LOCUS       NZ_OP038268             8401 bp    DNA     circular CON 07-JAN-2024
DEFINITION  Shigella sonnei strain 201502874 plasmid p201502874-5, complete
            sequence.
ACCESSION   NZ_OP038268
VERSION     NZ_OP038268.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Recent and rapid emergence of extensively drug-resistant Shigella
            sonnei
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Lefevre,S., Njamkepo,E., Ruckly,C., Carle,I., Lejay-Collin,M.,
            Fabre,L., Maria Pardos de la Gandara,M. and Weill,F.-X.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2022) Enteric Bacterial Pathogens Unit, Institut
            Pasteur, 28 rue du Dr Roux, Paris 75724, France
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Sequencing Technology             :: MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/07/2024 04:13:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP038268.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="201502874"
                     /isolation_source="human"
                     /db_xref="taxon:624"
                     /plasmid="p201502874-5"
     gene            complement(join(8082..8401,1..517))
                     /locus_tag="OVO61_RS00005"
     CDS             complement(join(8082..8401,1..517))
                     /locus_tag="OVO61_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(517..1320)
                     /gene="aph(3'')-Ib"
                     /locus_tag="OVO61_RS00010"
                     /old_locus_tag="p201502874-5__00001"
     ARG             complement(521..1320)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            complement(1381..2196)
                     /gene="sul2"
                     /locus_tag="OVO61_RS00015"
                     /old_locus_tag="p201502874-5__00002"
     ARG             complement(1385..2196)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(2656..2814)
                     /locus_tag="OVO61_RS00055"
     CDS             complement(2656..2814)
                     /locus_tag="OVO61_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            3385..3534
                     /locus_tag="OVO61_RS00020"
                     /old_locus_tag="p201502874-5__00003"
     CDS             3385..3534
                     /locus_tag="OVO61_RS00020"
                     /old_locus_tag="p201502874-5__00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(4301..4741)
                     /locus_tag="OVO61_RS00025"
                     /old_locus_tag="p201502874-5__00004"
     CDS             complement(4301..4741)
                     /locus_tag="OVO61_RS00025"
                     /old_locus_tag="p201502874-5__00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            4926..5348
                     /locus_tag="OVO61_RS00030"
                     /old_locus_tag="p201502874-5__00005"
     CDS             4926..5348
                     /locus_tag="OVO61_RS00030"
                     /old_locus_tag="p201502874-5__00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            5392..>5580
                     /locus_tag="OVO61_RS00035"
