LOCUS       NZ_ON209487             9294 bp    DNA     circular CON 26-MAR-2024
DEFINITION  Klebsiella pneumoniae strain MDR119 plasmid pCol119, complete
            sequence.
ACCESSION   NZ_ON209487
VERSION     NZ_ON209487.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Parker,J.K., Gu,R., Estrera,G.A., Kirkpatrick,B., Rose,D.T.,
            Mavridou,D.A.I., Mondy,K.E. and Davies,B.W.
  TITLE     Shared Genetics of Carbapenem-resistant and ESBL-producing
            Enterobacterales in Central Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Parker,J.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2022) Molecular Biosciences, The University of
            Texas at Austin, 2506 Speedway, Austin, TX 78712, United States
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Geneious Prime v. 2021.1.1
            Sequencing Technology        :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028737675.1-RS_2024_03_26
            Annotation Date              :: 03/26/2024 01:15:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 11
            CDSs (total)                 :: 11
            Genes (coding)               :: 11
            CDSs (with protein)          :: 11
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            ON209487.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="MDR119"
                     /isolation_source="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCol119"
                     /geo_loc_name="USA"
                     /collection_date="2019"
                     /note="carbapenem resistant"
     gene            join(9211..9294,1..174)
                     /locus_tag="PWB68_RS00005"
                     /old_locus_tag="AGG42_00005"
     CDS             join(9211..9294,1..174)
                     /locus_tag="PWB68_RS00005"
                     /old_locus_tag="AGG42_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            259..408
                     /locus_tag="PWB68_RS00010"
                     /old_locus_tag="AGG42_00010"
     CDS             259..408
                     /locus_tag="PWB68_RS00010"
                     /old_locus_tag="AGG42_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            1889..3853
                     /locus_tag="PWB68_RS00015"
                     /old_locus_tag="AGG42_00020"
     CDS             1889..3853
                     /locus_tag="PWB68_RS00015"
                     /old_locus_tag="AGG42_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            3853..4584
                     /locus_tag="PWB68_RS00020"
                     /old_locus_tag="AGG42_00025"
     CDS             3853..4584
                     /locus_tag="PWB68_RS00020"
                     /old_locus_tag="AGG42_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            4591..5121
                     /locus_tag="PWB68_RS00025"
                     /old_locus_tag="AGG42_00030"
     CDS             4591..5121
                     /locus_tag="PWB68_RS00025"
                     /old_locus_tag="AGG42_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5149..5328)
                     /locus_tag="PWB68_RS00030"
                     /old_locus_tag="AGG42_00035"
     CDS             complement(5149..5328)
                     /locus_tag="PWB68_RS00030"
                     /old_locus_tag="AGG42_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5397..5774
