008"
     CDS             complement(4699..5535)
                     /locus_tag="OVO62_RS00040"
                     /old_locus_tag="p201702782-3__00008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(5535..6338)
                     /gene="aph(3'')-Ib"
                     /locus_tag="OVO62_RS00045"
                     /old_locus_tag="p201702782-3__00009"
     ARG             complement(5539..6338)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            complement(6399..7214)
                     /gene="sul2"
                     /locus_tag="OVO62_RS00050"
                     /old_locus_tag="p201702782-3__00010"
     ARG             complement(6403..7214)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(7674..7832)
                     /locus_tag="OVO62_RS00055"
     CDS             complement(7674..7832)
                     /locus_tag="OVO62_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     ARG             complement(4703..5535)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          8195..8368
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(7666..8271)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 catgccatct gaatatgatg aacaactgga ttcgactgat acgcagcttc ccggcgaata
       61 tgaccttttt gatttaatgg cgcaggttac tgaagagaac ctgcatgccc ctgtggattt
      121 tgggtcgcct gtcggccggg aagcgttata gctttttcgc ttgcccgcgt acgtggtaat
      181 actccggtat taccttttat ttctttattt tcatgtaatt acagtggatt tttaataccc
      241 gggtattact cgtgaaaacg aaatattcct tttttttctt ttaattcatg cagttgcgat
      301 gaaaatcgcg gctgcgttag gtgtatgacc attttgaggt tgaaaaataa tcaaccatgg
      361 cgggtgattt acgcaggtta aggggagatc gtgacaaaaa aagcgttcct cactgtatcg
      421 ttcatgcctg catgaacggg ttattcttcc tgactacaga attacatcgc tccttcagtt
      481 catacatcac ttttacatca cttatacatc acttttacat cacttataca tcacttttac
      541 atcattcctt cagctcatac gtcactttca tcctctgtgc catttgtaca tcaccccctt
      601 cctgaacgtg ctgacagcgg gttttccggc tgcgccggca acgcttcacg gccacgcaat
      661 aaattgcgtc cccgttcacc gttttcggat gggaagaacg ccggaaacag gacttacccc
      721 ggctgaatgg tgtgaatttc cgcgctatgc acttgtgcgc atactcatgc atgccgtaaa
      781 aacagagcct gcgcgtttct ggcgggtttt cgggtggttt gttgcctgtt ttaccggttt
      841 cccgtcagaa acgccctgag ggcctctcag gcggtgctga taacagatgt tatggtaaat
      901 cagagtcacc tgcactgtta ctctgaaccg gagcgaaaca gccgcgacca gaatccccgc
      961 tttttttcct gtgccagcgc gtcagccaga cggctaattt cggcctgtag ttgttccctc
     1021 tcctcatttc gcaggcggtt ttctgcttcc cgcgccatct tgtcttcaag cattagggta
     1081 acagtctgct gcagagcgtc cagtttctgc tgaatgacgt ctagctgagc attttccgcc
     1141 tggctaacga cagatttgga gtgtccctcc ttgtgccttt ttggtgtctc agaagtgcgc
     1201 agctcgccat aggcccgaat cagttcactg gtctcgaact cccggcgatc attttttcct
     1261 gtgcgatagc tgacaaggcc tttagccatg tcacgatata attgcgagcg cgactttcct
     1321 gttaatttca gtgtctcttg aactgtgtgc catgccataa aagtgacagt gtctcctgtg
     1381 tgtaccgtat tgtaacagca gaacatatta ctgttttaac acaaaaggtc tgaggctgcc
