LOCUS       NZ_OP038270             8401 bp    DNA     circular CON 07-JAN-2024
DEFINITION  Shigella sonnei strain 201603898 plasmid p201603898-4, complete
            sequence.
ACCESSION   NZ_OP038270
VERSION     NZ_OP038270.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Recent and rapid emergence of extensively drug-resistant Shigella
            sonnei
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2022) Enteric Bacterial Pathogens Unit, Institut
            Pasteur, 28 rue du Dr Roux, Paris 75724, France
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Sequencing Technology             :: MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/07/2024 04:07:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP038270.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="201603898"
                     /isolation_source="human"
                     /db_xref="taxon:624"
                     /plasmid="p201603898-4"
     gene            complement(join(8292..8401,1..49))
                     /locus_tag="OVO60_RS00005"
     CDS             complement(join(8292..8401,1..49))
                     /locus_tag="OVO60_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            620..769
                     /locus_tag="OVO60_RS00010"
                     /old_locus_tag="p201603898-4__00001"
     CDS             620..769
                     /locus_tag="OVO60_RS00010"
                     /old_locus_tag="p201603898-4__00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(1536..1976)
                     /locus_tag="OVO60_RS00015"
                     /old_locus_tag="p201603898-4__00002"
     CDS             complement(1536..1976)
                     /locus_tag="OVO60_RS00015"
                     /old_locus_tag="p201603898-4__00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            2161..2583
                     /locus_tag="OVO60_RS00020"
                     /old_locus_tag="p201603898-4__00003"
     CDS             2161..2583
                     /locus_tag="OVO60_RS00020"
                     /old_locus_tag="p201603898-4__00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            2627..>2815
                     /locus_tag="OVO60_RS00025"
                     /old_locus_tag="p201603898-4__00004"
                     /pseudo=""
     CDS             2627..>2815
                     /locus_tag="OVO60_RS00025"
                     /old_locus_tag="p201603898-4__00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(2782..3981)
                     /gene="tet(A)"
                     /locus_tag="OVO60_RS00030"
                     /old_locus_tag="p201603898-4__00005"
     ARG             complement(2786..3981)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4087..4737
                     /gene="tetR(A)"
                     /locus_tag="OVO60_RS00035"
                     /old_locus_tag="p201603898-4__00006"
     CDS             4087..4737
                     /gene="tetR(A)"
                     /locus_tag="OVO60_RS00035"