                     /locus_tag="PWB68_RS00035"
                     /old_locus_tag="AGG42_00040"
     CDS             5397..5774
                     /locus_tag="PWB68_RS00035"
                     /old_locus_tag="AGG42_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(5836..6135)
                     /locus_tag="PWB68_RS00040"
                     /old_locus_tag="AGG42_00045"
     CDS             complement(5836..6135)
                     /locus_tag="PWB68_RS00040"
                     /old_locus_tag="AGG42_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6125..6388)
                     /locus_tag="PWB68_RS00045"
                     /old_locus_tag="AGG42_00050"
     CDS             complement(6125..6388)
                     /locus_tag="PWB68_RS00045"
                     /old_locus_tag="AGG42_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(6694..7113)
                     /locus_tag="PWB68_RS00050"
                     /old_locus_tag="AGG42_00055"
     CDS             complement(6694..7113)
                     /locus_tag="PWB68_RS00050"
                     /old_locus_tag="AGG42_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            7516..9201
                     /locus_tag="PWB68_RS00055"
                     /old_locus_tag="AGG42_00065"
     CDS             7516..9201
                     /locus_tag="PWB68_RS00055"
                     /old_locus_tag="AGG42_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             2516..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(512..1090)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3854..4581
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 gatggttctg tcattgaaag tctggggatg cctttaaagg ataatattaa taatggttgg
       61 tttgatgttg aaaaatcatg ggtttcgata ttacagccac actttaaaaa tgtaatcgat
      121 attagtaaat ttgattactt tgtatccttt gtttaccggg atggtaactg gtaagcaaaa
      181 gttatcaagg atgatttgaa atacagactt ccggtgttca gggatgaata ccggggtttt
      241 taaatacagg gaaggtatat gaaaaaggta aaggttgttt ttctttttat attgattttt
      301 tcaggctttc ttctggtcgc atgtcaggca aactatatcc gggatgttca gggtggaacg
      361 gtggcaccat cgtcctcctc tgaactgacg gggatcgcgg ttcagtagaa aagatcaaag
      421 gatcttcttg agatcctttt tttctgcgcg taatcttttg ccctgtaaac gaaaaaacca
      481 cctggggagg tggtttgatc gaaggttaag tcagttgggg aactgcttaa ccgtggtaac
      541 tggctttcgc agagcacagc aaccaaatct gtccttccag tgtagccgga ctttggcgca
      601 cacttcaaga gcaaccgcgt gtttagctaa acaaatcctc tgcgaactcc cagttaccaa
      661 tggctgctgc cagtggcgtt ttgcgtgctt ttccgggttg gactcaagtg aacagttacc
      721 ggataaggcg cagcagtcgg gctgaacggg gggttcttgc ttacagccca gcttggagcg
      781 aacgacctac accgagccga gataccagag tgtgagctat gagaaagcgc cacacttccc
      841 gcaagggaga aaggcggaac aggtatccgg gaaacggcag ggttggaaca ggagagcgca
      901 agagggagcg attcatcgga aacggtgatg atctttaagt cctgtcgggt ttcgccactc
      961 ctgactgatt catggttgag ccaccggctc ccacagatgc accgaaaaag cgtctgttta
     1021 tgtgaactca gtcaggaggg cggagcctat ggaaaaacgc cacctgcgtg gccttgccgt
     1081 tttgcctcac atgttagtcc cctgcttatt cacggaatct gtgggtaact ttgcatgtgt
     1141 ccgcagcgcc cgccgcagtc tcacggccgg agcgtagcga ccgagtgagc gaggaggcgc
     1201 aatttccccg gcacctacaa gacggacacc ggacgcgctg atgtggcata ttgtcccctg
     1261 attaagtctt tatattccct gataataaat taccaggata ggtgaatcat atcggttttt