     1441 cgtgaggcgt gaaaaataca caaaaaacct gttgacggtg tggttttgac gggcgtagta
     1501 tttcgaatac tgtatataaa cacattgtta taaatacagt ttttgctgtt tttaaaagtc
     1561 aaagaggata attttatgag tacagagtta aatagcggta ctgtcggtgg ttttggtcac
     1621 atgggaccaa atgaccccta caatgatggt catagcggct ctggaagttc gggaggtagt
     1681 tttaacggtg gtggtaatgg tggccatgat aatagtagtg gttcatctta tactgcatcc
     1741 tttggtctca tcactggccg acacccgata actgtcccag gtacgaagaa tattaattct
     1801 gtcgatcttt ctgctgtcat taataacatt atacatcatg aaggtgcacg caataacatg
     1861 tacctagata ctgcgaacaa tgtaagagtg ggagtgacgg gcactggctg gcaatgtcta
     1921 gcaacggcag gcatttcggc tgagggtaaa agaactttcc gctaagcgat agactgtatg
     1981 taaacacagt attgcaagga cgcggaacat gcctcatgtg gcggccagga cggccagccg
     2041 ggatcgggat actggtcgtt accagagcca ccgacccgag caaacccttc tctatcagat
     2101 cgttgacgag tattacccgg cattcgctgc gcttatggca gagcagggaa aggaattgcc
     2161 gggctatgtg caacgggaat ttgaagaatt tctccaaagc ccgcgacgca gcgccggcag
     2221 gcagagcaag tagagggcag cgcctgcaat ccatgcccac ccgttccacg ttgttataga
     2281 agccgcatag atcgccgtga agaggagggg tccgacgatc gaggtcaggc tggtgagcgc
     2341 cgccagtgag ccttgcagct gcccctgacg ttcctcatcc acctgcctgg acaacattgc
     2401 ttgcagcgcc ggcattccga tgccacccga agcaagcagg accatgatcg ggaacgccat
     2461 ccatccccgt gtcgcgaagg caagcaggat gtagcctgtg ccgtcggcaa tcattccgag
     2521 catgagtgcc cgcctttcgc cgagccgggc ggctacaggg ccggtgatca ttgcctgggc
     2581 gagtgaatgc agaatgccaa atgcggcaag cgaaatgccg atcgtggtcg cgtcccagtg
     2641 aaagcgatcc tcgccgaaaa tgacccaaag cgcggccggc acctgtccga caagttgcat
     2701 gatgaagaag accgccatca gggcggcgac gacggtcatg ccccgggccc accggaacga
     2761 agcgagcggg ttgagagcct cccggcgtaa cggccggcgt tcgcctttgt gcgactccgg
     2821 caaaaggaaa cagcccgtca ggaaattgag gccgttcaag gctgccgcgg cgaagaacgg
     2881 agcgtggggg gagaaaccgc ccatcagccc accgagcaca ggtcccgcga ccatcccgaa
     2941 cccgaaacag gcgctcatga agccgaagtg ccgcgcgcgc tcatcgccat cagtgatatc
     3001 ggcaatataa gcgccggcta ccgccccagt cgccccggtg atgccggcca cgatccgccc
     3061 gatatagaga acccaaagga aaggcgccgt cgccatgatg gcgtagtcga cagcagcgcc
     3121 ggccagcgag acgagcaaga ccggccgccg cccgaaacga tccgacagcg cgcccagcac
     3181 aggtgcgcag gcaaattgca tcaacgcata cagcgccagc agaatgccat agtgggcggt
     3241 gacgtcgttc gagtgaacca gatcgcgcag gaggcccggc agcaccggca taatcaggcc
     3301 gatgccgaca gcgtcgagcg cgacagtgct cagaattacg atcaggggtc tgttgggttt
     3361 cacgtctggc ctccggacca gcctccgctg gtccgattga acgcgcggat tctttatcac
     3421 tgataagttg gtggacatat tatgtttatc agtgataaag tgtcaagcat gacaaagttg
     3481 cagccgaata cagtgatccg tgccgccctg gacctgttga acgaggtcgg cgtagacggt
     3541 ctgacgacac gcaaactggc ggaacggttg ggggttcagc agccggcgct ttactggcac
     3601 ttcaggaaca agcgggcgct gctcgacgca ctggccgaag ccatgctggc ggagaatcat
     3661 acgcattcgg tgccgagagc cgacgacgac tggcgctcat ttctgatcgg gaatgcccgc
     3721 agcttcaggc aggcgctgct cgcctaccgc gatggcgcgc gcatccatgc cggcacgcga