                     /old_locus_tag="p201502874-5__00006"
                     /pseudo=""
     CDS             5392..>5580
                     /locus_tag="OVO61_RS00035"
                     /old_locus_tag="p201502874-5__00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(5547..6746)
                     /gene="tet(A)"
                     /locus_tag="OVO61_RS00040"
                     /old_locus_tag="p201502874-5__00007"
     ARG             complement(5551..6746)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6852..7502
                     /gene="tetR(A)"
                     /locus_tag="OVO61_RS00045"
                     /old_locus_tag="p201502874-5__00008"
     CDS             6852..7502
                     /gene="tetR(A)"
                     /locus_tag="OVO61_RS00045"
                     /old_locus_tag="p201502874-5__00008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            complement(7534..7776)
                     /locus_tag="OVO61_RS00050"
                     /old_locus_tag="p201502874-5__00009"
     CDS             complement(7534..7776)
                     /locus_tag="OVO61_RS00050"
                     /old_locus_tag="p201502874-5__00009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     ARG             complement(8083..8399)
                     /name="APH(6)-Id"
                     /gene="APH(6)-Id"
                     /gene_name="APH(6)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.05"
                     /coverage="37.77"
     ARG             complement(3..517)
                     /name="APH(6)-Id"
                     /gene="APH(6)-Id"
                     /gene_name="APH(6)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="61.87"
     Typing          3177..3350
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(2648..3253)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gatcgccatg tagcccacgc agttccgagg cattgctcat catttgatcg gctataatcg
       61 ccgcgtggac gtagtcagtt tgacaacctg cgttttgatc atcgcgcgcc cgctgaaaca
      121 aagctgcaaa gcgatcccgg atcgggagaa gggcagaagg caggggttcc tcagatgcgg
      181 catacagctt cgccattagt tccgctgcaa tttcggtcgc ctggtagtcg ccgtgctcgg
      241 caacgatgtg agagagcatt cgctccccgg catattcgag caacatcaga ttgttctcac
      301 gaccgagcaa ccggactgct cccctcccat tgcgccatac cagatagtcg gccccgcgca
      361 gttcatcagc aatgtcttct ataggtttca atcccttgac gattgcagga gtcccgtctg
      421 gcaatgaaac tttccaaacg aggctggaaa aggtgtccgc aatgagaaca ggttgcgaaa
      481 cgtgccaatg agcaggaaaa acaggcggca tgaacatcaa ccccaagtca gagggtccaa
      541 tcgcagatag aaggcaaggc gttcgcggtc gggggcttcg atccccaata cattgaatag
      601 gacagcgaag gcgcgctctg cttcatctgg cgctgcccag ttctcttcgg cgttagcaat
      661 catgagtgcc aaatcggcat agcgatctgc tgttccgagc cgcccaaggt cgatcagacc
      721 cgtgcattga agagttttag ggtccaccat gaagttcggc atgcagggat caccatggca
      781 aacaaccata tcggtgcgct cttggtcgag ccgcaccggt agctctcgtt cgacacgagc
      841 caaaagatcg agctgcggcg tactcttgtc ctcgtccggt aagaagtcgg gattgacggc
      901 attgcgggac accacatcaa cggcgcgtcc gaacattcgc gacagcctgc gctcaaactg
      961 acattgatca accgataggc tgtgaacagc gccaagttgc tgccccattg acggccacgc