     1321 cgctgaacat tttacgttaa cctatctctg ttgctttttt ctccttacgg agaaatacag
     1381 gtcatccccg cgagggcgtg gctgacctca gggggatatc ccctgcttac gctggctgga
     1441 cgccaccgac gcacggtctc tccccctctt tctggctccg tccggctgga cgccggccac
     1501 gccagaaatt cacccggccc ccgtcaggct cacgcctgac cacccacaaa aatccacggt
     1561 gctggacgca cctggcgaag cagcaaagct gctcctgatt ttgcgggtgg tcagccgctc
     1621 gctatggcga actctgacgg gggccggaac accggaaaag accgcactgg ggtgccgcct
     1681 tttcctctca cacgctgggg cgtgctcgtt gttcctccgg ggctgcgcct tttccgcatg
     1741 gggcggaaag aggctggcct ccgtggtgaa agctgaacgc aatcaccacg gacgtgaccg
     1801 ccgccgccat gccttggggc atgtccgcgg cttgtgcagc gggggctgca catcacgccg
     1861 gcaaaccatc gtctgaagga gagagaacat gtttaatacg gattcgcttg cctggaagtg
     1921 gcacattccg gagctggccc ggcagattta tccgtgggtc agctggctgt accaggagta
     1981 ttacggtctg gccggggctt tcctagttat cggcctgttt ttcggtctgg ctgaactttg
     2041 ggccggacgg atgcgttttg tggggctgag tcataaggag cgacgggagt atgccgatcg
     2101 ctataagcgc gataataaac ggccagcgcc attttcggtg ctggcctcgc tggattcgcc
     2161 cgggcggatg gtcagggcac gtcgacatgt ctggaactac gcgtactggc tgaccggcgc
     2221 ggccggtctg gcccttctgg gggggctggt tgtcgccgtg tttatcgaga gctggggaga
     2281 aaccgtcctg cgtccggacc tctacgcaaa cgcagattat tacctgctgc agccggctaa
     2341 cctgttcggg attgcctggt gcgcgggcgg gctggctgtc gggattgtgt tggcgcggct
     2401 gctggcgttc ctgctgcttg acggtcattt tgcggctgct gacgaggcgg tgaacgcgaa
     2461 actgaatcag gagagccggc gcagcagcca gcggacagga gagatgaccg acgtgcgcca
     2521 cctgcatttc ggggagccgg taccggttaa tgcgctggcc gatttttcct cggagcaggc
     2581 ccggcaggag caggcggtgt ttctgggtaa ggatgagcag ggcaagccgg tgctggttcc
     2641 gcgtgatacc tggcgcaaga ccaacattca gatcctcggc ctgcccggga gcggcaagag
     2701 cgtgatggcc accaacgcgc tcattcgctg cgtacgcgat ttcggggacg cggtggtgta
     2761 cttcgacccc aaaggggacg catgggcgcc gcacgtcttc cgggcgcact gtccggactt
     2821 caccctgctg gatctgcggc cggggaaacc ggcgcagctg aatctgttcc gcgatctcga
     2881 ccagtacgcg ctgaaaaacc tgctgattgc cgggcttaac ctgggcgaga ccggcgacgt
     2941 ggcggaccac taccgcatca gcgagcagaa agcggcgaag ctgattgcgg agcagtttcc
     3001 gcagggggca aacattcagc aggtgctggc ggcggcgtac gcgctgccgg aggccctgaa
     3061 aaaggacgtg aaggggttga tcactaggct ggaaaacgtc gctgacctga gcgtcctgca
     3121 gacggacagt ggcatcgatg tggcggggat tatcaacggc ggcggctgcc tgtacgttat
     3181 cggctcgatg gacgacgagg cggtgatccg tgtgcaaaaa atgctgtttg cccgctgcgc
     3241 gcaaatcatc attgcgcggg acgagttccg ggagtggcca cacgtcagcg tgatgctcga
     3301 tgaaattaaa tatctgctgt cgaagtatgt actgaacgca ctggggacgc tgcgcagccg
     3361 ggactgcaat ctgttgctcg cgcaccagtc gctgggcgat ttcgggcagt gcgggcagga
     3421 tctgcccgct gaatttgtga aaaccacggt actggataac acgccgatcc gctggtttta
     3481 ccgggcggcc agcctggagt ccgcgcagtg ggcggcgggg cagaccgggg aaatcagggt
     3541 ggacgtggag cgccgccgtg ccagccggga ggcggggaac gtggagcata tcagcgggga
     3601 cagcttcatc cagaaggagg cgcggccgct gtttgacgtg aacaccctgc aacacctgcc
     3661 ggacgggttc gcggtcatga ccgggctggg tgtggcccgg atggcgttca gcagtccgct
     3721 gcgggttgag cgccgggaaa tcccgttgca gcgtttcccg gtgctggcga aaaccgatcc
     3781 cctggcggag ttccggcccc cggccgacgg cggcgggccg aaagcggatg atgattttgc
     3841 ggggatttac tgatggcgct ggagctgtac aacgtcagcc acgcgaaacg acaggcgcgg
     3901 aacgcggaga aaacccggct gaccctgcgc tggttgcggg aagagctgtg ttccacggcg
