LOCUS       NZ_OM735811             2989 bp    DNA     circular CON 02-OCT-2023
DEFINITION  Escherichia coli strain EW659 plasmid pEW659-QnrB, complete
            sequence.
ACCESSION   NZ_OM735811
VERSION     NZ_OM735811.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Furlan,J.P.R., Stehling,E.G., Savazzi,E.A., Peixe,L. and Novais,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) Department of Clinical Analysis, Toxicology
            and Food Science, School of Pharmaceutical Sciences of Ribeirao
            Preto - University of Sao Paulo, Avenida do cafe, s/n, Ribeirao
            Preto, Sao Paulo 14110-000, Brazil
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v.3.9
            Assembly Name                     :: pEW659-QnrB
            Sequencing Technology             :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/02/2023 12:29:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3
            CDSs (total)                      :: 3
            Genes (coding)                    :: 2
            CDSs (with protein)               :: 2
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OM735811.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EW659"
                     /isolation_source="Water"
                     /db_xref="taxon:562"
                     /plasmid="pEW659-QnrB"
     gene            <714..869
                     /locus_tag="RUR87_RS00005"
                     /pseudo=""
     CDS             <714..869
                     /locus_tag="RUR87_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            963..1607
                     /locus_tag="RUR87_RS00010"
     CDS             963..1607
                     /locus_tag="RUR87_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     gene            complement(2616..2765)
                     /locus_tag="RUR87_RS00015"
     CDS             complement(2616..2765)
                     /locus_tag="RUR87_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162846302.1"
                     /translation="MPSEYDEQRGSTDTQLPGEYDLFDLMAKVTEEILHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     ARG             964..1604
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(2801..2974)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          2..455
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.868"
                     /coverage="81.0"
                     /evalue="1.14e-179"
ORIGIN
        1 ggtcgttcgg ctgcggcgag cggtgccggc ttacaggcgg ggcggaaatt tcctgggaga
       61 taccaggaag gagcttaaca gggaagagat aaggccgcgg cgaagccgtt tttccatagg
      121 ctccgccccc ctgacaaaca tcatgaaatc tgacgctcaa atcagtggtg gcgaaacccg
      181 atgcggctta aagataccag gcgtttcccc ctggtggctc cctcgtgctc ccccgcattc
      241 ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttatct cattccacgc
      301 ctgacactca gttccgcgaa ggcagttcgc tcctggatgg actgtatgca cgaacccccc
      361 gttcagtccg actgctgcgc cttatccggt aactatcatc ttgagtccaa cccggaaaga
      421 cacgacttat cgccactggc agcagccact ggtaactgat ttagaggagt tagtcttgaa
      481 gttatgcgcc tgttaaggct aaactgaaag aacagatttg gttactgcgc tcctcgtaag
      541 ccagttacca cggttcaaag agttgctgct ctgagaacct tcgaaaaacc gccttgcagg
      601 gcggtttttt cgtttacaga gcaagagatt acgacgccac agaaaggatc tcaagaagat
      661 ccttcaccgt aatggaaatc tatccaatcc gagcacatag gatacacatc gttataacgc
      721 tggatctgcg tgaattccag cagcagcaag agaaagactt tctacaaacc agcttacagc
      781 aggctaaatt taaccagaaa aaagccgctg aattactggg tctgacttat catcagctac
      841 gtgccttgct gaagaaacac cagatctaat acgcatttgc aggtgtcata ctggcgggtt
      901 tgacgcataa cgtcataagg tttaccatgg cgtcattact gtataaaaac acaggcatag
      961 atatgactct ggcattagtt ggcgaaaaaa ttgacagaaa tcgcttcacc ggtgagaaag
     1021 ttgaaaatag tacatttttt aactgcgatt tttcaggtgc cgacctgagc ggcactgaat
     1081 ttatcggctg ccagttctat gatcgcgaaa gtcagaaagg gtgcaatttt agtcgcgcaa
     1141 tgctgaaaga tgccattttc aaaagctgtg atttatcaat ggcagatttc cgcaacgtca
     1201 gtgccttggg cattgaaatt cgccactgcc gcgcacaagg cgcagatttc cgcggtgcaa
     1261 gctttatgaa tatgatcacc acgcgcacct ggttttgcag cgcatatatc actaatacta
     1321 atctaagcta cgccaatttt tcgaaagtcg tgttggaaaa gtgtgagctg tgggaaaacc
     1381 gctggatggg gactcaggta ctgggtgcga cgttcagtgg ttcagatctc tccggcggcg
     1441 agttttcgac tttcgactgg cgagcagcaa acttcacaca ttgcgatctg accaattcgg
     1501 agttaggtga cttagatatt cggggtgttg atttacaagg cgttaagtta gacagctacc
     1561 aggcatcgtt gctcatggag cggcttggca tcgctgtgat tggttagttc agcgtttaca
     1621 ccgggagatg cgtttgacga tttactttta aggggttcct gtatctaatt ttttgctgga
     1681 cattcatacc cgcgatcatc gcgtttattg agtttattat ttatctatgc gattccgatg
     1741 aaaaattcgc acgaaaatat ggctaactca tagcaaaaaa aaagccccct aagggctttt
     1801 ttttgtacct gacggtcaga ccgattaaac ataacatccc ttgcgtgcac cgcctgagag
     1861 gctctgaggg cgtttctgac gggaaaccgg taaaacaggc aacaaaccac ccgaaaaccc
     1921 gccagaaacg cgcaggctct gtttttacgg catgcatgag tatgcgcgca agtgcatagc
     1981 gcggaaattc acaccattca gccaggggaa gtcctgtttc cggcgttctt cccatccgaa
     2041 aacggtgaac ggggacgcaa tttattgcgt ggccgtgaag cgttgccggc gcagccggaa
     2101 aacccgctgt cagcacgttc aggaaggggg tgatgtacaa atggcacaga ggatgaaagt
     2161 gacgtatgag ctgaaggaat gatgtaaaag tgatgtataa atgatgtaaa agtgatgcaa
     2221 aagtgttgta aaagtgatgt acaggccgaa ggagtgttgt aaaagtgttg taaaagtgat
     2281 gtatgaactg aaggagcgat gtaattctgt agtcaggaag aataacccgt tcatgcaggc
     2341 atgaacgata cagtgaggaa cgcttttttt tgtcacgatc tccccttaac ctgcgtaaat
     2401 cacccgccat ggttgattat ttttcaacct caaaatggtc atacacctaa cgcagccgcg
     2461 attttcatcg caactgcatg aattaaaaga aaaaaaagta atgtttcgtt ttcacgagta
     2521 atacccaggt attaaaaacc cactgtaatt acatgaaaat aaagaaataa aaggtaatac
     2581 cggcgtatta ccacgtacgc gggcaagcga aaaagctata acgcttcccg gccgacaggc
     2641 ggcccagaat ccacaggggc atgcaggatc tcttcagtaa ccttcgccat taaatcaaaa
     2701 aggtcatatt cgccgggaag ctgcgtatca gtcgaacccc gttgttcatc atattcagat
     2761 ggcatggggt gatatccagt agcagagagg ttgttagcgg gtttcggggc gcagccctga
     2821 accagtcacg tagcgctagc ggagtgtata ctggctgact atgttggcac ggatgtggaa
     2881 atcagtgaag tgcttcatgt agcaggagaa aaaaggcggc accagcgcgt cagcaggata
     2941 tgtagtacag gatatattcc gcttcctcgc tcactgactc gctgcgctc
//