     1021 tttgagcaaa tccgctccag acagatcagc cgccggtact cccggaattg ccgttatcac
     1081 caagcatgca ccctcctgtt cctcctgcca gttgatgacc tcggggcaag ccacacctcg
     1141 acctttgagc caaatgaggc ggtcacgctc tccagcgagc tcaccgcggc gggaagcagg
     1201 tgcgattttc gcgaaggcat gcccgtcacc acgtcgaaaa acaaaatcac cagattctcc
     1261 gcctctgaca ggcaaccagt cagaatgcga ttcaccaaaa aaaatattag ttcgattcaa
     1321 tggaggttcc ttcagttttc tgatgaagcg cgaatataga gaaatatccc gaatgtgcag
     1381 ttaacgaatt cttgcggttt ctttcagcgc cgccaatacc gccagcccgt cgcgcaaggg
     1441 gcgcggctcg tgtgtgcgga tgaagtcagc tccacctgcg gcggcggcaa gctctgcagc
     1501 gagtgtcgcg gccccgacat cccccggacc acggcctgtg agcgcgcgca gaaaggattt
     1561 gcgcgaaaca gacagaagca ccggcaaatc gaagcgcagc cgcaattcat cgaaccgcgc
     1621 cagcaccgag agcgaggttt cgggagcagc ccccagaaaa aaccccatgc cgggatcaag
     1681 gacaaggcgg ttgcgtttga taccggcacc cgtcagcgcc gcgatgcgcg cgtcaaagaa
     1741 cgccgcaatg tgatccatga tgtcgccagc gggtgcctcg cgccgatctg cctgcccgtc
     1801 ttgcaccgaa tgcataacga cgagtttggc agatgatttc gccaattgcg gatagaacgc
     1861 agcgtctgga aaaccgcgaa tatcattgag ataggccaca ccacgcgaca aggcataggc
     1921 ttgcgtcgcg ggttgataac tgtcgagcga gacgggaatg ccatctgcct tgagcgcgtc
     1981 cagcaccggc gcgatacgcg cgatttctgt gtcggacgaa acaggcgcgg cgtcgggatt
     2041 gctggatgcc ggaccgaggt cgatcacatc tgccccctcg gccatcagct tacgcgcctg
     2101 cgcaatggct gcgtctggcg ccagataccg gcctccatcg gagaaactgt ccgaggttat
     2161 gttgacgatg ccgaaaatga tgagcgattt attcatgggg gcttctataa taataataat
     2221 cgagcatgag tctcatacgg atgctcgggt cgaaagggaa tccccaggcg agtaacctgt
     2281 ttgcggtgat ccgtttcatg atgatgtatc tgtagcattt tggaaataaa tatatcatct
     2341 ctcaataagt agatatattt atcttttttg cttctttggt gctgcaagtc accaatacga
     2401 gggaaacctg ttacggagcg gtttaaccgt aatcaacagt gactggtgtc atgttcggaa
     2461 gattaattga tagtaatcta caaaggatct tcttgagatc cttttcaatg aatcgtaatc
     2521 tcttgctctg taaacgaaaa aaccgcctgg ggaggcggtt tgttcgaagg ttaagtcagt
     2581 tggggaactg cttaacccgg taacagggtt cacgagaccg ccattaccaa aactgttctt
     2641 tcagtgtagc cgcagtcagg cctctacttc aagacctctt aatctatctt ctcgtgcact
     2701 cctgttacta atggctgctg ccagtggcgt tttgtcgtgt ctttccgggt tggactcaag
     2761 ttgatagtta ccggacaagg cgcagcggtc ggactgaacg gggggttcgt gcatacagtc
     2821 cagcttggag cgaactgcct tcacggaact gagtgtcagg cgtggaatga gaaaccgcgg
     2881 ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga
     2941 gctaccaggg ggaaacgcct gggatcttta tagtcctgtc gggtttcgcc accactgatt
     3001 tgagcgtcag attttgcgat gcttgtcagg ggggcggagc ctatggaaaa acggcttcgc
     3061 cgtggcctta tctcttcttt gctaagtgtc ttcctggtat cttccaggaa atttccgtcc
     3121 ccgtccgtaa gccggtaccg ctcgccgcag ccgaacgacc gagcgtagcg agtcagtgag
     3181 cgaggaagcg gaatatctcc tgtatcgcat attctgctga cgcgccggtg ctgccttttt
     3241 tctcctgcca catgaagcac ttcactgatt ttcgcaatcg tggcaacata gtaagccagt
     3301 atacactccg ctagcgctac atgactggtt cagggctgcg ccccgaaacc cgctaacaac
     3361 ctctcagcta ctggatatca ccccatgcca tctgaatatg atgaacaact ggattcgact
     3421 gatacgcagc ttcccggcga atatgacctt tttgatttaa tggcgcaggt tactgaagag