                     /old_locus_tag="p201603898-4__00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            complement(4769..5011)
                     /locus_tag="OVO60_RS00040"
                     /old_locus_tag="p201603898-4__00007"
     CDS             complement(4769..5011)
                     /locus_tag="OVO60_RS00040"
                     /old_locus_tag="p201603898-4__00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(5317..6153)
                     /locus_tag="OVO60_RS00045"
                     /old_locus_tag="p201603898-4__00008"
     CDS             complement(5317..6153)
                     /locus_tag="OVO60_RS00045"
                     /old_locus_tag="p201603898-4__00008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(6153..6956)
                     /gene="aph(3'')-Ib"
                     /locus_tag="OVO60_RS00050"
                     /old_locus_tag="p201603898-4__00009"
     ARG             complement(6157..6956)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            complement(7017..7832)
                     /gene="sul2"
                     /locus_tag="OVO60_RS00055"
                     /old_locus_tag="p201603898-4__00010"
     ARG             complement(7021..7832)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(5321..6153)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          412..585
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(2..488)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.65"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 agttaccgga caaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct
       61 tggagcgaac tgccttcacg gaactgagtg tcaggcgtgg aatgagaaac cgcggccata
      121 acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagctac
      181 cagggggaaa cgcctgggat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc
      241 gtcagatttt gcgatgcttg tcaggggggc ggagcctatg gaaaaacggc ttcgccgtgg
      301 ccttatctct tctttgctaa gtgtcttcct ggtatcttcc aggaaatttc cgtccccgtc
      361 cgtaagccgg taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg
      421 aagcggaata tctcctgtat cgcatattct gctgacgcgc cggtgctgcc ttttttctcc
      481 tgccacatga agcacttcac tgattttcgc aatcgtggca acatagtaag ccagtataca
      541 ctccgctagc gctacatgac tggttcaggg ctgcgccccg aaacccgcta acaacctctc
      601 agctactgga tatcacccca tgccatctga atatgatgaa caactggatt cgactgatac
      661 gcagcttccc ggcgaatatg acctttttga tttaatggcg caggttactg aagagaacct
      721 gcatgcccct gtggattttg ggtcgcctgt cggccgggaa gcgttatagc tttttcgctt
      781 gcccgcgtac gtggtaatac tccggtatta ccttttattt ctttattttc atgtaattac
      841 agtggatttt taatacccgg gtattactcg tgaaaacgaa atattccttt tttttctttt
      901 aattcatgca gttgcgatga aaatcgcggc tgcgttaggt gtatgaccat tttgaggttg
      961 aaaaataatc aaccatggcg ggtgatttac gcaggttaag gggagatcgt gacaaaaaaa
     1021 gcgttcctca ctgtatcgtt catgcctgca tgaacgggtt attcttcctg actacagaat
     1081 tacatcgctc cttcagttca tacatcactt ttacatcact tatacatcac ttttacatca
     1141 cttatacatc acttttacat cattccttca gctcatacgt cactttcatc ctctgtgcca
