LOCUS       NZ_OM144978            10060 bp    DNA     circular CON 25-SEP-2023
DEFINITION  Klebsiella pneumoniae strain F94 plasmid pB, complete sequence.
ACCESSION   NZ_OM144978
VERSION     NZ_OM144978.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Wang,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2022) Department of Central Laboratory, Municipal
            Hospital Affiliated to Taizhou University, No.381-1,Zhongshan East
            Road,Jiaojiang, Taizhou, Zhejiang 318000, China
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. v0.4.5
            Sequencing Technology        :: nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 09/25/2023 00:41:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 13
            CDSs (total)                 :: 13
            Genes (coding)               :: 13
            CDSs (with protein)          :: 13
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OM144978.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="F94"
                     /db_xref="taxon:573"
                     /plasmid="pB"
                     /geo_loc_name="China: Taizhou"
                     /collection_date="2021"
     gene            join(8941..10060,1..845)
                     /locus_tag="N4J74_RS00005"
     CDS             join(8941..10060,1..845)
                     /locus_tag="N4J74_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            845..1576
                     /gene="mobC"
                     /locus_tag="N4J74_RS00010"
     CDS             845..1576
                     /gene="mobC"
                     /locus_tag="N4J74_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            1583..2113
                     /locus_tag="N4J74_RS00015"
     CDS             1583..2113
                     /locus_tag="N4J74_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2140..2319)
                     /locus_tag="N4J74_RS00020"
     CDS             complement(2140..2319)
                     /locus_tag="N4J74_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2345..2773)
                     /locus_tag="N4J74_RS00025"
     CDS             complement(2345..2773)
                     /locus_tag="N4J74_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(2937..3251)
                     /locus_tag="N4J74_RS00030"
     CDS             complement(2937..3251)
                     /locus_tag="N4J74_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3239..3517)
                     /locus_tag="N4J74_RS00035"
     CDS             complement(3239..3517)
                     /locus_tag="N4J74_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(3692..4057)
                     /locus_tag="N4J74_RS00040"
     CDS             complement(3692..4057)
                     /locus_tag="N4J74_RS00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(4054..4425)
                     /gene="tolA"
                     /locus_tag="N4J74_RS00045"
     CDS             complement(4054..4425)
                     /gene="tolA"
                     /locus_tag="N4J74_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(4699..4944)
                     /locus_tag="N4J74_RS00050"
     CDS             complement(4699..4944)
                     /locus_tag="N4J74_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            5271..6956
                     /locus_tag="N4J74_RS00055"