     3781 ccgggcgcac cgcagatgga aacggccgac gcgcagcttc gcttcctctg cgaggcgggt
     3841 ttttcggccg gggacgccgt caatgcgctg atgacaatca gctacttcac tgttggggcc
     3901 gtgcttgagg agcaggccgg cgacagcgat gccggcgagc gcggcggcac cgttgaacag
     3961 gctccgctct cgccgctgtt gcgggccgcg atagacgcct tcgacgaagc cggtccggac
     4021 gcagcgttcg agcagggact cgcggtgatt gtcgatggat tggcgaaaag gaggctcgtt
     4081 gtcaggaacg ttgaaggacc gagaaagggt gacgattgat caggaccgct gccggagcgc
     4141 aacccactca ctacagcaga gccatgtaga caacatcccc tccccctttc caccgcgtca
     4201 gacgcccgta gcagcccgct acgggctttt tcatgccctg ccctagcgtc caagcctcac
     4261 ggccgcgctc ggcctctctg gcggccttct ggcgctcctg ctgcggcgtc cgctcgtggg
     4321 ccgtggcgcg ggtccgcgcg ccggcctcgt gcgcctggcg ctcgcgggcg aggtccaggg
     4381 cggccgtctt cacgttctgc cttgcgcaga tgagatagat ctagcgtgga ctcaaggctc
     4441 tcgcgaatgg ctcgcgttgg aaactttcat tgacacttga ggggcaccgc agggaaattc
     4501 tcgtccttgc gagaaccggc tatgtcgtgc tgcgcatcga gcctgcgccc ttggcttgtc
     4561 tcgcccctct ccgcgtcgct acggggcttc caggggtcgt ttgcgggaag gggcggaatc
     4621 ctacgctaag gctttggcca gcgatattct ccggtgagat tgatgtgttc ccaggggata
     4681 ggagaagtcg cttgatatct agtatgacgt ctgtcgcacc tgcttgatcg cggccgcgat
     4741 agctagatcg cgttgctcct cttctccatc cgcgttccaa gctgcggaaa ggcacccata
     4801 agcgtacgcc tggtcgagca ggcgacgcgg atcgacgtcc agcgcacgag agaatgcgtc
     4861 cgccatctgt gcaatgcgtc taggatcgag acaaaggtcg tctctgtcag ccggatcgta
     4921 gaacatattg gcggcgccaa agcccacttc accgaccaga ccgacgggat ctatcaccag
     4981 ccagccgcga ctggagaaca tgatgttttc atgatgcaga tcgccatgta gcccacgcag
     5041 ttccgaggca ttgctcatca tttgatcggc tataatcgcc gcgtggacgt agtcagtttg
     5101 acaacctgcg ttttgatcat cgcgcgcccg ctgaaacaaa gctgcaaagc gatcccggat
     5161 cgggagaagg gcagaaggca ggggttcctc agatgcggca tacagcttcg ccattagttc
     5221 cgctgcaatt tcggtcgcct ggtagtcgcc gtgctcggca acgatgtgag agagcattcg
     5281 ctccccggca tattcgagca acatcagatt gttctcacga ccgagcaacc ggactgctcc
     5341 cctcccattg cgccatacca gatagtcggc cccgcgcagt tcatcagcaa tgtcttctat
     5401 aggtttcaat cccttgacga ttgcaggagt cccgtctggc aatgaaactt tccaaacgag
     5461 gctggaaaag gtgtccgcaa tgagaacagg ttgcgaaacg tgccaatgag caggaaaaac
     5521 aggcggcatg aacatcaacc ccaagtcaga gggtccaatc gcagatagaa ggcaaggcgt
     5581 tcgcggtcgg gggcttcgat ccccaataca ttgaatagga cagcgaaggc gcgctctgct
     5641 tcatctggcg ctgcccagtt ctcttcggcg ttagcaatca tgagtgccaa atcggcatag
     5701 cgatctgctg ttccgagccg cccaaggtcg atcagacccg tgcattgaag agttttaggg
     5761 tccaccatga agttcggcat gcagggatca ccatggcaaa caaccatatc ggtgcgctct
     5821 tggtcgagcc gcaccggtag ctctcgttcg acacgagcca aaagatcgag ctgcggcgta
     5881 ctcttgtcct cgtccggtaa gaagtcggga ttgacggcat tgcgggacac cacatcaacg
     5941 gcgcgtccga acattcgcga cagcctgcgc tcaaactgac attgatcaac cgataggctg
     6001 tgaacagcgc caagttgctg ccccattgac ggccacgctt tgagcaaatc cgctccagac
     6061 agatcagccg ccggtactcc cggaattgcc gttatcacca agcatgcacc ctcctgttcc
