LOCUS       NZ_ON470177             8513 bp    DNA     circular CON 23-JUN-2024
DEFINITION  Bacillus thuringiensis strain SY27.1 plasmid pSY27.1_3, complete
            sequence.
ACCESSION   NZ_ON470177
VERSION     NZ_ON470177.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 8513)
  AUTHORS   Karim,A. and Yilmaz,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2022) Department of Enzyme and Microbial
            Biotechnology, Erciyes University, Kosk Mahallesi Fuat Sezgin
            Caddesi, Kayseri 38030, Turkey
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_023648165.1-RS_2024_06_23
            Annotation Date              :: 06/23/2024 04:37:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 9
            CDSs (total)                 :: 9
            Genes (coding)               :: 9
            CDSs (with protein)          :: 9
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            ON470177.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8513
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="SY27.1"
                     /db_xref="taxon:1428"
                     /plasmid="pSY27.1_3"
     gene            complement(join(8328..8513,1..78))
                     /locus_tag="NDN70_RS00005"
                     /old_locus_tag="SY271_000697"
     CDS             complement(join(8328..8513,1..78))
                     /locus_tag="NDN70_RS00005"
                     /old_locus_tag="SY271_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874967.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPNELIKIGISINDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     gene            352..579
                     /locus_tag="NDN70_RS00010"
                     /old_locus_tag="SY271_000698"
     CDS             352..579
                     /locus_tag="NDN70_RS00010"
                     /old_locus_tag="SY271_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869869.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000869869.1"
                     /translation="MKVVSNIRMIMAKKNIDNISDLVRITGVSRNSINKLWHNENVSSL
                     KLDTLITICEKLDVELLDLIEYIRDDSETK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            1051..2130
                     /locus_tag="NDN70_RS00015"
                     /old_locus_tag="SY271_000699"
     CDS             1051..2130
                     /locus_tag="NDN70_RS00015"
                     /old_locus_tag="SY271_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RapH N-terminal domain-containing protein"
                     /protein_id="WP_001102658.1"
                     /translation="MNVQLGNEQITKLLNDWYQEIRAQHIIKAKQLKQKIEKEINNIEE
                     DQNLLLYYSLIDLRYKILTNDYADREQSLEKIEQLKEHTNNFLDYYYHFFKGMHAMKTG
                     NYSETQKYYDIAEKLLEEIPDEVEKAEFNYAVATFYYHTHQALLATQYANKAKSFFSGK
                     LGYEIKTGACENTLGMACITLREFSVAEEYLLSAINKFEKHSENKLALIVRYNLGLLYA
                     DQNLSELAIRYLLESFENQEDDYKTMFLLAQEYYKLKQTNTVNTYIEKGLNVCNQEYKI
                     HFLFLQALNNKLSTEQLEKVMLDAIPYFEKQNLWKYIQDYTEELAIRFYEEKNKDKSNE