     3481 aacctgcatg cccctgtgga ttttgggtcg cctgtcggcc gggaagcgtt atagcttttt
     3541 cgcttgcccg cgtacgtggt aatactccgg tattaccttt tatttcttta ttttcatgta
     3601 attacagtgg atttttaata cccgggtatt actcgtgaaa acgaaatatt cctttttttt
     3661 cttttaattc atgcagttgc gatgaaaatc gcggctgcgt taggtgtatg accattttga
     3721 ggttgaaaaa taatcaacca tggcgggtga tttacgcagg ttaaggggag atcgtgacaa
     3781 aaaaagcgtt cctcactgta tcgttcatgc ctgcatgaac gggttattct tcctgactac
     3841 agaattacat cgctccttca gttcatacat cacttttaca tcacttatac atcactttta
     3901 catcacttat acatcacttt tacatcattc cttcagctca tacgtcactt tcatcctctg
     3961 tgccatttgt acatcacccc cttcctgaac gtgctgacag cgggttttcc ggctgcgccg
     4021 gcaacgcttc acggccacgc aataaattgc gtccccgttc accgttttcg gatgggaaga
     4081 acgccggaaa caggacttac cccggctgaa tggtgtgaat ttccgcgcta tgcacttgtg
     4141 cgcatactca tgcatgccgt aaaaacagag cctgcgcgtt tctggcgggt tttcgggtgg
     4201 tttgttgcct gttttaccgg tttcccgtca gaaacgccct gagggcctct caggcggtgc
     4261 tgataacaga tgttatggta aatcagagtc acctgcactg ttactctgaa ccggagcgaa
     4321 acagccgcga ccagaatccc cgcttttttt cctgtgccag cgcgtcagcc agacggctaa
     4381 tttcggcctg tagttgttcc ctctcctcat ttcgcaggcg gttttctgct tcccgcgcca
     4441 tcttgtcttc aagcattagg gtaacagtct gctgcagagc gtccagtttc tgctgaatga
     4501 cgtctagctg agcattttcc gcctggctaa cgacagattt ggagtgtccc tccttgtgcc
     4561 tttttggtgt ctcagaagtg cgcagctcgc cataggcccg aatcagttca ctggtctcga
     4621 actcccggcg atcatttttt cctgtgcgat agctgacaag gcctttagcc atgtcacgat
     4681 ataattgcga gcgcgacttt cctgttaatt tcagtgtctc ttgaactgtg tgccatgcca
     4741 taaaagtgac agtgtctcct gtgtgtaccg tattgtaaca gcagaacata ttactgtttt
     4801 aacacaaaag gtctgaggct gcccgtgagg cgtgaaaaat acacaaaaaa cctgttgacg
     4861 gtgtggtttt gacgggcgta gtatttcgaa tactgtatat aaacacattg ttataaatac
     4921 agtttttgct gtttttaaaa gtcaaagagg ataattttat gagtacagag ttaaatagcg
     4981 gtactgtcgg tggttttggt cacatgggac caaatgaccc ctacaatgat ggtcatagcg
     5041 gctctggaag ttcgggaggt agttttaacg gtggtggtaa tggtggccat gataatagta
     5101 gtggttcatc ttatactgca tcctttggtc tcatcactgg ccgacacccg ataactgtcc
     5161 caggtacgaa gaatattaat tctgtcgatc tttctgctgt cattaataac attatacatc
     5221 atgaaggtgc acgcaataac atgtacctag atactgcgaa caatgtaaga gtgggagtga
     5281 cgggcactgg ctggcaatgt ctagcaacgg caggcatttc ggctgagggt aaaagaactt
     5341 tccgctaagc gatagactgt atgtaaacac agtattgcaa ggacgcggaa catgcctcat
     5401 gtggcggcca ggacggccag ccgggatcgg gatactggtc gttaccagag ccaccgaccc
     5461 gagcaaaccc ttctctatca gatcgttgac gagtattacc cggcattcgc tgcgcttatg
     5521 gcagagcagg gaaaggaatt gccgggctat gtgcaacggg aatttgaaga atttctccaa
     5581 agcccgcgac gcagcgccgg caggcagagc aagtagaggg cagcgcctgc aatccatgcc
     5641 cacccgttcc acgttgttat agaagccgca tagatcgccg tgaagaggag gggtccgacg
     5701 atcgaggtca ggctggtgag cgccgccagt gagccttgca gctgcccctg acgttcctca
     5761 tccacctgcc tggacaacat tgcttgcagc gccggcattc cgatgccacc cgaagcaagc
     5821 aggaccatga tcgggaacgc catccatccc cgtgtcgcga aggcaagcag gatgtagcct
     5881 gtgccgtcgg caatcattcc gagcatgagt gcccgccttt cgccgagccg ggcggctaca