     6121 tcctgccagt tgatgacctc ggggcaagcc acacctcgac ctttgagcca aatgaggcgg
     6181 tcacgctctc cagcgagctc accgcggcgg gaagcaggtg cgattttcgc gaaggcatgc
     6241 ccgtcaccac gtcgaaaaac aaaatcacca gattctccgc ctctgacagg caaccagtca
     6301 gaatgcgatt caccaaaaaa aatattagtt cgattcaatg gaggttcctt cagttttctg
     6361 atgaagcgcg aatatagaga aatatcccga atgtgcagtt aacgaattct tgcggtttct
     6421 ttcagcgccg ccaataccgc cagcccgtcg cgcaaggggc gcggctcgtg tgtgcggatg
     6481 aagtcagctc cacctgcggc ggcggcaagc tctgcagcga gtgtcgcggc cccgacatcc
     6541 cccggaccac ggcctgtgag cgcgcgcaga aaggatttgc gcgaaacaga cagaagcacc
     6601 ggcaaatcga agcgcagccg caattcatcg aaccgcgcca gcaccgagag cgaggtttcg
     6661 ggagcagccc ccagaaaaaa ccccatgccg ggatcaagga caaggcggtt gcgtttgata
     6721 ccggcacccg tcagcgccgc gatgcgcgcg tcaaagaacg ccgcaatgtg atccatgatg
     6781 tcgccagcgg gtgcctcgcg ccgatctgcc tgcccgtctt gcaccgaatg cataacgacg
     6841 agtttggcag atgatttcgc caattgcgga tagaacgcag cgtctggaaa accgcgaata
     6901 tcattgagat aggccacacc acgcgacaag gcataggctt gcgtcgcggg ttgataactg
     6961 tcgagcgaga cgggaatgcc atctgccttg agcgcgtcca gcaccggcgc gatacgcgcg
     7021 atttctgtgt cggacgaaac aggcgcggcg tcgggattgc tggatgccgg accgaggtcg
     7081 atcacatctg ccccctcggc catcagctta cgcgcctgcg caatggctgc gtctggcgcc
     7141 agataccggc ctccatcgga gaaactgtcc gaggttatgt tgacgatgcc gaaaatgatg
     7201 agcgatttat tcatgggggc ttctataata ataataatcg agcatgagtc tcatacggat
     7261 gctcgggtcg aaagggaatc cccaggcgag taacctgttt gcggtgatcc gtttcatgat
     7321 gatgtatctg tagcattttg gaaataaata tatcatctct caataagtag atatatttat
     7381 cttttttgct tctttggtgc tgcaagtcac caatacgagg gaaacctgtt acggagcggt
     7441 ttaaccgtaa tcaacagtga ctggtgtcat gttcggaaga ttaattgata gtaatctaca
     7501 aaggatcttc ttgagatcct tttcaatgaa tcgtaatctc ttgctctgta aacgaaaaaa
     7561 ccgcctgggg aggcggtttg ttcgaaggtt aagtcagttg gggaactgct taacccggta
     7621 acagggttca cgagaccgcc attaccaaaa ctgttctttc agtgtagccg cagtcaggcc
     7681 tctacttcaa gacctcttaa tctatcttct cgtgcactcc tgttactaat ggctgctgcc
     7741 agtggcgttt tgtcgtgtct ttccgggttg gactcaagtt gatagttacc ggacaaggcg
     7801 cagcggtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgccttc
     7861 acggaactga gtgtcaggcg tggaatgaga aaccgcggcc ataacagcgg aatgacaccg
     7921 gtaaaccgaa aggcaggaac aggagagcgc acgagggagc taccaggggg aaacgcctgg
     7981 gatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat tttgcgatgc
     8041 ttgtcagggg ggcggagcct atggaaaaac ggcttcgccg tggccttatc tcttctttgc
     8101 taagtgtctt cctggtatct tccaggaaat ttccgtcccc gtccgtaagc cggtaccgct
     8161 cgccgcagcc gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga atatctcctg
     8221 tatcgcatat tctgctgacg cgccggtgct gccttttttc tcctgccaca tgaagcactt
     8281 cactgatttt cgcaatcgtg gcaacatagt aagccagtat acactccgct agcgctacat
     8341 gactggttca gggctgcgcc ccgaaacccg ctaacaacct ctcagctact ggatatcacc
     8401 c
//