                     YFYKSYKAKRNLLERESLK"
                     /name="RapH N-terminal domain-containing protein"
     gene            2130..2273
                     /locus_tag="NDN70_RS00020"
                     /old_locus_tag="SY271_000700"
     CDS             2130..2273
                     /locus_tag="NDN70_RS00020"
                     /old_locus_tag="SY271_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002029250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002029250.1"
                     /translation="MKKFSLILIGVACTTGIFFSQFNNSIQTHDAKEKNDIIQQYAHGK
                     DI"
                     /name="hypothetical protein"
     gene            2384..2974
                     /locus_tag="NDN70_RS00025"
                     /old_locus_tag="SY271_000701"
     CDS             2384..2974
                     /locus_tag="NDN70_RS00025"
                     /old_locus_tag="SY271_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002029249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002029249.1"
                     /translation="MKFMKKISIERSNWRKTYKKMIPVLLVGGILAGNVAFVTNNVFAA
                     TTNNNNIIQTKQSLVSGQDEKSINELAEAFQFMHEEAAIYNNQGEIVDFDFNKIEAKYG
                     NDVGLDALKQQIKKDKSDIAGLSFTSCMKDALYDFFGVNAVQAAFNGGLAYYLEKKLWK
                     EAAKIGVKFFIGSSVAGLAATLAYYGGKCAIYG"
                     /name="hypothetical protein"
     gene            2967..3164
                     /locus_tag="NDN70_RS00030"
                     /old_locus_tag="SY271_000702"
     CDS             2967..3164
                     /locus_tag="NDN70_RS00030"
                     /old_locus_tag="SY271_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000361625.1"
                     /translation="MDKSSILKQMYFILFPVTLFLSLNIYQWIVKSDYSFTGYIIQNLI
                     IVALALVAAIIAYLITKKNI"
                     /name="hypothetical protein"
     gene            4561..5742
                     /gene="mobV"
                     /locus_tag="NDN70_RS00035"
                     /old_locus_tag="SY271_000704"
     CDS             4561..5742
                     /gene="mobV"
                     /locus_tag="NDN70_RS00035"
                     /old_locus_tag="SY271_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003273525.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_002029247.1"
                     /translation="MQKFKMRDVKGLQIHNQREKESHSNSDIIQARTEQNYDLIHDKEK
                     VDYKKLIQNRIDNGVVSNRAIRKDAVVCCSFMISASPDYMNSLSQVEQRRFFEESLRFL
                     KGRYGEENFVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFKNKSEFHDLQDKIYEHV
                     KEKGFDIERGVSSDRKHLSTQRFKAVTLQQEIEELEQEKKGIDSRLHDLKMSLDQAKSV
                     DEIPVKEKGGFIRSKTVEITSEDFESIKVLARSSEALREDVRRLKNESGVITREKDGLY
                     REKCILEGQITELKRENRGLKEENDFFKKTLERVKELYKEKLPELAGVIGYVKGSILDR
                     MNCKFLKRYFTSDDEVRGAQKFLNHKQEHEEQQKRLKQVRSSQQKNRDQGLER"
                     /name="mobV"
     gene            6212..7258
                     /locus_tag="NDN70_RS00040"
                     /old_locus_tag="SY271_000705"
     CDS             6212..7258