     3961 gagctggttg cgcggcggct ggggattgcg gcggtgcagc cggtctaccg cttcctcgac
     4021 agcctggtgg cgaaagggct gctggtccgg accaaatatc cggtggacgg ccgtcaggtc
     4081 agcgtgtggg ggctgacgcc gcatggcgtg gcgttctcgt tcgacgagga cgaaccgctg
     4141 gcggatgtta ttccgtttca gccgtcaagg gtgtcggcag cacatctgcc gcaccggctg
     4201 gccgtgcaat ccctgcgcct tgatatggaa gccagagggg ccaccggctg gcgcattctg
     4261 caccgtctga gtcttaaagg catgaaagtg ccggacgccc tggcggagct ggacggtaaa
     4321 acggtggcgt ttgaggtgga gcgcaccgtc aagagccgtc gccggtatca ggaagtggtg
     4381 tccgggtatc tgttcaaccg caaagctaac ggcatcgatg aaatctggta catctgcccg
     4441 gaccgggcga cgcaggtcag ggtgcagcgg gcaatcctgt cggtggatga aatcgtgaac
     4501 ccacagaccg gcgaggcccg caaaacggca gagctggatc gtgaacggtt gtttgcgtgc
     4561 tttaaattta tgacaacgga gtgagggatt atggcgctgt acatggtatt tattgaagac
     4621 gcggaggacc ggaacggcga gcgctgcatc atgccgacca gcgagcatct ggaagatatg
     4681 gtggtgctcg cctggcagac cggggcgtat gttttctgtt ttgtcgaccg ggcccagcat
     4741 ctggcgatga tgaaatccat cgggatgtat gacgagcggc tcaacagcga cgaaaaagcc
     4801 acgtactttt tcactggtaa aaatccgcaa tgggaacgga acaaacccac aggaatgaag
     4861 ctgtgtgatt acctctatcg caaggtggag cggttcgcca tcgagaacgg tgtgcaggtg
     4921 gttgatgccg ggaagtgctc accggagcag attaacgcga tgattgacgt ggtgagtgag
     4981 cggcacgggg aaagcgcggt gaacgagaag ctgaaagcgg agctgatgcg ggaatataac
     5041 agccgctatt ttaacgtgcc ggtacctgaa ctgccggagg acaaacctgc ccgccggtgg
     5101 tggccgttcg gcaaaaaata aatgccagca ccggccatgc cggtgctgtt attcctcccc
     5161 gagcttatcc cgcatcacct gccagagtgt ttccgcatcc tcgtgtagcc gctcgcacat
     5221 gtcggccggg gtatccatat ccagctcatt cagtttatcg agcaatagca gcgactgctc
     5281 cttaataaat ttagccatat tgagggctgt tttttcctgt tttgtcatga ttctttcctt
     5341 ccgggcatgg tgtgcgcctg tcaggtatac tgtctcaaaa atagcggggt gagacaatgg
     5401 cgtgggtgac ggtgagacag gcgacggaac tgacgggtaa agcccgaagt tccctgtatc
     5461 gggatatggc gaaaggccgc gtatcatacc gtacggaagc tgatggcggc cgggtggtgg
     5521 ataccagcga gcttatccgg gtgtacggtg agttaagaca ggatgagact cacgagcggg
     5581 acgagttgag actgtcgggt gagactgaaa aaatggcctc cgacgcgttg atcgcggaga
     5641 taaaagcact cagggaggag gtggccgggc tacggcagga aatgcagtcc atgagactgc
     5701 tggaacacaa accggagaca gcagagaccc cggccggaac accgcgccgg tggtggccgt
     5761 ggggaaaacg ctgacgcgct gtgcttgtcc aaccggcgac ggccacgcaa tgaattgcgt
     5821 ccccgtcacc ggttattaga gaacaatatg gggattctgg cgctggccga ccacggccag
     5881 aacccggact tttctggtct ggtcatcaac atcatagagg acatgctgtc ccatcagact
     5941 gatccggcga acgtcctcgc catagcgtga gtccacttca tagagtcgcg ggaagtcggc
     6001 cagtttgtcc gccgcagcga gaacccggtc ggtaacgttc tgcgcatccc acagggatga
     6061 attttccgcg atatagtcaa acagcgtcgt tacatcctcg atggctccct gcttccattc
     6121 cacgtcatac ggcatcgcgc agttcctgat aacggccatg caggcgctgg cggagttgtt
     6181 ccgtggtcat acccggacgc gtgtcagcac gggcggcggt cactttggcg cgaacctgat
     6241 cttcatactc tgcctgggcc agttcttcgt tagccagtcg ctgcaacagc gcagctttgt
     6301 ccgggccacc ttccggcagt atgcggggca ggctttcagc cagttcccga agaaagttgg
     6361 cgttttcgta gtgtgtagtg ttgctcattg cggagcctcc cgggttaatg cggcaatatg
     6421 aaggaagttt agcagggggc ggtctgaaag aatatggtcg tgtcagtcag gctgatgaag
     6481 cagaaagtcg gtaaaaaagg gcttacggca tccattttac gtcaaaacat atgccatgca
     6541 gttgcgtgca taggcatgca ttaggataaa atttatcggg cgcgtttccg gcagttttcc
     6601 gggggggttg ttgcctgttt tatcccgtag ccgccggaaa cgccctgagc ccgtctgagc