     1201 tttgtacatc acccccttcc tgaacgtgct gacagcgggt tttccggctg cgccggcaac
     1261 gcttcacggc cacgcaataa attgcgtccc cgttcaccgt tttcggatgg gaagaacgcc
     1321 ggaaacagga cttaccccgg ctgaatggtg tgaatttccg cgctatgcac ttgtgcgcat
     1381 actcatgcat gccgtaaaaa cagagcctgc gcgtttctgg cgggttttcg ggtggtttgt
     1441 tgcctgtttt accggtttcc cgtcagaaac gccctgaggg cctctcaggc ggtgctgata
     1501 acagatgtta tggtaaatca gagtcacctg cactgttact ctgaaccgga gcgaaacagc
     1561 cgcgaccaga atccccgctt tttttcctgt gccagcgcgt cagccagacg gctaatttcg
     1621 gcctgtagtt gttccctctc ctcatttcgc aggcggtttt ctgcttcccg cgccatcttg
     1681 tcttcaagca ttagggtaac agtctgctgc agagcgtcca gtttctgctg aatgacgtct
     1741 agctgagcat tttccgcctg gctaacgaca gatttggagt gtccctcctt gtgccttttt
     1801 ggtgtctcag aagtgcgcag ctcgccatag gcccgaatca gttcactggt ctcgaactcc
     1861 cggcgatcat tttttcctgt gcgatagctg acaaggcctt tagccatgtc acgatataat
     1921 tgcgagcgcg actttcctgt taatttcagt gtctcttgaa ctgtgtgcca tgccataaaa
     1981 gtgacagtgt ctcctgtgtg taccgtattg taacagcaga acatattact gttttaacac
     2041 aaaaggtctg aggctgcccg tgaggcgtga aaaatacaca aaaaacctgt tgacggtgtg
     2101 gttttgacgg gcgtagtatt tcgaatactg tatataaaca cattgttata aatacagttt
     2161 ttgctgtttt taaaagtcaa agaggataat tttatgagta cagagttaaa tagcggtact
     2221 gtcggtggtt ttggtcacat gggaccaaat gacccctaca atgatggtca tagcggctct
     2281 ggaagttcgg gaggtagttt taacggtggt ggtaatggtg gccatgataa tagtagtggt
     2341 tcatcttata ctgcatcctt tggtctcatc actggccgac acccgataac tgtcccaggt
     2401 acgaagaata ttaattctgt cgatctttct gctgtcatta ataacattat acatcatgaa
     2461 ggtgcacgca ataacatgta cctagatact gcgaacaatg taagagtggg agtgacgggc
     2521 actggctggc aatgtctagc aacggcaggc atttcggctg agggtaaaag aactttccgc
     2581 taagcgatag actgtatgta aacacagtat tgcaaggacg cggaacatgc ctcatgtggc
     2641 ggccaggacg gccagccggg atcgggatac tggtcgttac cagagccacc gacccgagca
     2701 aacccttctc tatcagatcg ttgacgagta ttacccggca ttcgctgcgc ttatggcaga
     2761 gcagggaaag gaattgccgg gctatgtgca acgggaattt gaagaatttc tccaaagccc
     2821 gcgacgcagc gccggcaggc agagcaagta gagggcagcg cctgcaatcc atgcccaccc
     2881 gttccacgtt gttatagaag ccgcatagat cgccgtgaag aggaggggtc cgacgatcga
     2941 ggtcaggctg gtgagcgccg ccagtgagcc ttgcagctgc ccctgacgtt cctcatccac
     3001 ctgcctggac aacattgctt gcagcgccgg cattccgatg ccacccgaag caagcaggac
     3061 catgatcggg aacgccatcc atccccgtgt cgcgaaggca agcaggatgt agcctgtgcc
     3121 gtcggcaatc attccgagca tgagtgcccg cctttcgccg agccgggcgg ctacagggcc
     3181 ggtgatcatt gcctgggcga gtgaatgcag aatgccaaat gcggcaagcg aaatgccgat
     3241 cgtggtcgcg tcccagtgaa agcgatcctc gccgaaaatg acccaaagcg cggccggcac
     3301 ctgtccgaca agttgcatga tgaagaagac cgccatcagg gcggcgacga cggtcatgcc
     3361 ccgggcccac cggaacgaag cgagcgggtt gagagcctcc cggcgtaacg gccggcgttc
     3421 gcctttgtgc gactccggca aaaggaaaca gcccgtcagg aaattgaggc cgttcaaggc
     3481 tgccgcggcg aagaacggag cgtgggggga gaaaccgccc atcagcccac cgagcacagg
     3541 tcccgcgacc atcccgaacc cgaaacaggc gctcatgaag ccgaagtgcc gcgcgcgctc