                     /locus_tag="NDN70_RS00040"
                     /old_locus_tag="SY271_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085408.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001085408.1"
                     /translation="MAKKNQDEITGKLQPKKKQNIKIYEFIKKKMSESGKELFKSCSIF
                     NEFLATKDKKKKKRVKGNDCKNRFCPICAWRKAGKDAVKIATMMEAIKIEEKKEFLFLT
                     LTTPNIKADMVKSEIDRFNKAFKKLFDRRNIQRSIKGYIRKLEMTYDKERFITEEMYNN
                     KKRKAYYDSRGLKVDDHNPNYDTYNPHFHVLLCVDKNYFKRKELYIKQEEWLEMWREVT
                     DMPEITQVHIQKVELIREGNAVAEVAKYSAKDYEMSVSQEVFDVFYLALKGRQLIVYGG
                     LLKDYAKKYEDGELDKYKSTDKNEYYYRLIAMWNKDLMKFEQEYQELSESEKQEYNGHL
                     INEVDVEA"
                     /name="protein rep"
     gene            7262..7459
                     /locus_tag="NDN70_RS00045"
                     /old_locus_tag="SY271_000706"
     CDS             7262..7459
                     /locus_tag="NDN70_RS00045"
                     /old_locus_tag="SY271_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000861196.1"
                     /translation="MKVAGDLYYYCLGCKKFHEYEKIDHKGVNRKLCFYCFKIQSKKTK
                     IIGDAEGRMQICETCHKELF"
                     /name="hypothetical protein"
     Typing          6188..7240
                     /name="rep_cluster_617"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.934"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          4538..5739
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atatggtttt ttaccaacat atttttctac ataaaagtat ctttttccgt ttgctttgta
       61 atttgttagg aatgccaaaa tgataatctc ctcttagttt ttttagagtt aaaaatacat
      121 tttaaaagat taaattttaa gttatatatt taattttaac tctaaggggt taaaatttca
      181 attaaaacaa ataaaattaa ataataaaac aatatatttt atttttaagg ttaatattaa
      241 atgtgaatat ataaaaaata accttaatat tatttaattt atgttttatt tgtataaaag
      301 tatccttgta agtaaaatgg tatgtacatt gaatgaaaag gagtatgaaa aatgaaggta
      361 gtaagtaata ttcgtatgat tatggcgaaa aagaatatag ataatatttc ggatttggtg
      421 agaataacgg gagtaagtcg taattctatt aataagttgt ggcacaatga gaatgtgtct
      481 tctttaaaat tagatacatt gataacgatc tgtgagaagt tggatgttga gttattggat
      541 ttaattgaat atattcgaga tgatagtgag acaaaataat ttaggggcta ttttagcttt
      601 agatagcttg tgtatgatga aagcatattt ttgctaatcg acaatgagtt atattcacat
      661 atgttttttt tgtagggcta tatgtgtatt taaagttttg atttctattt gttattgtat
      721 gaatatggtt tgaatgtggt aaatgaaatt atagtcttgc taggtgcgtc aacaccttgg
      781 aagatgagcg gtactggcat acttcgtgat ttcattgata gtagtgcagt aaagtgatgg
      841 aattgatgag tattgaaaaa gagttcctgt ttgggggctc tttttttatt tgttactaag
      901 tatttttaaa tagtgaaggt gctacactat ttaaaaatac agaatattaa aaactttata
      961 tatcaactgt tgaaaagtta gtcttataca atagcatgat aacatgatag aaaataaaat
     1021 atagataatt ttggaaaagg agaagattct atgaatgttc aactaggaaa cgaacaaatt
     1081 acaaaattat taaatgattg gtatcaagaa attcgagcgc aacacattat taaagcaaaa
     1141 caattaaaac aaaagattga aaaagaaatt aacaacatag aagaagacca aaatttactt