     CDS             5271..6956
                     /locus_tag="N4J74_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            6966..7223
                     /locus_tag="N4J74_RS00060"
     CDS             6966..7223
                     /locus_tag="N4J74_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            7308..7457
                     /locus_tag="N4J74_RS00065"
     CDS             7308..7457
                     /locus_tag="N4J74_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             271..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(7561..8139)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          846..1573
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 caaacattca gcaggtgctg gcggcggcgt acgcgttgcc ggaggccctg aaaaaggacg
       61 tgaaggggct gatcaccaag ctggagaacg tggccgacct gagcgtcctg cagacggaca
      121 gcggtatcga tgtggcgggg ataatcaacg gcggcggctg cctgtacgtc atcggctcga
      181 tggacgatga ggcggtgatc cgggtgcaga agatgctgtt tgcccgctgc gcgcaaatca
      241 tcattgcgcg ggatgagttc cggaggtggc cgcacgcgag catcatgctc gatgagatta
      301 aatatctgct gtcgaagtac gtgctgaacg cgctggggac gctgcgcagc cgggactgca
      361 acctgctgct cgcgcaccag tcgctgggcg acttcggtca gtgcgggcag gatctgcccg
      421 ctgattttgt gaaaaccacg gtactggata acacgccgat ccgctggttc tatcgcgcgg
      481 ccagccagga gtccgcgcag tgggcggcgg gccagaccgg ggagatccgg gtggacgtgg
      541 agcgccgccg ggccagccgg gaggcgggga acgtggagca catcagcggc gacagcttca
      601 tccagaagga ggcgcggccg ctgtttgacg tgaacaccct gcaacacctg cctgacgggt
      661 tcgcggtgat gaccgggctg ggcgtggcgc ggctggcgtt cagcagcccg ctgcgggttg
      721 agcgccggga aatttcgctg aaaaccttcc cggtgctggc gaaaacggac ccgctggcgg
      781 agtacctgcc tccggccgac ggcggcggac caaaagcgga tgatgatttt gcggggcttt
      841 actgatgaca ctggagcagt acaacgtcag ccacgcgaag cggcaggcgc ggaacgcgga
      901 gaaaacacgg ctgaccctgc gctggctgcg ggaagagctg tgttccacgg cggagctggt
      961 tgcgcgacgg ctggggattg cggcggtaca gccggtttac cgcttcctgg acagtctggt
     1021 ggcgaaaggg ctgctggtcc gggcgaaata cccggttgac ggccgtcagg tcagcgtgtg
     1081 ggggctgacg ccgcacgggg tggcgttctc gttcgacgag gacgagccgc tgacggacgt
     1141 tattccgttt cagccgtcaa gggtgtcggc ggcgcagctg ccgcaccggc tggccgtgca
     1201 gtccctgcgc ctctccatgg agggccgggg ggccaccggc tggcggtacc tgcaccgtat
     1261 ggccctgaaa gggatgaaag tgccggacgc gctggcggag ctggacggca gaacggtggc
     1321 atttgaggta gagcgcaccg tcaagagccg caggcggtat caggaagtgg tgtccgggta
     1381 tctgttcaac cgccgggcga acggcatcga tgaaatctgg tacatctgcc ctgaccggcc
     1441 gacgcttgtc cgggtacagc gggcgattct ggcggtggat gaaatcgtga atccacagac
     1501 cggcgaggcc cgcaaaacgg tggagctgga ccgggagagg ctgttcgcgt gctttaaatt
     1561 tatgacaacg gagtgaggga ttatggcgct gtacatggta tttattgagg acgtggaaga
     1621 caaagacggc gagcgctgca ttatgccaac cagcgagcat ctggaagaca tggtggtgct
     1681 cgcctggcag accggggcgt atgttttctg ttttgtcgac cgggcccagc atctggcgat
     1741 gatgaaatcc atcgggatgt atgacgagcg gctcaacagc gacgaaaaag ccacgtactt
     1801 tttcaccggt gaaaatccgc aatgggaacg ggacaaaccc acaggaatga agctgtgtga
     1861 ttacctctat cgaaaggtgg agcggttcgc catcgagaac ggtgtgcagg tggttgatgc
     1921 cgggaagtgc tcaccggagc agattaacgc gatgattgag gtggtcagcg agcggcacgg
     1981 ggaaagcgcg gtgaacgaga agctgaaagc ggagctgatg cgggaatata acagccgcta
     2041 ttttaacgtg ccggtgcctg aactgccgga ggacaaaccc gcccgccggt ggtggccgtt