     5941 gggccggtga tcattgcctg ggcgagtgaa tgcagaatgc caaatgcggc aagcgaaatg
     6001 ccgatcgtgg tcgcgtccca gtgaaagcga tcctcgccga aaatgaccca aagcgcggcc
     6061 ggcacctgtc cgacaagttg catgatgaag aagaccgcca tcagggcggc gacgacggtc
     6121 atgccccggg cccaccggaa cgaagcgagc gggttgagag cctcccggcg taacggccgg
     6181 cgttcgcctt tgtgcgactc cggcaaaagg aaacagcccg tcaggaaatt gaggccgttc
     6241 aaggctgccg cggcgaagaa cggagcgtgg ggggagaaac cgcccatcag cccaccgagc
     6301 acaggtcccg cgaccatccc gaacccgaaa caggcgctca tgaagccgaa gtgccgcgcg
     6361 cgctcatcgc catcagtgat atcggcaata taagcgccgg ctaccgcccc agtcgccccg
     6421 gtgatgccgg ccacgatccg cccgatatag agaacccaaa ggaaaggcgc cgtcgccatg
     6481 atggcgtagt cgacagcagc gccggccagc gagacgagca agaccggccg ccgcccgaaa
     6541 cgatccgaca gcgcgcccag cacaggtgcg caggcaaatt gcatcaacgc atacagcgcc
     6601 agcagaatgc catagtgggc ggtgacgtcg ttcgagtgaa ccagatcgcg caggaggccc
     6661 ggcagcaccg gcataatcag gccgatgccg acagcgtcga gcgcgacagt gctcagaatt
     6721 acgatcaggg gtctgttggg tttcacgtct ggcctccgga ccagcctccg ctggtccgat
     6781 tgaacgcgcg gattctttat cactgataag ttggtggaca tattatgttt atcagtgata
     6841 aagtgtcaag catgacaaag ttgcagccga atacagtgat ccgtgccgcc ctggacctgt
     6901 tgaacgaggt cggcgtagac ggtctgacga cacgcaaact ggcggaacgg ttgggggttc
     6961 agcagccggc gctttactgg cacttcagga acaagcgggc gctgctcgac gcactggccg
     7021 aagccatgct ggcggagaat catacgcatt cggtgccgag agccgacgac gactggcgct
     7081 catttctgat cgggaatgcc cgcagcttca ggcaggcgct gctcgcctac cgcgatggcg
     7141 cgcgcatcca tgccggcacg cgaccgggcg caccgcagat ggaaacggcc gacgcgcagc
     7201 ttcgcttcct ctgcgaggcg ggtttttcgg ccggggacgc cgtcaatgcg ctgatgacaa
     7261 tcagctactt cactgttggg gccgtgcttg aggagcaggc cggcgacagc gatgccggcg
     7321 agcgcggcgg caccgttgaa caggctccgc tctcgccgct gttgcgggcc gcgatagacg
     7381 ccttcgacga agccggtccg gacgcagcgt tcgagcaggg actcgcggtg attgtcgatg
     7441 gattggcgaa aaggaggctc gttgtcagga acgttgaagg accgagaaag ggtgacgatt
     7501 gatcaggacc gctgccggag cgcaacccac tcactacagc agagccatgt agacaacatc
     7561 ccctccccct ttccaccgcg tcagacgccc gtagcagccc gctacgggct ttttcatgcc
     7621 ctgccctagc gtccaagcct cacggccgcg ctcggcctct ctggcggcct tctggcgctc
     7681 ctgctgcggc gtccgctcgt gggccgtggc gcgggtccgc gcgccggcct cgtgcgcctg
     7741 gcgctcgcgg gcgaggtcca gggcggccgt cttcacgttc tgccttgcgc agatgagata
     7801 gatctagcgt ggactcaagg ctctcgcgaa tggctcgcgt tggaaacttt cattgacact
     7861 tgaggggcac cgcagggaaa ttctcgtcct tgcgagaacc ggctatgtcg tgctgcgcat
     7921 cgagcctgcg cccttggctt gtctcgcccc tctccgcgtc gctacggggc ttccaggggt
     7981 cgtttgcggg aaggggcgga atcctacgct aaggctttgg ccagcgatat tctccggtga
     8041 gattgatgtg ttcccagggg ataggagaag tcgcttgata tctagtatga cgtctgtcgc
     8101 acctgcttga tcgcggccgc gatagctaga tcgcgttgct cctcttctcc atccgcgttc
     8161 caagctgcgg aaaggcaccc ataagcgtac gcctggtcga gcaggcgacg cggatcgacg
     8221 tccagcgcac gagagaatgc gtccgccatc tgtgcaatgc gtctaggatc gagacaaagg
     8281 tcgtctctgt cagccggatc gtagaacata ttggcggcgc caaagcccac ttcaccgacc
     8341 agaccgacgg gatctatcac cagccagccg cgactggaga acatgatgtt ttcatgatgc
     8401 a
//