     1201 ttatattatt cacttattga tttgagatac aagattctta caaacgacta tgcagataga
     1261 gaacaatctt tagaaaaaat cgaacaatta aaagaacaca caaataattt tttagattac
     1321 tattatcatt tctttaaagg catgcatgca atgaaaacag gaaattatag tgaaactcaa
     1381 aaatattatg atatagcaga aaaattacta gaagaaattc ctgatgaagt agaaaaagct
     1441 gaatttaatt atgctgttgc aactttctat tatcatactc atcaagcctt attagcaact
     1501 caatatgcta acaaagccaa aagttttttt agtgggaaac taggatacga gattaaaacc
     1561 ggagcatgtg aaaacacact tggtatggct tgtattacat taagggaatt ttctgttgca
     1621 gaagaatact tgctttccgc aattaataaa tttgaaaaac acagtgaaaa caaattagct
     1681 ttaatcgtac gttataactt aggtctttta tatgcagatc aaaatctttc tgaacttgca
     1741 atccgttatc ttcttgaatc ctttgaaaat caagaagacg attacaaaac aatgttttta
     1801 ttagcacaag aatattacaa gttaaaacaa acaaatacag taaacacata tattgaaaaa
     1861 gggctaaatg tttgtaacca agaatataaa attcatttct tatttttaca agcattaaac
     1921 aataaattat ctactgaaca attagaaaaa gtcatgttag acgcgattcc atattttgaa
     1981 aaacaaaatc tatggaaata catacaagat tatactgaag aattggctat aagattttat
     2041 gaagaaaaga ataaggataa atctaatgaa tatttttata aaagttacaa agctaaaaga
     2101 aacttattag aaagggagtc attgaaatga tgaaaaaatt tagtctgata ctaatagggg
     2161 tagcatgtac aacaggtatt ttcttttccc aattcaataa ttccattcaa acacatgatg
     2221 ctaaagaaaa aaatgatatt attcaacaat atgcacatgg aaaagatatt taaaagcaaa
     2281 ttccaacaat tagcaatcga cctaaatatt aggattatta acaaaatatt gaattatatg
     2341 taaattaagt ttattatcta ttttgaataa taaacttaag gggatgaaat ttatgaaaaa
     2401 aattagtatt gagagaagta attggagaaa aacatataaa aaaatgattc cagtattgtt
     2461 agtcggagga atattggcag gaaatgtagc atttgtgaca aataatgttt ttgcggcaac
     2521 tacaaataac aacaatataa tacaaacgaa acagtctctc gtttctggtc aagatgaaaa
     2581 gagcattaat gaattagcag aagcgtttca atttatgcat gaagaagccg ctatatataa
     2641 taatcaaggt gaaattgttg attttgattt taataaaata gaagcgaaat atgggaatga
     2701 tgttggttta gatgcactta aacaacaaat taaaaaagat aaaagtgata ttgctggttt
     2761 atcttttaca agttgcatga aagatgcttt atatgacttt tttggagtaa atgctgtaca
     2821 agccgcattt aatggtggat tggcatatta tcttgagaaa aaattatgga aagaagcggc
     2881 taaaataggc gttaaattct ttataggaag tagcgtagca ggtttagcgg ctactttagc
     2941 ttattatgga ggtaagtgtg caatttatgg ataaaagttc aattttaaaa caaatgtatt
     3001 ttattttatt tccggtaaca ctttttctat ctctaaatat ttatcaatgg attgttaaaa
     3061 gtgattactc atttacagga tatataatcc aaaatttaat tattgtagca ttagcgttag
     3121 tagcagctat catagcttat ttaattacta aaaaaaatat ttaacacata atgttagtct
     3181 aaataacaga aaattctaac ccttattctt attttgtatt atactggtta tgtacaggta
     3241 aaatggttta caagttaatt tttcctcgtt tcaaaaaaag tccattcttt tcaaaagaat
     3301 ggactttttt cttgttatat ttgctgtaac tattaaaagg gcatcaaaac gatctacaaa
     3361 agcctcaaga cgacccacaa accatcttta aacggtctca aaacgaacta caacccgaaa
     3421 aaacgcatcc cagttttttt aggtttaatt ttaagcgcat accaagcgca tataggcgca
     3481 tacgggacac atacaaagtg ccgaaaaacg catataggtc gcatacaaac ggtagaaata
     3541 catccctaaa aatagagtga tagaaaccca ataggcacag cctatttcac cacttttttt
     3601 gcaaagtggg attcggtttc cttatgctct ttttttattt ttgcttttta agggtcttat