     2101 cggcaaaaaa taacgccagc accggcatgg ccggtgctgt tattcttccc cgagcttatc
     2161 ccgcatcacc tgccagagtg tttccgcatc ctcgtgaagc cgttcgcaca tgtcggccgg
     2221 ggtatccatg tccagctcgt tcagcttttc cagcaacagc agcgactgat ccttaatgaa
     2281 tttagccata ttgagggcag ttttttcctg cttcgtcatg ttctccattc ccttcggtaa
     2341 gttctcagct aaaccagccg gaccagaagc gttttttctt ctgtgtcagt tctgttgtca
     2401 atctgtccac ttcggcctgg agtttccggc gctcatcatc gtgctggcgg cgttcttctt
     2461 cccgggatgt tttatcttcc agcatcaggg tgacagcctg ccggagatcg gccagttcct
     2521 tttgcattgc cagaagctgt tcctgtggga aatttttttg tgtattttca cggtgtgaaa
     2581 tgccgggaat ttgtttctca gggatattca catttccgta tacacggatc agttctgaca
     2641 catccacgac agggttgttt ttcccgtcac gtgacacggt aacctttccc tgttttacat
     2701 gattgtatag cgttcttctg gtaatgcccg cggcttgtgc tgcctgtgaa aggttgagca
     2761 atgttttcgc cactgtatac ccctgatttt atcgggtgtg aaaggtgtgt aatttctcac
     2821 ggtaaatgta tcgctgaatt ttacccgttt ttccgttcag gatgtggttt tctgacgcgc
     2881 tgcgcttgtc aaaccggcga cggccacgca atgaattgcg cccccgtcat cggttattag
     2941 aggctgtctg cccccggata cccggattct ttctgatgcc tgacggtgac gatcacaatc
     3001 tggtccattt cccggtcgtg gcggtagagc atcacatagc cactgtcgcc aaacccgatc
     3061 accagttcct tatattccag cggcagaaac ggcaccgggc ggccaatgtc tggcaatgtt
     3121 ttcagttgct ggatagcccg gacgatcacc tcacctcctt ttctggctgc cagccgattt
     3181 ttggttttga gaaagtcctg aaggcgttgt aaatcgcgct gtgccagcgc ggaaatcgtt
     3241 acctgtggca tggcggagca tcctgctcgt tctcagttcc ccaggtactt atccaggctt
     3301 ccgcttcttc cgcagtaagg tgcagacccg tttcctgata gtgctgccat gcggcctgtc
     3361 cgtcccgcag gtactggctg cgcttctctt cccggtcgat gtattcggtg attgcctcca
     3421 gcattagcgc gtgcgctgag cggtgccggt catcggccag cgttttcagc cggtccttaa
     3481 gttcttcatc gagcctgatg gatgttgctg atgacataaa agcccctttg tagtcatatg
     3541 tattacacat gactacttta atccgtggcg gcgctatttg ccagaaatac ggcttttgct
     3601 gcccattttt cagcttcgct tccactaatc agggctgcta caggctcact catcatccag
     3661 gactattgca gcctgcgggt taaaggacct gttactggcg gcaaccatca tcattgagct
     3721 tttgggtggt gactggtcgc ggatcctgta agccgtcatt tttcagactg aattcaaaat
     3781 ttataacctg tcgcattgcc gggtgccctg cgggatatag ccagtgcctc attgccgtca
     3841 gcgtttcccg aaaaaaaagg tgacagccgg taagcgacac gttgtccact cgtccatcgt
     3901 ccagaatatc aaacgtggcg gtcacctgac cttcgatgcg ttttgctgcg gcctgtaccg
     3961 ggtaggttgg ctgcagtgtg gttacgggtt ttagtggtat cgcatgtacc ggagtgagga
     4021 acatggccgc cagcactgtc agagtaaaag ctttcatggt ctgaaatcca gaggggcgtt
     4081 tttgaatatt tgccaggtat tttcatccgg tgccggcgga attttggcgt gggtaacggc
     4141 tgatattgct gctttacaga gtttcggatc tcctccttca gcaatggcgc tgttcagagt
     4201 gccatcacgt tgtatggata tccgcacggt gcacattttt cccgcatagg cctctggttc
     4261 aaacatctgc atttcaacag cggtcttgat ggccgcagcg tagctactga ttttagatga
     4321 cttatccaga gtataaattt tcggcggctg ggtggtggaa tgcttacagg ccgtcaggag
     4381 taaggggctg cacaatgcca gcaggataaa gggtattaaa cgcatcgggt tgatgacctg
     4441 tcagtaaaaa atgttacatg tatatttgtc actctttacg gccacgttca tttacataaa
     4501 cacggcttat cgtatcgttt ttacaccaaa cggcccgcca tgcagttgcg tgcataggca
     4561 tgcagcgggt tacaatttac cgggcgcgtt tccggcggtt ttccgggtgg tttgttgcct
     4621 gttttatccc gtagccgccg gaaacgccct cagaccgtct gagcggtgcg ggcaatgcga
     4681 cgttatggta aatacccctt accggcggcg ctgtggctgc caggtgaagg tggtttccgt