     3601 atcgccatca gtgatatcgg caatataagc gccggctacc gccccagtcg ccccggtgat
     3661 gccggccacg atccgcccga tatagagaac ccaaaggaaa ggcgccgtcg ccatgatggc
     3721 gtagtcgaca gcagcgccgg ccagcgagac gagcaagacc ggccgccgcc cgaaacgatc
     3781 cgacagcgcg cccagcacag gtgcgcaggc aaattgcatc aacgcataca gcgccagcag
     3841 aatgccatag tgggcggtga cgtcgttcga gtgaaccaga tcgcgcagga ggcccggcag
     3901 caccggcata atcaggccga tgccgacagc gtcgagcgcg acagtgctca gaattacgat
     3961 caggggtctg ttgggtttca cgtctggcct ccggaccagc ctccgctggt ccgattgaac
     4021 gcgcggattc tttatcactg ataagttggt ggacatatta tgtttatcag tgataaagtg
     4081 tcaagcatga caaagttgca gccgaataca gtgatccgtg ccgccctgga cctgttgaac
     4141 gaggtcggcg tagacggtct gacgacacgc aaactggcgg aacggttggg ggttcagcag
     4201 ccggcgcttt actggcactt caggaacaag cgggcgctgc tcgacgcact ggccgaagcc
     4261 atgctggcgg agaatcatac gcattcggtg ccgagagccg acgacgactg gcgctcattt
     4321 ctgatcggga atgcccgcag cttcaggcag gcgctgctcg cctaccgcga tggcgcgcgc
     4381 atccatgccg gcacgcgacc gggcgcaccg cagatggaaa cggccgacgc gcagcttcgc
     4441 ttcctctgcg aggcgggttt ttcggccggg gacgccgtca atgcgctgat gacaatcagc
     4501 tacttcactg ttggggccgt gcttgaggag caggccggcg acagcgatgc cggcgagcgc
     4561 ggcggcaccg ttgaacaggc tccgctctcg ccgctgttgc gggccgcgat agacgccttc
     4621 gacgaagccg gtccggacgc agcgttcgag cagggactcg cggtgattgt cgatggattg
     4681 gcgaaaagga ggctcgttgt caggaacgtt gaaggaccga gaaagggtga cgattgatca
     4741 ggaccgctgc cggagcgcaa cccactcact acagcagagc catgtagaca acatcccctc
     4801 cccctttcca ccgcgtcaga cgcccgtagc agcccgctac gggctttttc atgccctgcc
     4861 ctagcgtcca agcctcacgg ccgcgctcgg cctctctggc ggccttctgg cgctcctgct
     4921 gcggcgtccg ctcgtgggcc gtggcgcggg tccgcgcgcc ggcctcgtgc gcctggcgct
     4981 cgcgggcgag gtccagggcg gccgtcttca cgttctgcct tgcgcagatg agatagatct
     5041 agcgtggact caaggctctc gcgaatggct cgcgttggaa actttcattg acacttgagg
     5101 ggcaccgcag ggaaattctc gtccttgcga gaaccggcta tgtcgtgctg cgcatcgagc
     5161 ctgcgccctt ggcttgtctc gcccctctcc gcgtcgctac ggggcttcca ggggtcgttt
     5221 gcgggaaggg gcggaatcct acgctaaggc tttggccagc gatattctcc ggtgagattg
     5281 atgtgttccc aggggatagg agaagtcgct tgatatctag tatgacgtct gtcgcacctg
     5341 cttgatcgcg gccgcgatag ctagatcgcg ttgctcctct tctccatccg cgttccaagc
     5401 tgcggaaagg cacccataag cgtacgcctg gtcgagcagg cgacgcggat cgacgtccag
     5461 cgcacgagag aatgcgtccg ccatctgtgc aatgcgtcta ggatcgagac aaaggtcgtc
     5521 tctgtcagcc ggatcgtaga acatattggc ggcgccaaag cccacttcac cgaccagacc
     5581 gacgggatct atcaccagcc agccgcgact ggagaacatg atgttttcat gatgcagatc
     5641 gccatgtagc ccacgcagtt ccgaggcatt gctcatcatt tgatcggcta taatcgccgc
     5701 gtggacgtag tcagtttgac aacctgcgtt ttgatcatcg cgcgcccgct gaaacaaagc
     5761 tgcaaagcga tcccggatcg ggagaagggc agaaggcagg ggttcctcag atgcggcata
     5821 cagcttcgcc attagttccg ctgcaatttc ggtcgcctgg tagtcgccgt gctcggcaac
     5881 gatgtgagag agcattcgct ccccggcata ttcgagcaac atcagattgt tctcacgacc
     5941 gagcaaccgg actgctcccc tcccattgcg ccataccaga tagtcggccc cgcgcagttc