     6661 ggtgcgcgta atgacgcgtt atggtaaatc cacttactgt ctgccttcat agccatcgag
     6721 ataaacagct gaaatggtgt cagccagtag ccttgatttt ttcggtgtga ggtctttgct
     6781 gcggtaatcg gtacccgcaa attgtgttgt gccttggagt gagtttaaaa attcctgact
     6841 gtgaaactgc tgaatgcgtt gctgcacatc actccgtgat tttccggtcg ctttatcctt
     6901 tgcgcccata tcccagaact gccggaaaaa aggcaccatc attcgtgttg tgtcagcttt
     6961 ctgggtggaa aaattgggat caaaaccgtc atctgaagca tatacgtaat gccgggtatg
     7021 ctgttccacg ctgccctttg gagcacaggc agtgagagtt gctgtcgctg ttatcatggc
     7081 gataagacac agtttgctgc cggaacgtct catgagcgtc tccttacctt taattctttt
     7141 ttctcattga gttatatatc cgcaggctat cttatcatca gtttctgacc gtgttcagtg
     7201 accgaacaac ccgaaccaga ccccccgttt cttttcctgt cccggagggg gctttctggt
     7261 atctcatata atgatttcag taaaacatac tgaggccgga gaacggcgcc gtttgtgctg
     7321 gtttttgttg catctggagg ggattgcgca tggtgtgagt ggctgttttt ttgatcattt
     7381 tcacaaaaac gcaaatttac agacttgaca tgaaaaacaa ggcagcgtaa tttttatact
     7441 gtatataaaa ccagtggtta tatgtacagt attttatttt agtttattgt tttaaaagtc
     7501 aaagaggaaa agtatatgag tggcggagac ggacgaggtc cgggtaattc aggtctggga
     7561 cacaatggtg gtcaggccag tgggaatgtg aacggtacat caggtaaagg tggcccttca
     7621 tcaggtggtg gtacggatcc aaacagcggg ccgggctggg gtacgacgca tacacctaac
     7681 ggggatattc ataactataa cccgggggag tttggtaacg gcgggagtaa acccggtggt
     7741 aatggcggta acagcggcaa tcatagcggg agctctggtg gcggacagtc ttcggccacc
     7801 gcgatggcct tcggtctgcc tgccttggcc actccggggg ctgaaggact ggctttatcc
     7861 gtttccggcg atgcgttgtc ggccgctgtt gctgatgtgc tggctgccct gaaaggtccg
     7921 tttaagtttg gtctgtgggg gattgcgatt tacggagtgc tgccttctga gatagcaaaa
     7981 gatgatccga aaatgatgtc aaaaatcgtg acgtcattgc cggcagacac ggtaacggag
     8041 acgccggtca gctccctgcc gctggaccag gcgacggtca gcgtcactaa acgtgtggcg
     8101 gatattgtga aggacgagcg gcagcatatt gcggttgtca ccggccggcc aatgagtgtt
     8161 cctgtggtgg atgcgaaacc gacaaaacgt ccgggggtat tcagtgtgtc gattccgggt
     8221 ctcccgtctc tgcaggtgag cgtacctaaa ggtgttccgg ccgcgaaagc cccgccaaaa
     8281 ggcattattg ctgaaaaagg tgattcacgt ccggctggtt ttacggccgg tggtaactcc
     8341 cgtgaagccg ttattcgttt cccgaaagag agcggacaga aaccggtcta tgtgtcggtg
     8401 acggatgttc ttactccggc acaggtaaaa cagcgtctgg aggaagaaaa gcgtcgccag
     8461 caggcatggg acgctgctca cccggaagag gggctgaaaa gagagtatga taaagcgaaa
     8521 gctgagctgg atgctgaaga taaaaatatt gcgaccttaa acagtcgcat tgcatcgaca
     8581 gagaaggcga tccccggtgc aagggctgct gttcaggaag ccgataaaaa ggtaaaagag
     8641 gcagaggcaa ataaagatga ttttgtgact tataacccgc ctcatgaata tggctccggg
     8701 tggcaggatc aggttcgcta tcttgataag gatattcaga atcagaatga gaaattaaaa
     8761 gctgcccaga catctttaaa cgaaatgaat gaatccttat ccagggataa ggctgcgctt
     8821 tccggggcga tggagagccg gaaacaaaag gagaaaaaag cgaaggatgc agaaaataaa
     8881 ttaaatgagg aaaagaaaaa acctcgtaag ggaactaaag attacggcca tgattatttt
     8941 cctgatccca agactgaaga tattaagggg ttgggagagt tgaaagaggg taaacctaaa
     9001 acccctaaac aaggtggtgg tggtaagcgg gcccgatggt atggtgataa aaagcgtaaa
     9061 atttatgaat gggattccca gcacggtgag cttgaagggt accgcgccag tgatggcgaa
     9121 cacctcgggg cattcgatcc aaaaacgggt aagcaggtta aagggccgga tccaaaacga
     9181 aacattaaaa aatatcttta agaggtaaat atggggctta aattacatat tcattggttt
     9241 gataagaaaa ccgaagagtt taaaggtggt gaatactcaa aagacttcgg tgat
//