     4741 agcctcgtat ttctcccgac gcatttcctg cgcccgcctg gcttcacgtt cgtcctgaat
     4801 ggcacgaagc accctttccc gcacctgttg ttttaccgcc tttggcagcg ttttgttccc
     4861 tttcaggccc atggccagtg cctgagcgac aaaggcgtcc gccaggcgct tttcttcagc
     4921 gggagtcagt ttcaggtgcg tcatagtggc ctccgggcgt ctcaggtttc ccgaacagtt
     4981 taaccgcagg tcctctcctc atgccagtta gctttcagat gtgtctatac catctgaaat
     5041 gtaagtgtga tgaatgtctt aaataataaa atttgttgcc atgagaaatt ttcgggaaaa
     5101 gacgcgatct gcttacaggt ggctccgggc gcttttttgt gcctgaagat gagaactaat
     5161 ctcatatatt gacacctgaa aacgggagga gtaaggtaat aattatactg tgtatatgta
     5221 cagtatgttg gctttaattt attgttttaa atgtcaaaga ggaaaacggt atgagtggtg
     5281 gagacggtcg aggtccgggt aattcaggtc tgggacataa tggtggtcag gccagtggga
     5341 atgtgaacgg tacgtctggt aaaggtggcc cttcatcagg tggtggtacg gatccaaaca
     5401 gcgggccggg ctggggtacg acgcatacgc cgaatggaga tattcataac tataatccgg
     5461 gggagtttgg taacggcggg agtaaacccg gtggtaatgg tggtaacagc ggcaatcata
     5521 gcggtagctc tggtggcgga cagtcttcgg ccactgcgat ggccttcggt ctgcctgccc
     5581 tggctactcc gggggctgaa ggactggctt tatcagtttc cggcgatgcg ttgtcggccg
     5641 ccgttgctga tgtgctggct gccctgaagg ggccgtttaa gtttggtctg tgggggattg
     5701 cgatctacgg tgtgctgcct tctgagattg caaaagatga tccgaaaatg atgtcaaaaa
     5761 ttatgacgtc attaccggcc gatgcggtga cggagactcc ggcaagtact ttaccactgg
     5821 accaggcgac ggttcgtgtc agacaacggg ttgtggatgt ggtgaaggat gagcggcagc
     5881 atattgcggt tgtcgcaggt cggccaatga gtgtccctgt ggtggatgcg aaaccgacaa
     5941 aacgtccggg ggtattcagt gtgtcgattc cgggtctccc gtctctgcag gtgagcgtac
     6001 cgaaaggtgt tccgacagcg aaagccccgc caaaaggcat tgttgctgaa aaaggtgatt
     6061 cacgtccggc tggttttaca gccggtggta actcccgtga ggccgttatt cgtttcccga
     6121 aagagaccgg acagaagccg gtttatgtgt cggtgacaga tgttcttacc ccggcacagg
     6181 taaaacagcg tcaggaggaa gaaaagcgtc gccagcaggc atgggacgcc gctcatccgg
     6241 aagaggggct gaaaagagac tatgataaag cgaaagctga gctggatgcc gaagataaaa
     6301 atattgcgac cttaaacagc cgcattgcat cgacagagaa ggcgctcccc ggtgcaaggg
     6361 ctgctgttca ggaagccgat aaaaaggtaa aagaggcaga ggcaaataaa gatgattttg
     6421 tgacttataa cccgcctcat gaatatggct ccgggtggca ggatcaggtt cgctatcttg
     6481 ataaggatat tcagaatcag aatgagaaat taaaagcggc tcaggcatct ttaaacgcaa
     6541 tgaatgaatc cttatccaga gataaggctg cacttaccgg ggcgatggag agccggaaac
     6601 aaaaggagaa aaaagcgaag gatgcagaaa ataagttaaa tgaggaaaag aacaagcctc
     6661 gcaaggggac taaagactac ggccatgatt atcatccagt acctaaaaca gaagacataa
     6721 agggacttgg agagttaaag cgaggtgatc ctaaaacacc taaacaaggt ggtggtggta
     6781 agcgggcccg atggtatggc gataaaaaac gtaaaattta tgaatgggat tcccagcacg
     6841 gtgagcttga agggtatcgt gccagtgatg gcgaacacct aggcgcattt gatcccaaaa
     6901 cgggtaagca ggttaaaggg ccagatccaa aacggaacat taaaaaatat ctttgagagg
     6961 taaatatggg gcttaaatta cgtttgacat ggtttgataa gaaaactgaa gagtttaaag
     7021 gggaagagta ttctaaagac tttggtgacg atggttctgt cattgaaagc ttgggaatgc
     7081 ctttaaagga taatattaat aatggtgagt ttgatttgaa gaaagaatgg gttcctttac
     7141 tacagccatt ttttaaaaat caaataaaac ctgatgtgga taattgttat atagcttttg
     7201 attatcggga cggtaactgg taacaaaaaa ttatcaagga tgatttgaaa tactgacttc
     7261 cggtgttcag ggatgaatgc cggagtcttt aaatacaggg aaagtatatg aaaaaggtaa
     7321 agactatttt tctttttata ttgattgttg ctggctttct tctggccgca tgtcaggcaa