     3661 aatcgttttt aagtatttta ggatgttggg tttcttaagg gaactagaag ttcctagaat
     3721 ggattctgag cgttctgagg tgtatttttc tatggattcg atattggaat tgctctagga
     3781 atagttgtcg ttttattctg tggctgttcc gacagtggtt ctcataaaga atatgattgt
     3841 atcaatagat ttgatgatgt ttgaaaaata gagaatagtt gaagctcttc ctgtaaaaca
     3901 aaaaaaatta tgtttgtaga ggaagtagtc atttcactac aagaaaattc ccatagatcg
     3961 gtggcgagtt gaaaaatgtt gaaggttggc gaggacattt tacgaggacg cgggaatggc
     4021 tttgtcattg ttggggttaa tagtttttaa gaaaagttgg ttagtagctg aaaaaaatag
     4081 ggaagattgg cgaggatgta ttgcatggca gcgggacatg ttatgtagtc gtggtgaata
     4141 taaaaagaga ctttttaata catggatgtc aactgtaaaa agttagaggt agttgtatga
     4201 tgtttgtcca acggtaagcg gaacgaagtg agcattaagg agtggacgaa cccactgtgc
     4261 ccagaaccct cttggaactc caaaacgtta gccgttttgg ggatgccaac cggcgaggga
     4321 gtccctcaag gattgagggg ttggggagtt cgattgaaag gggtcaaggg gaagagtttc
     4381 ccttgtgggt ttgggcaaag cccatgattt tttttgccat gcttgttatg gctcggcggt
     4441 agccgaaatg tcccgaaggg cacacgtgtt tacgcagtaa actgtagtgt gctactgttt
     4501 acatggaact acccccgaaa aaaaggagcg aggcccatgt cgaagttcgt gatttgccat
     4561 atgcaaaaat ttaagatgag agatgtgaaa ggattacaaa tacataatca acgtgaaaaa
     4621 gaaagtcatt ccaattcaga tattattcaa gcacgaacgg aacagaatta tgatctgatt
     4681 catgacaaag aaaaagtgga ttataagaaa ttaattcaaa atcgaattga taatggtgtt
     4741 gtatctaatc gtgcaattcg gaaagacgct gttgtttgtt gttcgttcat gatttcggct
     4801 agtccggatt atatgaattc tttatctcaa gtggaacaga gacgattctt tgaagagtct
     4861 cttcgttttt tgaaggggag atatggagaa gagaactttg tgtatgcttc cgtacatgtt
     4921 gatgaaaaaa cacctcatat gcatgtgggt atggttccag ttaatgaaaa acaaaaacta
     4981 tcagcgtatt cattttttaa aaacaaatca gagtttcatg atttacaaga taaaatctat
     5041 gaacatgtga aagaaaaggg atttgatata gagcgtggtg tttctagtga tagaaaacat
     5101 ttatcgacgc agcggtttaa agcggtgact ttacaacaag aaatagaaga actagagcaa
     5161 gaaaagaagg gaatagatag tcgtcttcat gatttaaaaa tgtctttaga tcaagcaaaa
     5221 agtgtggatg agatcccagt taaagaaaaa ggtgggttta tacgctctaa aacggtcgag
     5281 attacctcgg aagacttcga aagtataaaa gtattagcta ggtcgtcgga agcccttaga
     5341 gaagatgtta gacgccttaa aaacgaatca ggtgtaatta cacgtgaaaa agatgggctg
     5401 tatagagaga aatgtatttt agaggggcaa ataacagaat tgaaaagaga gaatcgagga
     5461 ttgaaggaag agaatgattt ctttaagaaa acgttggaac gagtgaaaga gttgtataaa
     5521 gaaaagcttc ctgaattggc tggggtgatt ggttacgtta aggggagtat attggacagg
     5581 atgaattgca aatttttaaa acgatatttt actagtgatg atgaagtgag aggcgctcaa
     5641 aagtttttga atcataaaca agagcatgaa gaacaacaaa agagattaaa acaagtgcga
     5701 agtagccaac agaaaaatcg ggaccaggga ttggagagat agtgtggttc tgtttgagtt
     5761 ttttgttttt tagatttata gcgttagcgg aatttgtttt ttataacttg cttaagatga
     5821 gtatgtttct cagatgtttt atccgttaaa aaaaatggtt ttgaacgaaa aaaaatgccc
     5881 ctgtccacgg agtggattta ggggcatttt tttatgattt ttcgtgttcg aaaaatcgag
     5941 ttttttcttt ctttaataca taggtaacta tttcggcaac gaaattgatg cctatgtatt
     6001 cttttagccc ttatgtatca agggtttgta aaaaaaaaga cttgcttttt caggcggata