     6001 atcagcaatg tcttctatag gtttcaatcc cttgacgatt gcaggagtcc cgtctggcaa
     6061 tgaaactttc caaacgaggc tggaaaaggt gtccgcaatg agaacaggtt gcgaaacgtg
     6121 ccaatgagca ggaaaaacag gcggcatgaa catcaacccc aagtcagagg gtccaatcgc
     6181 agatagaagg caaggcgttc gcggtcgggg gcttcgatcc ccaatacatt gaataggaca
     6241 gcgaaggcgc gctctgcttc atctggcgct gcccagttct cttcggcgtt agcaatcatg
     6301 agtgccaaat cggcatagcg atctgctgtt ccgagccgcc caaggtcgat cagacccgtg
     6361 cattgaagag ttttagggtc caccatgaag ttcggcatgc agggatcacc atggcaaaca
     6421 accatatcgg tgcgctcttg gtcgagccgc accggtagct ctcgttcgac acgagccaaa
     6481 agatcgagct gcggcgtact cttgtcctcg tccggtaaga agtcgggatt gacggcattg
     6541 cgggacacca catcaacggc gcgtccgaac attcgcgaca gcctgcgctc aaactgacat
     6601 tgatcaaccg ataggctgtg aacagcgcca agttgctgcc ccattgacgg ccacgctttg
     6661 agcaaatccg ctccagacag atcagccgcc ggtactcccg gaattgccgt tatcaccaag
     6721 catgcaccct cctgttcctc ctgccagttg atgacctcgg ggcaagccac acctcgacct
     6781 ttgagccaaa tgaggcggtc acgctctcca gcgagctcac cgcggcggga agcaggtgcg
     6841 attttcgcga aggcatgccc gtcaccacgt cgaaaaacaa aatcaccaga ttctccgcct
     6901 ctgacaggca accagtcaga atgcgattca ccaaaaaaaa tattagttcg attcaatgga
     6961 ggttccttca gttttctgat gaagcgcgaa tatagagaaa tatcccgaat gtgcagttaa
     7021 cgaattcttg cggtttcttt cagcgccgcc aataccgcca gcccgtcgcg caaggggcgc
     7081 ggctcgtgtg tgcggatgaa gtcagctcca cctgcggcgg cggcaagctc tgcagcgagt
     7141 gtcgcggccc cgacatcccc cggaccacgg cctgtgagcg cgcgcagaaa ggatttgcgc
     7201 gaaacagaca gaagcaccgg caaatcgaag cgcagccgca attcatcgaa ccgcgccagc
     7261 accgagagcg aggtttcggg agcagccccc agaaaaaacc ccatgccggg atcaaggaca
     7321 aggcggttgc gtttgatacc ggcacccgtc agcgccgcga tgcgcgcgtc aaagaacgcc
     7381 gcaatgtgat ccatgatgtc gccagcgggt gcctcgcgcc gatctgcctg cccgtcttgc
     7441 accgaatgca taacgacgag tttggcagat gatttcgcca attgcggata gaacgcagcg
     7501 tctggaaaac cgcgaatatc attgagatag gccacaccac gcgacaaggc ataggcttgc
     7561 gtcgcgggtt gataactgtc gagcgagacg ggaatgccat ctgccttgag cgcgtccagc
     7621 accggcgcga tacgcgcgat ttctgtgtcg gacgaaacag gcgcggcgtc gggattgctg
     7681 gatgccggac cgaggtcgat cacatctgcc ccctcggcca tcagcttacg cgcctgcgca
     7741 atggctgcgt ctggcgccag ataccggcct ccatcggaga aactgtccga ggttatgttg
     7801 acgatgccga aaatgatgag cgatttattc atgggggctt ctataataat aataatcgag
     7861 catgagtctc atacggatgc tcgggtcgaa agggaatccc caggcgagta acctgtttgc
     7921 ggtgatccgt ttcatgatga tgtatctgta gcattttgga aataaatata tcatctctca
     7981 ataagtagat atatttatct tttttgcttc tttggtgctg caagtcacca atacgaggga
     8041 aacctgttac ggagcggttt aaccgtaatc aacagtgact ggtgtcatgt tcggaagatt
     8101 aattgatagt aatctacaaa ggatcttctt gagatccttt tcaatgaatc gtaatctctt
     8161 gctctgtaaa cgaaaaaacc gcctggggag gcggtttgtt cgaaggttaa gtcagttggg
     8221 gaactgctta acccggtaac agggttcacg agaccgccat taccaaaact gttctttcag
     8281 tgtagccgca gtcaggcctc tacttcaaga cctcttaatc tatcttctcg tgcactcctg
     8341 ttactaatgg ctgctgccag tggcgttttg tcgtgtcttt ccgggttgga ctcaagttga
     8401 t
//