     7381 attatatccg ggatgttcag ggcggaacgg tggcaccatc gtcctcctct gaactgacgg
     7441 ggatcgcggt tcagtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7501 aatcttttgc cctgtaaacg aaaaaaccac ctggggaggt ggtttgatcg aaggttaagt
     7561 cagttgggga actgcttaac cgtggtaact ggctttcgca gagcacagca accaaatctg
     7621 tccttccagt gtagccggac tttggcgcac acttcaagag caaccgcgtg tttagctaaa
     7681 caaatcctct gcgaactccc agttaccaat ggctgctgcc agtggcgttt tgcgtgcttt
     7741 cccgggttgg actcaagtga acagttaccg gataaggcgc agcagtcggg ctgaacgggg
     7801 agttcttgct tacagcccag cttggagcga acgacctaca ccgagccgag ataccagtgt
     7861 gtgagctatg agaaagcgcc acacttcccg caagggagaa cggcggacag gtatccggta
     7921 aacggcaggg tcggaacagg agagcgcaag agggagcgac ctaccggaaa cggtggggat
     7981 ctttaagtcc tgtcgggttt cgcccgtact gtcagattca tggttgagcc tcacggctcc
     8041 cacagatgca ccgaaaaagc gtctgtttat gtgaactctg gcaggagggc ggagcctatg
     8101 gaaaaacgcc accggcgcgg ccctgccgtt ttgcctcaca tgttagtccc ctgcttatcc
     8161 acggaatctg tgggtaactt tgtatgtgtc cgcagcgccc gccgcagtct cacgcccgga
     8221 gcgtagcgac cgagtgagcg aggaggcgca atttccccgg cacctgcaag acggtcaccg
     8281 gacgcgctga tgtgggatat tgtcccctga ttaagtcttt ctattccctg acgatcaatt
     8341 atcaggatag gtgaaccatg tcggtttttc gctgaacatt ttacgttaac ctatctctgt
     8401 tgcttttttc tccttgcgga gaaatgcaag tcatccccgc tggggcgtgg ctgacttcag
     8461 gggaaaagac cttgcttacg ctggctggac gccaccgacg cacggtcttt tcccctcttt
     8521 ctggctctgt ccggctggac gccggccacg ccagaaattc acccggcccc cgtcaggctc
     8581 acgcctgacc acccacaaaa atccacggtg ctggacgcac ctgacgaagc agcaaagctg
     8641 ctcctgattt tgcgggtggt cagccgctcg ctatggcgaa ctctgacggg ggccggaaca
     8701 ccggaaaaga ccgcactggg gtgccgcctt ttcctctcac acgctggggc gtgctcgttg
     8761 ttcctccggg gctgcgcctt ttccgcatgg ggcggaaaga ggctggcctc cgcggtgaaa
     8821 gctgaacgca atcaccacgg acgtgaccgc cgccgccatg ccttggggca tgtccgcggc
     8881 ttgtgcagcg ggggctgcgc atcacgccgg taaacccgtc gtctgaagga gagagattgc
     8941 atgtttaata cggattcgct tgcctggcag tggcatatcc cggagctggc ccggcagatt
     9001 tacccgtggg tcagctggct gtaccgggag tattacagtc tggccggttt ttttctggtt
     9061 atcggcctgt ttttcggtct ggctgaactc tgggccggcc gaatgcgttt tgtggggctg
     9121 agccataagg cgcggcggga gtatgccgat cgctataagc gcgataataa ccggactgtg
     9181 ccattttcgg tgctggcctc gctggattca cccgggcgga tggtcagggc acggcgacat
     9241 gtctggaact acgcgtactg gctgaccggc gctgccggtc tggcccttct gggggggctg
     9301 gttgtcgccg tgtttatcga gagctgggga gaaaccgtcc tgcgcccgga cctctatgcg
     9361 aacgcagatt attacctgct gcagccggct aacctgttcg ggatagcctg gtgtgccgga
     9421 gggcttgtcg ccggcgtcct tctggcgcgg ctgctggcgt tcctgctgct tgatggtcat
     9481 tttgcggccg ctgacgaggc ggtgaacgcg aaactgaatc aggagagccg gcgcagcagc
     9541 cagcggaccg gagaaatgac agacgtccgc cacctgcatt tcggggagcc ggtaccggtt
     9601 aacgcgctgg ccgatttttc cacggagcag gcccggaaac agcaggcggt gtttctgggt
     9661 aaggatgagc agggccagcc ggtgctggtc cctcgtgata cctggcgcaa gaccaatatt
     9721 cagatcctcg gcctgcccgg gagcggcaag agcgcgatgg ccaccaacgc gctcattcgc
     9781 tgcgtacgcg atttcgggga cgctgtggtg tacttcgacc ccaaagggga cgcgtgggcg
     9841 ccgcacgtct tccgggcgca ctgcccggac ttcaccctgc tggatctgcg gccggggaaa
     9901 ccggcgcagc tgaatctgtt ccgcgatctc gaccagtacg ccctgaagaa tctgctggtt
     9961 gccgggttta acctgagtga gaccggcgac gtggcggacc actaccgcat cagcgagcag
    10021 aaagcggcga agctgattgc ggagcagttc ccgcaggggg
//