     6061 gatttatcat ttttagtgtc gaattaaaaa tcataaatac cacgagagga gcaagtccaa
     6121 aaaaaggatt cgtacaccct tttttatatc gaatatgaaa ttcagaaatt agaaattctg
     6181 atgtttttca gataggacta atatatcaaa tatggcaaag aaaaatcaag atgaaatcac
     6241 tggtaaatta caacctaaaa agaaacagaa tatcaagatt tacgaattta taaagaagaa
     6301 aatgagtgag agcgggaaag aattgtttaa atcgtgcagt atctttaatg aatttttggc
     6361 tacaaaggat aaaaagaaga agaaacgagt aaaggggaat gattgtaaaa atcgattttg
     6421 tccgatttgt gcttggagga aagcagggaa ggacgcggtg aaaatcgcaa cgatgatgga
     6481 agcaattaag attgaggaaa agaaagagtt tcttttttta acgttgacga ctccaaatat
     6541 taaagctgat atggtgaaaa gtgagattga tagatttaat aaagctttta aaaagttatt
     6601 tgatagacgt aatattcaac gttctattaa gggttatatt agaaagctag aaatgacgta
     6661 tgataaagaa cgattcatca ctgaagaaat gtataacaac aaaaagagaa aagcatatta
     6721 tgattctcgt ggattgaaag tagacgatca taacccgaat tatgatacat ataatcctca
     6781 ttttcatgta ttgctttgtg ttgataaaaa ttattttaaa cgtaaagagc tttatattaa
     6841 gcaagaagaa tggttagaaa tgtggcgaga agtaacagat atgccagaga ttacgcaagt
     6901 acatattcaa aaagtagagt taatacgaga aggaaatgca gttgcggagg ttgcgaagta
     6961 ttcagcgaaa gactatgaaa tgtcggttag tcaagaagtg tttgacgtgt tctatttagc
     7021 attaaaaggt cgtcaattaa ttgtttacgg tggattatta aaagattatg cgaaaaaata
     7081 tgaagatggc gaattggaca agtataagag tacagacaag aatgaatatt attatagatt
     7141 aatcgcaatg tggaataagg atttaatgaa atttgaacaa gagtatcaag agttatctga
     7201 gagtgagaag caagagtata acggtcattt aattaatgaa gtagatgtgg aggcgtagtg
     7261 tatgaaagta gcgggagatt tatattatta ttgtcttggt tgtaaaaagt ttcatgaata
     7321 tgaaaaaatt gatcataaag gtgtaaatag aaaattatgt ttttattgtt tcaagattca
     7381 atctaaaaaa acgaaaatta ttggtgatgc tgaaggtaga atgcaaattt gtgagacttg
     7441 tcataaagaa ttgttttagt tggttgattt gtgaaaaaag caaagtcaaa tgttagaaat
     7501 ttttaataag ggaactgact gttatatcaa gtcagttccc ttattttatt gagtctattt
     7561 ttgagtgcag caagtagcat tcaaaagttt ttttatgatt taacaaaaag gagtttttct
     7621 taaatttaaa tttcgtgaat ttgtaaattt atttttaaag tttatttcga gatatatttt
     7681 gttctactaa ttttgtttta ctaacgtacg aggtgaaaac ttaaagtgtg cgcatgaatt
     7741 tttttagtaa tatatattac gataaaatac cgcacgtttt ttgatgattt ttttgaaaat
     7801 atagttttga gaaattctaa attttcgggg ctgattttgg attttttatg tgggatttga
     7861 ttttggaatt tgttaaattg gaaatctcga tttttcgatt ttcggaattt ttgttttctc
     7921 ttttctttgt tattctgtat ttcgaaatta aggagtttat ataatacctt catgacgaac
     7981 tgaaaacgaa aaaactttgc tcgcatattt aattcataga tatttcatat cgttaaaaag
     8041 ttgcaccgtt tcaaacgagt tgtgtgataa aaagatgttt agattatgtt tgaaattgtt
     8101 gtttctgtga attttttatt tcgcgatttc tgtatatttt taatcctttt tactcttaaa
     8161 atctttttgc ttttgtgcgc aaatgaaaaa atcaaaaagt cacgcgtaac ttttattaaa
     8221 tatataaata tattattaaa atacctaacc cttttatgat gaaaaaatat aaatagttaa
     8281 agaaaaaaga ctgataaaaa atatcagtct tttttcttta gttttattta taaagagtat
     8341 ctctttatgg tgttttctac tttttctctc cagttctcta tatcgttaat tgatattccg
     8401 atctttatta attcgtttgg aatgaaactg ttatctaaaa tccataaagt gagcctttct
     8461 aatgtgattc tttcgtttcc aattgaataa atacgttcac tttgtttgca agt
//
